176 research outputs found
Micromechanical modeling of the elastic behavior of unidirectional CVI SiC/SiC composites
International audienceThe elastic behavior of SiC/SiC composite is investigated at the scale of the tow through a micromechanical modeling taking into account the heterogeneous nature of the microstructure. The paper focuses on the sensitivity of transverse properties to the residual porosity resulting from the matrix infiltration process. The full analysis is presented stepwise, starting from the microstructural characterization to the study of the impact of pore shape and volume fraction. Various Volume Elements (VEs) of a virtual microstructure are randomly generated. Their microstructural properties are validated with respect to an experimental characterization based on high definition SEM observations of real materials, using various statistical descriptors. The linear elastic homogenization is performed using finite elements calculations for several VE sizes and boundary conditions. Important fluctuations of the apparent behavior, even for large VEs, reveal that scales are not separated. Nevertheless, a homogeneous equivalent behavior is estimated by averaging apparent behaviors of several VEs smaller than the Representative Volume Element (RVE). Therefore, the impact of the irregular shape of the pores on the overall properties is highlighted by comparison to a simpler cylindrical porous microstructure. Finally, different matrix infiltration qualities are simulated by several matrix thicknesses. A small increase in porosity volume fraction is shown to potentially lead to an important fall of transverse elastic moduli together with high stress concentrations
Identification of crystalline behavior on macroscopic response and local strain field analysis: application to alpha zirconium alloys
The purpose of this paper is to present an identification method of the crystalline behavior of a material from a mechanical test performed on a polycrystalline sample. Because of the lack of knowledge about its crystalline behavior, this method is applied to a Zirconium alloy. This identification is based on a finite element modeling of the microstructure, and the results are compared to both the macroscopic and the microscopic experimental results. On the microscopic scale, the plastic strains are obtained using a micro-extensometry technique and the crystalline orientation using an EBSD technique. In order to validate the method, an identification is performed with only two free parameters: the evolutions of the macroscopic and microscopic errors appear to be regular and exhibit a well-defined minimum so that the parameters can be clearly identified
Multiscale approach of mechanical behaviour of SiC/SiC composites: Elastic behaviour at the scale of the tow
SiC/SiC composites are candidates for structural applications at elevated temperatures in the context of the development of the 4th generation of nuclear reactors. A multiscale approach is under development to construct a predictive modelling of their complex mechanical behaviour due to their heterogeneous microstructure. This approach is based on two scale transitions: from the ïŹbres/matrix microstructure to the tow and from the tow to the woven composite, each scale presenting a signiïŹcant residual porosity. This paper focuses on the ïŹrst scale transition and on the modelling of the elastic behaviour of the tow at room temperature. A microstructural investigation of several tows in a 2D SiC/SiC specimen has been conducted using scanning electron microscopy to get statistical data on microstructural characteristics by image analysis in order to generate a virtual microstructure. The elastic problem of homogenisation is numerically solved by means of ïŹnite element techniques. The simulations performed on various volumes show noticeable ïŹuctuations of the apparent behaviour: so separation of length scales is not satisïŹed in this material. Nevertheless, this problem is neglected in a ïŹrst approximation and the homogeneous equivalent behaviour is evaluated by averaging the apparent behaviours of several volume elements â smaller than the Representative Volume Element (RVE) â called Statistical Volume Elements (SVEs). Finally, inïŹuence of porosity and poresâ morphology is quantiïŹed
The lytic transglycosylase MltB connects membrane homeostasis and in vivo fitness of Acinetobacter baumannii
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146339/1/mmi14000-sup-0001-Supinfo.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146339/2/mmi14000.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146339/3/mmi14000_am.pd
Integrating the Water Planetary Boundary With Water Management From Local to Global Scales
The planetary boundaries framework defines the "safe operating space for humanity" represented by nine global processes that can destabilize the Earth System if perturbed. The water planetary boundary attempts to provide a global limit to anthropogenic water cycle modifications, but it has been challenging to translate and apply it to the regional and local scales at which water problems and management typically occur. We develop a cross-scale approach by which the water planetary boundary could guide sustainable water management and governance at subglobal contexts defined by physical features (e.g., watershed or aquifer), political borders (e.g., city, nation, or group of nations), or commercial entities (e.g., corporation, trade group, or financial institution). The application of the water planetary boundary at these subglobal contexts occurs via two approaches: (i) calculating fair shares, in which local water cycle modifications are compared to that context's allocation of the global safe operating space, taking into account biophysical, socioeconomic, and ethical considerations; and (ii) defining a local safe operating space, in which interactions between water stores and Earth System components are used to define local boundaries required for sustaining the local water system in stable conditions, which we demonstrate with a case study of the Cienaga Grande de Santa Marta wetlands in Colombia. By harmonizing these two approaches, the water planetary boundary can ensure that water cycle modifications remain within both local and global boundaries and complement existing water management and governance approaches
Microwave studies of the fractional Josephson effect in HgTe-based Josephson junctions
The rise of topological phases of matter is strongly connected to their
potential to host Majorana bound states, a powerful ingredient in the search
for a robust, topologically protected, quantum information processing. In order
to produce such states, a method of choice is to induce superconductivity in
topological insulators. The engineering of the interplay between
superconductivity and the electronic properties of a topological insulator is a
challenging task and it is consequently very important to understand the
physics of simple superconducting devices such as Josephson junctions, in which
new topological properties are expected to emerge. In this article, we review
recent experiments investigating topological superconductivity in topological
insulators, using microwave excitation and detection techniques. More
precisely, we have fabricated and studied topological Josephson junctions made
of HgTe weak links in contact with two Al or Nb contacts. In such devices, we
have observed two signatures of the fractional Josephson effect, which is
expected to emerge from topologically-protected gapless Andreev bound states.
