Analysis of solutions dedicated to non-conformity prevention

Research financed from the NCN research project no. UMO-2012/05 / B / HS4 / 04139.Purpose: The purpose of the work is to analyze the use of solutions aimed at preventing incompatibilities and assess the effectiveness of these solutions to minimize the risk associated with the quality of manufactured products. The analysis presented in the work is based on the Poka-Yoke error prevention concept used in the selected enterprise. The paper presents the state of the company before and after the introduction of Poka-Yoke method. Approach/Methodology/Design: The research methods used in the paper were the analysis of company data, analysis of literature states, as well as methods using the Poka-Yoke concept. The company was surveyed in 2017/2018 during the system implementation. Findings: The results on the control card for nonconforming units of type "p" were also presented, where the analysis showed that the introduction of the Poka-Yoke system to the company reduced the number of nonconformities by 6.69%. Practical Implications: Implementation of the Poka-Yoke system will allow enterprises to create conditions in which an error cannot happen, or will be immediately visible. The result of such an action may be distracting operators from repetitive operations, reducing the number of deficiencies and induce immediate action when a problem occurs. Originality/Value: Showing how the use of the Poka-Yoke system affects the prevention of inadvertent error in enterprises, by determining the differences between the state of the enterprise before and after the introduction of the Poka-Yoke system, there is an opportunity to improve the economy and contribute to the social and economic sphere.peer-reviewe

The impact of supply disruption on the standing order system in the FMCG supply chain

Purpose: The article aims to present the impact of supply disruption on the FMCG supply chain's standing order system. Design/Methodology/Approach: The research methodology used is the inventory control system's indicators with a fixed order quantity and graphic modeling. Findings: The system with a fixed order quantity requires the determination of the average annual demand for a given good and the determination of the ordering point, which is important for the FMCG branch. Practical Implications: Delivering goods in the right place and time plays an important role in the supply chain of movable goods. Maintaining a safe level of inventory ensures an effective and smooth flow of inventories through the supply chain's links. Originality/value: This article demonstrates the system with a fixed order quantity allows you to determine the size of the average annual demand for a given good and the point of ordering. It was indicated when logistic companies should use this method.peer-reviewe

Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria

Background: Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use. Results: To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation. Conclusion: The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species

Symplasmic isolation marks cell fate changes during somatic embryogenesis

Cell-to-cell signalling is a major mechanism controlling plant morphogenesis. Transport of signalling molecules through plasmodesmata is one way in which plants promote or restrict intercellular signalling over short distances. Plasmodesmata are membrane-lined pores between cells that regulate the intercellular flow of signalling molecules through changes in their size, creating symplasmic fields of connected cells. Here we examine the role of plasmodesmata and symplasmic communication in the establishment of plant cell totipotency, using somatic embryo induction from Arabidopsis explants as a model system. Cell-to-cell communication was evaluated using fluorescent tracers, supplemented with histological and ultrastructural analysis, and correlated with expression of a WOX2 embryo reporter. We showed that embryogenic cells are isolated symplasmically from non-embryogenic cells regardless of the explant type (immature zygotic embryos or seedlings) and inducer system (2,4-dichlorophenoxyacetic acid or the BABY BOOM (BBM) transcription factor), but that the symplasmic domains in different explants differ with respect to the maximum size of molecule capable of moving through the plasmodesmata. Callose deposition in plasmodesmata preceded WOX2 expression in future sites of somatic embryo development, but later was greatly reduced in WOX2-expressing domains. Callose deposition was also associated with a decrease DR5 auxin response in embryogenic tissue. Treatment of explants with the callose biosynthesis inhibitor 2-deoxy-D-glucose supressed somatic embryo formation in all three systems studied, and also blocked the observed decrease in DR5 expression. Together these data suggest that callose deposition at plasmodesmata is required for symplasmic isolation and establishment of cell totipotency in Arabidopsis

Looking for perspectives! EU energy policy in context

Transitioning to less carbon-intensive energy systems involves making difficult choices and priorities. This chapter imagines three individuals who are affected in different ways by EU energy policy. Their fictional stories illustrate that energy policies are embedded in social, historical and cultural practices and need to take a broader perspective than either technological fixes or a narrowly defined goal of low or zero carbon emissions to be fair and effective. We argue that this is often not reflected in the EU\u27s energy policy frameworks, and use the Energy Roadmap 2050 to demonstrate our point. Contrary to the impression given by the roadmap, a narrow technocratic empirical basis for a policy is not enough to define and solve an energy problem. Energy issues are societal problems and need to be addressed as such

The SERRATE protein is involved in alternative splicing in <em>Arabidopsis thaliana</em>

Howalternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcript-ase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 50 splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not cor-respond to the changes observed in the se-1mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1andDCL1, and is similar to the regu-lation of AS in which CBC is involved

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slflocus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment

siRNAs: Potential therapeutic agents against Hepatitis C Virus

Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects such as anemia, depression and "flu-like" symptoms. Needless to mention, the effectiveness of interferon therapy is predominantly, if not exclusively, limited to virus type 3a and 3b whereas in Europe and North America the majority of viral type is 1a and 2a. Due to the limited efficiency of current therapy, RNA interference (RNAi) a novel regulatory and powerful silencing approach for molecular therapeutics through a sequence-specific RNA degradation process represents an alternative option. Several reports have indicated the efficiency and specificity of synthetic and vector based siRNAs inhibiting HCV replication. In the present review, we focused that combination of siRNAs against virus and host genes will be a better option to treat HC