Comparison of Antibodies to Detect Uroplakin in Urothelial Carcinomas

Immunohistochemistry for Uroplakin (UP) II and III is used to determine urothelial origin of carcinomas of unknown primary site and are especially valuable to differentiate urothelial carcinomas (UCs) from lung squamous cell carcinomas and prostate carcinomas. In the Nordic immunohistochemical Quality Control assessment scheme, only 45% of the participants obtained a sufficient staining result for UP. Primary antibodies (Abs) against UPII were most successful with a pass rate of 86%. No Abs against UPIII provided sufficient staining results. A comparative study was carried out on a larger cohort of tissue samples with optimized methods for the UPII mouse monoclonal antibody (mmAb) clone BC21, UPIII mmAb clone AU-1, and rabbit monoclonal antibody (rmAb) clone SP73 to evaluate the performance in a standardized way. Tissue microarrays containing 58 UCs, 111 non-UCs, and 20 normal tissues were included. The UP stains were evaluated by using H-score. Based on H-scores, samples were categorized as high-expressor (150 to 300), moderate-expressor (10 to 149), low-expressor (1 to 9), and negative (150 for the UPII Ab. The 2 UPIII Abs gave an analytical specificity of 100% compared with 97% for the UPII Ab being positive in 2 ovarian carcinomas and 1 cervical squamous cell carcinoma

Tilberedning og Afsætning af saltet Flæsk.

Tilberedning og Afsætning af saltet Flæsk

Implementing a care pathway for elderly patients, a comparative qualitative process evaluation in primary care

Background: In Central Norway a generic care pathway was developed in collaboration between general hospitals and primary care with the intention of implementing it into everyday practice. The care pathway targeted elderly patients who were in need of home care services after discharge from hospital. The aim of the present study was to investigate the implementation process of the care pathway by comparing the experiences of health care professionals and managers in home care services between the participating municipalities. Methods: This was a qualitative comparative process evaluation using data from individual and focus group interviews. The Normalization Process Theory, which provides a framework for understanding how a new intervention becomes part of normal practice, was applied in our analysis. Results: In all of the municipalities there were expectations that the generic care pathway would improve care coordination and quality of follow-up, but a substantial amount of work was needed to make the regular home care staff understand how to use the care pathway. Other factors of importance for successful implementation were involvement of the executive municipal management, strong managerial focus on creating engagement and commitment among all professional groups, practical facilitation of work processes, and a stable organisation without major competing priorities. At the end of the project period, the pathway was integrated in daily practice in two of the six municipalities. In these municipalities the care pathway was found to have the potential of structuring the provision of home care services and collaboration with the GPs, and serving as a management tool to effect change and improve knowledge and skills. Conclusion: The generic care pathway for elderly patients has a potential of improving follow-up in primary care by meeting professional and managerial needs for improved quality of care, as well as more efficient organisation of home care services. However, implementation of this complex intervention in full-time running organisations was demanding and required comprehensive and prolonged efforts in all levels of the organisation. Studies on implementation of such complex interventions should therefore have a long follow-up time to identify whether the intervention becomes integrated into everyday practice

Санитарно-эпидемиологическая экспертиза импортной пищевой продукции и продовольственного сырья как составляющая профилактического направления транспортной медицины

Санітарно епідеміологічна експертиза імпортної харчової продукції й продовольчої сировини (далі експертиза) є одним з пріоритетних напрямів діяльності транспортних підрозділів санепідслужби. Здійснюючи функцію по контролю, виявленню та попередженню впливу небезпечних факторів, пов’язаних з перевезенням вантажів, вона є однією зі складових частин профілактичного напряму транспортної медицини. Експертиза імпортованої продукції харчового призначення нерозривно пов’язана та базується на загальних принципах всієї державної політики у сфері безпеки харчової продукції та продовольчої сировини. Основою для проведення усього комплексу робіт санітарно епідеміологічного направлення є нормативна база, яка була сформована в нашій країні кілька десятиліть назад. Механізм визначення безпеки продукції та ті критерії оцінки, які були закладені в її основі, вимагають перегляду у відповідності з вимогами сьогодення та враховуючи розвиток міжнародних відносин. Зроблені деякі кроки у цьому напрямку. Наприклад, визначення експертизи у законі «Про внесення змін до закону України «Про якість та безпеку харчових продуктів та продовольчої сировини» поряд зі встановленням відповідності продукції нормативним вимогам передбачає оцінку ризику дії шкідливих факторів у процесі обігу харчових продуктів, що відповідає сучас ним вимогам до вирішення задач профілактичного напрямку, запобігання шкідливого впливу факторів, керування санітарно-епідеміологічною ситуацією взагалі та на етапі транспортування харчових грузів зокрема. Такий підхід потрібно враховувати при подальшому необхідному перегляді та формуванні нової нормативної бази.Sanіtarу epidemiological examination of imported foodstuffs and edible raw materials (then «examination») is one of the priority directions of transport sanіtarу epidemiological servise. Examination controls, discovers and prevents an influence of dangerous factors while in transportation of loads. So it’s one of the component of the transport medicine preventive activity. Examination of import foodstuffs and edible raw materials inseparably linked with and based on general principles of the food safety state policy. The foundation of the sanitary service work is the normative base, that was formed in our country about twenty years ago. It is necessary to review the mechanism of the food safety determination and its criteria in accordance with requirements of present day time and with account of the development of the international relations. One of taken steps in this direction is a characteristic of examination in law «About contributing the modification to law of the Ukraine «About quality and safety of foodstuffs and foodraw materials» where along with determination of the products correspondence to the normative requirements is provided forrisk assessment of the harmful factors in process of the turn of the food stuffs. Such approach corresponds with modern requests to decision of the prophylactic problems, prevention bad influence dangerous factors, management sanіtarу epidemiological situation in general and in step of transportation food cargo in particular. And it should be taken into account during the further necessary process of revision and forming the new normative base

