Using Terrestrial Laser Scanning for the Recognition and Promotion of High-Alpine Geomorphosites

International audienceHigh-alpine geomorphosites are poorly understood and developed, mostly because of the heavy constraints of high mountain areas. Meanwhile, they are geoheritage areas that are often extremely vulnerable to global warming: glaciers and permafrost areas are currently affected by major changes due to increasing air temperature. To deal with the high spatial variability of landforms and processes, research on alpine geomorphosites often needs the use of advanced methods of high-resolution topography, among which terrestrial laser scanning plays an increasingly crucial role. Carried out on some tenth of high-elevation sites across the Alps since the beginning of the 2000s, this method is particularly interesting for the recognition and development of high-alpine geomorphosites. Indeed, it can be implemented for identifying and characterizing the geomorphic objects (survey, monitoring and mapping), helping planning and protection policies and serving geotouristic development (communication about the processes involved, basis for documents)

Stability monitoring of high Alpine infrastructure by terrestrial laser scanning

International audienceRock mass movements are dominant in the morphodynamics of high Alpine rock slopes and are at the origin of significant risks for people who attend these areas and for infrastructures that are built on (e.g. huts, cable cars). These risks are increasing because of permafrost degradation and glacier retreat as consequences of the global warming. These two factors may affect slope stability by changing mechanical properties of the interstitial ice and modifying the mechanical constraints in these rock slopes. The monitoring of rock slopes is thus an essential element for risk management. Our study focuses on two particularly active areas of the Western Alps: the lower Arête des Cosmiques (3613 m a.s.l., Mont Blanc massif, France) on which is located the very popular Refuge des Cosmiques, and the Col des Gentianes (2894 m a.s.l., Valais, Switzerland) where is located a cable car station. Discussed on the basis of geophysical and glaciological data, the evolutions monitored by terrestrial laser scanning probably result from the combination between permafrost activity/degradation and glacier shrinkage

Camera orientation, calibration and inverse perspective with uncertainties: A Bayesian method applied to area estimation from diverse photographs

This is the author accepted manuscript. The final version is available from the publisher via the DOI in this recordSome map data is copyrighted by OpenStreetMap contributors and available from https://www.openstreetmap.org.Large collections of images have become readily available through modern digital catalogs, from sources as diverse as historical photographs, aerial surveys, or user-contributed pictures. Exploiting the quantitative information present in such wide-ranging collections can greatly benefit studies that follow the evolution of landscape features over decades, such as measuring areas of glaciers to study their shrinking under climate change. However, many available images were taken with low-quality lenses and unknown camera parameters. Useful quantitative data may still be extracted, but it becomes important to both account for imperfect optics, and estimate the uncertainty of the derived quantities. In this paper, we present a method to address both these goals, and apply it to the estimation of the area of a landscape feature traced as a polygon on the image of interest. The technique is based on a Bayesian formulation of the camera calibration problem. First, the probability density function (PDF) of the unknown camera parameters is determined for the image, based on matches between 2D (image) and 3D (world) points together with any available prior information. In a second step, the posterior distribution of the feature area of interest is derived from the PDF of camera parameters. In this step, we also model systematic errors arising in the polygon tracing process, as well as uncertainties in the digital elevation model. The resulting area PDF therefore accounts for most sources of uncertainty. We present validation experiments, and show that the model produces accurate and consistent results. We also demonstrate that in some cases, accounting for optical lens distortions is crucial for accurate area determination with consumer-grade lenses. The technique can be applied to many other types of quantitative features to be extracted from photographs when careful error estimation is important.Agence Nationale de la Recherche (ANR

Using Terrestrial Laser Scanning for the Recognition and Promotion of High-Alpine Geomorphosites

High-alpine geomorphosites are poorly understood and developed, mostly because of the heavy constraints of high mountain areas. Meanwhile, they are geoheritage areas that are often extremely vulnerable to global warming: glaciers and permafrost areas are currently affected by major changes due to increasing air temperature. To deal with the high spatial variability of landforms and processes, research on alpine geomorphosites often needs the use of advanced methods of high-resolution topography, among which terrestrial laser scanning plays an increasingly crucial role. Carried out on some tenth of high-elevation sites across the Alps since the beginning of the 2000s, this method is particularly interesting for the recognition and development of high-alpine geomorphosites. Indeed, it can be implemented for identifying and characterizing the geomorphic objects (survey, monitoring and mapping), helping planning and protection policies and serving geotouristic development (communication about the processes involved, basis for documents)

