Enhancing laboratory capacities in the Caribbean for better animal health regional surveillance

The Caribbean Animal Health Network (CaribVET) is a collaboration network of veterinary services, laboratories, research institutes and regional/international organizations in the Caribbean. Its goal is to improve animal and veterinary public health in the 32 Caribbean countries and territories. In the past, CaribVET had evaluated 34 CARICOM diagnostic laboratories and organized several workshops on IATA regulations and on diagnostic techniques. CaribVET coordinated simulation exercises on Avian Influenza samples' shipment and inter-laboratory assays on Classical Swine Fever diagnostic. The 'Laboratory Quality Assurance and Diagnosis Working Group ' (WG), created in 2011, gathers main actors involved in diagnostic and laboratory activities and meets every 3-4 months physically or virtually. The WG (1) regularly updates diagnostic capabilities and capacities in the region; (2) identifies training needs, promotes and strengthens links with reference labs; (3) provides guidance for the development of a regional network of laboratories while promoting the exchange of data, protocols, materials, and human resources; (4) promotes the implementation of quality assurance in veterinary diagnostic laboratories; and (5) supports the logistics of inter-laboratory assays. Recent achievements include (1) the development of an online database of laboratories in the Americas (CaribVET, CIRAD Guadeloupe, OIE collaboration); (2) signature of a letter of understanding between OIE and CaribVET to develop joint activities in accordance to both structures' recommendations; and (3) organization of a workshop on diagnostics of swine influenza and quality assurance in Guadeloupe within the FAO technical cooperation project on swine influenza surveillance. These coordinated activities reinforce diagnostic capacities and capabilities in the Caribbean which are essential for efficient surveillance of animal health. (Texte intégral

Is the distribution of Amblyomma variegatum influenced by interspecific competition with Amblyomma hebraeum? Preliminary study: distribution range in Mozambique

Widely spread in intertropical and subtropical areas of Africa, the southern distribution of Amblyomma variegatum in Mozambique seems to have its limit around the Save River, at the 22nd parallel South. This area also corresponds to the northern limit of another Amblyomma tick, A. hebraeum. Several authors use to explain the absence of A. variegatum more southwards by an interspecific competition with A. hebraeum. Whereas only A. variegatum is known to favor dermatophilosis, a skin disease that can cause important burden in ruminants, both species are good vectors of Ehrlichia ruminantium, the bacteria causing heartwater, a fatal disease of ruminants representing a major constraint for livestock development in Africa. However, their vector competence may be different and vary according to the strain of E. ruminantium. As a result, the bio-ecology and distribution of those vectors in the field may result in specific epidemiological patterns for the diseases they transmit and are important to understand to adopt proper sanitary control and prevention measures. Little information on current (last decade) Amblyomma distribution in Mozambique is available, and little is known on the factors influencing their distribution. More especially, the existence of a distribution overlap, as observed in Zimbabwe is not known in Mozambique. Indeed the extent of this overlap and the distribution of A. variegatum and A. hebraeum in this particular area represent useful information to assess potential interspecific competition. The objective of this study is to assess the current distribution of A. variegatum and A. hebraeum in this country and to better locate and characterize species limitation ranges around the Save River with a view to further identify the factors influencing their distribution. First, an exhaustive literature review was made to collect all published and unpublished data relating to the distribution of A. variegatum and A. hebraeum in Africa. A field entomological survey was then conducted in Mozambique between February and April 2012 to identify ticks collected on cattle, in the Inhambane, Manica and Sofala provinces, nearby the Save River. Sampling sites included dip-tanks and corridors, used for acaricides treatment by neighboring farmers, as well as farms which were selected by the local Veterinary Service. In each study site, about 60 animals were carefully examined for ticks presence and abundance, mostly in corridors and on 10 laid-down animals most heavily infested by ticks. Ticks, males and females, were collected to confirm species identification. Data on domestic ruminant population and movements as well as breeding practices including tick control were also collected through interviews with farmers and local and central veterinary services. The epidemiological unit is the sampling site. A site was considered positive for a given tick species if at least one animal was infested by at least one tick of that species, otherwise, it was considered negative. To better assess species presence in their limit of distribution, an attempt was made to interpret presence and absence data to distinguish well-established or occasionally present population and likely not detected or absent populations. This was done considering sampling method, tick abundance, study period against phenology, as well as other information: last acaricide treatment, animal introduction, etc. Data were entered in Access database and displayed on a Geographic Information System, ArcMap9.3 A map of the current A. variegatum and A. hebraeum distribution was produced and included 103 positive sites out of 113 sampled between 2000 and 2012. The field survey conducted in 30 study sites evidenced a distribution overlap of 30km (western side) to 100km (eastern side) width, along a slanting line northwest-southeast accross the Save River, where both species were found in three sites and coinfestation of few animals was observed. The current distribution o

