RDW-CV E RDW-SD EM GATOS DOMÉSTICOS SAUDÁVEIS

O objetivo do presente estudo foi estabelecer intervalo de referência para valores de RDW-CV e RDW-SD em felinos clinicamente sadios. Amostras de sangue de 187 felinos jovens e adultos, clinicamente sadios e encaminhados para procedimentos cirúrgico eletivos foram utilizadas. As amostras foram obtidas a partir da punção de veia jugular, armazenadas em tubos com EDTA e posteriormente processadas.  A determinação do RDW foi realizada utilizando o analisador hematológico veterinário modelo pocH-100iV DIFF (Sysmex®). O intervalo de normalidade estabelecido para o RDW-CV foi de 16,8±1,84% e do RDW-SD 31,8±2,83 fL. A análise estatística não mostrou diferença significativa entre sexo e faixa etária (teste Z, p>0,05).  Os valores médios para RDW-CV e RDW-SD apresentados podem ser utilizados como referência para felinos domésticos saudáveis, sem raça definida, independente do sexo ou idade

Frequência dos tipos sanguíneos de gatos domésticos mestiços no município de Campo Grande, Mato Grosso do Sul, Brasil

O objetivo deste estudo foi determinar as frequências dos tipos sanguíneos, do sistema AB, de gatos domésticos sem raça definida na cidade de Campo Grande, Mato Grosso do Sul, Brasil. Foram selecionados aleatoriamente 202 animais clinicamente saudáveis e a tipagem sanguínea foi realizada por meio do teste de hemaglutinação em tubo de ensaio. A distribuição da frequência foi de 98,5% e 1,5% para gatos dos tipos A e AB, respectivamente. Gatos do tipo B não foram identificados. Os resultados demostraram que no município de Campo Grande há predomínio de gatos domésticos mestiços do tipo sanguíneo A, contudo a presença de gatos com tipo AB e a ausência do tipo B, indicam peculiaridade da população escolhida. Estes dados alertam sobre a possibilidade de reações transfusionais adversas e a ocorrência de isoeritrólise neonatal, e contribui para a seleção adequada de doadores em banco de sangue animal

Práticas seguras na assistência cirúrgica: uma reflexão teórica

Objective: Carry out a theoretical reflection on safe practices related to surgical care.Objetivo: Realizar uma reflexão teórica sobre as práticas seguras relacionadas à assistência cirúrgica

Características citológicas de cistoadenocarcinoma papilar ovariano em um cão

Background: Ovarian papillary cystadenocarcinoma is a rare neoplasm associated with peritoneal implantation and malignant effusion. Most dogs are asymptomatic until the nodules become large and the abdominal volume is increased. From the clinical suspicion, the diagnosis can be obtained through imaging and histopathology, however, cytological analysis has become an alternative method for the early detection of this neoplasm. In order to demonstrate the importance of cytology in the diagnosis of ovarian neoplasms and its metastasis, it is reported a case of metastatic ovarian papillary cystadenocarcinoma in a dog.Case: Female, intact, teckel, 5-year-old, with increased abdominal volume. Physical examination revealed ascites and intracavitary mass, abdominocentesis and fine needle puncture of the mass were performed for cytological evaluation. In the cavity fluid it was observed: dark red color, cloudy appearance, hematocrit of 35%; (7.6 g / dL), pH (8.0), 22,000 nucleated cells / μL, marked cellularity of pleomorphic epithelial cells arranged in three-dimensional cohesive groups, sometimes in acinar or tubular pattern, nucleus with loose chromatin coarse, scarse to moderate cytoplasm, perinuclear halo, multiple and evident nucleoli compatible with carcinomatous neoplastic effusion. In the cytological evaluation of the tumor, epithelial cells were observed, with the same microscopic characteristics of the abdominal fluid. A laparotomy that did not show metastasis was performed, multiple nodes interspersed with cystic regions containing yellow-red fluid in the right ovary were visualized. Histopathology showed: neoplastic cells proliferation of ovarian glandular tissue, scarce cytoplasm, poorly delimited, nucleus ranging from oval to cylindrical with marked pleomorphism, evident nucleoli and loose chromatin, mitotic figures and papillary growth. Neoplastic cells forming irregular cavities with proteinaceous fluid, scarce connective tissue intermingling the cellular nest and areas of hemorrhage. Cytological and histopathological analyzes were compatible with ovarian papillary cystadenocarcinoma. After three months of excision, the dog returned with thoracic effusion that presented the same characteristics of the abdominal fluid, indicating metastasis.Discussion: Metastasis and effusion were observed in 48% and 86% of dogs with this tumor, respectively. Cell exfoliation, release of fluid through the tumor capsule or rupture of cysts can result in transcelomic metastatic implants that exert pressure and obstruct peritoneal and diaphragmatic lymphatic vessels causing effusion. The macro and microscopic characteristics of the abdominal effusion reinforced the suspicion of neoplasia, and the cytomorphological evaluation of the tumor, which identified carcinomatous cells similar to that of the effusion, allowed the presumptive diagnosis of the neoplasia. The macroscopic presence of multiple nodes interspersed with cystic regions containing red fluid in the right ovary, identified after surgical excision, reinforced the cytologic diagnosis. Histopathological examination identified wellestablished microscopic features that allowed the definitive and confirmatory diagnosis of neoplasia. Radiological analysis of the chest was not enough to detect the metastasis diagnosed by effusion analysis, however, small nodules (less than 6 mm) are difficult to identify by imaging. Thus, it is important to emphasize the importance of cytological evaluation of tumor and effusions for detection of neoplastic cells for the diagnosis of intracavitary neoplasia and metastasis

