Dental zirconia: in-vitro comparison and outcome of methods for veneering, glazing, and chipping repairs

OBJECTIVE: The purpose of this study is to evaluate the experimental properties of zirconia-based ceramics. METHODS: Zirconia bars were veneered to 2mm total thickness. Veneering-method groups included: 1.Hand-layered feldsparthic porcelain (VM=VitaVM9,Vident) and fluorapatite glass-ceramic (CR=IPSe.maxCeram,IvoclarVivadent); 2.Pressed feldspathic porcelain (PM=VitaPM9,Vident) and fluorapatite glass-ceramic (ZP=IPSe.maxZirPress,IvoclarVivadent); 3.CAD/CAM milled feldspathic ceramic (TF=VitablocsTriluxeForte,Vident) and lithium-disilicate glass-ceramic (CAD=IPSe.maxCAD,IvoclarVivadent). CAD/CAM veneers were either cemented with resin cements (P=Panavia21,KurarayDental), (R=RelyXUltimate,3M ESPE), (M=MultilinkAutomix,IvoclarVivadent) or fused with fusion glass-ceramic (C=CrystalConnect,IvoclarVivadent). A Three-point-bending test was performed. For group VM,PM,TF-M,TF-C,CAD-M,CAD-C, ten more bars were prepared and aged with cyclic loading and thermocycling before testing. Zirconia bars (PrettauZirconia,Zirkonzahn;inCorisTZI,Sirona;ZirluxFC,PentronCeramics) specimens were prepared and polished. The specimens were divided into 3 groups: control, self-glaze fired, and glazed groups. A Three-point bending test was performed. Veneered zirconia crowns were made. Feldspathic porcelain was applied to zirconia coping. Bevel cut on porcelain was made to simulate porcelain chipping. The crowns were then divided into 4 different groups according to repair materials including: 1.Conventional-resin composite (TetricEvoCeram,IvoclarVivadent) 2.Flowable-resin composite (G-aenialUniversalFlo,GCamerica) 3.Cemented CAD/CAM milled feldspathic ceramic (VitaTriluxForte,Vident) 4.Cemented CAD/CAM milled lithium-disilicate glass-ceramic (IPSe.maxCAD,IvoclarVivadent). Each crown underwent thermocycling. The test was performed by loading force on the center of repaired part to record load-to-failure. RESULTS: There were significant differences in the failure loads of non-aged bilayered veneer-zirconia bars. Aging experiment revealed a significant difference in failure load between non-aged and aged bars in groups VM and PM, but not in the groups with CAD/CAM milled veneers. There was significant influence of surface treatments on flexural strength of zirconia specimens. Post-hoc test showed that glazed group had significant lower flexural strength than other groups. Crowns repaired with CAD/CAM ceramics showed significant higher failure load than resin composite. Repairing with lithium-disilicate glass ceramic yielded the highest load-to-failure of the specimens. CONCLUSIONS: - Veneer materials, veneering methods, and cement materials have a significant effect on the failure load of bilayered veneer- zirconia. CAD/CAM veneer-zirconia is not susceptible to aging performed in this study. - Glazing decreased the flexural strength of high translucent zirconia. - Veneered zirconia crowns repaired with CAD/CAM ceramic materials have significantly higher load-to-failure than veneered crowns repaired with resin composite.2018-09-28T00:00:00

Isolation and Characterization of Probiotic Properties of Lactic Acid Bacteria Isolated from Native Chicken Manure

