919 research outputs found
Comparison of the membrane binding kinetics of bovine prothrombin and its fragment 1
Total internal reflection fluorescence microscopy has been used to compare the membrane binding characteristics of fluorescein-labeled bovine prothrombin and fluorescein-labeled bovine prothrombin fragment 1. The Ca(2+)-dependent association of these proteins with quartz-supported planar membranes composed of mixtures of phosphatidylserine (2-10 mol%) and phosphatidylcholine was examined. Equilibrium binding measurements showed that the apparent equilibrium dissociation constants increased with decreasing molar fractions of phosphatidylserine and that the dissociation constants were somewhat lower for intact prothrombin. Kinetic measurements, using fluorescence photobleaching recovery, showed that the measured dissociation rates were approximately equivalent for prothrombin and fragment 1 and did not change with the protein solution concentration or the molar fraction of phosphatidylserine. The kinetic data also implied that the surface binding mechanism for both proteins is more complex than a simple reversible reaction between monovalent proteins and monovalent surface sites. Measured equilibrium and kinetic constants are reported and compared for prothrombin and fragment 1 on planar membranes
Quantitative analysis of cell types during growth and morphogenesis in Hydra
Tissue maceration was used to determine the absolute number and the distribution of cell types in Hydra. It was shown that the total number of cells per animal as well as the distribution of cells vary depending on temperature, feeding conditions, and state of growth. During head and foot regeneration and during budding the first detectable change in the cell distribution is an increase in the number of nerve cells at the site of morphogenesis. These results and the finding that nerve cells are most concentrated in the head region, diminishing in density down the body column, are discussed in relation to tissue polarity
Evidence for Asphericity in the Type IIn Supernova 1998S
We present optical spectropolarimetry obtained at the Keck-II 10-m telescope
on 1998 March 7 UT along with total flux spectra spanning the first 494 days
after discovery (1998 March 2 UT) of the peculiar type IIn supernova (SN)
1998S. The SN is found to exhibit a high degree of linear polarization,
implying significant asphericity for its continuum-scattering environment.
Prior to removal of the interstellar polarization, the polarization spectrum is
characterized by a flat continuum (at p ~ 2%) with distinct changes in
polarization associated with both the broad (FWZI >= 20,000 km/s) and narrow
(unresolved, FWHM < 300 km/s) line emission seen in the total flux spectrum.
When analyzed in terms of a polarized continuum with unpolarized broad-line
recombination emission, an intrinsic continuum polarization of p ~ 3% results
(the highest yet found for a SN), suggesting a global asphericity of >= 45%
from the oblate, electron-scattering dominated models of Hoflich (1991). The
smooth, blue continuum evident at early times is shown to be inconsistent with
a reddened, single-temperature blackbody, instead having a color temperature
that increases with decreasing wavelength. Broad emission-line profiles with
distinct blue and red peaks are seen in the total flux spectra at later times,
perhaps suggesting a disk-like or ring-like morphology for the dense (n_e ~
10^7 cm^{-3}) circumstellar medium. Implications of the circumstellar
scattering environment for the spectropolarimetry are discussed, as are the
effects of uncertain removal of interstellar polarization.Comment: 25 pages + 2 tables + 14 figures, Submitted to The Astrophysical
Journa
Gravitational Waves from Core Collapse Supernovae
We present the gravitational wave signatures for a suite of axisymmetric core
collapse supernova models with progenitors masses between 12 and 25 solar
masses. These models are distinguished by the fact they explode and contain
essential physics (in particular, multi-frequency neutrino transport and
general relativity) needed for a more realistic description. Thus, we are able
to compute complete waveforms (i.e., through explosion) based on
non-parameterized, first-principles models. This is essential if the waveform
amplitudes and time scales are to be computed more precisely. Fourier
decomposition shows that the gravitational wave signals we predict should be
observable by AdvLIGO across the range of progenitors considered here. The
fundamental limitation of these models is in their imposition of axisymmetry.
