Effet de différents systèmes de culture à couverture végétale sur le stockage du carbone dans un sol argileux des Hautes Terres de Madagascar

Stocker du carbone dans le sol permet d'améliorer ses propriétés physico-chimiques et de réduire les émissions de dioxyde de carbone vers l'atmosphère. L'effet des systèmes en semis direct avec couverture végétale (SCV) sur le stockage de C dans le sol est étudié sur un dispositif agronomique de longue durée (11 ans) à Antsirabe, Madagascar (16°C, 1 300 mm). Quatre systèmes sont étudiés: un système en labour conventionnel avec exportation des résidus de récolte [CT m/s, rotation maïs (Zea mays L.)-soja (Glycine max. L.)], et trois systèmes en SCV sans travail du sol, et avec restitution des résidus de récolte [NT m/s, rotation maïs-soja; NT m/m-d, rotation maïs-maïs avec une couverture végétale de Desmodium uncinatum; et NT h/s-k, rotation haricot (Phaseolus vulgaris)-soja avec une couverture végétale de Pennissetum clandestinum]. Le sol est très argileux, à faible capacité d'échange cationique mais possédant des propriétés andiques pouvant influencer les potentialités de stockage du C du sol. A 0-5 cm, les teneurs en C sont plus élevées sous SCV (NT m/s, NT m/m-d et NT his) que sous labour (CT m/s), et à 5-10 cm, elles sont plus élevées sous NT m/m-d et NT m/s que sous NT h/s-k et CT m/s. Le stockage annuel de C, à masse de sol équivalente, est de 0,69 et 1,01 mg C.ha-1.an-1, sous NT m/s et NT m/m-d pour l'horizon équivalent à 0-20 cm, alors qu'il n'y a pas d'effet SCV observé pour l'horizon équivalent à 0-40 cm. Ceci peut être dû à la fois à l'absence réelle de stockage comme à une variabilité initiale des teneurs en C dans les horizons de profondeurs, car le labour n'est effectué que jusqu'à 20 cm de profondeur. Les différences de stockage de C entre NT et CT dans la couche 0-20 cm sont essentiellement attribuées aux quantités beaucoup plus importantes de résidus organiques restituées par les systèmes NT par rapport au système labouré CT, mais on peut aussi envisager qu'une partie de cette différence soit le fait d'une perte de C par érosion sous labour. Les teneurs en macroagrégats stables (MA, 200-2 000 [mu]m) sont plus élevées sous NT m/s, NT h/s-k et NT m/m-d que sous CT m/s à 0-5 cm et à 5-10 cm. Cette teneur en MA est corrélée positivement (R = 0,408, p < 0,05, n = 24) avec la teneur en C du sol, ce qui pourrait induire (i) une amélioration de l'agrégation en fonction de l'augmentation de la teneur en C du sol et (ii) une protection du C se trouvant à l'intérieur de ces agrégats contre la minéralisation microbienne. Toutefois, la respirométrie ne montre pas une protection physique de C dans les sites de protection supérieurs à 200 [mu]m pour NT m/s et CT m/s. Dans cette étude, le C stocké dans le sol pourrait alors être protégé contre la minéralisation par d'autres processus comme l'adsorption sur les colloïdes du sol ou la recalcitrance biochimique de la matière organique du sol. (Résumé d'auteur

No selection on immunological markers in response to a highly virulent pathogen in an Arctic breeding bird

In natural populations, epidemics provide opportunities to look for intense natural selection on genes coding for life history and immune or other physiological traits. If the populations being considered are of management or conservation concern, then identifying the traits under selection (or ‘markers’) might provide insights into possible intervention strategies during epidemics. We assessed potential for selection on multiple immune and life history traits of Arctic breeding common eiders (Somateria mollissima) during annual avian cholera outbreaks (summers of 2006, 2007 & 2008). We measured prelaying body condition, immune traits, and subsequent reproductive investment (i.e., clutch size) and survival of female common eiders and whether they were infected with Pasteurella multocida, the causative agent of avian cholera. We found no clear and consistent evidence of directional selection on immune traits; however, infected birds had higher levels of haptoglobin than uninfected birds. Also, females that laid larger clutches had slightly lower immune responses during the prelaying period reflecting possible downregulation of the immune system to support higher costs of reproduction. This supports a recent study indicating that birds investing in larger clutches were more likely to die from avian cholera and points to a possible management option to maximize female survival during outbreaks

Effect on comfort of administering bubble-humidified or dry oxygen: the Oxyrea non-inferiority randomized study.

