Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations

Maize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning. Of 14 B AFLPs, 13 were mapped by 12 B-10L translocations: 3 around the centromeric knob region, 3 in the proximal euchromatic, I around the border of proximal euchromatic and distal heterochromatic, and 6 in the distal heterochromatic region of the B long arm. The AFLP fragments were cloned and sequenced. Analogous to the microdissected sequences, all sequences were repetitive, and all but two were highly homologous to the A chromosomes. FISH signals of all but three clones appeared in pachytene B as well as in somatic A and B chromosomes. None of these clones exhibits identity to any published gene. Six clones displayed homology to two centromeric BACs, four to sequences of chromosomes 3, 4, 7, and 10, four to retrotransposons, and three to no sequence deposited in GenBank. Furthermore, flanking regions of two highly B-specific clones were characterized, showing extension of a B-exclusive nature. The possibility of the presence of novel B repeat(s) is discussed

Biharmonic Riemannian submersions from 3-manifolds

An important theorem about biharmonic submanifolds proved independently by Chen-Ishikawa [CI] and Jiang [Ji] states that an isometric immersion of a surface into 3-dimensional Euclidean space is biharmonic if and only if it is harmonic (i.e, minimal). In a later paper [CMO2], Cadeo-Monttaldo-Oniciuc shown that the theorem remains true if the target Euclidean space is replaced by a 3-dimensional hyperbolic space form. In this paper, we prove the dual results for Riemannian submersions, i.e., a Riemannian submersion from a 3-dimensional space form of non-positive curvature into a surface is biharmonic if and only if it is harmonic

Interference Cancellation Using Power Minimization and Self-coherence Properties of GPS Signals

This paper presents the performance analysis of two digital beam forming techniques used in conjunction with a software GPS receiver to mitigate interference to GPS signals in interference environment. The first method is the constrained minimum power (MOP) method. The second method is the so-called self-coherence restoral (SCORE) method. Both experimental and simulation data are used in the study. The study was performed using experiment data collected in an anechoic chamber to obtain GPS and interference signals. A two by two GPS antenna array and a four channel radio frequency front end were used to collect simulated GPS data generated using hardwarebased simulator in controlled interference environment. Three types of interference signals are deployed in the experiments: FM chirp, binary phase shift key, and broadband. The interference power levels used were +20, +30, and +40 dB above GPS signal power. A software GPS receiver was used to perform acquisition of GPS signals to evaluate the performance of the beam forming algorithms. The preliminary result showed that the MOP method can effectively mitigate all three types of interference at all power levels if a single interference source is present. Experiments using multiple broadband interference sources were also analyzed and our results shown that the effectiveness of the MOP method diminishes as the interference signal power increases and ceases to function at the +40 dB level. The SCORE method does not exhibit consistent performance for the experimental data. This is consistent with our simulation results which show that for the SCORE algorithm to generate satisfactory results, sufficient number of antenna elements is necessary even if there is no interference source present. The number of antenna element is determined by the number of satellites available, as well as the number of interference sources. The experimental and simulation results are discussed in this paper

CFD simulations of the spent fuel pool in the loss of coolant accident

Paper presented at the 9th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Malta, 16-18 July, 2012.The study utilized the computational fluid dynamics (CFD) methodology to investigate the thermal hydraulic behavior during the hypothetical event of normal operation and loss of cooling accident occurring at spent fuel pool. The boiling time, water level decreasing rate, fuel exposure time and temperature response after fuel exposure for the nuclear power plants under the accident were predicted in this study. We also analyze the flow and heat transfer for the single Atrium-10 fuel bundle. The details of the physics will be shown in this study. The results indicate that the fuel temperature in the pool will not exceed 1200°C to avoid the water-metal reaction after failure of RHR system for 4.578 days. We find that the velocity in the bundle are much faster than outside of the bundle under the LOCA accident.dc201

Wild tomato endosperm transcriptomes reveal common roles of genomic imprinting in both nuclear and cellular endosperm

Genomic imprinting is a conspicuous feature of the endosperm, a triploid tissue nurturing the embryo and synchronizing angiosperm seed development. An unknown subset of imprinted genes (IGs) is critical for successful seed development and should have highly conserved functions. Recent genome‐wide studies have found limited conservation of IGs among distantly related species, but there is a paucity of data from closely related lineages. Moreover, most studies focused on model plants with nuclear endosperm development, and comparisons with properties of IGs in cellular‐ type endosperm development are lacking. Using laser‐assisted microdissection, we characterized parent‐specific expression in the cellular endosperm of three wild tomato lineages (Solanum section Lycopersicon). We identified 1025 candidate IGs and 167 with putative homologs previously identified as imprinted in distantly related taxa with nuclear‐type endosperm. Forty‐two maternally expressed genes (MEGs) and 17 paternally expressed genes (PEGs) exhibited conserved imprinting status across all three lineages, but differences in power to assess imprinted expression imply that the actual degree of conservation might be higher than that directly estimated (20.7% for PEGs and 10.4% for MEGs). Regardless, the level of shared imprinting status was higher for PEGs than for MEGs, indicating dissimilar evolutionary trajectories. Expression‐level data suggest distinct epigenetic modulation of MEGs and PEGs, and gene ontology analyses revealed MEGs and PEGs to be enriched for different functions. Importantly, our data provide evidence that MEGs and PEGs interact in modulating both gene expression and the endosperm cell cycle, and uncovered conserved cellular functions of IGs uniting taxa with cellular‐ and nuclear‐type endosperm

An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types

Search for the standard model Higgs boson decaying to a bbˉb\bar{b} pair in events with no charged leptons and large missing transverse energy using the full CDF data set

We report on a search for the standard model Higgs boson produced in association with a vector boson in the full data set of proton-antiproton collisions at $\sqrt{s} = 1.96$ TeV recorded by the CDF II detector at the Tevatron, corresponding to an integrated luminosity of 9.45 fb$^{-1}$. We consider events having no identified charged lepton, a transverse energy imbalance, and two or three jets, of which at least one is consistent with originating from the decay of a $b$ quark. We place 95% credibility level upper limits on the production cross section times standard model branching fraction for several mass hypotheses between 90 and $150 \mathrm{GeV}/c^2$. For a Higgs boson mass of $125 \mathrm{GeV}/c^2$, the observed (expected) limit is 6.7 (3.6) times the standard model prediction.Comment: Accepted by Phys. Rev. Let

Search for the standard model Higgs boson decaying to a bb pair in events with one charged lepton and large missing transverse energy using the full CDF data set

We present a search for the standard model Higgs boson produced in association with a W boson in sqrt(s) = 1.96 TeV p-pbar collision data collected with the CDF II detector at the Tevatron corresponding to an integrated luminosity of 9.45 fb-1. In events consistent with the decay of the Higgs boson to a bottom-quark pair and the W boson to an electron or muon and a neutrino, we set 95% credibility level upper limits on the WH production cross section times the H->bb branching ratio as a function of Higgs boson mass. At a Higgs boson mass of 125 GeV/c2 we observe (expect) a limit of 4.9 (2.8) times the standard model value.Comment: Submitted to Phys. Rev. Lett (v2 contains clarifications suggested by PRL