Minat Kaum Muda Dayak Mualang dalam Memainkan Alat Musik Tradisional Tebah Genang Manang Brani

This reseach is conducted due to the lack of interest of young people the Dayaknese Mualang traditional music and there is a tendency of the elder are more involved to play the traditional music, such as Tebah Genang Manang Brani. Tebah Genang Manang Brani music is a tradition of Dayak Mualang tribe which is inherited from ancestors of Dayak Mualang from generation to generation. The aim this reseach was: 1) to describe the interest of youngsters of Dayak Mualang in playing traditional music. 2) to describe the different of rhythm pattern between the elders and youngsters in playing traditional music Tebah Genang Manang Brani of Dayak Mualang in Sekadau regency. The method of the research that was used is survey. All of the research is the fact about Tebah Genang Manang Brani. The data was analized by qualitative method with the sources such as Edmundus Linggi, Maksimus, the youth of Dayaknese Mualang and the other artists who were highly involved and knew more about Traditional Music Tebah Genang Manang Brani. The data was collected through interview, observation, documentation, and questionnaires

Bromatological and organoleptic quality of organic waste silages of coffee-musaceous production system

P?ginas 47-52El presente estudio se realiz? en el Laboratorio de Ecofisiolog?a Animal de la Universidad del Tolima (Ibagu?, Colombia). El objetivo fue analizar la calidad nutricional mediante las caracter?sticas bromatol?gicas y organol?pticas de ensilajes de residuos org?nicos del agroecosistema caf? y mus?ceas. Se prepar? un ensilaje con cada uno de los residuos org?nicos (caf?, hoja de pl?tano y v?stago de pl?tano), se le adiciono 5% de melaza como fuente energ?tica. Las unidades de an?lisis estaban representadas por microsilos de 5 Kg empacadas en bolsa de polietileno calibre 6. Se evaluaron 4 tiempos de fermentaci?n (1, 7, 14 y 21 d?as). Semanalmente se evaluaron las siguientes variables: pH, temperatura, materia seca, cenizas. Al final del proceso se evalu? el porcentaje de prote?na, FDN, FDA y Lignina. Los resultados obtenidos muestran que la temperatura m?xima que se registr? al inicio fue de 26 ?C y se estabilizo a los 23,3 ?C a trav?s del tiempo, el valor m?s bajo de pH registrado fue el ensilaje de cereza de caf? (3,8), el ensilaje de hoja de pl?tano en el tiempo 4 de fermentaci?n presento el porcentaje de prote?na m?s alto (14,45%), seguido de los ensilajes de cereza de caf? (11,65%) y v?stago de pl?tano (5,25%). En la evaluaci?n organol?ptica se realiz? un an?lisis mediante estad?stica descriptiva donde se evidenci? que todos los ensilajes presentaron caracter?sticas aceptables dentro de los par?metros de calidad en las caracter?sticas de color, olor, humedad y textura.ABSTRACT. This study was carried out in the Laboratory of Animal Ecophysiology, University of Tolima (Ibague, Colombia). The objective was to analyze the nutritional quality through bromatological and organoleptic characteristics of organic waste silages of coffee and musaceous agroecosystem. Silages were prepared with each of the organic wastes (coffee, banana leaves and banana stems) 5% molasses was added as an energy source. The units of analysis were represented by 5 kg microsilos packed in 6 gauge polyethylene bags. 4 fermentation times were evaluated (1, 7, 14 and 21 days). The following variables were evaluated weekly: pH, temperature, dry matter, ash. At the end of the process the percentage of protein, NDF, and lignin FDA were evaluated. The results show that the highest temperature recorded at the beginning was 26 ? C and stabilized at 23,3 ?C over time, the lowest value reported was the silage pH of coffee (3,8), the banana leaf silage on fermentation time 4 presented the highest protein content (14,45 %), followed by coffee silages (11,65%) and banana stem (5,25%). The sensory evaluation was analyzed using descriptive statistics which showed that all silages showed characteristics within acceptable quality parameters on the characteristics of color, smell, humidity and texture

Reduction of nucleosome assembly during new DNA synthesis impairs both major pathways of double-strand break repair

