Texture is encoded in precise temporal spiking patterns in primate somatosensory cortex

Humans are exquisitely sensitive to the microstructure and material properties of surfaces. In the peripheral nerves, texture information is conveyed via two mechanisms: coarse textural features are encoded in spatial patterns of activation that reflect their spatial layout, and fine features are encoded in highly repeatable, texture-specific temporal spiking patterns evoked as the skin moves across the surface. Here, we examined whether this temporal code is preserved in the responses of neurons in somatosensory cortex. We scanned a diverse set of everyday textures across the fingertip of awake macaques while recording the responses evoked in individual cortical neurons. We found that temporal spiking patterns are highly repeatable across multiple presentations of the same texture, with millisecond precision. As a result, texture identity can be reliably decoded from the temporal patterns themselves, even after information carried in the spike rates is eliminated. However, the combination of rate and timing is more informative than either code in isolation. The temporal precision of the texture response is heterogenous across cortical neurons and depends on the submodality composition of their input and on their location along the somatosensory neuraxis. Furthermore, temporal spiking patterns in cortex dilate and contract with decreases and increases in scanning speed, respectively, and this systematic relationship between speed and patterning may contribute to the observed perceptual invariance to speed. Finally, we find that the quality of a texture percept can be better predicted when these temporal patterns are taken into consideration. We conclude that high-precision spike timing complements rate-based signals to encode texture in somatosensory cortex

Front-to-Rear Membrane Tension Gradient in Rapidly Moving Cells

AbstractMembrane tension is becoming recognized as an important mechanical regulator of motile cell behavior. Although membrane-tension measurements have been performed in various cell types, the tension distribution along the plasma membrane of motile cells has been largely unexplored. Here, we present an experimental study of the distribution of tension in the plasma membrane of rapidly moving fish epithelial keratocytes. We find that during steady movement the apparent membrane tension is ∼30% higher at the leading edge than at the trailing edge. Similar tension differences between the front and the rear of the cell are found in keratocyte fragments that lack a cell body. This front-to-rear tension variation likely reflects a tension gradient developed in the plasma membrane along the direction of movement due to viscous friction between the membrane and the cytoskeleton-attached protein anchors embedded in the membrane matrix. Theoretical modeling allows us to estimate the area density of these membrane anchors. Overall, our results indicate that even though membrane tension equilibrates rapidly and mechanically couples local boundary dynamics over cellular scales, steady-state variations in tension can exist in the plasma membranes of moving cells

A Two-Layer Model Explains Higher-Order Feature Selectivity of V2 Neurons

Neurons in cortical area V2 respond selectively to higher-order visual features, such as the quasi-periodic structure of natural texture. However, a functional account of how V2 neurons build selectivity for complex natural image features from their inputs – V1 neurons locally tuned for orientation and spatial frequency – remains elusive. We made single-unit recordings in area V2 in two fixating rhesus macaques. We presented stimuli composed of multiple superimposed grating patches that localize contrast energy in space, orientation, and scale. V2 activity is modeled via a two-layer linear-nonlinear network, optimized to use a sparse combination of V1-like outputs to account for observed activity. Analysis of model fits reveals V2 neurons to be well-matched to natural images, with units combining V1 afferent tuning dimensions to effectively capture natural scene variation. Remarkably, although the models are trained on responses to synthetic stimuli, they can predict responses to novel image classes, i.e. naturalistic texture, reproducing single-unit selectivity for higher-order image statistics. Thus, we demonstrate state-of-the art performance of modeling V2 selectivity, and provide a mechanistic account of single-unit tuning for higher-order natural features

Exiles and Migrants in Oceania

Humanities Open Book Program, a joint initiative of the National Endowment for the Humanities and the Andrew W. Mellon FoundationThe cultural and social consequences of uprooting island populations are the principal concerns of the anthropologists contributing to this first comparative study of resettled communities. The ten communities chosen for study include migrant groups like the Rotumans in Fiji as well as relocated ones like the people of Bikini Atoll or the Tikopia in the Russell Islands

Gate Coupling to Nanoscale Electronics

The realization of single-molecule electronic devices, in which a nanometer-scale molecule is connected to macroscopic leads, requires the reproducible production of highly ordered nanoscale gaps in which a molecule of interest is electrostatically coupled to nearby gate electrodes. Understanding how the molecule-gate coupling depends on key parameters is crucial for the development of high-performance devices. Here we directly address this, presenting two- and three-dimensional finite-element electrostatic simulations of the electrode geometries formed using emerging fabrication techniques. We quantify the gate coupling intrinsic to these devices, exploring the roles of parameters believed to be relevant to such devices. These include the thickness and nature of the dielectric used, and the gate screening due to different device geometries. On the single-molecule (~1nm) scale, we find that device geometry plays a greater role in the gate coupling than the dielectric constant or the thickness of the insulator. Compared to the typical uniform nanogap electrode geometry envisioned, we find that non-uniform tapered electrodes yield a significant three orders of magnitude improvement in gate coupling. We also find that in the tapered geometry the polarizability of a molecular channel works to enhance the gate coupling