Transmission measurement at 10.6 microns of Te2As3Se5 rib-waveguides on As2S3 substrate

The feasibility of chalcogenide rib waveguides working at lambda = 10.6 microns has been demonstrated. The waveguides comprised a several microns thick Te2As3Se5 film deposited by thermal evaporation on a polished As2S3 glass substrate and further etched by physical etching in Ar or CF4/O2 atmosphere. Output images at 10.6 microns and some propagation losses roughly estimated at 10dB/cm proved that the obtained structures behaved as channel waveguides with a good lateral confinement of the light. The work opens the doors to the realisation of components able to work in the mid and thermal infrared up to 20 microns and even more.Comment: The following article appeared in Vigreux-Bercovici et al., Appl. Phys. Lett. 90, 011110 (2007) and may be found at http://link.aip.org/link/?apl/90/01111

VLBA images of the precessing jet of LSI+61303

Context: In 2004, changes in the radio morphology of the Be/X-ray binary system LSI+61303 suggested that it is a precessing microquasar. In 2006, a set of VLBA observations performed throughout the entire orbit of the system were not used to study its precession because the changes in radio morphology could tentatively be explained by the alternative pulsar model. However, a recent radio spectral index data analysis has confirmed the predictions of the two-peak microquasar model, which therefore does apply in LSI+61303. Aims: We revisit the set of VLBA observations performed throughout the orbit to determine the precession period and improve our understanding of the physical mechanism behind the precession. Methods: By reanalyzing the VLBA data set, we improve the dynamic range of images by a factor of four, using self-calibration. Different fitting techniques are used and compared to determine the peak positions in phase-referenced maps. Results: The improved dynamic range shows that in addition to the images with a one-sided structure, there are several images with a double-sided structure. The astrometry indicates that the peak in consecutive images for the whole set of observations describes a well-defined ellipse, 6-7 times larger than the orbit, with a period of about 28 d. Conclusions: A double-sided structure is not expected to be formed from the expanding shocked wind predicted in the pulsar scenario. In contrast, a precessing microquasar model can explain the double- and one-sided structures in terms of variable Doppler boosting. The ellipse defined by the astrometry could be the cross-section of the precession cone, at the distance of the 8.4 GHz-core of the steady jet, and 28d the precession period.Comment: 7 pages, 5 figures, Accepted for publication in Astronomy and Astrophysics, added references for sect.

Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections

Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the development of the infection, but the mechanisms involved remain unclear. Many bacterial pathogens target the follicle-associated epithelium, which overlies Peyer's patches (PPs), cross the intestinal barrier through M cells and are captured by mucosal macrophages. Here, translocation across M cells, as well as survival and proliferation of EHEC strains within THP-1 macrophages were investigated using EHEC O157:H7 reference strains, isogenic mutants, and 15 EHEC strains isolated from HC/HUS patients. We showed for the first time that E. coli O157:H7 strains are able to interact in vivo with murine PPs, to translocate ex vivo through murine ileal mucosa with PPs and across an in vitro human M cell model. EHEC strains are also able to survive and to produce Stx in macrophages, which induce cell apoptosis and Stx release. In conclusion, our results suggest that the uptake of EHEC by M cells and underlying macrophages in the PP may be a critical step in Stx translocation and release in vivo. A new model for EHEC infection in humans is proposed that could help in a fuller understanding of EHEC-associated diseases