The clinical interest of using bubble humidification of oxygen remains controversial. This study was designed to further explore whether delivering dry oxygen instead of bubble-moistened oxygen had an impact on discomfort of ICU patients. This randomized multicenter non-inferiority open trial included patients admitted in intensive care unit and receiving oxygen. Any patient receiving non-humidified oxygen (between 0 and 15 L/min) for less than 2 h could participate in the study. Randomization was stratified based on the flow rate at inclusion (less or more than 4 L/min). Discomfort was assessed 6-8 and 24 h after inclusion using a dedicated 15-item scale (quoted from 0 to 150). Three hundred and fifty-four ICU patients receiving non-humidified oxygen were randomized either in the humidified (HO) (n = 172), using bubble humidifiers, or in the non-humidified (NHO) (n = 182) arms. In modified intention-to-treat analysis at H6-H8, the 15-item score was 26.6 ± 19.4 and 29.8 ± 23.4 in the HO and NHO groups, respectively. The absolute difference between scores in both groups was 3.2 [90% CI 0.0; + 6.5] for a non-inferiority margin of 5.3, meaning that the non-inferiority analysis was not conclusive. This was also true for the subgroups of patients receiving either less or more than 4 L/min of oxygen. At H24, using NHO was not inferior compared to HO in the general population and in the subgroup of patients receiving 4 L/min or less of oxygen. However, for patients receiving more than 4 L/min, a post hoc superiority analysis suggested that patients receiving dry oxygen were less comfortable. Oxygen therapy-related discomfort was low. Dry oxygen could not be demonstrated as non-inferior compared to bubble-moistened oxygen after 6-8 h of oxygen administration. At 24 h, dry oxygen was non-inferior compared to bubble-humidified oxygen for flows below 4 L/min

The Effectiveness of Support and Rehabilitation Services for Women Offenders

There is a large body of research evidence suggesting that support, rehabilitation, and supervision programs can help offenders to reduce recidivism. However, the effectiveness of these services is dependent upon the extent to which the workers who deliver them comply with "what works" principles and practices. Because most of this research has been conducted with men, this study focused on the extent to which these principles and practices apply to women. In particular, the study examined services offered to a group of women in prison in Victoria, Australia, and following their release to the community; and the relationship between these women's views about the services, recidivism, and the characteristics of the services. Results were generally consistent with earlier research. The women favoured services that are delivered by workers who are reliable, holistic, collaborative, who understand the women's perspective, and that focus on strengths. They did not support services that challenged the women, focused on their offences, or on the things they did badly

Antiparasite treatments reduce humoral immunity and impact oxidative status in raptor nestlings

Parasites are natural stressors that may have multiple negative effects on their hos as they usurp energy and nutrients and may lead to costly immune responses that may cause oxidative stress. At early stages, animals may be more sensitive to infectious organisms because of their rapid growth and partly immature immune system. The objective of this study was to explore effects of parasites by treating chicks of two raptor species (northern goshawk Accipiter gentilis and white‐tailed sea eagle Haliaeetus albicilla) against both endoparasites (internal parasites) and ectoparasites (external parasites). Nests were either treated against ectoparasites by spraying with pyrethrin or left unsprayed as control nests. Within each nest, chicks were randomly orally treated with either an antihelminthic medication (fenbendazole) or sterile water as control treatment. We investigated treatment effects on plasma (1) total antioxidant capacity TAC (an index of nonenzymatic circulating antioxidant defenses), (2) total oxidant status TOS (a measure of plasmatic oxidants), and (3) immunoglobulin levels (a measure of humoral immune function). Treatment against ectoparasites led to a reduction in circulating immunoglobulin plasma levels in male chicks. TOS was higher when not receiving any parasite reduction treatment and when receiving both endo‐ and ectoparasitic reduction treatment compared with receiving only one treatment. TAC was higher in all treatment groups, when compared to controls. Despite the relatively low sample size, this experimental study suggests complex but similar relationships between treatment groups and oxidative status and immunoglobulin levels in two raptor species

β-Microseminoprotein Endows Post Coital Seminal Plasma with Potent Candidacidal Activity by a Calcium- and pH-Dependent Mechanism

