Influence of 1-Methylcyclopropene Treatment on Postharvest Quality of Four Scab (Venturia inaequalis)-Resistant Apple Cultivars

Scab (Venturia inaequalis) is a very serious disease for apples causing up to 80% of loss in yield but there are only a few studies on postharvest quality of scab-resistant cultivars. In this study we evaluated the effect of 1-methylcyclopropene (1-MCP) on fruit quality, total phenolic content, and antioxidant capacity after storage of four scab-resistant cultivars and compared to a standard cultivar, “Golden Delicious.” In general, ethylene production and respiration rates significantly differed among cultivars, between control and 1-MCP-treated fruits, and between storage duration regimes. 1-MCP treatment retarded fruit softening and lowered juice pH but storage effect on soluble solids and acidity depended on cultivar and 1-MCP treatment. Total phenolic content was significantly affected by storage duration and 1-MCP treatment. Antioxidant capacity of the four scab-resistant cultivars was either similar to or significantly higher than that of “Golden Delicious” with the 1-MCP-treated fruits having significantly higher antioxidant capacity than the nontreated fruits after storage. Our results clearly show that the quality of four scab-resistant cultivars was comparable to that of “Golden Delicious” and 1-MCP effect differed among cultivars. These differences need to be considered in developing storage regime to minimize quality deterioration during long-term storage

Influence of 1-Methylcyclopropene Treatment on Postharvest Quality of Four Scab ( Venturia inaequalis

Scab (Venturia inaequalis) is a very serious disease for apples causing up to 80% of loss in yield but there are only a few studies on postharvest quality of scab-resistant cultivars. In this study we evaluated the effect of 1-methylcyclopropene (1-MCP) on fruit quality, total phenolic content, and antioxidant capacity after storage of four scab-resistant cultivars and compared to a standard cultivar, “Golden Delicious.” In general, ethylene production and respiration rates significantly differed among cultivars, between control and 1-MCP-treated fruits, and between storage duration regimes. 1-MCP treatment retarded fruit softening and lowered juice pH but storage effect on soluble solids and acidity depended on cultivar and 1-MCP treatment. Total phenolic content was significantly affected by storage duration and 1-MCP treatment. Antioxidant capacity of the four scab-resistant cultivars was either similar to or significantly higher than that of “Golden Delicious” with the 1-MCP-treated fruits having significantly higher antioxidant capacity than the nontreated fruits after storage. Our results clearly show that the quality of four scab-resistant cultivars was comparable to that of “Golden Delicious” and 1-MCP effect differed among cultivars. These differences need to be considered in developing storage regime to minimize quality deterioration during long-term storage

Sulforaphane Induces Antioxidative and Antiproliferative Responses by Generating Reactive Oxygen Species in Human Bronchial Epithelial BEAS-2B Cells

Sulforaphane (SFN) is a naturally occurring compound which is known to induce the phase II antioxidant genes via Nrf2 activation, although the underlying mechanism has not been fully elucidated. In this study, we investigated Nrf2 induction in response to SFN in human bronchial epithelial BEAS-2B cells and determined the signaling pathways involved in this process. SFN treatment reduced cell viability. Prior to cell death, intracellular reactive oxygen species (ROS) were generated at a high rate within a minute of commencing SFN treatment. Pretreatment with antioxidant N-acetylcysteine (NAC) blocked SFN-induced decrease in cell growth. Erk1/2 was activated within 30 min of SFN addition, whereas Akt phosphorylation did not significantly change until the first 8 hr after SFN treatment but then became substantially low until 48 hr. Inhibition of Erk1/2 phosphorylation attenuated SFN-induced loss of cell viability. Nrf2 protein levels in both nuclear and whole cell lysates were increased by SFN treatment, which was dependent on ROS production. Knockdown of Nrf2 with siRNA attenuated SFN-induced heme oxygenase-1 (HO-1) up-regulation. Induction of the Nrf2/HO-1 after SFN treatment was potently suppressed by pretreatment with NAC. Overall, our results indicate that SFN mediates antioxidative and antiproliferative responses by generating ROS in BEAS-2B cells