We first recall the theoretical background on topological Josephson junctions,
then move to the experimental observations. Then, we assess the topological
origin of the observed features and conclude with an outlook towards more
advanced microwave spectroscopy experiments, currently under development.Comment: Lectures given at the San Sebastian Topological Matter School 2017,
published in "Topological Matter. Springer Series in Solid-State Sciences,
vol 190. Springer
Inhibition of epidermoid carcinoma A431 cell growth and angiogenesis in nude mice by early and late treatment with a novel dextran derivative
Quorum Sensing Signaling Molecules Produced by Reference and Emerging Soft-Rot Bacteria (Dickeya and Pectobacterium spp.)
International audienceBACKGROUND: Several small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and Îł-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pectobacterium species including recent or emerging potato isolates. METHODOLOGY/PRINCIPAL FINDINGS: Using bacterial biosensors, immunoassay, and chromatographic analysis, we showed that soft-rot bacteria have the common ability to produce transiently during their exponential phase of growth the N-3-oxo-hexanoyl- or the N-3-oxo-octanoyl-l-homoserine lactones and a molecule of the autoinducer-2 family. Dickeya spp. produced in addition the indole-3-acetic acid in tryptophan-rich conditions. All these signaling molecules have been identified for the first time in the novel Dickeya solani species. In contrast, quinolone and Îł-amino butyrate signals were not identified and the corresponding synthases are not present in the available genomes of soft-rot bacteria. To determine if the variations of signal production according to growth phase could result from expression modifications of the corresponding synthase gene, the respective mRNA levels were estimated by reverse transcriptase-PCR. While the N-acyl-homoserine lactone production is systematically correlated to the synthase expression, that of the autoinducer-2 follows the expression of an enzyme upstream in the activated methyl cycle and providing its precursor, rather than the expression of its own synthase. CONCLUSIONS/SIGNIFICANCE: Despite sharing the S-adenosylmethionine precursor, no strong link was detected between the production kinetics or metabolic pathways of autoinducers-1 and -2. In contrast, the signaling pathway of autoinducer-2 seems to be switched off by the indole-3-acetic acid pathway under tryptophan control. It therefore appears that the two genera of soft-rot bacteria have similarities but also differences in the mechanisms of communication via the diffusible molecules. Our results designate autoinducer-1 lactones as the main targets for a global biocontrol of soft-rot bacteria communications, including those of emerging isolates
Comparative transcriptomic analysis reveals similarities and dissimilarities in saccharomyces cerevisiae wine strains response to nitrogen availability
Nitrogen levels in grape-juices are of major importance in winemaking ensuring adequate yeast growth and fermentation performance. Here we used a comparative transcriptome analysis to uncover wine yeasts responses to nitrogen availability during fermentation. Gene expression was assessed in three genetically and phenotypically divergent commercial wine strains (CEG, VL1 and QA23), under low (67 mg/L) and high nitrogen (670 mg/L) regimes, at three time points during fermentation (12h, 24h and 96h). Two-way ANOVA analysis of each fermentation condition led to the identification of genes whose expression was dependent on strain, fermentation stage and on the interaction of both factors. The high fermenter yeast strain QA23 was more clearly distinct from the other two strains, by differential expression of genes involved in flocculation, mitochondrial functions, energy generation and protein folding and stabilization. For all strains, higher transcriptional variability due to fermentation stage was seen in the high nitrogen fermentations. A positive correlation between maximum fermentation rate and the expression of genes involved in stress response was observed. The finding of common genes correlated with both fermentation activity and nitrogen up-take underlies the role of nitrogen on yeast fermentative fitness. The comparative analysis of genes differentially expressed between both fermentation conditions at 12h, where the main difference was the level of nitrogen available, showed the highest variability amongst strains revealing strain-specific responses. Nevertheless, we were able to identify a small set of genes whose expression profiles can quantitatively assess the common response of the yeast strains to varying nitrogen conditions. The use of three contrasting yeast strains in gene expression analysis prompts the identification of more reliable, accurate and reproducible biomarkers that will facilitate the diagnosis of deficiency of this nutrient in the grape-musts and the development of strategies to optimize yeast performance in industrial fermentations
Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers
Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants
- âŠ