The Effect of How Outcomes Are Framed on Decisions about Whether to Take Antihypertensive Medication: A Randomized Trial

BACKGROUND: We conducted an Internet-based randomized trial comparing three valence framing presentations of the benefits of antihypertensive medication in preventing cardiovascular disease (CVD) for people with newly diagnosed hypertension to determine which framing presentation resulted in choices most consistent with participants' values. METHODS AND FINDINGS: In this second in a series of televised trials in cooperation with the Norwegian Broadcasting Company, adult volunteers rated the relative importance of the consequences of taking antihypertensive medication using visual analogue scales (VAS). Participants viewed information (or no information) to which they were randomized and decided whether or not to take medication. We compared positive framing over 10 years (the number escaping CVD per 1000); negative framing over 10 years (the number that will have CVD) and negative framing per year over 10 years of the effects of antihypertensive medication on the 10-year risk for CVD for a 40 year-old man with newly diagnosed hypertension without other risk factors. Finally, all participants were shown all presentations and detailed patient information about hypertension and were asked to decide again. We calculated a relative importance score (RIS) by subtracting the VAS-scores for the undesirable consequences of antihypertensive medication from the VAS-score for the benefit of CVD risk reduction. We used logistic regression to determine the association between participants' RIS and their choice. 1,528 participants completed the study. The statistically significant differences between the groups in the likelihood of choosing to take antihypertensive medication in relation to different values (RIS) increased as the RIS increased. Positively framed information lead to decisions most consistent with those made by everyone for the second, more fully informed decision. There was a statistically significant decrease in deciding to take antihypertensives on the second decision, both within groups and overall. CONCLUSIONS: For decisions about taking antihypertensive medication for people with a relatively low baseline risk of CVD (70 per 1000 over 10 years), both positive and negative framing resulted in significantly more people deciding to take medication compared to what participants decided after being shown all three of the presentations. TRIAL REGISTRATION: International Standard Randomised Controlled Trial Number Register ISRCTN 33771631

Wandering behaviour prevents inter and intra oceanic speciation in a coastal pelagic fish

Small pelagic fishes have the ability to disperse over long distances and may present complex evolutionary histories. Here, Old World Anchovies (OWA) were used as a model system to understand genetic patterns and connectivity of fish between the Atlantic and Pacific basins. We surveyed 16 locations worldwide using mtDNA and 8 microsatellite loci for genetic parameters, and mtDNA (cyt b; 16S) and nuclear (RAG1; RAG2) regions for dating major lineage-splitting events within Engraulidae family. The OWA genetic divergences (0-0.4%) are compatible with intra-specific divergence, showing evidence of both ancient and contemporary admixture between the Pacific and Atlantic populations, enhanced by high asymmetrical migration from the Pacific to the Atlantic. The estimated divergence between Atlantic and Pacific anchovies (0.67 [0.53-0.80] Ma) matches a severe drop of sea temperature during the Gunz glacial stage of the Pleistocene. Our results support an alternative evolutionary scenario for the OWA, suggesting a coastal migration along south Asia, Middle East and eastern Africa continental platforms, followed by the colonization of the Atlantic via the Cape of the Good Hope.Portuguese Foundation for Science & Technology (FCT) [SFRH/BD/36600/2007]; FCT [UID/MAR/04292/2013, SFRH/BPD/65830/2009]; FCT strategic plan [UID/Multi/04326/2013]info:eu-repo/semantics/publishedVersio