56 research outputs found

    Fire Suppression Impacts on Fuels and Fire Intensity in the Western U.S.: Insights from Archaeological Luminescence Dating in Northern New Mexico

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    Here, we show that the last century of fire suppression in the western U.S. has resulted in fire intensities that are unique over more than 900 years of record in ponderosa pine forests (Pinus ponderosa). Specifically, we use the heat-sensitive luminescence signal of archaeological ceramics and tree-ring fire histories to show that a recent fire during mild weather conditions was more intense than anything experienced in centuries of frequent wildfires. We support this with a particularly robust set of optically stimulated luminescence measurements on pottery from an archaeological site in northern New Mexico. The heating effects of an October 2012 CE prescribed fire reset the luminescence signal in all 12 surface samples of archaeological ceramics, whereas none of the 10 samples exposed to at least 14 previous fires (1696–1893 CE) revealed any evidence of past thermal impact. This was true regardless of the fire behavior contexts of the 2012 CE samples (crown, surface, and smoldering fires). It suggests that the fuel characteristics from fire suppression at this site have no analog during the 550 years since the depopulation of this site or the 350 years of preceding occupation of the forested landscape of this region

    The role of tropical-extratropical interaction and synoptic variability in maintaining the South Pacific Convergence Zone in CMIP5 models

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    The South Pacific Convergence Zone (SPCZ) is simulated as too zonal a feature in current generation climate models, including those in Phase 5 of the Coupled Model Intercomparison Project (CMIP5). This zonal bias induces errors in tropical convective heating, with subsequent effects on global circulation. The SPCZ structure, particularly in the subtropics, is governed by the tropical-extratropical interaction between transient synoptic systems and the mean background state. However, the fidelity of synoptic-scale interactions as simulated by CMIP5 models has not yet been evaluated. In this study, analysis of synoptic variability in the simulated subtropical SPCZ reveals that the basic mechanism of tropical-extratropical interaction is generally well simulated, with storms approaching the SPCZ along comparable trajectories to observations. However, there is a broad spread in mean precipitation and its variability across the CMIP5 ensemble. Inter-model spread appears to relate to a biased background state in which the synoptic waves propagate. In particular, the region of mean negative zonal stretching deformation or "storm graveyard" in the upper troposphere?a feature previously determined to play a key role in SPCZ-storm interactions?is typically displaced in CMIP5 models to the northeast of its position in reanalysis data, albeit with individual model graveyards displaying a pronounced (25 degree) longitudinal spread. From these findings, we suggest that SPCZs simulated by CMIP5 models are not simply too zonal; rather, in models the subtropical SPCZ manifests a diagonal tilt similar to observations while SST biases force an overly zonal tropical SPCZ, resulting in a more disjointed SPCZ than observed

    SLC4A10 mutation causes a neurological disorder associated with impaired GABAergic transmission

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    SLC4A10 is a plasma-membrane bound transporter which utilizes the Na+ gradient to drive cellular HCO3- uptake, thus mediating acid extrusion. In the mammalian brain, SLC4A10 is expressed in principal neurons and interneurons, as well as in epithelial cells of the choroid plexus, the organ regulating the production of cerebrospinal fluid. Using next generation sequencing on samples from five unrelated families encompassing ten affected individuals, we show that biallelic SLC4A10 loss-of-function variants cause a clinically recognizable neurodevelopmental disorder in humans. The cardinal clinical features of the condition include hypotonia in infancy, delayed psychomotor development across all domains and typically severe intellectual impairment. Affected individuals commonly display traits associated with autistic spectrum disorders including anxiety, hyperactivity and stereotyped movements. In two cases isolated episodes of seizures were reported in the first few years of life, and a further affected child displayed bitemporal epileptogenic discharges on EEG without overt clinical seizures. While occipitofrontal circumference was reported to be normal at birth, progressive postnatal microcephaly evolved in 7 out of 10 affected individuals. Neuroradiological features included a relative preservation of brain volume compared to occipitofrontal circumference, characteristic narrow sometimes 'slit-like' lateral ventricles and corpus callosum abnormalities. Slc4a10 -/- mice, deficient for SLC4A10, also display small lateral brain ventricles and mild behavioral abnormalities including delayed habituation and alterations in the 2-object novel object recognition task. Collapsed brain ventricles in both Slc4a10-/- mice and affected individuals suggests an important role of SLC4A10 in the production of the cerebrospinal fluid. However, it is notable that despite diverse roles of the cerebrospinal fluid in the developing and adult brain, the cortex of Slc4a10-/- mice appears grossly intact. Co-staining with synaptic markers revealed that in neurons, SLC4A10 localizes to inhibitory, but not excitatory, presynapses. These findings are supported by our functional studies which show the release of the inhibitory neurotransmitter GABA is compromised in Slc4a10-/- mice, while the release of the excitatory neurotransmitter glutamate is preserved. Manipulation of intracellular pH partially rescues GABA release. Together our studies define a novel characteristic neurodevelopmental disorder associated with biallelic pathogenic variants in SLC4A10 and highlight the importance of further analyses of the consequences of SLC4A10 loss-of-function for brain development, synaptic transmission and network properties

    Probing the surface chemistry of self-assembled peptide hydrogels using solution-state NMR spectroscopy

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    The surface chemistry of self-assembled hydrogel fibres – their charge, hydrophobicity and ion-binding dynamics – is recognised to play an important role in determining how the gels develop as well as their suitability for different applications. However, to date there are no established methodologies for the study of this surface chemistry. Here, we demonstrate how solution-state NMR spectroscopy can be employed to measure the surface chemical properties of the fibres in a range of hydrogels formed from N-functionalised dipeptides, an effective and versatile class of gelator that has attracted much attention. By studying the interactions with the gel fibres of a diverse range of probe molecules and ions, we can simultaneously study a number of surface chemical properties of the NMR invisible fibres in an essentially non-invasive manner. Our results yield fresh insights into the materials. Most notably, gel fibres assembled using different tiggering methods bear differing amounts of negative charge as a result of a partial deprotonation of the carboxylic acid groups of the gelators. We also demonstrate how chemical shift imaging (CSI) techniques can be applied to follow the formation of hydrogels along chemical gradients. We apply CSI to study the binding of Ca2+ and subsequent gelation of peptide assemblies at alkaline pH. Using metal ion-binding molecules as probes, we are able to detect the presence of bound Ca2+ ions on the surface of the gel fibres. We briefly explore how knowledge of the surface chemical properties of hydrogels could be used to inform their practical application in fields such as drug delivery and environmental remediation
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