Respuesta de anticuerpos séricos en cerdos frente a una vacuna recombinante contra Taenia solium

Evalúa la respuesta inmune en cerdos, tras la administración de la vacuna recombinante desarrollada con el antígeno Tsol18 expresado en el virus de Newcastle (VNC). Para lo cual se usa 15 cerdos, divididos en 3 grupos de vacunación con 5 individuos cada uno: el grupo I por vía ocular, el grupo II por vía nasal y el grupo III (control), solo recibe suero fisiológico. Los tres grupos son vacunados en la semana 0, 1, 5 y 6. Los cerdos son sangrados cada semana por 8 semanas, para la obtención del suero. Los anticuerpos contra el Virus de Newcastle, son obtenidos mediante la prueba de inhibición de la hemaglutinación (HI). El ELISA indirecto estandarizado con el antígeno Tsol18, permite monitorear la presencia de anticuerpos circulantes contra la proteína Tso18 en cerdos inmunizados por vía oral y nasal. Todas las muestras resultan positivas a HI para VNC. Se concluye que no existe diferencia estadística entre la vía de inoculación y los títulos de anticuerpos producidos en los grupos de estudio.Tesi

Valoración comparativa de la macrofauna de lombrices en sistemas agroforestales de café orgánico y convencional en contraste con cultivos en pleno sol y bosque, durante la época lluviosa y seca en Turrialba, Costa Rica

Evalúa la macrofauna de lombrices en sistemas agroforestales con café bajo sistemas de manejo convencional y orgánico durante la época lluviosa y seca. También se estudiaron tres tipos de suelo diferentes (bosque secundario, cañal y pastizal). Las evaluaciones se realizaron en el ensayo de sistemas agroforestales con café, establecido en 2000 en la finca experimental del CATIE en Turrialba, Costa Rica.Tesi

Bovine tuberculosis and the endangered Iberian lynx.

We report the first case of bovine tuberculosis in a free-living Iberian lynx (Lynx pardina), an extremely endangered feline, from Doñana National Park in Spain. The isolate (Mycobacterium bovis) correlates by molecular characterization with other isolates from wild ungulates in the park, strongly suggesting an epidemiologic link. Mycobacterium bovis infects many animal species, with wild and free-ranging domestic ungulates being the main reservoirs in nature (1)

Procesos geomorfológicos activos en cárcavas del borde del piedemonte norte de la Sierra de Guadarrama (Provincia de Segovia, España)