บทคัดย่อ การใช้ยาปฏิชีวนะสำหรับเลี้ยงไก่เพื่อกระตุ้นการเจริญเติบโตถูกนำมาใช้อย่างแพร่หลายอย่างต่อเนื่องในระยะยาว ส่งผลกระทบทางลบโดยทำให้เกิดแบคทีเรียดื้อยาปฏิชีวนะ ดังนั้นทางเลือกในการลดผลกระทบของแบคทีเรียดื้อยาปฏิชีวนะแต่ยังสามารถกระตุ้นการเจริญเติบโตของสัตว์พร้อมทั้งยังป้องกันโรคได้ คือ การใช้จุลินทรีย์โพรไบโอติกที่มีประสิทธิภาพต่อการป้องกันการก่อโรคในลำไส้ และช่วยส่งเสริมระบบย่อยอาหารของไก่ แบคทีเรียหลายชนิดมีคุณบัติเป็นโพรไบโอติก แต่แบคทีเรียกรดแลคติก (lactic acid bacteria) เป็นกลุ่มแบคทีเรียที่มีศักยภาพมากที่สุดสำหรับใช้เป็นโพรไบโอติกเสริมในอาหารสัตว์ ดังนั้นการศึกษานี้จึงมีวัตถุประสงค์เพื่อคัดแยกและศึกษาคุณสมบัติความเป็นโพรไบโอติกของแบคทีเรียกรดแลคติกที่แยกได้จากมูลไก่พันธุ์พื้นเมือง จากตัวอย่างมูลไก่พันธุ์พื้นเมือง 27 ตัวอย่าง สามารถแยกแบคทีเรียกรดแลคติกได้ 171 ไอโซเลท จากการศึกษาคุณสมบัติของโพรไบโอติก ได้แก่ ความสามารถต้านเชื้อแบคทีเรียก่อโรค ความสามารถทนต่อเกลือน้ำดี ความสามารถทนกรด และคุณสมบัติ hydrophobicity พบว่า แบคทีเรียกรดแลคติกจำนวน 6 ไอโซเลท จากทั้งหมด 171 มีคุณสมบัติเหมาะสมสำหรับเป็นโพรไบโอติก ผลการจัดจำแนกโดยวิเคราะห์ลำดับนิวคลีโอไทด์ของยีน 16S rRNA พบว่าทั้ง 6 ไอโซเลท เป็นเชื้อ Enterococcus faecium, Lactobacillus plantarum และ L. salivarius ผลการศึกษานี้สามารถแยกแบคทีเรียกรดแลคติกที่มีคุณสมบัติของโพรไบโอติกที่เหมาะสมต่อการพัฒนาเป็นโพรไบโอติกสำหรับสัตว์ปีกต่อไป คำสำคัญ : แบคทีเรียกรดแลคติก โพรไบโอติก ไก่พันธุ์พื้นเมือง  ABSTRACTThe long-term continuous using of antibiotics as a growth promoter in chicken has a negative impact on emerging of antibiotics resistance bacteria. Therefore, an alternative way to reduce the effects of antibiotics resistant bacteria, but still promote the growth of chicken together with an efficiently prevention of disease is using of an effective probiotics microorganisms. Many microorganisms have been using as probiotics, but lactic acid bacteria (LAB) seem to be the potential probiotic agent in chicken feed. Therefore, the objectives of this study are to isolate, identify and characterize the probiotic properties of LAB isolated from the native chicken manure. The LAB was isolated from the manure of 27 native chickens. This study found that 171 LAB were isolated from samples of native chicken manure. The probiotic properties, such as the antibacterial activity against pathogenic bacteria, bile salt tolerance, acid tolerance and hydrophobicity were determined in all the 171 isolates. The results showed that of total 171 isolates, 6 isolates that showed the satisfactory results of probiotic properties. The 16s rRNA gene sequencing analysis indicated that were Enterococcus faecium, Lactobacillus plantarum and L. salivarius. Throughout this study, we able to isolate 6 LAB from chicken manure and demonstrate their probiotic properties that suitable for further development of probiotics for poultry.Keywords: Lactic Acid Bacteria, Probiotics, Native Chicken Manur