Observation of metastable Aβ amyloid protofibrils by atomic force microscopy

AbstractBackground: Brain amyloid plaque, a diagnostic feature of Alzheimer's disease (AD), contains an insoluble fibrillar core that is composed primarily of variants of the β-amyloid protein (Aβ). As Aβ amyloid fibrils may initiate neurodegeneration, the inhibition of fibril formation is a possible therapeutic strategy. Very little is known about the early steps of the process, however.Results: Atomic force microscopy was used to follow amyloid fibril formation in vitro by the Aβ variants Aβ1-40 and Aβ1-42. Both variants first form small ordered aggregates that grow slowly and then rapidly disappear, while prototypical amyloid fibrils of two discrete morphologies appear. Aβ1-42 aggregates much more rapidly than Aβ1-40, which is consistent with its connection to early-onset AD. We propose that the metastable intermediate species be called Aβ amyloid protofibrils.Conclusions: Aβ protofibrils are likely to be intermediates in the in vitro assembly of Aβ amyloid fibrils, but their in vivo role has yet to be determined. Numerous reports of a nonfibrillar form of Aβ aggregate in the brains of individuals who are predisposed to AD suggest the existence of a precursor form, possibly the protofibril. Thus, stabilization of Aβ protofibrils may be a useful therapeutic strategy

Algorithm for Adapting Cases Represented in a Tractable Description Logic

Case-based reasoning (CBR) based on description logics (DLs) has gained a lot of attention lately. Adaptation is a basic task in the CBR inference that can be modeled as the knowledge base revision problem and solved in propositional logic. However, in DLs, it is still a challenge problem since existing revision operators only work well for strictly restricted DLs of the \emph{DL-Lite} family, and it is difficult to design a revision algorithm which is syntax-independent and fine-grained. In this paper, we present a new method for adaptation based on the DL $\mathcal{EL_{\bot}}$. Following the idea of adaptation as revision, we firstly extend the logical basis for describing cases from propositional logic to the DL $\mathcal{EL_{\bot}}$, and present a formalism for adaptation based on $\mathcal{EL_{\bot}}$. Then we present an adaptation algorithm for this formalism and demonstrate that our algorithm is syntax-independent and fine-grained. Our work provides a logical basis for adaptation in CBR systems where cases and domain knowledge are described by the tractable DL $\mathcal{EL_{\bot}}$.Comment: 21 pages. ICCBR 201

Structure of Flux Line Lattices with Weak Disorder at Large Length Scales

Dislocation-free decoration images containing up to 80,000 vortices have been obtained on high quality Bi$_{2}$Sr$_{2}$CaCu$_{2}$O$_{8+x}$ superconducting single crystals. The observed flux line lattices are in the random manifold regime with a roughening exponent of 0.44 for length scales up to 80-100 lattice constants. At larger length scales, the data exhibit nonequilibrium features that persist for different cooling rates and field histories.Comment: 4 pages, 3 gif images, to appear in PRB rapid communicatio

Relationship between the extent of non-viable myocardium and regional left ventricular function in chronic ischemic heart disease

Purpose. To define the relationship between left ventricular (LV) regional contractile function and the extent of myocardial scar in patients with chronic ischemic heart disease and multi-vessel coronary artery disease. Methods. Twenty-three patients with chronic ischemic heart disease and 5 healthy volunteers underwent magnetic resonance imaging (MRI). In patients, the relative area ( Percent Scar) and transmural extent (Transmurality) of myocardial infarction were computed from short-axis delayed enhancement images. In each image, myocardial segments were categorized based on the extent of infarction they contained, with 6 categories each for Percent Scar and Transmurality: normal, from healthy volunteers; and 0%; 1–25%, 26–50%, 51–75%, and \u3e 76% from patients. In patients and volunteers, regional LV function was quantified by absolute systolic wall thickening from cine images and midwall circumferential strain using tagged images. Results. Compared to normal segments, regional LV function in patients was significantly diminished in all scar extent intervals, with wall thickening=-8% for all categories. Systolic wall thickening was reduced significantly in all categories above 50% Percent Scar and above 25% Transmurality in patients, relative to corresponding 0% categories. Circumferential strain was significantly reduced above 25% Percent Scar and above 25% Transmurality. Conclusions. In patients with chronic ischemic heart disease and multivessel coronary artery disease, wall thickening was more sensitive to changes in scar Transmurality than to changes in Percent Scar. However, circumferential strain was equally sensitive to both indices. In general, circumferential strain was more sensitive than wall thickening to increases in scar extent

Relationship between the extent of non-viable myocardium and regional left ventricular function in chronic ischemic heart disease

Purpose. To define the relationship between left ventricular (LV) regional contractile function and the extent of myocardial scar in patients with chronic ischemic heart disease and multi-vessel coronary artery disease. Methods. Twenty-three patients with chronic ischemic heart disease and 5 healthy volunteers underwent magnetic resonance imaging (MRI). In patients, the relative area ( Percent Scar) and transmural extent (Transmurality) of myocardial infarction were computed from short-axis delayed enhancement images. In each image, myocardial segments were categorized based on the extent of infarction they contained, with 6 categories each for Percent Scar and Transmurality: normal, from healthy volunteers; and 0%; 1–25%, 26–50%, 51–75%, and \u3e 76% from patients. In patients and volunteers, regional LV function was quantified by absolute systolic wall thickening from cine images and midwall circumferential strain using tagged images. Results. Compared to normal segments, regional LV function in patients was significantly diminished in all scar extent intervals, with wall thickening=-8% for all categories. Systolic wall thickening was reduced significantly in all categories above 50% Percent Scar and above 25% Transmurality in patients, relative to corresponding 0% categories. Circumferential strain was significantly reduced above 25% Percent Scar and above 25% Transmurality. Conclusions. In patients with chronic ischemic heart disease and multivessel coronary artery disease, wall thickening was more sensitive to changes in scar Transmurality than to changes in Percent Scar. However, circumferential strain was equally sensitive to both indices. In general, circumferential strain was more sensitive than wall thickening to increases in scar extent