Dos localizaciones situadas cerca del borde del piedemonte norte de la Sierra de Guadarrama (provincia de Segovia) ofrecen formas erosivas en cárcavas bien desarrolladas: (a) un conjunto de barrancos encajados sobre arenas arcósicas del Mioceno, en un relieve de lomas y vaguadas del suroeste provincial; (b) cárcavas sobre arenas silíceas del Cretácico Superior, en un relieve de laderas de mesas y cuestas de la zona centro sur de la Provincia. En este trabajo se caracteriza la actividad geomorfológica de esos barrancos y cárcavas, y se inicia su cuantificación. El objetivo está encaminado a conocer qué procesos movilizan sedimentos, y a evaluar sus tasas de actuación, intensidad, frecuencia de ocurrencia y conectividad entre los mismos. Los procesos de erosión hídrica por salpicadura, arroyada laminar y concentrada en rills, junto con la actividad gravitacional observada, movilizan materiales desde las cabeceras de las cárcavas hasta los colectores, rellenándolos; éstos son evacuados mediante procesos de encajamiento en los canales efímeros, y sedimentados en pequeños conos aluviales. En los barrancos sobre arcosas, los eventos de precipitación poco intensos y de alta frecuencia tienden a rellenar los lechos arenosos, mientras que la fusión nival y las precipitaciones de alta intensidad y baja frecuencia vacían los canales, sedimentando en zonas apicales de los conos aluviales. En las cárcavas sobre arenas silíceas, los primeros datos obtenidos apuntan a unas tasas de erosión y producción de sedimentos muy elevadas, ya que sobre ellas se produce escorrentía y erosión hídrica de manera casi instantánea; por ello, los eventos de precipitación de baja intensidad son capaces de sedimentar en las zonas apicales de los conos, mientras que los eventos de alta intensidad originan lóbulos telescópicos sobre conos aluviales existentes en pedimentos. [ABSTRACT] Two areas located at the edge of the North piedmont of the Guadarrama Mountains (province of Segovia) show a conspicuous development of gullies. A series of valley side gullies, or ravines, cut on arkosic sand sediments of Miocene age at the Río Chico Valley (southwest of the Segovia province); and slope gullies, cut on silica sand, shale and gravel sediments of Upper Cretaceous age, on the slopes of a set of mesas and cuestas at the río Cega piedmont (Pedraza region, south centre of the Segovia province). This paper characterizes the geomorphic activity of both types of gullies. In addition, the quantification of their current activity is initiated. The objectives are: to know which processes are eroding and mobilizing the sediments within the gullies, and to evaluate their rates, intensity, frequency and connectivity. A detailed field survey shows that weathering processes (sandstone sheeting, and the formation of popcorn structures on shale layers), and splash, sheet and rill erosion, along with sand and mud falls, slides and flows move sediment from the gully slopes and internal divides to the dry washes. These materials are temporary stored in ephemeral stream channels (filled with sandy bed material), evacuated by stream-bed scour and fill processes, and deposited in alluvial cones. In order to get a first approximation of the amount of erosion and sediment movement, a set of field methods have been applied to these areas. These methods include: erosion pins, measurement of pedestals, system of rods and washers, pit traps (box and gabion check-dam types), and topographic surveys after extensive sedimentation on alluvial cones, along with the installation of pluviographs. The results show how on the arkosic valley side gullies, the precipitation events of low intensity and high frequency tend to fill the sandy stream-beds, whereas the snowmelt and the high intensity and low frequency precipitation events scour the sandy stream-beds. The sedimentation occurs here at the apex part of the alluvial cones, which rest directly on the nearby floodplain of the Chico River. On the silica sand slope gullies, the first gathered data point at very high rates of erosion and sedimentation (44.1 tons/ha for a single event), which is interpreted due to the fact that runoff and water erosion occurs here almost instantaneously after precipitation. Here, the precipitation events of low intensity and high frequency produce sedimentation at the apex part of the alluvial cones, whereas the high intensity and low frequency precipitation events sediment in the form of telescopic lobes, on existing alluvial cones, which rest on pediments

Health-related behaviors and symptoms of anxiety and depression in Spanish nursing students: an observational study

Background: Symptoms of anxiety and depression are prevalent among young adults and are a significant public health issue. College students are at a higher risk of experiencing poor mental health than other young people due to several factors, including moving away from home, financial pressures, heavy workload, poor time management skills, competitivity, new processes of socialization and insufficient coping mechanisms, among others. In addition, nursing students’ mental and physical health may also be affected by unhealthy lifestyle habits and health-related behaviors. Aim: To determine the prevalence of symptoms of depression and anxiety, and the association between these symptoms and health-related behaviors, in a population of Spanish student nurses. Methods: Cross-sectional study on a sample of 339 nursing students. We used the Hospital Anxiety and Depression Scale, the International Physical Activity Questionnaire-Short form, the Spanish Healthy Eating Index and an “ad hoc” questionnaire for sociodemographic variables. The association between psychological symptoms and health-related behaviors was analyzed through binary logistic regression models. Results: The prevalence of symptoms of depression and anxiety was 3.8% and 24.5%, respectively. Negative health-related behaviors were frequent, namely tobacco and alcohol consumption, suboptimal physical activity and diet. Moderate physical activity was associated with a lower probability of experiencing depressive symptoms. Symptoms of anxiety were related to a low level of physical activity, an unhealthy diet and alcohol consumption ≥2 times a week. Conclusion: Student nurses could benefit from the implementation of both physical and mental health promotion initiatives

Manejo de la focalidad neurológica aguda en reanimación. A propósito de un caso.

