The Defense Life Cycle Management System As A Working Model For Academic Application

Performing the review and assessment of masters’ level degree programs can be an overwhelming and challenging endeavor.  Getting organized and mapping out the entire review and assessment process can be extremely helpful and more importantly provide a path for successfully accomplishing the review and assessment of the entire program.  This paper will provide a roadmap that was used as a guide for accomplishing a successful review and assessment of academic degree programs in a logical and succinct manner by adapting a proven model originally developed for the acquisition and life cycle management of new equipment, materials, and systems. This paper will also discuss how the Integrated Defense Acquisition, Technology, and Logistics Life Cycle Management System process was adapted to the needs of an academic institution of higher-learning

α1D-Adrenoceptors are responsible for the high sensitivity and the slow time-course of noradrenaline-mediated contraction in conductance arteries

The objective of this study was to determine whether the different time-course characteristics of α1-adrenoceptor-mediated contraction in arteries can be related to the subtypes involved. Contractile responses to noradrenaline (NA) were compared with inositol phosphate accumulation and extracellular signal-regulated kinase (ERK)1/2 phosphorylation after α1-agonist stimuli in the same vessels in the presence or absence of α1-antagonists in rat or in α1-subtype knockout (KO) mice. Aorta, where α1D-AR is the main functional subtype, had higher sensitivity to NA (in respect of inositol phosphate [IP], pERK1/2, and contractile response) than tail artery, where the α1A-adrenoceptor subtype is predominant. Furthermore, the contraction in aorta exhibited a slower decay after agonist removal and this was consistent in all strains harboring α1D-adrenoceptors (from rat, α1B-KO, and wild-type [WT] mice) but was not observed in the absence of the α1D-adrenoceptor signal (α1D-adrenoceptor blocked rat aorta or aorta from α1D-KO). IP formation paralleled α1-adrenoceptor-mediated contraction (agonist present or postagonist) in aorta and tail artery. High sensitivity to agonist and persistence of response after agonist removal is a property of α1D-adrenoceptors. Therefore, the preponderance of this subtype in noninnervated conductance arteries such as aorta allows responsiveness to circulating catecholamines and prevents abrupt changes in vessel caliber when the stimulus fluctuates. Conversely, in innervated distributing arteries, high local concentrations of NA are required to activate α1A-adrenoceptors for a response that is rapid but short lived allowing fine adjustment of the contractile tone by perivascular sympathetic nerves

Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting

Aims: To compare the constitutive and agonist-dependent endosomal trafficking of α1A- and α1B-adrenoceptors (ARs) and to establish if the internalization pattern determines the signaling pathways of each subtype. Methods: Using CypHer5 technology and VSV-G epitope tagged α1A- and α1B-ARs stably and transiently expressed in HEK 293 cells, we analyzed by confocal microscopy the constitutive and agonist-induced internalization of each subtype, and the temporal relationship between agonist induced internalization and the increase in intracellular calcium (determined by FLUO-3 flouorescence), or the phosphorylation of ERK1/2 and p38 MAP kinases (determined by Western blot). Results and Conclusions: Constitutive as well as agonist-induced trafficking of α1A and α1B ARs maintain two different endosomal pools of receptors: one located close to the plasma membrane and the other deeper into the cytosol. Each subtype exhibited specific characteristics of internalization and distribution between these pools that determines their signaling pathways: α1A-ARs, when located in the plasma membrane, signal through calcium and ERK1/2 pathways but, when translocated to deeper endosomes, through a mechanism sensitive to β-arrestin and concanavalin A, continue signaling through ERK1/2 and also activate the p38 pathway. α1B-ARs signal through calcium and ERK1/2 only when located in the membrane and the signals disappear after endocytosis and by disruption of the membrane lipid rafts by methyl-β-cyclodextrin

Potential Relevance of α1-Adrenergic Receptor Autoantibodies in Refractory Hypertension

-AAB might have a mechanistic role and could represent a therapeutic target. in cardiomyocytes and induce mesentery artery segment contraction.-AAB in hypertensive patients, and the notion of immunity as a possible cause of hypertension

Passeio de Barco no Parque Farroupilha

-Porto Alegre -Bairro Bom Fim -Parque Farroupilha -Passeio de Barco -Casais -Mulher -CriançaCasais passeando de barco no lago. Nas margens do lago homens, mulheres e crianças observam o passeio. Foto tirada em 1947.Bo

„eSports“ – Nur ein Trend oder schon ein Wirtschaftszweig? : Chancen und Potentiale eines Wachstumsmarktes der Medien- und Unterhaltungsbranche in Deutschland

