A novel bioaugmentation technique effectively increases the skin-associated microbial diversity of captive eastern hellbenders

Captive environments are maintained in hygienic ways that lack free-flowing microbes found in animals’ natural environments. As a result, captive animals often have depauperate host-associated microbial communities compared to conspecifics in the wild and may have increased disease susceptibility and reduced immune function. Eastern hellbenders (Cryptobranchus alleganiensis alleganiensis) have suffered precipitous population declines over the past few decades. To bolster populations, eastern hellbenders are reared in captivity before being translocated to the wild. However, the absence of natural microbial reservoirs within the captive environment diminishes the diversity of skin-associated bacteria on hellbender skin and may negatively influence their ability to defend against pathogenic species once they are released into the wild. To prepare hellbenders for natural bacteria found in riverine environments, we devised a novel bioaugmentation method to increase the diversity of skin microbial communities within a captive setting. We exposed juvenile hellbenders to increasing amounts of river water over 5 weeks before translocating them to the river. We genetically identified and phylogenetically compared bacteria collected from skin swabs and river water for alpha (community richness) and beta (community composition) diversity estimates. We found that hellbenders exposed to undiluted river water in captivity had higher alpha diversity and distinct differentiation in the community composition on their skin, compared to hellbenders only exposed to well water. We also found strong evidence that hellbender skin microbiota is host-specific rather than environmentally driven and is colonized by rare environmental operational taxonomic units in river water. This technique may increase hellbender translocation success as increasing microbial diversity is often correlated with elevated disease resistance. Future work is necessary to refine our methods, investigate the relationship between microbial diversity and hellbender health and understand how this bioaugmentation technique influences hellbenders’ survival following translocation from captivity into the wild

Edge detection algorithm based on fuzzy logic theory for a local vision system of robocup humanoid league

Este artículo presenta el desarrollo de un algoritmo para la extracción de bordes basado en la teoría de lógica difusa, mediante el cual es posible reconocer las marcas de un campo de juego para la liga humanoide de RoboCup. El algoritmo propuesto describe la creación de un sistema de inferencia difuso que permite evaluar la relación existente entre los pixeles de una imagen, encontrando así las variaciones en los niveles de gris para una vecindad de pixeles específica. Posteriormente se plantea la implementación del método OTSU para binarizar la imagen obtenida en el proceso difuso y así generar una imagen que contiene solo los bordes extraídos, validando el algoritmo en imágenes de la liga humanoide. Luego se analizan los resultados obtenidos evidenciando el buen desempeño del algoritmo, considerando que el tiempo de procesamiento de la propuesta es tan solo el 35% más del tiempo necesario en métodos tradicionales, mientras que los bordes extraídos son unos 52% menos susceptibles al ruido.At this paper we shown the development of an algorithm to perform edges extraction based on fuzzy logic theory. This method allows recognizing landmarks on the game field for Humanoid League of RoboCup. The proposed algorithm describes the creation of a fuzzy inference system that permit evaluate the existent relationship between image pixels, finding variations on grey levels of related neighbor pixels. Subsequently, it shows an implementation of OTSU method to binarize an image that was obtained from fuzzy process and so generate an imagecontaining only extracted edges, validating the algorithm with Humanoid League images. Later, we analyze obtained results that evidence a good performance of algorithm, considering that this proposal only takes an extra 35% processing time that will be required by traditional methods, whereas extracted edges are 52% less noise susceptible

Worldsheet Scattering in AdS_5 x S^5

We calculate the S-matrix in the gauge-fixed sigma-model on AdS_5 x S^5 to the leading order in perturbation theory, and analyze how supersymmetry is realized on the scattering states. A mild nonlocality of the supercharges implies that their action on multi-particle states does not follow the Leibniz rule, which is replaced by a nontrivial coproduct. The plane wave symmetry algebra is thus naturally enhanced to a Hopf algebra. The scattering matrix elements obey the classical Yang-Baxter equation modified by the existence of the coproduct. This structure mirrors that of the large 't Hooft coupling expansion of the S-matrix for the spin chain in the dual super-Yang-Mills theory.Comment: 51 pages, v2: references added, v3: sign in (2.12), (6.19) and (6.21) corrected; v4: discussion of classical YBE is considerably modifie