La focalidad neurológica aguda en pacientes postquirúrgicos suele suponer un reto diagnóstico, especialmente en pacientes jóvenes, debido a la variedad de causas posibles. En muchos casos, las consecuencias pueden ser potencialmente graves y conllevar importante morbi-mortalidad. La orientación diagnóstica precoz y un manejo adecuado son de gran importancia para minimizar las posibles secuelas y mejorar el pronóstico general del paciente

GMP-Compliant Manufacturing of NKG2D CAR Memory T Cells Using CliniMACS Prodigy

Natural killer group 2D (NKG2D) is a natural killer (NK) cell-activating receptor that recognizes different stress-induced ligands that are overexpressed in a variety of childhood and adult tumors. NKG2D chimeric antigen receptor (CAR) T cells have shown potent anticancer effects against different cancer types. A second-generation NKG2D CAR was generated by fusing full-length human NKG2D to 4-1BB costimulatory molecule and CD3ζ signaling domain. Patient-derived CAR T cells show limitations including inability to manufacture CAR T cells from the patients' own T cells, disease progression, and death prior to return of engineered cells. The use of allogeneic T cells for CAR therapy could be an attractive alternative, although undesirable graft vs. host reactions may occur. To avoid such adverse effects, we used CD45RA- memory T cells, a T-cell subset with less alloreactivity, as effector cells to express NKG2D CAR. In this study, we developed a protocol to obtain large-scale NKG2D CAR memory T cells for clinical use by using CliniMACS Prodigy, an automated closed system compliant with Good Manufacturing Practice (GMP) guidelines. CD45RA+ fraction was depleted from healthy donors' non-mobilized apheresis using CliniMACS CD45RA Reagent and CliniMACS Plus device. A total of 108 CD45RA- cells were cultured in TexMACS media supplemented with 100 IU/mL IL-2 and activated at day 0 with T Cell TransAct. Then, we used NKG2D-CD8TM-4-1BB-CD3ζ lentiviral vector for cell transduction (MOI = 2). NKG2D CAR T cells expanded between 10 and 13 days. Final cell products were analyzed to comply with the specifications derived from the quality and complementary controls carried out in accordance with the instructions of the Spanish Regulatory Agency of Medicines and Medical Devices (AEMPS) for the manufacture of investigational advanced therapy medicinal products (ATMPs). We performed four validations. The manufacturing protocol here described achieved large numbers of viable NKG2D CAR memory T cells with elevated levels of NKG2D CAR expression and highly cytotoxic against Jurkat and 531MII tumor target cells. CAR T cell final products met release criteria, except for one showing myc overexpression and another with viral copy number higher than five. Manufacturing of clinical-grade NKG2D CAR memory T cells using CliniMACS Prodigy is feasible and reproducible, widening clinical application of CAR T cell therapies.This study was funded in part by the National Health Service of Spain, Instituto de Salud Carlos III (ISCIII), FONDOS FEDER grant (FIS) PI18/01301, by the Unoentrecienmil Foundation and by CRIS Cancer Foundation to beat Cancer (http://criscancer.org).LF, AF, IM, and AE are granted by CRIS Cancer Foundation to beat cancer.S

Comprehensive description of clinical characteristics of a large systemic Lupus Erythematosus Cohort from the Spanish Rheumatology Society Lupus Registry (RELESSER) with emphasis on complete versus incomplete lupus differences

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by multiple organ involvement and pronounced racial and ethnic heterogeneity. The aims of the present work were (1) to describe the cumulative clinical characteristics of those patients included in the Spanish Rheumatology Society SLE Registry (RELESSER), focusing on the differences between patients who fulfilled the 1997 ACR-SLE criteria versus those with less than 4 criteria (hereafter designated as incomplete SLE (iSLE)) and (2) to compare SLE patient characteristics with those documented in other multicentric SLE registries. RELESSER is a multicenter hospital-based registry, with a collection of data from a large, representative sample of adult patients with SLE (1997 ACR criteria) seen at Spanish rheumatology departments. The registry includes demographic data, comprehensive descriptions of clinical manifestations, as well as information about disease activity and severity, cumulative damage, comorbidities, treatments and mortality, using variables with highly standardized definitions. A total of 4.024 SLE patients (91% with ≥4 ACR criteria) were included. Ninety percent were women with a mean age at diagnosis of 35.4 years and a median duration of disease of 11.0 years. As expected, most SLE manifestations were more frequent in SLE patients than in iSLE ones and every one of the ACR criteria was also associated with SLE condition; this was particularly true of malar rash, oral ulcers and renal disorder. The analysis-adjusted by gender, age at diagnosis, and disease duration-revealed that higher disease activity, damage and SLE severity index are associated with SLE [OR: 1.14; 95% CI: 1.08-1.20 (P < 0.001); 1.29; 95% CI: 1.15-1.44 (P < 0.001); and 2.10; 95% CI: 1.83-2.42 (P < 0.001), respectively]. These results support the hypothesis that iSLE behaves as a relative stable and mild disease. SLE patients from the RELESSER register do not appear to differ substantially from other Caucasian populations and although activity [median SELENA-SLEDA: 2 (IQ: 0-4)], damage [median SLICC/ACR/DI: 1 (IQ: 0-2)], and severity [median KATZ index: 2 (IQ: 1-3)] scores were low, 1 of every 4 deaths was due to SLE activity. RELESSER represents the largest European SLE registry established to date, providing comprehensive, reliable and updated information on SLE in the southern European population