An Integrated Apprch for Enhancing Food Safety in Qatar

This study was carried out to assess hygiene conditions, food handling practices, food safety knowledge of food service providers (FSPs), and the microbial quality of food served in different food service establishments in Doha. Fifty-three FSPs were randomly selected among 200 FSPs. Face-to-face interviews with the food safety managers at each participating FSP were conducted using a survey consisting of 40-questions (demographic data on workers, HACCP training, knowledge on personal hygiene, and safe-food handling practices) in October-December 2015. In addition to survey questionnaire, a checklist was used to determine the implementation of international food safety standards by observing actual practices applied at each FSP. All FSPs who took part in the initial survey were also invited to participate in one of three one-day educational workshops focused mainly on important components of food safety management system and held in 2016. At the time of workshops, each participant was also encouraged to take part in the microbial quality assessment study. Out of 53 establishments, only 10 FSPs (2 fine-dine-in, 2 casual sit-in, 2 catering, 2 fast-food, and 2 takeaway) accepted to provide food and swab samples from their entities. At the time of each visit to select FSPs, various menu items (food cooked in a short time, ready-to-eat foods, vegetables, dairy-based deserts, sandwiches, and raw seafood, e.g. oysters) were sampled in duplicate (based on the daily menu prepared at the time of sampling) at different food preparation stages (receiving, food storage, food preparation, holding/cooking, and serving). The microbial quality of food samples (n = 105) served and swabs collected from food preparation surfaces (n = 58) were also assessed using select media (APC, MCA, XLT4, and LSA). The identification of positive samples was carried out using VITEK-2 system. After the microbial assessment, a follow-up survey consisting of 24 questions was developed to determine the impact of the educational trainings on food service providers' daily operational practices. The FSP managers who participated in the initial survey were invited by phone or email or in person to take part in the follow-up survey. Out of 53 FSPs, only 16 were available to answer the questions due to reasons beyond the control of the research team. The major reasons for a low participation were 1) several managers moved back to their country without any contact information, 2) many of them changed their jobs and there was a no way of communicating with them since they did not provide an alternative email or a phone number. The survey results indicated that average service years of FSPs was 11, the average age of food safety managers interviewed was 33, most managers (66%) had college degree, and 68% of them were trained on HACCP. It was demonstrated that casual-sit-in and fine-dine-in restaurants are the only FSP types which consistently kept records (100%), followed by fast-food (36%), and catering (14%) FSPs. The microbial analysis indicated that the average APC in food samples collected from all FSPs met the international standards, while the APC counts of swab samples were considered unsatisfactory since the levels were above 106 Log10 CFU/cm2. The highest bacterial count was reported in swab samples (7.26 Log10 CFU/cm2) collected from preparation area in takeaway restaurants. Concerning the target organisms (Escherichia coli, Salmonella spp., and Listeria monocytogenes), among 105 food samples and 58 swab samples collected, 13 samples (8%) exhibited positive results for possible target pathogens. Positive samples were identified as Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, and Pantoea spp. Overall, the participants were highly satisfied (average score: 4.39/5 ± 0.20) with the information presented in the training workshops held in 2016, indicating that the workshops helped improve their knowledge on food safety; change their attitudes towards safe handling foods, and inform them about the changes on food safety laws and regulations in Qatar. These results provided insight information on the aspects of behavioural changes that confirm the value of intervention studies. One important note which is important to mention that the participating managers indicated that they need open communication between the policymakers and the FSPs to be able to make sure that they will not be left behind if there is any changes/updates on food safety rules and regulations applied in the country. This issue is going to be addressed by posting constant updates on GSO (food safety standards applied in Qatar) on the website recently developed and hosted under the Ministry of Public Health website. Results obtained in this study might help food safety managers in these select FSPs to better understand the need for implementing effective control measures in order to prevent contamination and eventually protect the public health.qscienc

Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol

Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5–32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g−1 fresh weight, respectively

Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines

none7noCruciferous vegetables are an important source of compounds that may be useful for chemoprevention. In this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of Brassica oleracea on both estrogen receptor (ER)-positive (ER; MCF-7 and BT474) and ER-negative (ER; MDA-MB-231 and BT20) human breast cancer cell lines. The effect of juice on cell proliferation was evaluated on DNA synthesis and on cell cycle–related proteins. Juice markedly reduced DNA synthesis, evaluated by [3H]thymidine incorporation, starting from low concentrations (final concentration 5–15 mL/L), and this activity was independent of ER. All cauliflower varieties tested suppressed cell proliferation in a dose-dependent manner. Cell growth inhibition was accompanied by significant cell death at the higher juice concentrations, although no evidence of apoptosis was found. Interestingly, the juice displayed a preferential activity against breast cancer cells compared with other mammalian cell lines investigated (ECV304, VERO, Hep2, 3T3, and MCF-10A) (P 0.01). At the molecular level, the inhibition of proliferation was associated with significantly reduced CDK6 expression and an increased level of p27 in ER cells but not in ER cells, whereas a common feature in all cell lines was significantly decreased retinoblastoma protein phosphorylation. These results suggest that the edible part of Brassica oleracea contains substances that can markedly inhibit the growth of both ER and ER human breast cancer cells, although through different mechanisms. These results suggest that the widely available cruciferous vegetables are potential chemopreventive agents. JopenBrandi, Giorgio; Schiavano, GIUDITTA FIORELLA; Zaffaroni, N; De Marco, C; Paiardini, M; Cervasi, B; Magnani, MauroBrandi, G; Schiavano, Gf; Zaffaroni, N; De Marco, C; Paiardini, M; Cervasi, B; Magnani, M

Comparison of the effect of raw and blanched-frozen broccoli on DNA damage in colonocytes

Consumption of cruciferous vegetables may protect against colorectal cancer. Cruciferous vegetables are rich in a number of bioactive constituents including polyphenols, vitamins and glucosinolates. Before consumption, cruciferous vegetables often undergo some form of processing that reduces their content of bioactive constituents and may determine whether they exert protective effects. The aim of this study was to compare the ability of raw and blanched-frozen broccoli to protect colonocytes against DNA damage, improve antioxidant status and induce xenobiotic metabolising enzymes (XME). Fifteen Landrace x Large White male pigs were divided into five age and weight matched sets (79 days, SD 3, and 34.7 kg, SD 3.9 respectively). Each set consisted of siblings to minimise genetic variation. Within each set, pigs received a cereal-based diet, unsupplemented (control) or supplemented with 600 g/d of raw or blanched-frozen broccoli for 12 d. The consumption of raw broccoli caused a significant 27% increase in DNA damage in colonocytes (P=0.03) relative to the control diet, whereas blanched-frozen broccoli had no significant effect. Both broccoli diets had no significant effect on plasma antioxidant status or hepatic and colonic XME. This study is the first to report that the consumption of raw broccoli can damage DNA in porcine colonocytes

Tri-trophic effects of inter- and intra-population variation in defence chemistry of wild cabbage (Brassica oleracea)

The effect of direct chemical defences in plants on the performance of insect herbivores and their natural enemies has received increasing attention over the past 10 years. However, much less is known about the scale at which this variation is generated and maintained, both within and across populations of the same plant species. This study compares growth and development of the large cabbage butterfly, Pieris brassicae, and its gregarious pupal parasitoid, Pteromalus puparum, on three wild populations [Kimmeridge (KIM), Old Harry (OH) and Winspit (WIN)] and two cultivars [Stonehead (ST), and Cyrus (CYR)] of cabbage, Brassica oleracea. The wild populations originate from the coast of Dorset, UK, but grow in close proximity with one another. Insect performance and chemical profiles were made from every plant used in the experiment. Foliar glucosinolates (GS) concentrations were highest in the wild plants in rank order WIN > OH > KIM, with lower levels found in the cultivars. Caterpillar-damaged leaves in the wild cabbages also had higher GS levels than undamaged leaves. Pupal mass in P. brassicae varied significantly among populations of B. oleracea. Moreover, development time in the host and parasitoid were correlated, even though these stages are temporally separated. Parasitoid adult dry mass closely approximated the development of its host. Multivariate statistics revealed a correlation between pupal mass and development time of P. brassicae and foliar GS chemistry, of which levels of neoglucobrassicin appeared to be the most important. Our results show that there is considerable variation in quantitative aspects of defensive chemistry in wild cabbage plants that is maintained at very small spatial scales in nature. Moreover, the performance of the herbivore and its parasitoid were both affected by differences in plant quality