Reproductive success through high pollinator visitation rates despite self incompatibility in an endangered wallflower

PREMISE OF THE STUDY: Self incompatibility (SI) in rare plants presents a unique challenge—SI protects plants from inbreeding depression, but requires a sufficient number of mates and xenogamous pollination. Does SI persist in an endangered polyploid? Is pollinator visitation sufficient to ensure reproductive success? Is there evidence of inbreeding/outbreeding depression? We characterized the mating system, primary pollinators, pollen limitation, and inbreeding/outbreeding depression in Erysimum teretifolium to guide conservation efforts. METHODS: We compared seed production following self pollination and within- and between-population crosses. Pollen tubes were visualized after self pollinations and between-population pollinations. Pollen limitation was tested in the field. Pollinator observations were quantified using digital video. Inbreeding/outbreeding depression was assessed in progeny from self and outcross pollinations at early and later developmental stages. KEY RESULTS: Self-pollination reduced seed set by 6.5× and quadrupled reproductive failure compared with outcross pollination. Pollen tubes of some self pollinations were arrested at the stigmatic surface. Seed-set data indicated strong SI, and fruit-set data suggested partial SI. Pollinator diversity and visitation rates were high, and there was no evidence of pollen limitation. Inbreeding depression (δ) was weak for early developmental stages and strong for later developmental stages, with no evidence of outbreeding depression. CONCLUSIONS: The rare hexaploid E. teretifolium is largely self incompatible and suffers from late-acting inbreeding depression. Reproductive success in natural populations was accomplished through high pollinator visitation rates consistent with a lack of pollen limitation. Future reproductive health for this species will require large population sizes with sufficient mates and a robust pollinator community

No-deal food planning in UK Brexit

Even for a country with a long history of arguments about food supply, the UK's present situation is remarkable. A country that received 28% of its food in 2018 directly from the European Union (EU), plus 11% more through EU trade deals, is now planning, under the leadership of Prime Minister Boris Johnson, to leave the EU (“Brexit”) on Oct 31, 2019, with or without an agreement on how and what the terms are for trade, customs, and food security. The food implications for consumers and public health of a no-deal Brexit are seeping out of government but deserve full scrutiny

Heterogeneity in Surface Sensing Suggests a Division of Labor in Pseudomonas aeruginosa Populations

The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP in response to surface adherence. Current thinking in the field is that once cells attach to a surface, they uniformly respond by producing c-di-GMP. Here, we describe how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells. One subpopulation has elevated c-di-GMP and produces biofilm matrix, serving as the founders of initial microcolonies. The other subpopulation has low c-di-GMP and engages in surface motility, allowing for exploration of the surface. We also show that this heterogeneity strongly correlates to surface behavior for descendent cells. Together, our results suggest that after surface attachment, P. aeruginosa engages in a division of labor that persists across generations, accelerating early biofilm formation and surface exploration

Outflows and Massive Stars in the protocluster IRAS 05358+3543

We present new near-IR H2, CO J=2-1, and CO J = 3-2 observations to study outflows in the massive star forming region IRAS 05358+3543. The Canada-France-Hawaii Telescope H2 images and James Clerk Maxwell Telescope CO data cubes of the IRAS 05358 region reveal several new outflows, most of which emerge from the dense cluster of sub-mm cores associated with the Sh 2-233IR NE cluster to the northeast of IRAS 05358. We used Apache Point Observatory (APO) JHK spectra to determine line of sight velocities of the outflowing material. Analysis of archival VLA cm continuum data and previously published VLBI observations reveal a massive star binary as a probable source of one or two of the outflows. We have identified probable sources for 6 outflows and candidate counterflows for 7 out of a total of 11 seen to be originating from the IRAS 05358 clusters. We classify the clumps within Sh 2-233IR NE as an early protocluster and Sh 2-233IR SW as a young cluster, and conclude that the outflow energy injection rate approximately matches the turbulent decay rate in Sh 2-233IR NE.Comment: 15 figures, 42 pages, accepted for publication in the Astrophysical Journal. Full size figures are included at http://casa.colorado.edu/~ginsbura/iras05358.htm. Data can be accessed from figshare: http://figshare.com/articles/IRAS_05358_3543_Data_Cubes/80631

