492 research outputs found
The Trypanosome Rab-Related Proteins RabX1 and RabX2 Play No Role in IntraCellular Trafficking but May Be Involved in Fly Infectivity
BACKGROUND: Rab GTPases constitute the largest subgroup of the Ras superfamily and are primarily involved in vesicle targeting. The full extent of Rab family function is unexplored. Several divergent Rab-like proteins are known but few have been characterized. In Trypanosoma brucei there are sixteen Rab genes, but RabX1, RabX2 and RabX3 are divergent within canonical sequence regions. Where known, trypanosome Rab functions are broadly conserved when orthologous relationships may be robustly established, but specific functions for RabX1, X2 and X3 have yet to be determined. RabX1 and RabX2 originated via tandem duplication and subcellular localization places RabX1 at the endoplasmic reticulum, while RabX2 is at the Golgi complex, suggesting distinct functions. We wished to determine whether RabX1 and RabX2 are involved in vesicle transport or other cellular processes. METHODOLOGY/PRINCIPAL FINDINGS: Using comparative genomics we find that RabX1 and RabX2 are restricted to trypanosomatids. Gene knockout indicates that RabX1 and RabX2 are non-essential. Simultaneous RNAi knockdown of both RabX1 and RabX2, while partial, was also non-lethal and may suggest non-redundant function, consistent with the distinct locations of the proteins. Analysis of the knockout cell lines unexpectedly failed to uncover a defect in exocytosis, endocytosis or in the morphology or location of multiple markers for the endomembrane system, suggesting that neither RabX1 nor RabX2 has a major role in intracellular transport. However, it was apparent that RabX1 and RabX2 knockout cells displayed somewhat enhanced survival within flies. CONCLUSIONS/SIGNIFICANCE: RabX1 and RabX2, two members of the trypanosome Rab subfamily, were shown to have no major detectable role in intracellular transport, despite the localization of each gene product to highly specific endomembrane compartments. These data extend the functional scope of Rab proteins in trypanosomes to include non-canonical roles in differentiation-associated processes in protozoa
SU(N) Quantum Antiferromagnets and the Phase Structure of QED in the Strong Coupling Limit
We examine the strong coupling limit of both compact and non compact QED on a
lattice with staggered fermions. We show that every SU(N) antiferromagnet with
spins in a particular fundamental representation of the SU(N) Lie Algebra and
with nearest neighbor couplings on a bipartite lattice is exactly equivalent to
the infinite coupling limit of lattice QED with the numbers of flavors of
electrons related to N and the dimension of spacetime D+1. We find that,for
both compact and noncompact QED,when N is odd the ground state of the strong
coupling limit breaks chiral symmetry in any dimensions and for any N and the
condensate is an isoscalar mass operator. When N is even,chiral symmetry is
broken if D is bigger or equal to 2 and N is small enough and the order
parameter is an isovector mass operator. We also find the exact ground state of
the lattice Coulomb gas as well as a variety of related lattice statistical
systems with long ranged interactions.Comment: latex, 45 pages, DFUPG 69/9
Tight-binding g-Factor Calculations of CdSe Nanostructures
The Lande g-factors for CdSe quantum dots and rods are investigated within
the framework of the semiempirical tight-binding method. We describe methods
for treating both the n-doped and neutral nanostructures, and then apply these
to a selection of nanocrystals of variable size and shape, focusing on
approximately spherical dots and rods of differing aspect ratio. For the
negatively charged n-doped systems, we observe that the g-factors for
near-spherical CdSe dots are approximately independent of size, but show strong
shape dependence as one axis of the quantum dot is extended to form rod-like
structures. In particular, there is a discontinuity in the magnitude of
g-factor and a transition from anisotropic to isotropic g-factor tensor at
aspect ratio ~1.3. For the neutral systems, we analyze the electron g-factor of
both the conduction and valence band electrons. We find that the behavior of
the electron g-factor in the neutral nanocrystals is generally similar to that
in the n-doped case, showing the same strong shape dependence and discontinuity
in magnitude and anisotropy. In smaller systems the g-factor value is dependent
on the details of the surface model. Comparison with recent measurements of
g-factors for CdSe nanocrystals suggests that the shape dependent transition
may be responsible for the observations of anomalous numbers of g-factors at
certain nanocrystal sizes.Comment: 15 pages, 6 figures. Fixed typos to match published versio
Large eddy simulation of a turbulent non-premixed propane-air reacting flame in a cylindrical combustor
Large Eddy Simulation (LES) is applied to investigate the turbulent non-premixed combustion flow, including species concentrations and temperature, in a cylindrical combustor. Gaseous propane (C3H8) is injected through a circular nozzle which is attached at the centre of the combustor inlet. Preheated air with a temperature of 773 K is supplied through the annulus surrounding of this fuel nozzle. In LES a spatial filtering is applied to the governing equations to separate the flow field into large-scale and small-scale eddies. The large-scale eddies which carry most of the turbulent energy are resolved explicitly, while the unresolved small-scale eddies are modelled using the Smagorinsky model with Cs = 0.1 as well as dynamically calibrated Cs. The filtered values of the species mass fraction, temperature and density, which are the functions of the mixture fraction (conserved scalar), are determined by integration over a beta probability density function (β-PDF). The computational results are compared with those of the experimental investigation conducted by Nishida and Mukohara. According to this experiment, the overall equivalence ratio of 0.6, which is calculated from the ratio of the air flow rate supplied to the combustion chamber to that of the stoichiometric reaction, is kept constant so that the turbulent combustion at the fuel nozzle exit starts under the fuel-rich conditions
Evolution of avalanche conducting states in electrorheological liquids
Charge transport in electrorheological fluids is studied experimentally under
strongly nonequlibrium conditions. By injecting an electrical current into a
suspension of conducting nanoparticles we are able to initiate a process of
self-organization which leads, in certain cases, to formation of a stable
pattern which consists of continuous conducting chains of particles. The
evolution of the dissipative state in such system is a complex process. It
starts as an avalanche process characterized by nucleation, growth, and thermal
destruction of such dissipative elements as continuous conducting chains of
particles as well as electroconvective vortices. A power-law distribution of
avalanche sizes and durations, observed at this stage of the evolution,
indicates that the system is in a self-organized critical state. A sharp
transition into an avalanche-free state with a stable pattern of conducting
chains is observed when the power dissipated in the fluid reaches its maximum.