Brief communication: 3-D reconstruction of a collapsed rock pillar from Web-retrieved images and terrestrial lidar data – the 2005 event of the west face of the Drus (Mont Blanc massif)

In June 2005, a series of major rockfall events completely wiped out the Bonatti Pillar located in the legendary Drus west face (Mont Blanc massif, France). Terrestrial lidar scans of the west face were acquired after this event, but no pre-event point cloud is available. Thus, in order to reconstruct the volume and the shape of the collapsed blocks, a 3-D model has been built using photogrammetry (structure-from-motion (SfM) algorithms) based on 30 pictures collected on the Web. All these pictures were taken between September 2003 and May 2005. We then reconstructed the shape and volume of the fallen compartment by comparing the SfM model with terrestrial lidar data acquired in October 2005 and November 2011. The volume is calculated to 292680m3 (±5.6%). This result is close to the value previously assessed by Ravanel and Deline (2008) for this same rock avalanche (265000±10000m3). The difference between these two estimations can be explained by the rounded shape of the volume determined by photogrammetry, which may lead to a volume overestimation. However it is not excluded that the volume calculated by Ravanel and Deline (2008) is slightly underestimated, the thickness of the blocks having been assessed manually from historical photographs

Recent evolution of an ice‐cored moraine at the Gentianes Pass, Valais Alps, Switzerland

International audienceLateral moraines located in permafrost environments often preserve large amounts of both glacier and periglacial ice. To understand how ice‐cored moraines located in high alpine environments evolve in a context of both glacier retreat and permafrost degradation, we performed 11 terrestrial laser‐scanning measurement campaigns between 2007 and 2014 on a highly anthropogenic overprinted moraine prone to instability. Resulting comparison of the subsequent 3D models allowed to qualitatively and quantitatively analyze the morphological evolution of the moraine. The comparisons indicate a very high geomorphic activity of the moraine including large areas affected by downslope movements of blocks and 10 landslides with a volume between 24 ± 1 and 1,138 ± 47 m3. Data also indicated a very strong ice melt with a loss of ice thickness locally reaching 17.7 m at the foot of the moraine. These results, compared with resistivity and thermal measurements of the ground, suggest the combined role of ice loss at the foot of the moraine and the permafrost activity/warming in triggering these processes

FYVE-Dependent Endosomal Targeting of an Arrestin-Related Protein in Amoeba

International audienceBACKGROUND: Visual and β-arrestins are scaffolding proteins involved in the regulation of receptor-dependent intracellular signaling and their trafficking. The arrestin superfamilly includes several arrestin domain-containing proteins and the structurally related protein Vps26. In Dictyostelium discoideum, the arrestin-domain containing proteins form a family of six members, namely AdcA to -F. In contrast to canonical arrestins, Dictyostelium Adc proteins show a more complex architecture, as they possess, in addition to the arrestin core, other domains, such as C2, FYVE, LIM, MIT and SAM, which potentially mediate selective interactions with either lipids or proteins. METHODOLOGY AND PRINCIPAL FINDINGS: A detailed analysis of AdcA has been performed. AdcA extends on both sides of the arrestin core, in particular by a FYVE domain which mediates selective interactions with PI(3)P, as disclosed by intrinsic fluorescence measurements and lipid overlay assays. Localization studies showed an enrichment of tagged- and endogenous AdcA on the rim of early macropinosomes and phagosomes. This vesicular distribution relies on a functional FYVE domain. Our data also show that the arrestin core binds the ADP-ribosylation factor ArfA, the unique amoebal Arf member, in its GDP-bound conformation. SIGNIFICANCE: This work describes one of the 6 arrestin domain-containing proteins of Dictyostelium, a novel and atypical member of the arrestin clan. It provides the basis for a better understanding of arrestin-related protein involvement in trafficking processes and for further studies on the expanding roles of arrestins in eukaryotes

Remobilization of leaf S compounds and senescence in response to restricted sulphate supply during the vegetative stage of oilseed rape are affected by mineral N availability

