Submission to the Province of Nova Scotia on Its Review of the Intimate Images and Cyber-Protection Act - LEAF

The Women’s Legal Education and Action Fund (LEAF) commends the Nova Scotia government for reviewing its Intimate Images and Cyber-protection Act (the Act) and seeking public input for this review. Nova Scotia has been, and continues to be, a leader in Canada for its role in advancing innovative laws and supports for people targeted by technology-facilitated violence (TFV), digital abuse, and the non-consensual distribution of intimate images (NCDII). As these forms of harmful behaviour evolve and become better understood, it is important to revisit this legislation to assess whether it is providing meaningful and accessible responses to such serious social harms. This consideration is especially important for equality-deserving groups who often experience the brunt of abusive behaviour online.LEAF recognizes that these forms of harms disproportionately impact historically marginalized communities. Women, girls, and gender-diverse people, particularly those with intersecting marginalized identities, including Indigenous, Black, people of colour, members of 2SLGBTQQIA communities, and people with differing abilities, face higher rates of targeted attacks that focus on their gender, race, sexual orientation, gender identity, gender expression, and ability. These attacks can have devastating consequences on the health and wellbeing of those targeted.This submission provides recommendations on changes to Nova Scotia\u27s Intimate Images and Cyber-protection Act and its regulations, as well as recommendations on aspects of CyberScan. This submission relies on and builds on previous recommendations made in LEAF’s Deplatforming Misogyny report, the Uniform Non-Consensual Disclosure of Intimate Images Act, and Alexa Dodge’s Deleting Digital Harm: A Review of Nova Scotia’s CyberScan Unit report. Our recommendations focus on providing more expedient and direct supports to those experiencing TFV and NCDII; expanding the scope of the definition of intimate images and reconsidering the parameters of “public interest”; and broadening the scope of research and educational materials to ensure that they are survivor-centric and to cover the systemic and social context that results in digital abuse and NCDII

Submission to the Toronto Police Services Board’s Use of New Artificial Intelligence Technologies Policy- LEAF and the Citizen Lab

We write as a group of experts in the legal regulation of artificial intelligence (AI), technology-facilitated violence, equality, and the use of AI systems by law enforcement in Canada. We have experience working within academia and legal practice, and are affiliated with LEAF and the Citizen Lab who support this letter.We reviewed the Toronto Police Services Board Use of New Artificial Intelligence Technologies Policy and provide comments and recommendations focused on the following key observations:1. Police use of AI technologies must not be seen as inevitable2. A commitment to protecting equality and human rights must be integrated more thoroughly throughout the TPSB policy and its AI analysis procedures3. Inequality is embedded in AI as a system in ways that cannot be mitigated through a policy only dealing with use4. Having more accurate AI systems does not mitigate inequality5. The TPS must not engage in unnecessary or disproportionate mass collection and analysis of data6. TPSB’s AI policy should provide concrete guidance on the proactive identification and classification of risk7. TPSB’s AI policy must ensure expertise in independent vetting, risk analysis, and human rights impact analysis8. The TPSB should be aware of assessment challenges that can arise when an AI system is developed by a private enterprise9. The TPSB must apply the draft policy to all existing AI technologies that are used by, or presently accessible to, the Toronto Police ServiceIn light of these key observations, we have made 33 specific recommendations for amendments to the draft policy

