To Explore the Research and Development Competence and School-to-Work Transition for Hospitality Students

This research focuses on the research and development competence and school-to-work transition on occupation selection for hospitality students with the use of social cognitive career theory. The positive attitude construct is the most identifiable for the research and development competences. For the school-to-work constructs, the most identifiable is the construct of learning and exploration, while the most identifiable among the self-efficacy constructs is the personal work attitude construct. The students' research and development competence and school-to-work transition to occupation selection are revealed. While the expectation result of professional competence is not significant; the others show significantly positive impact

Ser-634 and Ser-636 of Kaposi’s Sarcoma-Associated Herpesvirus RTA are Involved in Transactivation and are Potential Cdk9 Phosphorylation Sites

The replication and transcription activator (RTA) of Kaposi’s sarcoma-associated herpesvirus (KSHV), K-RTA, is a lytic switch protein that moderates the reactivation process of KSHV latency. By mass spectrometric analysis of affinity purified K-RTA, we showed that Thr-513 or Thr-514 was the primary in vivo phosphorylation site. Thr-513 and Thr-514 are proximal to the nuclear localization signal (527KKRK530) and were previously hypothesized to be target sites of Ser/Thr kinase hKFC. However, substitutions of Thr with Ala at 513 and 514 had no effect on K-RTA subcellular localization or transactivation activity. By contrast, replacement of Ser with Ala at Ser-634 and Ser-636 located in a Ser/Pro-rich region of K-RTA, designated as S634A/S636A, produced a polypeptide with ∼10 kDa shorter in molecular weight and reduced transactivation in a luciferase reporter assay relative to the wild type. In contrast to prediction, the decrease in molecular weight was not due to lack of phosphorylation because the overall Ser and Thr phosphorylation state in K-RTA and S634A/S636A were similar, excluding that Ser-634 or Ser-636 motif served as docking sites for consecutive phosphorylation. Interestingly, S634A/S636A lost ∼30% immuno-reactivity to MPM2, an antibody specific to pSer/pThr-Pro motif, indicating that 634SPSP637 motif was in vivo phosphorylated. By in vitro kinase assay, we showed that K-RTA is a substrate of CDK9, a Pro-directed Ser/Thr kinase central to transcriptional regulation. Importantly, the capability of K-RTA in associating with endogenous CDK9 was reduced in S634A/S636A, which suggested that Ser-634 and Ser-636 may be involved in CDK9 recruitment. In agreement, S634A/S636A mutant exhibited ∼25% reduction in KSHV lytic cycle reactivation relative to that by the wild type K-RTA. Taken together, our data propose that Ser-634 and Ser-636 of K-RTA are phosphorylated by host transcriptional kinase CDK9 and such a process contributes to a full transcriptional potency of K-RTA

Genotoxic Klebsiella pneumoniae in Taiwan

Colibactin is a nonribosomal peptide-polyketide synthesized by multi-enzyme complexes encoded by the pks gene cluster. Colibactin-producing Escherichia coli have been demonstrated to induce host DNA damage and promote colorectal cancer (CRC) development. In Taiwan, the occurrence of pyogenic liver abscess (PLA) has been suggested to correlate with an increasing risk of CRC, and Klebsiella pneumoniae is the predominant PLA pathogen in Taiwan

Application of Ground LiDAR for The Investigation of Outcrop Discontinuities and Estimation of Associated Engineering Characteristics

Reliable and direct information about rock slope materials and discontinuity parameters from field investigation are keys to engineering design, structure maintenance and soil/water conservation. Under the impact of global climate change and the surge in extreme weather events, rapid and accurate equipment or procedures for geological engineering surveys are necessary to harmonize engineering facilities with natural environment. This study utilizes 3D laser scanning technology (also known as ground LiDAR), which obtains the coordinates of surface geometry rapidly in point clouds, to survey two outcrops. Applying ground LiDAR in a geological survey, we compare the results with those provided by the International Society of Rock Mechanics, and analyze the slope stability. This study also discusses the applicability and limitations of ground LiDAR technology in discontinuity investigation and estimation of rock mass engineering characteristics. Suggested operating procedures for the use of ground LiDAR technology in outcrop surveys are provided accordingly. The results show that ground LiDAR technology reduces the in-situ operation time significantly, thus facilitating efficient discontinuity surveys and outcrop parameters evaluation. However, the quantities of office work and the accuracy of point clouds depend on the velocity and accuracy of ground LiDAR surveys. Therefore, inspection of survey results and output parameter, along with the application and operating specifications of geological surveys, including point cloud resolution, reasonable error of superimposition, inspection procedures and data analysis, still await further research.岩石工程道路邊坡經現地調查獲得可靠、直接的岩石材料種類與不連續面參數，為確保工程設計施工、設施營運維護及環境水土保持之關鍵資訊。在近年全球氣候變遷與極端氣候事件規模與頻率加大的衝擊下，迅速、精準的工程地質調查工具與作業方法，為落實工程設施與自然環境永續發展的不二法門。本研究利用三維雷射掃描技術 (又稱光達技術，|iDAR)，運用其可快速取得露頭表面幾何坐標描述點雲的特性，透過2處露頭現地地質調查，探討地面光達在工址地質調查之應用，並比較國際岩石力學學會建議工址現地調查作業方法所獲得的參數，分析評估邊坡的穩定特性，據以提出地面光達應用於露頭不連續面調查與岩體工程特性評估的適用性與限制，並提出地面光達應用於露頭調查的作業程序。研究結果顯示，地面光達技術可大幅縮減現地調查作業時間，為露頭不連續面調查與參數評估的新利器，由於室內分析工作量和點雲的精度決定了|iDAR應用於露頭不連續面調查的速度與精準度，未來針對調查成果與產出參數的檢核、在地質調查的應用與作業規範，包括點雲解析度、疊合容許誤差以及資料分析與檢核程序等，有待進一步深入研究

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe

Combined searches for the production of supersymmetric top quark partners in proton-proton collisions at root s=13 TeV

A combination of searches for top squark pair production using proton-proton collision data at a center-of-mass energy of 13 TeV at the CERN LHC, corresponding to an integrated luminosity of 137 fb(-1) collected by the CMS experiment, is presented. Signatures with at least 2 jets and large missing transverse momentum are categorized into events with 0, 1, or 2 leptons. New results for regions of parameter space where the kinematical properties of top squark pair production and top quark pair production are very similar are presented. Depending on themodel, the combined result excludes a top squarkmass up to 1325 GeV for amassless neutralino, and a neutralinomass up to 700 GeV for a top squarkmass of 1150 GeV. Top squarks with masses from 145 to 295 GeV, for neutralino masses from 0 to 100 GeV, with a mass difference between the top squark and the neutralino in a window of 30 GeV around the mass of the top quark, are excluded for the first time with CMS data. The results of theses searches are also interpreted in an alternative signal model of dark matter production via a spin-0 mediator in association with a top quark pair. Upper limits are set on the cross section for mediator particle masses of up to 420 GeV

Measurement of B-c(2S)(+) and B-c*(2S)(+) cross section ratios in proton-proton collisions at root s=13 TeV

Peer reviewe

Probing effective field theory operators in the associated production of top quarks with a Z boson in multilepton final states at root s=13 TeV

Peer reviewe