Human mesenchymalstemcellviability,proliferationanddifferentiation potential inresponsetoceramicchemistryandsurfaceroughness

Abstract

We investigatedtheeffectoftheceramicchemistryandsurfaceroughnessofpure α-tricalcium phosphate,andalso αTCP dopedwitheither 1.5 wt%or3.0wt%ofdicalciumsilicate(C2S), ontheresponseofadulthumanmesenchymalstemcells(ahMSCs). AhMSCs wereplatedonto ceramic discs,preparedbyasolid-statereaction.Afterbeingsintered,somesampleswerepolishedupto1 μm, whileotherswerekeptas manufactured, whichresultedintwosurfaceroughnessgrades.Viability,proliferationandosteoinductivecapacityweredeterminedfollowing various incubationperiods. The resultsshowedanon-cytotoxiceffectafteranindirectapoptosistest.Celladhesionandproliferationweresurfaceroughness-sensitiveand increased proportionallytotheroughnessofmaterials.Theseobservationsbecamemoreevidentintheunpolished αTCP ceramicdopedwith 1.5 wt%C2S, whichinducedosteoblasticdifferentiationasaresultoftheroughnessandincreasedconcentrationoftheC2S solidsolution in αTCP