Identification and characterization of the orf virus type I topoisomerase

AbstractVaccinia virus (VV) and Shope fibroma virus (SFV), representatives of the orthopox and leporipox genera, respectively,encode type I DNA topoisomerases. Here we report that the 957-nt F4R open reading frame of orf virus (OV), a representative of the parapox genus, is predicted to encode a 318-aa protein with extensive homology to these enzymes. The deduced amino acid sequence of F4R has 54.7 and 50.6% identity with the VV and SFV enzymes, respectively. One hundred forty amino acids are predicted to be conserved in all three proteins. The F4R protein was expressed in Escherichia coli under the control of an inducible T7 promoter, partially purified, and shown to be a bona fide type I topoisomerase. Like the VV enzyme, the OV enzyme relaxed negatively supercoiled DNA in the absence of divalent cations or ATP and formed a transient covalent intermediate with cleaved DNA that could be visualized by SDS-PAGE. Both the noncovalent and covalent protein/DNA complexes could be detected in an electrophoretic mobility shift assay. The initial PCR used to prepare expression constructs yielded a mutant allele of the OV topoisomerase with a G-A transition at nt 677 that was predicted to replace a highly conserved Tyr residue with a Cys. This allele directed the expression of an enzyme which retained noncovalent DNA binding activity but was severely impaired in DNA cleavage and relaxation. Incubation of pUC19 DNA with the wild-type OV or VV enzyme yielded an indistinguishable set of DNA cleavage fragments, although the relative abundance of the fragments differed for the two enzymes. Using a duplex oligonucleotide substrate containing the consensus site for the VV enzyme, we demonstrated that the OV enzyme also cleaved efficiently immediately downstream of the sequence CCCTT↓

Mechanics rules cell biology

Cells in the musculoskeletal system are subjected to various mechanical forces in vivo. Years of research have shown that these mechanical forces, including tension and compression, greatly influence various cellular functions such as gene expression, cell proliferation and differentiation, and secretion of matrix proteins. Cells also use mechanotransduction mechanisms to convert mechanical signals into a cascade of cellular and molecular events. This mini-review provides an overview of cell mechanobiology to highlight the notion that mechanics, mainly in the form of mechanical forces, dictates cell behaviors in terms of both cellular mechanobiological responses and mechanotransduction

Gene Expression Changes in the Prefrontal Cortex, Anterior Cingulate Cortex and Nucleus Accumbens of Mood Disorders Subjects That Committed Suicide

Suicidal behaviors are frequent in mood disorders patients but only a subset of them ever complete suicide. Understanding predisposing factors for suicidal behaviors in high risk populations is of major importance for the prevention and treatment of suicidal behaviors. The objective of this project was to investigate gene expression changes associated with suicide in brains of mood disorder patients by microarrays (Affymetrix HG-U133 Plus2.0) in the dorsolateral prefrontal cortex (DLPFC: 6 Non-suicides, 15 suicides), the anterior cingulate cortex (ACC: 6NS, 9S) and the nucleus accumbens (NAcc: 8NS, 13S). ANCOVA was used to control for age, gender, pH and RNA degradation, with P≤0.01 and fold change±1.25 as criteria for significance. Pathway analysis revealed serotonergic signaling alterations in the DLPFC and glucocorticoid signaling alterations in the ACC and NAcc. The gene with the lowest p-value in the DLPFC was the 5-HT2A gene, previously associated both with suicide and mood disorders. In the ACC 6 metallothionein genes were down-regulated in suicide (MT1E, MT1F, MT1G, MT1H, MT1X, MT2A) and three were down-regulated in the NAcc (MT1F, MT1G, MT1H). Differential expression of selected genes was confirmed by qPCR, we confirmed the 5-HT2A alterations and the global down-regulation of members of the metallothionein subfamilies MT 1 and 2 in suicide completers. MTs 1 and 2 are neuro-protective following stress and glucocorticoid stimulations, suggesting that in suicide victims neuroprotective response to stress and cortisol may be diminished. Our results thus suggest that suicide-specific expression changes in mood disorders involve both glucocorticoids regulated metallothioneins and serotonergic signaling in different regions of the brain

Stem Cell Research Funding Policies and Dynamic Innovation: A Survey of Open Access and Commercialization Requirements