Effect of Glazing on Flexural Strength of Full-Contour Zirconia

Objective. The purpose of this study was to evaluate the effect of glazing on flexural strength of highly translucent zirconia materials. Materials and Methods. Specimens of three brands of zirconia bars (Prettau Zirconia, Zirkonzahn; inCoris TZI, Sirona; and Zirlux FC, Pentron Ceramics) were prepared and polished according to manufacturers’ instructions. Final specimen dimensions were 20 × 4 × 2 mm. The specimens from each brand were divided into 3 groups (N = 10): control, heat-treated, and glazed. Heat-treated specimens were fired without the application of the glaze material. The glaze material was applied to the glazed specimens before being fired. A three-point bending test (15 mm span) was performed in an Instron universal testing machine (ISO 6872). Data were analyzed by ANOVA and Tukey’s HSD post hoc test (α = 0.05). Results. Two-way ANOVA showed a significant influence of surface treatments on flexural strength of zirconia materials (P≤0.05). There was no significant difference in flexural strength among the different brands of highly translucent zirconia (P≥0.05). Tukey’s HSD post hoc test showed that specimens in the “glazed” group had significantly lower flexural strength than the control and heat-treated groups (P≤0.05). Conclusion. Within the limitations of the study, external glazing decreased the flexural strength of highly translucent zirconia

Apresentando alguns aspectos históricos do desenvolvimento da lógica clássica, ciência das idéias e dos processos da mente

Lógica é a ciência que tem por objeto determinar, entre as operações intelectuais orientadas para o conhecimento da verdade, as que são válidas e as que não são. Estuda os processos e as condições de verdade de todo e qualquer raciocínio. O conhecimento só é científico quando, além de universal, é metódico e sistemático, ou seja, lógico. Assim, a lógica se entende como método, ou caminho que as ciências trilham para determinar e conhecer seu objeto, e como característica geral do conhecimento científico

Improving seed germination of the eggplant rootstock Solanum torvum by testing multiple factors using an orthogonal array design

[EN] Solanum torvum is a highly vigorous relative of eggplant that is resistant to a number of harmful soil-borne diseases and is compatible for grafting with eggplant. Being a potential rootstock, this plant frequently presents poor and erratic germination, which makes its practical use difficult. We used an L8 (2(7)) orthogonal array design to evaluate the primary effects of seven factors (soaking of seeds, scarification with sodium hypochlorite (NaClO), application of gibberellic acid (GA(3)), use of potassium mitrate (KNO3) as a moistening agent, cold stratification, application of a heatshock, and light irradiation during germination) at two levels (L0 and L1) using four germination parameters (early and final germination, germination rate and vigour index) in fresh S. torvum seeds. S. torvum seeds had a strong dormancy with no germination in the untreated seeds and high early and final germination (approximately 100%) in certain treatments. An evaluation of the main effects revealed highly positive effects on germination from seed soaking, and the use of GA(3), KNO3, and light irradiation, whereas NaClO scarification had a negative effect. The application of cold stratification and heat shock treatments also had a positive effect on seed germination but to a lesser extent than the other treatments. An improved proposed protocol that consisted of subjecting seeds to soaking, the application of GA(3) and KNO3, cold stratification, heat shock, and light irradiation was validated and demonstrated to be highly effective, with seed germination success greater than 60% being observed at 3 days and final germination reaching a plateau at 6 days. A second validation experiment using a commercial growing substrate also showed a high emergence (approximately 50%) at 7 days and a final germination of approximately 80% was recorded with application of the improved protocol. The seed germination protocol that we have developed will facilitate the use of S. torvum as a rootstock for eggplant and its use in breeding programmes. Our results also reveal that orthogonal array designs are a powerful tool for establishing improved protocols for seed germination. (C) 2015 Elsevier B.V. All rights reserved.This work was completed as part of the initiative "Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives", which is supported by the Government of Norway. The project is managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew and is implemented in partnership with national and international gene banks and plant breeding institutes. For further information see the project website: http://www.cwrdiversity.org/. Isabel Andujar and Pietro Gramazio are grateful to Universitat Politecnica de Valencia for their post-doctoral (PAID-10-14) and pre-doctoral (Programa FPI de la UPV-Subprograma 1) contracts, respectively.Ranil, RH.; Niran, HML.; Plazas Ávila, MDLO.; Fonseka, R.; Hemal Fonseka, H.; Vilanova Navarro, S.; Andújar Pérez, I.... (2015). Improving seed germination of the eggplant rootstock Solanum torvum by testing multiple factors using an orthogonal array design. Scientia Horticulturae. 193:174-181. https://doi.org/10.1016/j.scienta.2015.07.030S17418119

