Effects of acute caffeine ingestion on futsal performance in sub-elite players.

Purpose To date, no previous investigation has studied the effect of acute caffeine ingestion on futsal performance during futsal-specific testing and during a simulated match. Therefore, the aim of this investigation was to establish the effects of acute caffeine intake on futsal-specific tests and match-play running performance in male futsal players. Methods Sixteen high-performance futsal players participated in a randomized, crossover, placebo-controlled and double-blind experiment. Each player completed two identical trials after ingesting either caffeine (3 mg/kg) or a placebo (cellulose). The trials consisted of a battery of futsal-specific tests (countermovement jump, 20-m sprint test, and a futsal kicking velocity and accuracy test) followed by a simulated futsal match (2 halves of 7.5 min). During the match, players’ running performance was assessed with local positioning system devices. Results In comparison to the placebo, caffeine ingestion increased jump height by 2.8% (p = 0.048; ES = 0.29) and reduced the time to complete the 20-m sprint test by -2.2% (p = 0.044; ES = − 0.54). Additionally, acute caffeine intake improved the distance covered at above 14.4 km/h by 19.6% (p = 0.021; ES = 0.58), the number of body impacts by 8.1% (p = 0.040; ES = 0.27) and the number of accelerations/decelerations by 4.2% (p = 0.044; ES = 0.57) during the simulated futsal match. However, no differences were reported in ball velocity or shooting accuracy in the futsal kicking test. There were no differences in the prevalence of side effects reported in the hours after the ingestion of the treatments. Conclusion Three mg/kg of caffeine enhanced several physical variables associated with futsal such as jump and sprint performance, and improved high-speed running and accelerations/decelerations during a simulated futsal match. Caffeine supplementation with a moderate dose can be considered as an effective ergogenic aid for futsal performance with low prevalence of side effects.pre-print920 K

Laboratory and telescope demonstration of the TP3-WFS for the adaptive optics segment of AOLI

AOLI (Adaptive Optics Lucky Imager) is a state-of-art instrument that combines adaptive optics (AO) and lucky imaging (LI) with the objective of obtaining diffraction limited images in visible wavelength at mid- and big-size ground-based telescopes. The key innovation of AOLI is the development and use of the new TP3-WFS (Two Pupil Plane PositionsWavefront Sensor). The TP3-WFS, working in visible band, represents an advance over classical wavefront sensors such as the Shack-Hartmann WFS (SH-WFS) because it can theoretically use fainter natural reference stars, which would ultimately provide better sky coverages to AO instruments using this newer sensor. This paper describes the software, algorithms and procedures that enabled AOLI to become the first astronomical instrument performing real-time adaptive optics corrections in a telescope with this new type of WFS, including the first control-related results at the William Herschel Telescope (WHT)This work was supported by the Spanish Ministry of Economy under the projects AYA2011-29024, ESP2014-56869-C2-2-P, ESP2015-69020-C2-2-R and DPI2015-66458-C2-2-R, by project 15345/PI/10 from the Fundación Séneca, by the Spanish Ministry of Education under the grant FPU12/05573, by project ST/K002368/1 from the Science and Technology Facilities Council and by ERDF funds from the European Commission. The results presented in this paper are based on observations made with the William Herschel Telescope operated on the island of La Palma by the Isaac Newton Group in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias. Special thanks go to Lara Monteagudo and Marcos Pellejero for their timely contributions

Edema Agudo de Pulmón Post-Cesárea

Presentamos un caso clínico de hipoxemia secundaria a Edema Agudo de Pulmón (EAP) en el postoperatorio inmediato de una cesárea bajo anestesia general. En este documento, revisamos el proceso de diagnóstico diferencial aplicado a nuestra paciente

Near real time pump optimization and pressure management

[EN] Management of existing systems can be interpreted as sets of decisions to make regarding pumps and valves to create hydraulic conditions able to satisfy the demand without operational problems such as pressures lower or higher than the normative pressure values. However, among the large number of combinations, some of them manage to reduce energy consumption, by finding the best operating point for pumps, and also water losses, by finding the best operating point for pressure reducing valves (PRV). Several works may be found in the literature using recent and advanced optimization techniques to define pump and valve operation. However, the processing time to define operational rules is a limiting factor for real time decision-making. Taking into account the need to improve the models in terms of optimal rules to apply in near real-time operations, this work presents a hybrid model (simulator + optimizer) to find pump speeds and PRV set points, aiming at combining energy savings with pressure control while reducing water losses. PSO is applied as the main optimization algorithm, which can also work in cooperation with other bio-inspired concepts to deploy an effective and fast search algorithm. The results allow comparisons with other techniques and show the ability of PSO to find an optimal point of operationBrentan, BM.; Luvizotto, EJ.; Montalvo, I.; Izquierdo Sebastián, J.; Pérez García, R. (2017). Near real time pump optimization and pressure management. Procedia Engineering. 186:666-675. doi:10.1016/j.proeng.2017.06.248S66667518

Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise

Interactions between co-existing microorganisms deeply affect the physiology of the involved organisms and, ultimately, the function of the ecosystem as a whole. Copiotrophic Alteromonas are marine gammaproteobacteria that thrive during the late stages of phytoplankton blooms in the marine environment and in laboratory co-cultures with cyanobacteria such as Trichodesmium. The response of this heterotroph to the sometimes rapid and transient changes in nutrient supply when the phototroph crashes is not well understood. Here, we isolated and sequenced the strain Alteromonas macleodii str. Te101 from a laboratory culture of Trichodesmium erythraeum IMS101, yielding a chromosome of 4.63 Mb and a single plasmid of 237 kb. Increasing salinities to ≥43 ppt inhibited the growth of Trichodesmium but stimulated growth of the associated Alteromonas. We characterized the transcriptomic responses of both microorganisms and identified the complement of active transcriptional start sites in Alteromonas at single-nucleotide resolution. In replicate cultures, a similar set of genes became activated in Alteromonas when growth rates of Trichodesmium declined and mortality was high. The parallel activation of fliA, rpoS and of flagellar assembly and growth-related genes indicated that Alteromonas might have increased cell motility, growth, and multiple biosynthetic activities. Genes with the highest expression in the data set were three small RNAs (Aln1a-c) that were identified as analogs of the small RNAs CsrB-C in E. coli or RsmX-Z in pathogenic bacteria. Together with the carbon storage protein A (CsrA) homolog Te101_05290, these RNAs likely control the expression of numerous genes in responding to changes in the environmentThis work was supported by the EU project MaCuMBA (grant agreement no. 311975) to WRH and FRVby the German Israeli Foundation grant no. 1133 to IB-F and WRHSupport of ML-P by a postdoctoral fellowship from the Valencian Consellería de Educació, Investigació, Cultura i Esport (APOSTD/2016/051)of SH by the China Scholarship Council is gratefully acknowledged

Deficient Spindle Assembly Checkpoint in Multiple Myeloma

Multiple myeloma (MM) is a hematological disease characterized by an abnormal accumulation of plasma cells in the bone marrow. These cells have frequent cytogenetic abnormalities including translocations of the immunoglobulin heavy chain gene and chromosomal gains and losses. In fact, a singular characteristic differentiating MM from other hematological malignancies is the presence of a high degree of aneuploidies. As chromosomal abnormalities can be generated by alterations in the spindle assembly checkpoint (SAC), the functionality of such checkpoint was tested in MM. When SAC components were analyzed in MM cell lines, the RNA levels of most of them were conserved. Nevertheless, the protein content of some key constituents was very low in several cell lines, as was the case of MAD2 or CDC20 in RPMI-8226 or RPMI-LR5 cells. The recovery of their cellular content did not substantially affect cell growth, but improved their ability to segregate chromosomes. Finally, SAC functionality was tested by challenging cells with agents disrupting microtubule dynamics. Most of the cell lines analyzed exhibited functional defects in this checkpoint. Based on the data obtained, alterations both in SAC components and their functionality have been detected in MM, pointing to this pathway as a potential target in MM treatment

Study on Viability and Chondrogenic Differentiation of Cryopreserved Adipose Tissue-Derived Mesenchymal stromal Cells for future use in regenerative medicine

P. 256–263Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defective tissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinical applications, adipose-derived stem cell cryopreservation is an essential fundamental technology. The aim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymal stromal cells, by comparing various protocols so as to determine the effects of cryopreservation on viability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing of AT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocal microscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-term cryopreserved without any loss of their proliferative or differentiation potentialS

The prognostic value of multiparameter flow cytometry minimal residual disease assessment in relapsed multiple myeloma

Letter to the editor.-- et al.This study was supported by the Cooperative Research Thematic Network grants RD12/0036/0058 of the Red de Cancer (Cancer Network of Excellence); Instituto de Salud Carlos III, Spain, Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS: PI060339; 06/1354; 02/0905; 01/0089/01-02; PS09/01897/01370; G03/136; Sara Borrell: CD13/00340); and Asociación Española Contra el Cáncer (GCB120981SAN), Spain. The study was also supported internationally by the International Myeloma Foundation Junior Grant Proposal and the Multiple Myeloma Research Foundation research fellow award.Peer Reviewe