We propose a simple evolution model which obeys the maximum power condition and
also shows a power-law distribution of the avalanche sizes.Comment: 15 pages, 6 figure
Optical properties of the pseudogap state in underdoped cuprates
Recent optical measurements of deeply underdoped cuprates have revealed that
a coherent Drude response persists well below the end of the superconducting
dome. In addition, no large increase in optical effective mass has been
observed, even at dopings as low as 1%. We show that this behavior is
consistent with the resonating valence bond spin-liquid model proposed by Yang,
Rice, and Zhang. In this model, the overall reduction in optical conductivity
in the approach to the Mott insulating state is caused not by an increase in
effective mass, but by a Gutzwiller factor, which describes decreased coherence
due to correlations, and by a shrinking of the Fermi surface, which decreases
the number of available charge carriers. We also show that in this model, the
pseudogap does not modify the low-temperature, low-frequency behavior, though
the magnitude of the conductivity is greatly reduced by the Gutzwiller factor.
Similarly, the profile of the temperature dependence of the microwave
conductivity is largely unchanged in shape, but the Gutzwiller factor is
essential in understanding the observed difference in magnitude between ortho-I
and -II YBaCuO.Comment: 9 pages, 6 figures, submitted to Eur. Phys. J.
Chiral Dynamics and Fermion Mass Generation in Three Dimensional Gauge Theory
We examine the possibility of fermion mass generation in 2+1- dimensional
gauge theory from the current algebra point of view.In our approach the
critical behavior is governed by the fluctuations of pions which are the
Goldstone bosons for chiral symmetry breaking. Our analysis supports the
existence of an upper critical number of Fermion flavors and exhibits the
explicit form of the gap equation as well as the form of the critical exponent
for the inverse correlation lenght of the order parameterComment: Latex,10 pages,DFUPG 70/9
Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water
The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 mu L L-1) and essential oil of Lippia alba (EOL) (10 or 20 mu L L-1) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L-1). The addition of various concentrations of EUG (1.5 or 3.0 mu L L-1) and EOL (10 or 20 mu L L-1) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 mu L L-1) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS/PRONEX) [10/0016-8]; Conselho Nacional de Pesquisa e Desenvolvimento Cientifico (CNPq) [470964/2009-0]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); CNPqinfo:eu-repo/semantics/publishedVersio
Measurement of Cosmic-ray Muon-induced Spallation Neutrons in the Aberdeen Tunnel Underground Laboratory
AbstractMuon-induced neutrons are one of the major backgrounds to various underground experiments, such as dark matter searches, low-energy neutrino oscillation experiments and neutrino-less double beta-decay experiments. Previous experiments on the underground production rate of muon-induced neutrons were mostly carried out either at shallow sites or at very deep sites. The Aberdeen Tunnel experiment aims to measure the neutron production rate at a moderate depth of 611 meters water equivalent. Our apparatus comprises of six layers of plastic-scintillator hodoscopes for tracking the incident cosmic-ray muons, and 760 L of gadolinium-doped liquid-scintillator for both neutron production and detection targets. In this paper, we describe the design and the performance of the apparatus. The preliminary result on the measurement of neutron production rate is also presented
Extrinsic CPT Violation in Neutrino Oscillations in Matter
We investigate matter-induced (or extrinsic) CPT violation effects in
neutrino oscillations in matter. Especially, we present approximate analytical
formulas for the CPT-violating probability differences for three flavor
neutrino oscillations in matter with an arbitrary matter density profile. Note
that we assume that the CPT invariance theorem holds, which means that the CPT
violation effects arise entirely because of the presence of matter. As special
cases of matter density profiles, we consider constant and step-function matter
density profiles, which are relevant for neutrino oscillation physics in
accelerator and reactor long baseline experiments as well as neutrino
factories. Finally, the implications of extrinsic CPT violation on neutrino
oscillations in matter for several past, present, and future long baseline
experiments are estimated.Comment: 47 pages, 7 figures, RevTeX4. Final version to be published in Phys.
Rev.
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