# The Author(s) 2014. This article is published with open access at Springerlink.com Abstract This article compares and contrasts the pressures of both open access data sharing and commercialization policies in the context of publicly funded embryonic stem cell research (SCR). First, normative guidelines of international SCR orga-nizations were examined. We then examined SCR funding guidelines and the project evaluation criteria of major funding organizations in the EU, the United Kingdom (UK), Spain, Canada and the United States. Our survey of policies revealed subtle pressures to commercialize research that include: in-creased funding availability for commercialization opportuni-ties, assistance for obtaining intellectual property rights (IPRs) and legislation mandating commercialization. In lieu of open access models, funders are increasingly opting for limited sharing models or “protected commons ” models that make the research available to researchers within the same region or those receiving the same funding. Meanwhile, there still is need for funding agencies to clarify and standardize terms such as “non-profit organizations ” and “for-profit research,” as more universities are pursuing for-profit or commercial opportunities. Keywords Stemcell research(SCR).Humanembryonicstem cells (hESC). Induced pluripotent stem cells (iPSC). Open access. Data sharing. Commercialization Abbreviations hESC human embryonic stem cells iPSC induced pluripotent stem cells IPRs intellectual property rights MTA material transfer agreement SCR stem cell research SLA simple letter agreement TTO technology transfer offic

Submission to the Province of Nova Scotia on Its Review of the Intimate Images and Cyber-Protection Act - LEAF

The Women’s Legal Education and Action Fund (LEAF) commends the Nova Scotia government for reviewing its Intimate Images and Cyber-protection Act (the Act) and seeking public input for this review. Nova Scotia has been, and continues to be, a leader in Canada for its role in advancing innovative laws and supports for people targeted by technology-facilitated violence (TFV), digital abuse, and the non-consensual distribution of intimate images (NCDII). As these forms of harmful behaviour evolve and become better understood, it is important to revisit this legislation to assess whether it is providing meaningful and accessible responses to such serious social harms. This consideration is especially important for equality-deserving groups who often experience the brunt of abusive behaviour online.LEAF recognizes that these forms of harms disproportionately impact historically marginalized communities. Women, girls, and gender-diverse people, particularly those with intersecting marginalized identities, including Indigenous, Black, people of colour, members of 2SLGBTQQIA communities, and people with differing abilities, face higher rates of targeted attacks that focus on their gender, race, sexual orientation, gender identity, gender expression, and ability. These attacks can have devastating consequences on the health and wellbeing of those targeted.This submission provides recommendations on changes to Nova Scotia\u27s Intimate Images and Cyber-protection Act and its regulations, as well as recommendations on aspects of CyberScan. This submission relies on and builds on previous recommendations made in LEAF’s Deplatforming Misogyny report, the Uniform Non-Consensual Disclosure of Intimate Images Act, and Alexa Dodge’s Deleting Digital Harm: A Review of Nova Scotia’s CyberScan Unit report. Our recommendations focus on providing more expedient and direct supports to those experiencing TFV and NCDII; expanding the scope of the definition of intimate images and reconsidering the parameters of “public interest”; and broadening the scope of research and educational materials to ensure that they are survivor-centric and to cover the systemic and social context that results in digital abuse and NCDII

Stem Cell Research Funding Policies and Dynamic Innovation: A Survey of Open Access and Commercialization Requirements

This article compares and contrasts the pressures of both open access data sharing and commercialization policies in the context of publicly funded embryonic stem cell research (SCR). First, normative guidelines of international SCR organizations were examined. We then examined SCR funding guidelines and the project evaluation criteria of major funding organizations in the EU, the United Kingdom (UK), Spain, Canada and the United States. Our survey of policies revealed subtle pressures to commercialize research that include: increased funding availability for commercialization opportunities, assistance for obtaining intellectual property rights (IPRs) and legislation mandating commercialization. In lieu of open access models, funders are increasingly opting for limited sharing models or “protected commons” models that make the research available to researchers within the same region or those receiving the same funding. Meanwhile, there still is need for funding agencies to clarify and standardize terms such as “non-profit organizations” and “for-profit research,” as more universities are pursuing for-profit or commercial opportunities