Role of non-coding RNAs in non-aging-related neurological disorders

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOProtein coding sequences represent only 2% of the human genome. Recent advances have demonstrated that a significant portion of the genome is actively transcribed as non-coding RNA molecules. These non-coding RNAs are emerging as key players in the regula518CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOsem informaçãosem informação2016/22447-52011/506802013/07559-

Integrated utilization of grape skins from white grape pomaces

An approach for the integrated utilization of grape skins from white grape pomaces has been proposed. This consists of consecutive or simultaneous extraction of grape skins with neutral organic solvent and water under reflux. Organic extract is a valuable raw material for the isolation of oleanolic acid. The aqueous extract (ca 50%, w/w) is composed of essentially hexoses and suitable for the high yield (till 51%) bioethanol production at a maximum specific cell growth rate (μmax) of 0.29 h−1. The remained extracted grape skins are the complex of structural polysaccharides embedded into cutinous matrix. Extracted grape skins were shown to be a prospective raw material for the production of low-density boards (d ≤ 0.40) for insulation needs. The boards produced from grape skins and bind of 8% urea–formaldehyde resin revealed reasonable tensile strength (0.4 MPa) and a low thermal conductivity (0.09–0.12 W (m K)−1) over a wide range of temperatures (40–200 °C).info:eu-repo/semantics/publishedVersio

PCR identification of Mycobacterium tuberculosis complex in a clinical sample from a patient with symptoms of tuberculous spondylodiscitis

A 42-year-old male complaining of thoracic spine pain was admitted to the hospital for evaluation. An X-ray and computer tomography of the thoracic spine showed spondylodiscitis of the L3 lumbar and L2-L3 intervertebral disk. The tuberculin skin test (PPD) was strongly positive. A radioscopy-guided fine needle aspirate of the affected area was cultured but did not reveal the cause of the disease. Two biopsy attempts failed to reveal the cause of the disease by culturing or by acid-fast-resistant staining (Ziehl Neelsen) of the specimens. A third biopsy also failed to detect the infectious agent by using microbiological procedures, but revealed the presence of a 245-bp amplicon characteristic of the Mycobacterium tuberculosis complex after PCR of the sample. The result demonstrates the efficacy of PCR for the identification of M. tuberculosis in situations in which conventional diagnosis by culturing techniques or direct microscopy is unable to detect the microorganism. Following this result the patient was treated with the antituberculous cocktail composed by rifampicin, pirazinamide and isoniazid during a six-month period. At the end of the treatment the dorsalgia symptoms had disappeared

Locus Of Control And Contraceptive Knowledge, Attitude And Practice Among University Students [lócus De Controle E Conhecimento, Atitude E Prática Contraceptivas Entre Adolescentes Universitários]

Objective: To assess the relationship between locus of control and knowledge, attitude and practice regarding pill and condom use among university students. Methods: The inquiry was developed in Campinas, a city in Southeastern Brazil, in 2006. A total of 295 adolescent newcomers to a public university answered a structured questionnaire and Levenson's multidimensional locus of control scale. The scores of the dimensions of locus of control were calculated and Spearman's correlation coefficient was used to assess their correlation with knowledge and practice concerning pill and condom use. In order to assess the relationship between the dimensions of locus of control and sociodemographic variables and variables related to the individuals' sex life, Kruskal-Wallis and Mann-Whitney tests were used. RESULTS: Male adolescents had higher scores of powerful others externality when compared to female adolescents (p=0.01). Students living alone had lower internality (p=0.01). When locus of control was compared to condom use in the first intercourse, considering only the 102 students who informed the age of the beginning of sexual activity, greater internality was found among male adolescents who did not use condoms (p<0.05). When the locus of control scores were correlated with contraceptive knowledge and practice, it was found that the higher the powerful others externality locus, the lower the adequate use of contraceptive methods (r= -0.22, p=0.03). Conclusions: The powerful others externality locus influences the practice of contraceptive use in this group of adolescents.4413944Boruchovitch, E., Fatores associados a não-utilização de anticoncepcionais na adolescência (1992) Rev Saude Publica, 26 (6), pp. 437-443. , DOI:10.1590/S0034-89101992000600010Chubb, N.H., Fertman, C.I., Ross, J.L., Adolescent self-esteem and locus of control: a longitudinal study of gender and age differences (1997) Adolescence, 32 (125), pp. 113-129Commendador, K.A., Concept analysis of adolescent decision making and contraception (2003) Nurs Forum, 38 (4), pp. 27-35. , DOI:10.1111/j.0029-6473.2003.00027.xDela Coleta, M.F., Escala multidimensional de locus de controle de Levenson (1987) Arq Bras Psic, 39 (2), pp. 79-97Harvey, A.L., Risky and safe contraceptors: some personality factors (1976) J Psychol, 92 (1), pp. 109-112. , Half(1988) Designing clinical research: an epidemiological approach, p. 218. , Hulley, S.B., Cummings, S.R., editors. Baltimore: Williams & WilkinsMacDonald A.P., Jr., Internal-external locus of control and the practice of birth control (1970) Psychol Rep, 27 (1), p. 206Pirotta, K.C.M., Schor, N., Intenções reprodutivas e práticas de regulação da fecundidade entre universitários (2004) Rev Saude Publica, 38 (4), pp. 495-502. , DOI:10.1590/S0034-89102004000400003Ponto, M.T., Relationship between students' locus of control and satisfaction (1999) Br J Nurs, 8 (3), pp. 176-181Schor, N., Lopez, A.F., Adolescência e anticoncepção: 1. Estudo de conhecimento e uso em puérperas internadas por parto ou aborto (1990) Rev Saude Publica, 24 (6), pp. 506-511. , DOI:10.1590/S0034-89101990000600009Visher, S., The relationship of locus of control and contraception use in the adolescent population (1986) J Adolesc Health Care, 7 (3), pp. 183-186. , DOI:10.1016/S0197-0070(86)80036-5Young, T.M., Martin, S.S., Young, M.E., Ting, L., Internal poverty and teen pregnancy (2001) Adolescence, 36 (142), pp. 289-304Zanetti, M.L., Mendes, I.A., Tendência do locus de controle de pessoas diabéticas (1993) Rev Esc Enferm USP, 27 (2), pp. 246-26

