Anterior cruciate ligament reconstruction: A multicenter prospective cohort study evaluating 3 different grafts using same bone drilling method

To compare the clinical outcomes after anterior cruciate ligament (ACL) reconstruction with bone-patellar tendon-bone autograft (BPTBAu), BPTB allograft (BPTBAll), or hamstring (semitendinosus-gracilis) tendon autograft (HTAu), performing bone drilling with same methods in terms of transtibial drilling, orientation, positioning, and width of femoral and tibial tunnels. DESIGN: Multicenter prospective cohort study (level of evidence II). SETTING: Departments of Orthopedic Surgery of Centro Medico Teknon (Barcelona, Spain) Clinica Universitaria de Navarra (Navarra, Spain), and Clinica FREMAP (Gijon, Spain). PATIENTS: All patients with ACL tears attending 3 different institutions between January 2004 and June 2006 were approached for eligibility and those meeting inclusion criteria finally participated in this study. INTERVENTION: Each institution was assigned to perform a specific surgical technique. Patients were prospectively followed after undergoing ACL reconstruction with BPTBAu, BPTBAll, or HTAu, with a minimum follow-up of 24 months. MAIN OUTCOME MEASURES: Included knee laxity and International Knee Documentation Committee (IKDC) score. Knee laxity was assessed with the KT-1000 arthrometer (evaluated with neutral and external rotation positions) and both Lachman and pivot shift tests. Additional outcomes included main symptoms (anterior knee pain, swelling, crepitation, and instability), disturbance in knee sensation, visual analogue scale (VAS) for satisfaction with surgery, range of motion (ROM), and isokinetic knee strength. RESULTS: There were no significant differences among the 3 groups for any of the clinical outcomes, except for a slightly greater KT-1000-measured knee laxity in external rotation in the BPTBAu compared with the other groups. All patients demonstrated grade A or B of the IKDC. The mean VAS for satisfaction with surgery in all patients was 8.5. CONCLUSIONS: The selection of the surgical technique for ACL reconstruction may be based on the surgeon's preference

Limestone amendments as a measure against root rot of Quercus sp. caused by P. Cinnamomi in dehesa systems

Ponencia presentada a la 51 Reunión Científica de la SEEP celebrada en la Escuela Superior de Ingenieros Agrónomos de la Universidad Pública de Navarra entre el 14 y el 18 de mayo de 2012.La podredumbre radical causada por los patógenos de suelo Phytophthora cinnamomi y Pythium spiculum, está ocasionando una mortalidad elevada en el arbolado de algunas dehesas del suroeste de la península Iberica. Los experimentos llevados a cabo en condiciones controladas con suelo infectado artificialmente, han puesto de manifiesto que algunos compuestos cálcicos reducen la viabilidad de P. cinnamomi, principalmente debido a una disminución significativa de la producción de esporangios. En otoño del 2010 se puso en marcha un experimento de aplicación de enmiendas calizas con distintos productos en dehesas de la provincia de Huelva con podredumbre radical en su arbolado causada por P. cinnamomi, evaluándose los cambios en variables edáficas, la evolución del patógeno en suelo y de la defoliación de la copa del arbolado. Los resultados obtenidos hasta ahora indican que las enmiendas calizas podrían limitar la capacidad infectiva de Phytophthora cinnamomi en la dehesa, corroborando lo que se observa en condiciones controladas.The root rot caused by the soilborne pathogens Phytophthora cinnamomi and Pythium spiculum, is leading to significant oak tree mortality in dehesas farms in southwestern of Iberian Peninsula. Experiments performed with artificially infested soil in controlled conditions showed that some Ca chemicals induce a decrease of P. cinnamomi viability, mainly due to a significant inhibition of sporangial production. An experiment with different types of limestone amendments was carrying on during autumn of 2010 in dehesas farms with oak root rot caused by P. cinnamomi in Huelva province. We evaluated the change of soil parameters, the evolution of density of soil pathogens and defoliation of tree crown. So far, results obtained indicate that limestone amendments may limit the infectivity of P. cinnamomi, corroborating results observed under controlled conditions

Fe3O4-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy

In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model.Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86655-R and BIO2017-84246-C2-1-R)Fondo Social de la DGA (grupos DGA) and by the Regional Ministry of Health of Andalusia (OH-0026-2018).Associate Laboratory for Green Chemistry—LAQVApplied Molecular Biosciences Unit –UCIBIOPortuguese national funds from FCT/MCTES (UIDB/04378/2020 and UIDB/50006/2020

Influence of grazing intensity on leaf nutrients content of holm oak at dehesa

Ponencia presentada a la 51 Reunión Científica de la SEEP celebrada en la Escuela Superior de Ingenieros Agrónomos de la Universidad Pública de Navarra entre el 14 y el 18 de mayo de 2012.El presente trabajo trata de evaluar el efecto de la intensidad de pastoreo sobre el contenido en nutrientes foliares de la encina en la dehesa. Para ello se escogieron 14 parcelas de dehesa en Los Pedroches, en cada una de las cuales se tomaron muestras de hoja de siete encinas, evaluándose contenido en macronutrientes, muestras de suelo para determinaciones físicas y químicas y se midió la resistencia del suelo a la penetración. A partir de los datos de suelo se han clasificado las parcelas en dos grandes grupos, constituido uno por aquellas parcelas que habitualmente reciben mayor pastoreo y otro por aquellas que soportan una menor intensidad de pastoreo. Los resultados indican que el pastoreo afecta al contenido foliar de nutrientes en la encina, aumentando la concentración de N y Mg con la intensidad del pastoreo.This study assesses the effect of grazing intensity over nutrient contents of holm oak in dehesa system. Fourteen dehesa plots were chosen in Los Pedroches. In each plot, leaf and soil samples were collected and soil resistance to penetration was assessed. Results shows that plot classification according to grazing intensity can be achieved from soil data. Moreover, foliar nutrient contents in holm oak are affected by grazing intensity, increasing N and Mg foliar contents in plots heavily grazed

An imaging spectroscopic survey of the planetary nebula NGC 7009 with MUSE

Interstellar matter and star formatio