Crop wild relatives of the brinjal eggplant ( Solanum melongena ): Poorly represented in genebanks and many species at risk of extinction

This is the publisher's Early View pdf without final pagination .© 2016 Botanical Society of America. This is an open access article, available to all readers online, published under a creative commons license. The attached file is the published version of the article

Coding SNPs analysis highlights genetic relationships and evolution pattern in eggplant complexes

[EN] Brinjal (Solanum melongena), scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants are three Old World domesticates. The genomic DNA of a collection of accessions belonging to the three cultivated species, along with a representation of various wild relatives, was characterized for the presence of single nucleotide polymorphisms (SNPs) using a genotype-by-sequencing approach. A total of 210 million useful reads were produced and were successfully aligned to the reference eggplant genome sequence. Out of the 75,399 polymorphic sites identified among the 76 entries in study, 12,859 were associated with coding sequence. A genetic relationships analysis, supported by the output of the FastSTRUCTURE software, identified four major sub-groups as present in the germplasm panel. The first of these clustered S. aethiopicum with its wild ancestor S. anguivi; the second, S. melongena, its wild progenitor S. insanum, and its relatives S. incanum, S. lichtensteinii and S. linneanum; the third, S. macrocarpon and its wild ancestor S. dasyphyllum; and the fourth, the New World species S. sisymbriifolium, S. torvum and S. elaeagnifolium. By applying a hierarchical FastSTRUCTURE analysis on partitioned data, it was also possible to resolve the ambiguous membership of the accessions of S. campylacanthum, S. violaceum, S. lidii, S. vespertilio and S. tomentsum, as well as to genetically differentiate the three species of New World Origin. A principal coordinates analysis performed both on the entire germplasm panel and also separately on the entries belonging to sub-groups revealed a clear separation among species, although not between each of the domesticates and their respective wild ancestors. There was no clear differentiation between either distinct cultivar groups or different geographical provenance. Adopting various approaches to analyze SNP variation provided support for interpretation of results. The genotyping-by-sequencing approach showed to be highly efficient for both quantifying genetic diversity and establishing genetic relationships among and within cultivated eggplants and their wild relatives. The relevance of these results to the evolution of eggplants, as well as to their genetic improvement, is discussed.This work has been funded in part by European Unions Horizon 2020 Research and Innovation Programme under grant agreement No 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops) and by Spanish Ministerio de Economia, Industria y Competitividad and Fondo Europeo de Desarrollo Regional (grant AGL2015-64755-R from MINECO/FEDER). Funding has also been received from the initiative "Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives", which is supported by the Government of Norway. This last project is managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew and implemented in partnership with national and international gene banks and plant breeding institutes around the world. For further information see the project website:http://www.cwrdiversity.org/. Pietro Gramazio is grateful to Universitat Politecnica de Valencia for a pre-doctoral (Programa FPI de la UPV-Subprograma 1/2013 call) contract. Mariola Plazas is grateful to Spanish Ministerio de Economia, Industria y Competitividad for a post-doctoral grant within the Santiago Grisolia Programme (FCJI-2015-24835). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Acquadro, A.; Barchi, L.; Gramazio, P.; Portis, E.; Vilanova Navarro, S.; Comino, C.; Plazas Ávila, MDLO.... (2017). Coding SNPs analysis highlights genetic relationships and evolution pattern in eggplant complexes. PLoS ONE. 12(7). https://doi.org/10.1371/journal.pone.0180774Se018077412