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Troponina, biomarcador de injúria cardíaca, na medicina veterinária: Revisão

As lesões no miocárdio são de difícil diagnóstico e podem ser confundidas com outras enfermidades, portanto é importante a associação de diferentes métodos diagnósticos para a conclusão. Na medicina humana as troponinas são reconhecidas, há vários anos, como biomarcadores sensíveis e específicos na identificação de lesões miocárdica. As troponinas cardíacas são expressas exclusivamente pelo miocárdio e liberadas para circulação logo após uma injúria no músculo cardíaco. Essa revisão teve como objetivo abordar os diferentes tipos de troponinas, dando enfoque a sua aplicabilidade e comportamento como marcador de lesão tecidual em animais. Constatou-se se que o uso da troponina I cardíaca (cTnI), como biomarcador de injuria cardíaca, foi validada para o uso diagnóstico em diferentes espécies animais e é capaz de colaborar no diagnóstico e prognóstico das enfermidades miocárdicas, embora, não determinem o mecanismo da lesão

Comportamento dos marcadores bioquímicos de injúria hepática nos cães com leishmaniose visceral

O objetivo deste estudo foi identificar as alterações dos marcadores bioquímicos de danos hepáticos em cães naturalmente infectados por Leishmania sp. de acordo com as diferentes formas clínicas apresentadas por esses animais. Sangue de 41 cães sororreagentes nos testes de ELISA e positivos nos testes imunocromatográfico e parasitológico de linfonodo, foram colhidos por venopunção e acondicionadas em tubo sem anticoagulante para obtenção de soro e mensuração de colesterol total, triglicerídeos, ureia, fosfatase alcalina, gamma glutamiltransferase, alanina aminotransferase, proteínas totais e albumina. Realizou-se a estratificação em grupos de acordo com a sintomatologia clínica, em assintomáticos (7/41), oligossintomáticos (15/41) e sintomáticos (19/41). Hiperglobulinemia, hiperproteinemia e diminuição da razão Albumina:Globulina foram constatadas em todos os grupos com diferença estatística significativa para albumina (2,87 ±0,45 g/dL). Colesterol, fosfatase alcalina, alanina aminotransferase e gamma glutamiltransferase permaneceram dentro dos intervalos de normalidade. As alterações encontradas nos marcadores bioquímicos de danos hepáticos sugerem que a leishmaniose visceral deve ser incluída no diagnóstico diferencial das hepatopatias, principalmente, nas áreas endêmicas para a enfermidade