Efeito da aplicação de diferentes regimes de rega deficitária no pessegueiro ‘Sweet Dream’ cultivado num pomar da região da Beira Interior.

Efeito da aplicação de diferentes regimes de rega deficitária no pessegueiro ‘Sweet Dream’ cultivado num pomar da região da Beira Interior.info:eu-repo/semantics/publishedVersio

Efeito da aplicação de diferentes regimes de rega deficitária no pessegueiro ‘Sweet Dream’ cultivado num pomar da região da Beira Interior.

Efeito da aplicação de diferentes regimes de rega deficitária no pessegueiro ‘Sweet Dream’ cultivado num pomar da região da Beira Interior.info:eu-repo/semantics/publishedVersio

Detection of Borrelia lusitaniae, Rickettsia sp. IRS3, Rickettsia monacensis and Anaplasma phagocytophilum in Ixodes ricinus collected in Madeira Island, Portugal

A total of 300 Ixodes ricinus ticks were tested by polymerase chain reaction (PCR) for the presence of Borrelia spp., Rickettsia spp., and Anaplasma phagocytophilum. Sequence analysis demonstrated 8 (2.7%) ticks infected with B. lusitaniae, 60 (20%) with Rickettsia spp., and 1 (0.3%) with A. phagocytophilum. Seven (2.3%) ticks were coinfected with B. lusitaniae and Rickettsia spp., 2 (0.6%) with R. monacensis, and 5 (1.7%) with Rickettsia sp. IRS3. The results of this study suggest simultaneous transmission of multiple tick-borne agents on Madeira Island, Portugal

Influência da desfolha na casta ‘Cabernet Sauvignon’

Num ensaio instalado em 2003 numa vinha da Quinta de Pancas em Alenquer, estudou-se a influência de três intensidades de desfolha (D0- não desfolhada, D1- desfolha do lado nascente e D2- desfolha dos dois lados da sebe) e duas épocas de realização (BE- bago de ervilha e PI- pintor) na ecofisiologia da videira, componentes da produção e qualidade do mosto na casta Cabernet Sauvignon. A desfolha alterou significativamente a estrutura do coberto, provocando a redução do número de camadas de folhas, um aumento da porosidade e da exposição dos cachos e, consequentemente, uma alteração significativa do seu microclima luminoso e térmico. As diferentes épocas de desfolha não provocaram diferenças significativas nos resultados. Apesar da desfolha não ter provocado alterações significativas na produção, ao nível das características do mosto à vindima, a modalidade desfolhada dos dois lados originou um aumento significativo nos fenóis totais e na intensidade da cor, comparativamente às restantes modalidadesinfo:eu-repo/semantics/publishedVersio

Operational research IO 2021—analytics for a better world. XXI Congress of APDIO, Figueira da Foz, Portugal, November 7–8, 2021

This book provides the current status of research on the application of OR methods to solve emerging and relevant operations management problems. Each chapter is a selected contribution of the IO2021 - XXI Congress of APDIO, the Portuguese Association of Operational Research, held in Figueira da Foz from 7 to 8 November 2021. Under the theme of analytics for a better world, the book presents interesting results and applications of OR cutting-edge methods and techniques to various real-world problems. Of particular importance are works applying nonlinear, multi-objective optimization, hybrid heuristics, multicriteria decision analysis, data envelopment analysis, simulation, clustering techniques and decision support systems, in different areas such as supply chain management, production planning and scheduling, logistics, energy, telecommunications, finance and health. All chapters were carefully reviewed by the members of the scientific program committee.info:eu-repo/semantics/publishedVersio

Development and method validation for determination of 128 pesticides in bananas by modified QuEChERS and UHPLC–MS/MS analysis

AbstractA multiresidue method for the quantification of 128 pesticides in banana is described. It involves the application of a modified QuEChERS procedure followed by UHPLC–MS/MS (Ultra High Performance Liquid Chromatography coupled to Tandem Mass Spectrometry) analysis. The method was validated according to the European Union SANCO/12495/2011 guidelines and Brazilian Manual of Analytical Quality Assurance. The validation levels were 10.0; 25.0; 50.0 and 100 μg kg−1. Acceptable values were obtained for the following parameters: linearity, limit of detection – LOD (5.00 μg kg−1) and limit of quantification – LOQ (10.0 μg kg−1), except for fenamiphos and mevinphos (LOD = 7.5 μg kg−1 and LOQ = 25 μg kg−1), trueness (for the levels: 10.0, 25.0, 50.0 and 100 μg kg−1 the recovery assays values were between 70 and 120%) except for methamidophos at 10 μg kg−1 level (67.5%), intermediate precision (<20.0%) and measurement uncertainty tests (<50.0%). These results demonstrate the applicability of this method in the routine practice by the laboratories of Ministry of Agriculture, Livestock and Food Supply of Brazil that attend the National Control Plan for Residues and Contaminants (PNCRC)