Nuclear organization of cholinergic, putative catecholaminergic, serotonergic and orexinergic systems in the brain of the African pygmy mouse (Mus minutoides) : organizational complexity is preserved in small brains

This study investigated the nuclear organization of four immunohistochemically identifiable neural systems (cholinergic, catecholaminergic, serotonergic and orexinergic) within the brain of the African pygmy mouse (Mus minutoides). The African pygmy mice studied had a brain mass of around 275 mg, making these the smallest rodent brains to date in which these neural systems have been investigated. In contrast to the assumption that in this small brain there would be fewer subdivisions of these neural systems, we found that all nuclei generally observed for these systems in other rodent brains were also present in the brain of the African pygmy mouse. As with other rodents previously studied in the subfamily Murinae, we observed the presence of cortical cholinergic neurons and a compactly organized locus coeruleus. These two features of these systems have not been observed in the non-Murinae rodents studied to date. Thus, the African pygmy mouse displays what might be considered a typical Murinae brain organization, and despite its small size, the brain does not appear to be any less complexly organized than other rodent brains, even those that are over 100 times larger such as the Cape porcupine brain. The results are consistent with the notion that changes in brain size do not affect the evolution of nuclear organization of complex neural systems. Thus, species belonging to the same order generally have the same number and complement of the subdivisions, or nuclei, of specific neural systems despite differences in brain size, phenotype or time since evolutionary divergence.The South African National Research Foundation (PRM, NCB), SIDA (KF) and by a fellowship within the Postdoc-Programme of the German Academic Exchange Service, DAAD (NP).http://www.elsevier.com /locate/jchemneuab201

The role of knowledge in a democratic society: investigations into mediation and change-oriented learning in water management practices

This project emerged from two previous Water Research Commission (WRC) research projects. In 2006 Heila Lotz-Sisitka and Jane Burt (Lotz-Sisitka, 2006) undertook research on participation in the establishment of integrated water resources management (IWRM) structures. They found that while much emphasis had gone into the establishment of water re-sources management structures, very little attention was being given to building people’s capacity to participate effectively in these structures. Access to and the ability to make use of knowledge resources about wa-ter resources management is a key aspect of such capacity building

Highly Divergent Mitochondrial ATP Synthase Complexes in Tetrahymena thermophila

Tetrahymena ATP synthase, an evolutionarily divergent protein complex, has a very unusual structure and protein composition including a unique Fo subunit a and at least 13 proteins with no orthologs outside of the ciliate lineage

May Measurement Month 2018: a pragmatic global screening campaign to raise awareness of blood pressure by the International Society of Hypertension

Aims Raised blood pressure (BP) is the biggest contributor to mortality and disease burden worldwide and fewer than half of those with hypertension are aware of it. May Measurement Month (MMM) is a global campaign set up in 2017, to raise awareness of high BP and as a pragmatic solution to a lack of formal screening worldwide. The 2018 campaign was expanded, aiming to include more participants and countries. Methods and results Eighty-nine countries participated in MMM 2018. Volunteers (≥18 years) were recruited through opportunistic sampling at a variety of screening sites. Each participant had three BP measurements and completed a questionnaire on demographic, lifestyle, and environmental factors. Hypertension was defined as a systolic BP ≥140 mmHg or diastolic BP ≥90 mmHg, or taking antihypertensive medication. In total, 74.9% of screenees provided three BP readings. Multiple imputation using chained equations was used to impute missing readings. 1 504 963 individuals (mean age 45.3 years; 52.4% female) were screened. After multiple imputation, 502 079 (33.4%) individuals had hypertension, of whom 59.5% were aware of their diagnosis and 55.3% were taking antihypertensive medication. Of those on medication, 60.0% were controlled and of all hypertensives, 33.2% were controlled. We detected 224 285 individuals with untreated hypertension and 111 214 individuals with inadequately treated (systolic BP ≥ 140 mmHg or diastolic BP ≥ 90 mmHg) hypertension. Conclusion May Measurement Month expanded significantly compared with 2017, including more participants in more countries. The campaign identified over 335 000 adults with untreated or inadequately treated hypertension. In the absence of systematic screening programmes, MMM was effective at raising awareness at least among these individuals at risk

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

A socio-environmental history of water in the Karoo c.1762-2012, with specific focus on Prince Albert and Williston

Thesis (MA)--Stellenbosch University, 2013.ENGLISH ABSTRACT: This study examines the history of water in two small Karoo towns. The main argument of the thesis is that the availability of and access to water has played influential roles in the development of Williston and Prince Albert. The ambitions of this thesis are three-fold: firstly, the approach is socio-environmental history and therefore it provides insight into the environmental as well as the social history of the resource in each region. It consciously reflects on the notion of power and explores the manner in which access to water was racialised by authorities in each town. Secondly, this thesis provides insight into the technological and legislative water supply and development of sanitation at both settlements, which is outlined in a broader national development to contextualise the local trajectories. Thirdly, through personal narratives it offers an ethnographic analysis of mind-sets such as fatalism and hopefulness in the face of extreme climatic conditions are examined. The experiences of the socially marginalised and underrepresented are intended to challenge the whiggish celebration of anthropogenic ascendancy over nature in the historiography.AFRIKAANSE OPSOMMING: Hierdie studie ondersoek die geskiedenis van water in twee klein Karoo dorpies. The hoof argument van die tesis is dat die beskikbaarheid van en toegang tot water bepalende rolle gespeel het in die ontwikkeling van Williston en Prins Albeit. Die doelstellings van hierdie tesis is drievoudig: eerstens, is die benadering sosio-omgewingsgeskiedenis en verskaf dit dus insig oor die omgewings- sowel as die sosiale geskiedenis van hierdie natuurlike hulpbron in elke streek. Hierdie werk weerspieel doelbewus die idee van mag en ondersoek die wyse waarop toegang tot water deur die dorpsowerhede rasbevooroordeeld was. Tweedens, verskaf hierdie tesis insig oor die water toevoer en die ontwikkeling van sanitere geriewe in beide nedersettings, waama die plaaslike ontwikkeling gesien word binne ' n breer nasionale konteks. Derdens word daar deur middel van persoonlike vertellings gekyk na die opkoms van etnografiese ingesteldhede soos fatalisme en hoop wanneer uiterste klimaatstoestande in die gesig gestaar word. Die ervannge van die sosiaal gemarginaliseerde en die onderverteenwoordigde groepe word uitgelig ten einde die liberate viering van antropogeniese dorninasie oor die natuur uit te daag