The impact of sulphur limitation on the remobilization of endogenous S compounds during the rosette stage of oilseed rape, and the interactions with N availability on these processes, were examined using a long-term 34SO42− labelling method combined with a study of leaf senescence progression (using SAG12/Cab as a molecular indicator) and gene expression of the transporters, BnSultr4;1 and BnSultr4;2, involved in vacuolar sulphate efflux. After 51 d on hydroponic culture at 0.3 mM 34SO42− (1 atom% excess), the labelling was stopped and plants were subject for 28 d to High S-High N (HS-HN, control), Low S-High N (LS-HN) or Low S-Low N (LS-LN) conditions. Compared with the control, LS-HN plants showed delayed leaf senescence and, whilst the shoot growth and the foliar soluble protein amounts were not affected, S, 34S, and SO42− amounts in the old leaves declined rapidly and were associated with the up-regulation of BnSultr4;1. In LS-LN plants, shoot growth was reduced, leaf senescence was accelerated, and the rapid S mobilization in old leaves was accompanied by decreased 34S and SO42−, higher protein mobilization, and up-regulation of BnSultr4;2, but without any change of expression of BnSultr4;1. The data suggest that to sustain the S demand for growth under S restriction (i) vacuolar SO42− is specifically remobilized in LS-HN conditions without any acceleration of leaf senescence, (ii) SO42− mobilization is related to an up-regulation of BnSultr4;1 and/or BnSultr4;2 expression, and (iii) the relationship between sulphate mobilization and up-regulation of expression of BnSultr4 genes is specifically dependent on the N availability

Ergothioneine Biosynthesis and Functionality in the Opportunistic Fungal Pathogen, Aspergillus fumigatus.

Ergothioneine (EGT; 2-mercaptohistidine trimethylbetaine) is a trimethylated and sulphurised histidine derivative which exhibits antioxidant properties. Here we report that deletion of Aspergillus fumigatus egtA (AFUA_2G15650), which encodes a trimodular enzyme, abrogated EGT biosynthesis in this opportunistic pathogen. EGT biosynthetic deficiency in A. fumigatus significantly reduced resistance to elevated H2O2 and menadione, respectively, impaired gliotoxin production and resulted in attenuated conidiation. Quantitative proteomic analysis revealed substantial proteomic remodelling in ΔegtA compared to wild-type under both basal and ROS conditions, whereby the abundance of 290 proteins was altered. Specifically, the reciprocal differential abundance of cystathionine γ-synthase and β-lyase, respectively, influenced cystathionine availability to effect EGT biosynthesis. A combined deficiency in EGT biosynthesis and the oxidative stress response regulator Yap1, which led to extreme oxidative stress susceptibility, decreased resistance to heavy metals and production of the extracellular siderophore triacetylfusarinine C and increased accumulation of the intracellular siderophore ferricrocin. EGT dissipated H2O2 in vitro, and elevated intracellular GSH levels accompanied abrogation of EGT biosynthesis. EGT deficiency only decreased resistance to high H2O2 levels which suggests functionality as an auxiliary antioxidant, required for growth at elevated oxidative stress conditions. Combined, these data reveal new interactions between cellular redox homeostasis, secondary metabolism and metal ion homeostasis

Metabolic Adaptation of Ralstonia solanacearum during Plant Infection: A Methionine Biosynthesis Case Study

MetE and MetH are two distinct enzymes that catalyze a similar biochemical reaction during the last step of methionine biosynthesis, MetH being a cobalamin-dependent enzyme whereas MetE activity is cobalamin-independent. In this work, we show that the last step of methionine synthesis in the plant pathogen Ralstonia solanacearum is under the transcriptional control of the master pathogenicity regulator HrpG. This control is exerted essentially on metE expression through the intermediate regulator MetR. Expression of metE is strongly and specifically induced in the presence of plant cells in a hrpG- and metR-dependent manner. metE and metR mutants are not auxotrophic for methionine and not affected for growth inside the plant but produce significantly reduced disease symptoms on tomato whereas disruption of metH has no impact on pathogenicity. The finding that the pathogen preferentially induces metE expression rather than metH in the presence of plant cells is indicative of a probable metabolic adaptation to physiological host conditions since this induction of metE occurs in an environment in which cobalamin, the required co-factor for MetH, is absent. It also shows that MetE and MetH are not functionally redundant and are deployed during specific stages of the bacteria lifecycle, the expression of metE and metH being controlled by multiple and distinct signals