Further progress will require counterpart three-dimensional models.Comment: 10 pages, 5 figure
Influence of hyperhomocysteinemia on the cellular redox state - Impact on homocysteine-induced endothelial dysfunction
Hyperhomocysteinemia is an independent risk factor for the development of atherosclerosis. An increasing body of evidence has implicated oxidative stress as being contributory to homocysteines deleterious effects on the vasculature. Elevated levels of homocysteine may lead to increased generation of superoxide by a biochemical mechanism involving nitric oxide synthase, and, to a lesser extent, by an increase in the chemical oxidation of homocysteine and other aminothiols in the circulation. The resultant increase in superoxide levels is further amplified by homocysteinedependent alterations in the function of cellular antioxidant enzymes such as cellular glutathione peroxidase or extracellular superoxide dismutase. One direct clinical consequence of elevated vascular superoxide levels is the inactivation of the vasorelaxant messenger nitric oxide, leading to endothelial dysfunction. Scavenging of superoxide anion by either superoxide dismutase or 4,5-dihydroxybenzene 1,3-disulfonate (Tiron) reverses endothelial dysfunction in hyperhomocysteinemic animal models and in isolated aortic rings incubated with homocysteine. Similarly, homocysteineinduced endothelial dysfunction is also reversed by increasing the concentration of the endogenous antioxidant glutathione or overexpressing cellular glutathione peroxidase in animal models of mild hyperhomocysteinemia. Taken together, these findings strongly suggest that the adverse vascular effects of homocysteine are at least partly mediated by oxidative inactivation of nitric oxide
Spectra and Light Curves of Six Type Ia Supernovae at 0.511 < z < 1.12 and the Union2 Compilation
We report on work to increase the number of well-measured Type Ia supernovae
(SNe Ia) at high redshifts. Light curves, including high signal-to-noise HST
data, and spectra of six SNe Ia that were discovered during 2001 are presented.
Additionally, for the two SNe with z>1, we present ground-based J-band
photometry from Gemini and the VLT. These are among the most distant SNe Ia for
which ground based near-IR observations have been obtained. We add these six
SNe Ia together with other data sets that have recently become available in the
literature to the Union compilation (Kowalski et al. 2008). We have made a
number of refinements to the Union analysis chain, the most important ones
being the refitting of all light curves with the SALT2 fitter and an improved
handling of systematic errors. We call this new compilation, consisting of 557
supernovae, the Union2 compilation. The flat concordance LambdaCDM model
remains an excellent fit to the Union2 data with the best fit constant equation
of state parameter w=-0.997^{+0.050}_{-0.054} (stat) ^{+0.077}_{-0.082}
(stat+sys\ together) for a flat universe, or w=-1.035^{+0.055}_{-0.059}
(stat)^{+0.093}_{-0.097} (stat+sys together) with curvature. We also present
improved constraints on w(z). While no significant change in w with redshift is
detected, there is still considerable room for evolution in w. The strength of
the constraints depend strongly on redshift. In particular, at z > 1, the
existence and nature of dark energy are only weakly constrained by the data.Comment: 33 pages, 18 figures; accepted for publication in Astrophysical
Journal. For data tables, code for cosmological analysis and full-resolution
figures, see http://supernova.lbl.gov/Union
Supernova Legacy Survey: Using Spectral Signatures To Improve Type Ia Supernovae As Distance Indicators
GMOS optical long-slit spectroscopy at the Gemini-North telescope was used to
classify targets from the Supernova Legacy Survey (SNLS) from July 2005 and May
2006 - May 2008. During this time, 95 objects were observed. Where possible the
objects' redshifts (z) were measured from narrow emission or absorption
features in the host galaxy spectrum, otherwise they were measured from the
broader supernova features. We present spectra of 68 confirmed or probable SNe
Ia from SNLS with redshifts in the range 0.17 \leq z \leq 1.02. In combination
with earlier SNLS Gemini and VLT spectra, we used these new observations to
measure pseudo-equivalent widths (EWs) of three spectral features - CaII H&K,
SiII and MgII - in 144 objects and compared them to the EWs of low-redshift SNe
Ia from a sample drawn from the literature. No signs of changes with z are seen
for the CaII H&K and MgII features. Systematically lower EW SiII is seen at
high redshift, but this can be explained by a change in demographics of the SNe
Ia population within a two-component model combined with an observed
correlation between EW SiII and photometric lightcurve stretch.Comment: 49 pages including 2 online-only appendices, accepted for publication
in MNRA
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