The innate immune factors controlling Candida albicans are mostly unknown. Vulvovaginal candidiasis is common in women and affects approximately 70–75% of all women at least once. Despite the propensity of Candida to colonize the vagina, transmission of Candida albicans following sexual intercourse is very rare. This prompted us to investigate whether the post coital vaginal milieu contained factors active against C. albicans. By CFU assays, we found prominent candidacidal activity of post coital seminal plasma at both neutral and the acid vaginal pH. In contrast, normal seminal plasma did not display candidacidal activity prior to acidification. By antifungal gel overlay assay, one clearing zone corresponding to a protein band was found in both post coital and normal seminal plasma, which was subsequently identified as β-microseminoprotein. At neutral pH, the fungicidal activity of β-microseminoprotein and seminal plasma was inhibited by calcium. By NMR spectroscopy, amino acid residue E71 was shown to be critical for the calcium coordination. The acidic vaginal milieu unleashed the fungicidal activity by decreasing the inhibitory effect of calcium. The candidacidal activity of β-microseminoprotein was mapped to a fragment of the C-terminal domain with no structural similarity to other known proteins. A homologous fragment from porcine β-microseminoprotein demonstrated calcium-dependent fungicidal activity in a CFU assay, suggesting this may be a common feature for members of the β-microseminoprotein family. By electron microscopy, β-microseminoprotein was found to cause lysis of Candida. Liposome experiments demonstrated that β-microseminoprotein was active towards ergosterol-containing liposomes that mimic fungal membranes, offering an explanation for the selectivity against fungi. These data identify β-microseminoprotein as an important innate immune factor active against C. albicans and may help explain the low sexual transmission rate of Candida

No selection on immunological markers in response to a highly virulent pathogen in an Arctic breeding bird

In natural populations, epidemics provide opportunities to look for intense natural selection on genes coding for life history and immune or other physiological traits. If the populations being considered are of management or conservation concern, then identifying the traits under selection (or 'markers') might provide insights into possible intervention strategies during epidemics. We assessed potential for selection on multiple immune and life history traits of Arctic breeding common eiders (Somateria mollissima) during annual avian cholera outbreaks (summers of 2006, 2007 & 2008). We measured prelaying body condition, immune traits, and subsequent reproductive investment (i.e., clutch size) and survival of female common eiders and whether they were infected with Pasteurella multocida, the causative agent of avian cholera. We found no clear and consistent evidence of directional selection on immune traits; however, infected birds had higher levels of haptoglobin than uninfected birds. Also, females that laid larger clutches had slightly lower immune responses during the prelaying period reflecting possible downregulation of the immune system to support higher costs of reproduction. This supports a recent study indicating that birds investing in larger clutches were more likely to die from avian cholera and points to a possible management option to maximize female survival during outbreaks

HIV-1 Enhancing Effect of Prostatic Acid Phosphatase Peptides Is Reduced in Human Seminal Plasma

We recently reported that HIV-1 infection can be inhibited by innate antimicrobial components of human seminal plasma (SP). Conversely, naturally occurring peptidic fragments from the SP-derived prostatic acid phosphatase (PAP) have been reported to form amyloid fibrils called “SEVI” and enhance HIV-1 infection in vitro. In order to understand the biological consequence of this proviral effect, we extended these studies in the presence of human SP. PAP-derived peptides were agitated to form SEVI and incubated in the presence or absence of SP. While PAP-derived peptides and SEVI alone were proviral, the presence of 1% SP ablated their proviral activity in several different anti-HIV-1 assays. The anti-HIV-1 activity of SP was concentration dependent and was reduced following filtration. Supraphysiological concentrations of PAP peptides and SEVI incubated with diluted SP were degraded within hours, with SP exhibiting proteolytic activity at dilutions as high as 1∶200. Sub-physiological concentrations of two prominent proteases of SP, prostate-specific antigen (PSA) and matriptase, could degrade physiological and supraphysiological concentrations of PAP peptides and SEVI. While human SP is a complex biological fluid, containing both antiviral and proviral factors, our results suggest that PAP peptides and SEVI may be subject to naturally occurring proteolytic components capable of reducing their proviral activity

L'HYDROPTERE: HOW MULTIDISCIPLINARY SCIENTIFIC RESEARCH MAY HELP BREAK THE SAILING SPEED RECORD

In 2009, l’Hydroptère broke the symbolic barrier of 50 knots and became the world fastest sailing boat over both 500 meters and 1 nautical mile. This major achievement relied on the high skills of the sailing team but also on technical advances of the boat, resulting from the scientific collaboration between the Hydroptère Design Team and the Ecole Polytechnique Fédérale de Lausanne (EPFL). In the present article, we highlight the multidisciplinary research activity performed within EPFL in the course of this collaboration involving aero- and hydrodynamics, materials and structure as well as computer vision. Various foils were tested at reduced scale in a high speed water tunnel, and the results used to validate the numerical simulations. Composite materials, their processing parameters and assembly components were tested. The structural behaviour was also investigated to determine strains and stresses in normal and extreme sailing conditions, taking waves into account, and a combined model was derived for dynamic simulation. Finally, advanced computer vision methods were developed and implemented on the boat to monitor foil immersion and cross beams deformations