Technology challenges for space interferometry: the option of mid-infrared integrated optics

Nulling interferometry is a technique providing high angular resolution which is the core of the space missions Darwin and the Terrestrail Planet Finder. The first objective is to reach a deep degree of starlight cancelation in the range 6 -- 20 microns, in order to observe and to characterize the signal from an Earth-like planet. Among the numerous technological challenges involved in these missions, the question of the beam combination and wavefront filtering has an important place. A single-mode integrated optics (IO) beam combiner could support both the functions of filtering and the interferometric combination, simplifying the instrumental design. Such a perspective has been explored in this work within the project Integrated Optics for Darwin (IODA), which aims at developing a first IO combiner in the mid-infrared. The solutions reviewed here to manufacture the combiner are based on infrared dielectric materials on one side, and on metallic conductive waveguides on the other side. With this work, additional inputs are offered to pursue the investigation on mid-infrared photonics devices.Comment: Accepted in Adv. in Space Researc

High catalytic efficiency of palladium nanoparticles immobilized in a polymer membrane containing poly(ionic liquid) in Suzuki–Miyaura cross-coupling reaction

The elaboration of a polymeric catalytic membrane containing palladium nanoparticles is presented. The membrane was prepared using a photo-grafting process with imidazolium-based ionic liquid monomers as modifying agent and microPES® as support membrane. Ionic liquid serves as a stabilizer and immobilizer for the catalytic species, i.e. palladium nanoparticles. The Suzuki–Miyaura cross-coupling reaction was carried out on the catalytic membrane in flow-through configuration. Complete conversion was achieved in 10 s through one single filtration, without formation of byproducts. The apparent reaction rate constant was three orders of magnitude greater than in a batch reactor. No catalyst leaching was detected. This membrane offers the possibility of continuous production with no need for a separation step of the catalyst from the reaction medium

VLBI study of maser kinematics in high-mass SFRs. I. G16.59-0.05

The present paper focuses on the high-mass star-forming region G16.59-0.05. Methods: Using the VLBA and the EVN arrays, we conducted phase-referenced observations of the three most powerful maser species in G16.59-0.05: H2O at 22.2 GHz (4 epochs), CH3OH at 6.7 GHz (3 epochs), and OH at 1.665 GHz (1 epoch). In addition, we performed high-resolution (> 0".1), high-sensitivity (< 0.1 mJy) VLA observations of the radio continuum emission from the star-forming region at 1.3 and 3.6 cm. Results: This is the first work to report accurate measurements of the "relative" proper motions of the 6.7 GHz CH3OH masers. The different spatial and 3-D velocity distribution clearly indicate that the 22 GHz water and 6.7 GHz methanol masers are tracing different kinematic environments. The bipolar distribution of 6.7 GHz maser l.o.s. velocities and the regular pattern of observed proper motions suggest that these masers are tracing rotation around a central mass of about 35 solar masses. The flattened spatial distribution of the 6.7 GHz masers, oriented NW-SE, suggests that they can originate in a disk/toroid rotating around the massive YSO which drives the 12CO(2-1) outflow, oriented NE-SW, observed on arcsec scale. The extended, radio continuum source observed close to the 6.7 GHz masers could be excited by a wide-angle wind emitted from the YSO associated with the methanol masers, and such a wind is proven to be sufficiently energetic to drive the NE-SW 12CO(2-1) outflow. The H2O masers distribute across a region offset about 0".5 to the NW of the CH3OH masers, in the same area where emission of high-density molecular tracers, typical of HMCs, was detected. We postulate that a distinct YSO, possibly in an earlier evolutionary phase than that exciting the methanol masers, is responsible for the excitation of the water masers and the HMC molecular lines. (Abridged)Comment: 20 pages, 8 figures, 3 tables, accepted by Astronomy and Astrophysic