Die vorliegende Bachelorarbeit beschäftigt sich mit dem Phänomen der asiatischen und amerikanischen Trendsportart eSport. In Anbetracht der Tatsache, dass der eSport in anderen Ländern zugleich Wirtschaftsfaktor, als auch eine bereits anerkannte Sportart ist, soll unter-sucht werden, inwiefern sich hieraus potentielle Chancen in einem Wachs-tumsmarkt, für die deutsche Industrie aber auch für Werbetreibende und Sponsoren ergeben. Die Arbeit befasst sich demnach damit, wie der eSport in Deutschland in den Medien dargestellt wird, ob er weiterhin nur eine Trendsportart für eine Randgruppe bleibt oder sich nach und nach in naher Zukunft zu ei-nem profitablen Wirtschaftszweig entwickelt, der Sponsoren und Werbern neue Türen öffnet und so den klassischen Sportarten Sponsoring- und Werbeetats streitig machen wird

Storytelling- en kosmetisk gest eller reell delaktighet? : – En studie i användningen av strategiskt berättande hos två organisationersom arbetar för omställning.

I den här är fallstudien har vi valt att undersöka hur två organisationer som arbetar med klimatomställning använder storytelling. Den ena organisationen, EU, har drivit projektet Leader sedan 1990-talet, den andra är Svenska Naturskyddsföreningen som 2013 skapade en julkalender för sin hemsida. I studien undersöks sambandet mellan organisationernas bild av engagemang och berättelsernas struktur. Studien utgår från två teoretiska perspektiv Governmentality och narrativ teori. Sökningen av berättelserna har skett genom ett målstyrt urval och två kvalitativa metoder har tillämpats: text- och narrationsanalys

Neuropeptide Y stimulation of extracellular signal-regulated kinases in human erythroleukemia cells. J Pharmacol Exp Ther 291:1172–1178

ABSTRACT We have used human erythroleukemia (HEL) cells to investigate distal signaling mechanisms of neuropeptide-Y (NPY) receptors. NPY did not activate phospholipase D, determined as a phosphatidylethanol formation, or protein kinase C (PKC) determined enzymatically as a translocation to the plasma membrane. However, NPY caused a rapid (already maximal after 30 s) and concentration-dependent (maximum at 10 -100 nM) activation of extracellular signal-regulated kinase (ERK) as assessed by immunoblotting with epitope-specific, antiphosphotyrosine antibodies and in some cases enzymatically. ERK activation by 100 nM NPY was abolished by the Y 1 NPY receptor antagonist BIBP 3226 (1 M), pertussis toxin treatment (100 ng ml Ϫ1 overnight), the mitogen-activated protein kinase (MAPK) kinase inhibitor PD 98059 (100 M), and the phosphatidylinositol-3-kinase inhibitor wortmannin (100 nM). Whereas the PKC inhibitor staurosporine (3 M) inhibited ERK activation by NPY, the chemically distinct PKC inhibitors calphostin C (3 M), Gö 6976 (3 M), and bisindolylmaleimide I (3 M) did not. NPY did not activate other MAPK such as jun N-terminal kinase or p38 MAPK. We conclude that NPY does not activate phospholipase D, PKC, jun N-terminal kinase, or p38 MAPK in HEL cells. However, NPY activates ERK by a pathway involving Y 1 receptors, pertussis toxin-sensitive G proteins, and phosphatidylinositol-3-kinase, whereas PKC may not be involved. Staurosporine may have PKC-independent effects on ERK activation

alpha(1)-adrenoceptor subtypes differentially couple to growth promotion and inhibition in Chinese hamster ovary cells

We have compared the coupling of human alpha(1A)-, alpha(1B)-, and alpha(1D)-adrenoceptors (expressed at approximately 2000 fmol/mg protein in Chinese hamster ovary cells) to cellular growth promotion (as assessed by [(3)H]thymidine incorporation) and related signaling mechanisms. Maximum elevation of intracellular Ca(2+) by the three subtypes occurred with the rank order alpha(1A) (1691 nM) > alpha(1D) (1215 nM) > alpha(1B) (360 nM). In contrast, activation of the ERK, JNK, and p38 forms of mitogen-activated protein kinases occurred with the rank order alpha(1D) > alpha(1A) > alpha(1B). alpha(1A)-Adrenoceptor stimulation inhibited basal and growth factor-stimulated [(3)H]thymidine incorporation by 74%, and this was mitigated by p38 inhibition. In contrast, alpha(1D)-adrenoceptor stimulation enhanced cellular growth by 136%, and this was blocked by two distinct inhibitors of ERK activation. We conclude that within a given cell type alpha(1)-adrenoceptor subtypes can have opposite effects on cellular growth, although their proximal signal transduction displays only quantitative difference