Effects of Veneering Ceramic and Methods on Failure Load of Veneered Zirconia

Background: A variety of veneering options to zirconia frameworks are now available. The purpose of this study is to evaluate the effect of veneer materials, veneering methods, cement materials, and aging on the failure load of bilayered veneer zirconia. Material and methods: Zirconia bars (20 × 4 × 1 mm) were veneered to 2 mm total thickness (n = 10/group). Veneering method groups included: 1. Hand-layered feldsparthic porcelain (VM = Vita VM9, Vident) and fluorapatite glass–ceramic (CR = IPS e.max Ceram, IvoclarVivadent); 2. Pressed feldspathic porcelain (PM = Vita PM9, Vident) and fluorapatite glass–ceramic (ZP = IPS e.max ZirPress, IvoclarVivadent); 3. CAD-/CAM-milled feldspathic ceramic (TF = Vitablocs Triluxe Forte, Vident) and lithium-disilicate glass–ceramic (CAD = IPS e.max CAD, IvoclarVivadent). CAD/CAM veneers were either cemented with resin cements (P = Panavia21, KurarayDental), (R = RelyX Ultimate, 3M ESPE), (M = Multilink Automix, Ivoclar Vivadent) or fused with fusion glass–ceramic (C = CrystalConnect, IvoclarVivadent). A three-point bending test (15 mm span, zirconia on tension side) was performed on Instron universal testing machine (ISO 6872) recording load-to-failure (LTF) of first veneer cracks or catastrophic failure. For group VM, PM, TF-M, TF-C, CAD-M, CAD-C, ten more bars were prepared and aged with cyclic loading (100,000 cycles, 50% LTF) and thermocycling (2000 cycles) before testing. Data were analyzed by ANOVA, Tukey HSD post hoc tests, and t-test (α = 0.05). Zirconia veneered with IPS e.max CAD by fusing had significantly higher failure load compared with zirconia veneered with other veneering materials (p ≤ 0.05). For cemented veneers, the cement type had a significant effect on the failure load of the veneer zirconia specimens. Specimens cemented with Panavia 21 had a lower resistance to loading than other cements. The aging experiment revealed a significant difference in failure load between non-aged and aged bars in groups VM and PM, but not in the groups with CAD-/CAM-milled veneers. In conclusion, veneer materials, veneering methods, and cement materials have a significant effect on the failure load of bilayered veneer zirconia. CAD-/CAM-milled veneer zirconia is not susceptible to aging performed in this study

Effect of Ceramic Surface Treatment and Adhesive Systems on Bond Strength of Metallic Brackets

Objective. This study evaluated the effect of ceramic surface treatments on bond strength of metal brackets to machinable ceramics and veneering porcelain using different adhesive resins. Materials and methods. Machined ceramic specimens (10 × 10 × 2 mm) were prepared from Vitablocs mark II (Vita) and IPS e.max® CAD (Ivoclar). Layered porcelain fused to metal (IPS d.Sign®, Ivoclar) was used to fabricate PFM specimens (n = 60/group). Half of specimens were etched (9.6% HF, 15 sec), and the rest were nonetched. Three resin bonding systems were used for attaching metal brackets (Victory series™ APC II, 3M) to each group (n = 10): Transbond™ XT (3M), Light Bond™ (Reliance), or Blugloo™ (Ormco), all cured with LED curing unit (Bluephase G1600, Vivadent) for 50 s each. Specimens were immersed in deionized water at 37°C for 24 hours prior to shear bond testing (Instron) at crosshead speed of 0.5 mm/min. Debond surface of ceramic and bracket base was examined for failure mode (FM), Ceramic Damage Index (CDI), and Adhesive Remnant Index (ARI). ANOVA and post hoc multiple comparisons were used to analyze the differences in bond strength. The chi-squared test was used to determine significance effect of FM, CDI, and ARI. Results. Significant differences in shear bond strength among group were found (p≤0.05) related to ceramic, surface treatment, and resin cement. Conclusion. Bond strength of bracket to ceramic is affected by type of ceramic, resin cement, and ceramic surface conditioning. Etching ceramic surface enhanced ceramic-bracket bond strength. However, bond strengths in nontreated ceramic surface groups were still higher than bond strength required for bonding in orthodontic treatment