EUROnu-WP6 2010 Report

This is a summary of the work done by the Working Package 6 (Physics) of the EU project "EUROnu" during the second year of activity of the project.Comment: 82 pages, 51 eps figure

Epi-and Mesopelagic Fishes, Acoustic Data, and SST Images Collected Off Lanzarote, Fuerteventura, and Gran Canaria, Canary Islands, During Cruise La Bocaina 04-97

During cruise La Bocaina 0497 a series of 14 tows with a commercial pelagic trawl at depths between 20 and 700 m and an acoustic survey with a SIMRAD EK-500 echosounder were carried out in neritic and adjacent oceanic waters off Lanzarote, Fuerteventura, and Gran Canaria, Canary islands. In addition, SST images were obtained to get some informations on the prevailing hydrological conditions in the study area. Of the 14 trawl tows ten were successful and resulted in capture of a total of 2166 fishes belonging to 81 species, 53 genera and 28 families. Ten species proved to be new records for the area of the Canary Islands. The acoustic survey covered a total of 2404 nm2 and allowed to obtain estimates of the abundance and biomass of fishes. Both the fishing results and the acoustic study revealed considerable spatial variability in biomass of chub mackerel, Scomber japonicus, an epipelagic species of particular fisheries interest, showing clear variations in the numerical dominance of certain age- or size classes among different collecting localities. Furthermore, some juveniles in the first year of life occurred in the oceanic epipelagic during night together with vertically migrating mesopelagic fishes. Preliminary analyses of the stomach and intestinal content suggest that these juveniles fed close to the bottom during the day before and hence may undertake diurnal horizontal migrations between the shelf and adjacent oceanic waters. It is concluded that the great spatial flexibility of this species requires further monitoring surveys at regular intervals as a basis for establishing a sustainable fisheries management. The acoustic data together with the trawling results demonstrate the existence of a dense deep scattering layer at depths between 400 and 700 m which is composed of mesopelagic fishes and invertebrates. Clear indications of diurnal vertical migrations were found in several species with formation of a shallow scattering layer in less than 150 m depth during night. Together with typical mesopelagic species such as stomiids and myctophids also juveniles of three benthopelagic species, which typically undergo a pelagic stage during early life, were collected. Several individuals of mesopelagic species were collected above the shelf what may reflect the ecological boundary situation at ocean rims typical for oceanic islands and seamounts. Some mesopelagic species collected in this study may be closely associated with distinct hydrological conditions such as the myctophid Ceratoscopelus maderensis which was discovered in the SE of Fuerteventura in an area of possible local upwelling. This finding points to the existence of micro-zoogeographic zones within the Canary islands which derserve further investigation using a small-scaled comparative approach

Quality Indicators for the Diagnosis and Management of Primary Hyperparathyroidism

IMPORTANCE Primary hyperparathyroidism (pHPT) is a common endocrine disorder with many diagnostic and treatment challenges. Despite high-quality guidelines, care is variable, and there is low adherence to evidence-based treatment pathways. OBJECTIVE To develop quality indicators (QIs) to evaluate the diagnosis and treatment of pHPT that could measure, improve, and optimize quality of care and outcomes for patients with this disease. DESIGN, SETTING, AND PARTICIPANTS This quality improvement study used a guideline-based approach to develop QIs that were ranked by a Canadian 9-member expert panel of 3 endocrinologists, 3 otolaryngologists, and 3 endocrine surgeons. Data were analyzed between September 2020 and May 2021. MAIN OUTCOMES AND MEASURES Candidate indicators (CIs) were extracted from published primary hyperparathyroidism guidelines and summarized with supporting evidence. The 9-member expert panel rated each CI on the validity, reliability, and feasibility of measurement. Final QIs were selected from CIs using the modified RAND-University of California, Los Angeles appropriateness methodology. All panelists were then asked to rank the top 5 QIs for primary, endocrine, and surgical care. RESULTS Forty QIs were identified and evaluated by the expert panel. After 2 rounds of evaluations and discussion, a total of 18 QIs were selected as appropriate measures of high-quality care. The top 5 QIs for primary, endocrine, and surgical care were selected following panelist rankings. CONCLUSIONS AND RELEVANCE This quality improvement study proposes 18 QIs for the diagnosis and management of pHPT. Furthermore, the top 5 QIs applicable to physicians commonly treating pHPT, including general physicians, internists, endocrinologists, otolaryngologists, and surgeons, are included. These QIs not only assess the quality of care to guide the process of improvement, but also can assess the implementation of evidence-based guideline recommendations. Using these indicators in clinical practice and health system registries can improve quality and cost-effectiveness of care for patients with pHPT

