Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella

Plastic degradation by biological systems with re-utilization of the by-products could be a future solution to the global threat of plastic waste accumulation. Here, we report that the saliva of Galleria mellonella larvae (wax worms) is capable of oxidizing and depolymerizing polyethylene (PE), one of the most produced and sturdy polyolefin-derived plastics. This effect is achieved after a few hours’ exposure at room temperature under physiological conditions (neutral pH). The wax worm saliva can overcome the bottleneck step in PE biodegradation, namely the initial oxidation step. Within the saliva, we identify two enzymes, belonging to the phenol oxidase family, that can reproduce the same effect. To the best of our knowledge, these enzymes are the first animal enzymes with this capability, opening the way to potential solutions for plastic waste management through bio-recycling/up-cycling

The welfare of water buffaloes during the slaughter process: a review

This paper reviews the scientific literature on water buffalo welfare in all stages of the live animal supply chain from the farm gate to slaughter (loading/unloading, markets, transportation, handling, lairage, stunning and slaughter) with the objective of identifying risk factors and potential mitigation strategies. Although in some countries legislation exists to protect the welfare of farm animals during transport and killing, the handling practices used to load and unload buffaloes and move them in livestock markets and abattoirs are often harsh. This is frequently due to inadequate equipment designed principally for cattle, and the fact that water buffaloes are considered more temperamental than cattle. Additionally, more reactive animals have increased stress responses to handling, which can lead to more negative human interventions with increased numbers of skin lesions and bruises to the carcasses. During transport, buffaloes may suffer periods of thermal stress due to overstocking, inadequate ventilation and because in many tropical climates trips are made during the hottest time of the day. The anatomical and physiological characteristics of water buffalo make them particularly susceptible to thermal stress in the absence of water for wallowing. Although water buffaloes belong to the same Bovidae family as domestic cattle, certain anatomical features of the head make effective stunning very problematic. Buffaloes have extensive sinuses and frontal bones, meaning that the penetrating captive bolt devices recommended for cattle may prove ineffective in reliably inducing unconsciousness. There is a need for further development of procedures, stunning positions and appropriate devices to improve the efficiency of buffalo stunning. Finally, in many parts of the world where buffalo are routinely slaughtered in basic conditions without prior stunning. Slaughter without stunning can result in pain and stress associated with delays in the time to loss of consciousness, pain from the cutting of the neck and potential distress associated with aspiration of blood into the respiratory tract. Specific legislation, guidelines and handler/stockman/operator training programmes should be developed to improve the welfare of buffaloes during all ante mortem stages of loading, unloading, handling, stunning and slaughter

Centering inclusivity in the design of online conferences: An OHBM-Open Science perspective

As the global health crisis unfolded, many academic conferences moved online in 2020. This move has been hailed as a positive step towards inclusivity in its attenuation of economic, physical, and legal barriers and effectively enabled many individuals from groups that have traditionally been underrepresented to join and participate. A number of studies have outlined how moving online made it possible to gather a more global community and has increased opportunities for individuals with various constraints, e.g., caregiving responsibilities. Yet, the mere existence of online conferences is no guarantee that everyone can attend and participate meaningfully. In fact, many elements of an online conference are still significant barriers to truly diverse participation: the tools used can be inaccessible for some individuals; the scheduling choices can favour some geographical locations; the set-up of the conference can provide more visibility to well-established researchers and reduce opportunities for early-career researchers. While acknowledging the benefits of an online setting, especially for individuals who have traditionally been underrepresented or excluded, we recognize that fostering social justice requires inclusivity to actively be centered in every aspect of online conference design. Here, we draw from the literature and from our own experiences to identify practices that purposefully encourage a diverse community to attend, participate in, and lead online conferences. Reflecting on how to design more inclusive online events is especially important as multiple scientific organizations have announced that they will continue offering an online version of their event when in-person conferences can resume

Health services research in patients with breast cancer (CAMISS-prospective): study protocol for an observational prospective study

BACKGROUND: Though breast cancer remains a major health problem, there is a lack of information on health care provided to patients with this disease and associated costs. In addition, there is a need to update and validate risk stratification tools in Spain. Our purpose is to evaluate the health services provided for breast cancer in Spain, from screening and diagnosis to treatment and prognosis. METHODS: Prospective cohort study involving 13 hospitals in Spain with a follow-up period of up to 5 years after diagnostic biopsy. Eligibility criteria: Patients diagnosed with breast cancer between April 2013 and May 2015 that have consented to participate in the study. DATA COLLECTION: Data will be collected on the following: pre-intervention medical history, biological, clinical, and sociodemographic characteristics, mode of cancer detection, hospital admission, treatment, and outcomes up to 5 years after initial treatment. Questionnaires about quality of life (EuroQoL EQ-5D-5 L, the European Organization For Research And Treatment Of Cancer Core Quality Of Life Questionnaire EORTC QLQ-C30 join to the specific breast cancer module (QLQ-BR23), as well as Hospital Anxiety and Depression Scale were completed by the patients before the beginning of the initial treatment and at the end of follow-up period, 2 years later. The end-points of the study were changes in health-related quality of life, recurrence, complications and readmissions at 2 and 5 years after initial treatment. STATISTICAL ANALYSIS: Descriptive statistics will be calculated and multivariate models will be used where appropriate to adjust for potential confounders. In order to create and validate a prediction model, split validation and bootstrapping will be performed. Cost analysis will be carried out from the perspective of a national health system. DISCUSSION: The results of this coordinated project are expected to generate scientifically valid and clinically and socially important information to inform the decision-making of managers and the authorities responsible for ensuring equality in care processes as well in health outcomes. For clinicians, clinical prediction rules will be developed which are expected to serve as the basis for the development of software applications