Non-axisymmetric instabilities of neutron star with toroidal magnetic fields

The aim of this paper is to clarify the stabilities of neutron stars with strong toroidal magnetic fields against non-axisymmetric perturbation. The motivation comes from the fact that super magnetized neutron stars of $\sim 10^{15}$G, magnetars, and magnetized proto-neutron stars born after the magnetically-driven supernovae are likely to have such strong toroidal magnetic fields. Long-term, three-dimensional general relativistic magneto-hydrodynamic simulations are performed, preparing isentropic neutron stars with toroidal magnetic fields in equilibrium as initial conditions. To explore the effects of rotations on the stability, simulations are done for both non-rotating and rigidly rotating models. We find the emergence of the Parker and/or Tayler instabilities in both the non-rotating and rotating models. For both non-rotating and rotating models, the Parker instability is the primary instability as predicted by the local linear perturbation analysis. The interchange instability also appears in the rotating models. It is found that rapid rotation is not enough to suppress the Parker instability, and this finding does not agree with the perturbation analysis. The reason for this is that rigidly and rapidly rotating stars are marginally stable, and hence, in the presence of stellar pulsations by which the rotational profile is deformed, unstable regions with negative gradient of angular momentum profile is developed. After the onset of the instabilities, a turbulence is excited. Contrary to the axisymmetric case, the magnetic fields never reach an equilibrium state after the development of the turbulence. This conclusion suggests that three-dimensional simulation is indispensable for exploring the formation of magnetars or prominence activities of magnetars such as giant flares.Comment: 19 pages, 11 figures, to be published in A&

Human resident gut microbe Bacteroides thetaiotaomicron regulates colonic neuronal innervation and neurogenic function

BACKGROUND AND AIMS: As the importance of gut-brain interactions increases, understanding how specific gut microbes interact with the enteric nervous system (ENS), which is the first point of neuronal exposure becomes critical. Our aim was to understand how the dominant human gut bacterium Bacteroides thetaiotaomicron (Bt) regulates anatomical and functional characteristics of the ENS. METHODS: Neuronal cell populations, as well as enteroendocrine cells, were assessed in proximal colonic sections using fluorescent immunohistochemistry in specific pathogen-free (SPF), germ-free (GF) and Bt conventionalized-germ-free mice (Bt-CONV). RNA expression of tight junction proteins and toll-like receptors (TLR) were measured using qPCR. Colonic motility was analyzed using in vitro colonic manometry. RESULTS: Decreased neuronal and vagal afferent innervation observed in GF mice was normalized by Bt-CONV with increased neuronal staining in mucosa and myenteric plexus. Bt-CONV also restored expression of nitric oxide synthase expressing inhibitory neurons and of choline acetyltransferase and substance P expressing excitatory motor neurons comparable to those of SPF mice. Neurite outgrowth and glial cells were upregulated by Bt-CONV. RNA expression of tight junction protein claudin 3 was downregulated while TLR2 was upregulated by Bt-CONV. The enteroendocrine cell subtypes L-cells and enterochromaffin cells were reduced in GF mice, with Bt-CONV restoring L-cell numbers. Motility as measured by colonic migrating motor complexes (CMMCs) increased in GF and Bt-CONV. CONCLUSION: Bt, common gut bacteria, is critical in regulating enteric neuronal and enteroendocrine cell populations, and neurogenic colonic activity. This highlights the potential use of this resident gut bacteria for maintaining healthy gut function

Processes controlling the fate of chloroethenes emanating from dnapl aged sources in river-aquifer contexts

This work dealt with the physical and biogeochemical processes that favored the natural attenuation of chloroethene plumes of aged sources located close to influent rivers in the presence of co-contaminants, such as nitrate and sulfate. Two working hypotheses were proposed: i) Reductive dechlorination is increased in areas where the river-aquifer relationship results in the groundwater dilution of electron acceptors, the reduction potential of which exceeds that of specific chloroethenes; ii) zones where silts predominate or where textural changes occur are zones in which biodegradation place preferentially takes place. A field site on a Quaternary alluvial aquifer at Torelló, Catalonia (Spain) was selected to validate these hypotheses. This aquifer is adjacent to an influent river and its redox conditions favor reductive dechlorination. The main findings showed that the low concentrations of nitrate and sulfate due to dilution caused by the input of surface water diminish the competition for electrons between microorganisms that reduce co-contaminants and chloroethenes. Under these conditions, the most bioavailable electron acceptors were PCE and metabolites, which meant that their biodegradation was favored. This led to the possibility of devising remediation strategies based on bioenhancing natural attenuation. The artificial recharge with water that is low in nitrates and sulfates may favor dechlorinating microorganisms if the redox conditions in the mixing water are sufficiently maintained as reducing and if there are nutrients, electron donors and carbon sources necessary for these microorganisms