Investigation of possible virulence factors in Candida strains isolated from blood cultures

Background: The Candida species, which are one of the most common causes of nosocomial bloodstream infections, present with high mortality and morbidity rates. This study aims to investigate the production of esterase, phospholipase, proteinase, and biofilm formation ability of the Candida strains isolated from the blood cultures.Materials and Methods: Between June 2011 and July 2012, the Candida strains, which were isolated from blood cultures of a total of 50 patients, were studied. The esterase activity was analyzed in the Tween.80 agar, while phospholipase activity was studied in the egg yolk agar. The proteinase activity and biofilm formation were identified by using the petridish method and microplate method, respectively.Results: Of 50 specimens obtained from individual patients, 17 (34%) were identified as C. albicans, 14 (28%) as C. glabrata, 9 (18%) as C. parapsilosis, 5 (10%) as C. krusei, 4 (8%) as C. kefyr, and 1 (2%) as C. tropicalis. The rate of proteinase, phospholipase, and esterase positivity was higher in the C. albicans isolates. Biofilm formation was the highest in the C. parapsilosis strains.Conclusions: Higher rate of virulence factors in the most commonly isolated Candida species than other species indicates that these virulence factors play a crucial role in the pathogenesis.Key words: Biofilm, blood culture, candidemia, esterase, phospholipase, proteinas

Tasks for Agent-Based Negotiation Teams:Analysis, Review, and Challenges

An agent-based negotiation team is a group of interdependent agents that join together as a single negotiation party due to their shared interests in the negotiation at hand. The reasons to employ an agent-based negotiation team may vary: (i) more computation and parallelization capabilities, (ii) unite agents with different expertise and skills whose joint work makes it possible to tackle complex negotiation domains, (iii) the necessity to represent different stakeholders or different preferences in the same party (e.g., organizations, countries, and married couple). The topic of agent-based negotiation teams has been recently introduced in multi-agent research. Therefore, it is necessary to identify good practices, challenges, and related research that may help in advancing the state-of-the-art in agent-based negotiation teams. For that reason, in this article we review the tasks to be carried out by agent-based negotiation teams. Each task is analyzed and related with current advances in different research areas. The analysis aims to identify special challenges that may arise due to the particularities of agent-based negotiation teams.Comment: Engineering Applications of Artificial Intelligence, 201

Ensemble-based multi-filter feature selection method for DDoS detection in cloud computing

Widespread adoption of cloud computing has increased the attractiveness of such services to cybercriminals. Distributed denial of service (DDoS) attacks targeting the cloud’s bandwidth, services and resources to render the cloud unavailable to both cloud providers, and users are a common form of attacks. In recent times, feature selection has been identified as a pre-processing phase in cloud DDoS attack defence which can potentially increase classification accuracy and reduce computational complexity by identifying important features from the original dataset during supervised learning. In this work, we propose an ensemble-based multi-filter feature selection method that combines the output of four filter methods to achieve an optimum selection. We then perform an extensive experimental evaluation of our proposed method using intrusion detection benchmark dataset, NSL-KDD and decision tree classifier. The findings show that our proposed method can effectively reduce the number of features from 41 to 13 and has a high detection rate and classification accuracy when compared to other classification techniques

ICAR: endoscopic skull‐base surgery

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Case Report. Acremonium falciforme fungemia in a patient with acute leukaemia

We describe a case of Acremonium falciforme fungemia under treatment of fluconazole. A . falciforme is a common saprophyte. This fungus has been isolated from a patient's specimen, and the organism may have contributed to his death

Case report. Fatal Aspergillus flavus pericarditis in a patient with acute myeloblastic leukaemia

We report a case of Aspergillus flavus pericarditis treated with fluconazole for oral candidosis. The patient with acute myeloblastic leukaemia developed tachypnoea after antileukaemic chemotherapy. Pericardial effusion was seen in the echocardiogram. Aspergillus flavus was isolated from the pericardial fluid. The patient died from aspergillosis, before the antimycotic treatment could be changed to amphotericin B

Case reports. Trichosporon mucoides infection in three premature newborns

In the present study Trichosporon mucoides infections in 3 premature newborns are reported