Multicentre, randomised, single-blind, parallel group trial to compare the effectiveness of a Holter for Parkinson's symptoms against other clinical monitoring methods: study protocol

Introduction In recent years, multiple studies have aimed to develop and validate portable technological devices capable of monitoring the motor complications of Parkinson's disease patients (Parkinson's Holter). The effectiveness of these monitoring devices for improving clinical control is not known. Methods and analysis This is a single-blind, cluster-randomised controlled clinical trial. Neurologists from Spanish health centres will be randomly assigned to one of three study arms (1:1:1): (a) therapeutic adjustment using information from a Parkinson?s Holter that will be worn by their patients for 7 days, (b) therapeutic adjustment using information from a diary of motor fluctuations that will be completed by their patients for 7 days and (c) therapeutic adjustment using clinical information collected during consultation. It is expected that 162 consecutive patients will be included over a period of 6 months. The primary outcome is the efficiency of the Parkinson?s Holter compared with traditional clinical practice in terms of Off time reduction with respect to the baseline (recorded through a diary of motor fluctuations, which will be completed by all patients). As secondary outcomes, changes in variables related to other motor complications (dyskinesia and freezing of gait), quality of life, autonomy in activities of daily living, adherence to the monitoring system and number of doctor?patient contacts will be analysed. The noninferiority of the Parkinson's Holter against the diary of motor fluctuations in terms of Off time reduction will be studied as the exploratory objective. Ethics and dissemination approval for this study has been obtained from the Hospital Universitari de Bellvitge Ethics Committee. The results of this study will inform the practical utility of the objective information provided by a Parkinson's Holter and, therefore, the convenience of adopting this technology in clinical practice and in future clinical trials. We expect public dissemination of the results in 2022.Funding This work is supported by AbbVie S.L.U, the Instituto de Salud Carlos III [DTS17/00195] and the European Fund for Regional Development, 'A way to make Europe'

Immigrant IBD Patients in Spain Are Younger, Have More Extraintestinal Manifestations and Use More Biologics Than Native Patients

BackgroundPrevious studies comparing immigrant ethnic groups and native patients with IBD have yielded clinical and phenotypic differences. To date, no study has focused on the immigrant IBD population in Spain. MethodsProspective, observational, multicenter study comparing cohorts of IBD patients from ENEIDA-registry who were born outside Spain with a cohort of native patients. ResultsWe included 13,524 patients (1,864 immigrant and 11,660 native). The immigrants were younger (45 +/- 12 vs. 54 +/- 16 years, p < 0.001), had been diagnosed younger (31 +/- 12 vs. 36 +/- 15 years, p < 0.001), and had a shorter disease duration (14 +/- 7 vs. 18 +/- 8 years, p < 0.001) than native patients. Family history of IBD (9 vs. 14%, p < 0.001) and smoking (30 vs. 40%, p < 0.001) were more frequent among native patients. The most prevalent ethnic groups among immigrants were Caucasian (41.5%), followed by Latin American (30.8%), Arab (18.3%), and Asian (6.7%). Extraintestinal manifestations, mainly musculoskeletal affections, were more frequent in immigrants (19 vs. 11%, p < 0.001). Use of biologics, mainly anti-TNF, was greater in immigrants (36 vs. 29%, p < 0.001). The risk of having extraintestinal manifestations [OR: 2.23 (1.92-2.58, p < 0.001)] and using biologics [OR: 1.13 (1.0-1.26, p = 0.042)] was independently associated with immigrant status in the multivariate analyses. ConclusionsCompared with native-born patients, first-generation-immigrant IBD patients in Spain were younger at disease onset and showed an increased risk of having extraintestinal manifestations and using biologics. Our study suggests a featured phenotype of immigrant IBD patients in Spain, and constitutes a new landmark in the epidemiological characterization of immigrant IBD populations in Southern Europe