Does abscisic acid affect strigolactone biosynthesis?

Strigolactones are considered a novel class of plant hormones that, in addition to their endogenous signalling function, are exuded into the rhizosphere acting as a signal to stimulate hyphal branching of arbuscular mycorrhizal (AM) fungi and germination of root parasitic plant seeds. Considering the importance of the strigolactones and their biosynthetic origin (from carotenoids), we investigated the relationship with the plant hormone abscisic acid (ABA). Strigolactone production and ABA content in the presence of specific inhibitors of oxidative carotenoid cleavage enzymes and in several tomato ABA-deficient mutants were analysed by LC-MS/MS. In addition, the expression of two genes involved in strigolactone biosynthesis was studied. * • The carotenoid cleavage dioxygenase (CCD) inhibitor D2 reduced strigolactone but not ABA content of roots. However, in abamineSG-treated plants, an inhibitor of 9-cis-epoxycarotenoid dioxygenase (NCED), and the ABA mutants notabilis, sitiens and flacca, ABA and strigolactones were greatly reduced. The reduction in strigolactone production correlated with the downregulation of LeCCD7 and LeCCD8 genes in all three mutants. * • The results show a correlation between ABA levels and strigolactone production, and suggest a role for ABA in the regulation of strigolactone biosynthesis

The History of Galaxy Formation in Groups: An Observational Perspective

We present a pedagogical review on the formation and evolution of galaxies in groups, utilizing observational information from the Local Group to galaxies at z~6. The majority of galaxies in the nearby universe are found in groups, and galaxies at all redshifts up to z~6 tend to cluster on the scale of nearby groups (~1 Mpc). This suggests that the group environment may play a role in the formation of most galaxies. The Local Group, and other nearby groups, display a diversity in star formation and morphological properties that puts limits on how, and when, galaxies in groups formed. Effects that depend on an intragroup medium, such as ram-pressure and strangulation, are likely not major mechanisms driving group galaxy evolution. Simple dynamical friction arguments however show that galaxy mergers should be common, and a dominant process for driving evolution. While mergers between L_* galaxies are observed to be rare at z < 1, they are much more common at earlier times. This is due to the increased density of the universe, and to the fact that high mass galaxies are highly clustered on the scale of groups. We furthermore discus why the local number density environment of galaxies strongly correlates with galaxy properties, and why the group environment may be the preferred method for establishing the relationship between properties of galaxies and their local density.Comment: Invited review, 16 pages, to be published in ESO Astrophysics Symposia: "Groups of Galaxies in the Nearby Universe", eds. I. Saviane, V. Ivanov, J. Borissov

Foundations of the AdS_5 x S^5 Superstring. Part I

We review the recent advances towards finding the spectrum of the AdS_5 x S^5 superstring. We thoroughly explain the theoretical techniques which should be useful for the ultimate solution of the spectral problem. In certain cases our exposition is original and cannot be found in the existing literature. The present Part I deals with foundations of classical string theory in AdS_5 x S^5, light-cone perturbative quantization and derivation of the exact light-cone world-sheet scattering matrix.Comment: 161 page