Parapatric distribution and sexual competition between two tick species, [i]Amblyomma variegatum[/i] and [i]A. hebraeum[/i] (Acari, Ixodidae), in Mozambique

[b]Background[/b]: [i]Amblyomma variegatum[/i] and [i]A. hebraeum[/i] are two ticks of veterinary and human health importance in south-east Africa. In Zimbabwe they occupy parapatric (marginally overlapping and juxtaposed) distributions. Understanding the mechanisms behind this parapatry is essential for predicting the spatio-temporal dynamics of Amblyomma spp. and the impacts of associated diseases. It has been hypothesized that exclusive competition between these species results from competition at the levels of male signal reception (attraction-aggregation-attachment pheromones) or sexual competition for mates. This hypothesis predicts that the parapatry described in Zimbabwe could also be present in other countries in the region. [br/][b]Methods[/b]: To explore this competitive exclusion hypothesis we conducted field surveys at the two species' range limits in Mozambique to identify areas of sympatry (overlapping areas) and to study potential interactions (communicative and reproductive interference effects) in those areas. At sympatric sites, hetero-specific mating pairs were collected and inter-specific attractiveness/repellent effects acting at long and short distances were assessed by analyzing species co-occurrences on co-infested herds and co-infested hosts.[br/] [b]Results[/b]: Co-occurrences of both species at sampling sites were infrequent and localized in areas where both tick and host densities were low. At sympatric sites, high percentages of individuals of both species shared attachment sites on hosts and inter-specific mating rates were high. Although cross-mating rates were not significantly different for[i] A. variegatum[/i] and [i]A. hebraeum[/i] females, attraction towards hetero-specific males was greater for [i]A. hebraeum[/i] females than for A. variegatum females and we observed small asymmetrical repellent effects between males at attachment sites.[br/][b]Conclusions[/b]: Our observations suggest near-symmetrical reproductive interference between [i]A. variegatum[/i] and [i]A. hebraeum[/i], despite between-species differences in the strength of reproductive isolation barriers acting at the aggregation, fixation and partner contact levels. Theoretical models predict that sexual competition coupled with hybrid inviability, greatly reduces the probability of one species becoming established in an otherwise suitable location when the other species is already established. This mechanism can explain why the parapatric boundary in Mozambique has formed within an area of low tick densities and relatively infrequent host-mediated dispersal events

Anti-plasmodial polyvalent interactions in Artemisia annua L. aqueous extract – possible synergistic and resistance mechanisms

Artemisia annua hot water infusion (tea) has been used in in vitro experiments against P. falciparum malaria parasites to test potency relative to equivalent pure artemisinin. High performance liquid chromatography (HPLC) and mass spectrometric analyses were employed to determine the metabolite profile of tea including the concentrations of artemisinin (47.5±0.8 mg L-1), dihydroartemisinic acid (70.0±0.3 mg L-1), arteannuin B (1.3±0.0 mg L-1), isovitexin (105.0±7.2 mg L-1) and a range of polyphenolic acids. The tea extract, purified compounds from the extract, and the combination of artemisinin with the purified compounds were tested against chloroquine sensitive and chloroquine resistant strains of P. falciparum using the DNA-intercalative SYBR Green I assay. The results of these in vitro tests and of isobologram analyses of combination effects showed mild to strong antagonistic interactions between artemisinin and the compounds (9-epi-artemisinin and artemisitene) extracted from A. annua with significant (IC50 <1 μM) anti-plasmodial activities for the combination range evaluated. Mono-caffeoylquinic acids, tri-caffeoylquinic acid, artemisinic acid and arteannuin B showed additive interaction while rosmarinic acid showed synergistic interaction with artemisinin in the chloroquine sensitive strain at a combination ratio of 1:3 (artemisinin to purified compound). In the chloroquine resistant parasite, using the same ratio, these compounds strongly antagonised artemisinin anti-plasmodial activity with the exception of arteannuin B, which was synergistic. This result would suggest a mechanism targeting parasite resistance defenses for arteannuin B’s potentiation of artemisinin