Assembly of new chromatin during S phase requires the histone chaperone complexes CAF-1 (Cac2p, Msi1p and Rlf2p) and RCAF (Asf1p plus acetylated histones H3 and H4). Cells lacking CAF-1 and RCAF are hypersensitive to DNA-damaging agents, such as methyl methanesulfonate and camptothecin, suggesting a possible defect in double-strand break (DSB) repair. Assays developed to quantitate repair of defined, cohesive-ended break structures revealed that DSB-induced plasmid:chromosome recombination was reduced ∼10-fold in RCAF/CAF-1 double mutants. Recombination defects were similar with both chromosomal and plasmid targets in vivo, suggesting that inhibitory chromatin structures were not involved. Consistent with these observations, ionizing radiation-induced loss of heterozygosity was abolished in the mutants. Nonhomologous end-joining (NHEJ) repair proficiency and accuracy were intermediate between wild-type levels and those of NHEJ-deficient yku70 and rad50 mutants. The defects in NHEJ, but not homologous recombination, could be rescued by deletion of HMR-a1, a component of the a1/alpha2 transcriptional repressor complex. The findings are consistent with the observation that silent mating loci are partially derepressed. These results demonstrate that defective assembly of nucleosomes during new DNA synthesis compromises each of the known pathways of DSB repair and that the effects can be indirect consequences of changes in silenced chromatin structure

Control of Centrin Stability by Aurora A

Aurora A is an oncogenic serine/threonine kinase which can cause cell transformation and centrosome amplification when over-expressed. Human breast tumors show excess Aurora A and phospho-centrin in amplified centrosomes. Here, we show that Aurora A mediates the phosphorylation of and localizes with centrin at the centrosome, with both proteins reaching maximum abundance from prophase through metaphase, followed by their precipitous loss in late stages of mitosis. Over-expression of Aurora A results in excess phospho-centrin and centrosome amplification. In contrast, centrosome amplification is not seen in cells over-expressing Aurora A in the presence of a recombinant centrin mutant lacking the serine phosphorylation site at residue 170. Expression of a kinase dead Aurora A results in a decrease in mitotic index and abrogation of centrin phosphorylation. Finally, a recombinant centrin mutation that mimics centrin phosphorylation increases centrin's stability against APC/C-mediated proteasomal degradation. Taken together, these results suggest that the stability of centrin is regulated in part by Aurora A, and that excess phosphorylated centrin may promote centrosome amplification in cancer

Overexpression of the aphid-induced serine protease inhibitor <i>CI2c </i>gene in barley affects the generalist green peach aphid, not the specialist bird cherry-oat aphid

<div><p>Aphids are serious pests in crop plants. In an effort to identify plant genes controlling resistance against aphids, we have here studied a protease inhibitor, CI2c in barley (<i>Hordeum vulgare</i> L.). The <i>CI2c</i> gene was earlier shown to be upregulated by herbivory of the bird cherry-oat aphid <i>(Rhopalosiphum padi</i> L.<i>)</i> in barley genotypes with moderate resistance against this aphid, but not in susceptible lines. We hypothesized that CI2c contributes to the resistance. To test this idea, cDNA encoding <i>CI2c</i> was overexpressed in barley and bioassays were carried out with <i>R</i>. <i>padi</i>. For comparison, tests were carried out with the green peach aphid (<i>Myzus persicae</i> Sulzer), for which barley is a poor host. The performance of <i>R</i>. <i>padi</i> was not different on the <i>CI2c</i>-overexpressing lines in comparison to controls in test monitoring behavior and fecundity. <i>M</i>. <i>persicae</i> preference was affected as shown in the choice test, this species moved away from control plants, but remained on the <i>CI2c</i>-overexpressing lines. <i>R</i>. <i>padi</i>-induced responses related to defense were repressed in the overexpressing lines as compared to in control plants or the moderately resistant genotypes. A putative susceptibility gene, coding for a β-1,3-glucanase was more strongly induced by aphids in one of the <i>CI2c</i>-overexpressing lines. The results indicate that the CI2c inhibitor in overexpressing lines affects aphid-induced responses by suppressing defense. This is of little consequence to the specialist <i>R</i>.<i>padi</i>, but causes lower non-host resistance towards the generalist <i>M</i>. <i>persicae</i> in barley.</p></div

TOI-969: a late-K dwarf with a hot mini-Neptune in the desert and an eccentric cold Jupiter

Context. The current architecture of a given multi-planetary system is a key fingerprint of its past formation and dynamical evolution history. Long-term follow-up observations are key to complete their picture. Aims. In this paper, we focus on the confirmation and characterization of the components of the TOI-969 planetary system, where TESS detected a Neptune-size planet candidate in a very close-in orbit around a late K-dwarf star. Methods. We use a set of precise radial velocity observations from HARPS, PFS, and CORALIE instruments covering more than two years in combination with the TESS photometric light curve and other ground-based follow-up observations to confirm and characterize the components of this planetary system. Results. We find that TOI-969 b is a transiting close-in (Pb ∼ 1.82 days) mini-Neptune planet (Formula Presented), placing it on the lower boundary of the hot-Neptune desert (Teq,b = 941 \ub1 31 K). The analysis of its internal structure shows that TOI-969 b is a volatile-rich planet, suggesting it underwent an inward migration. The radial velocity model also favors the presence of a second massive body in the system, TOI-969 c, with a long period of (Formula Presented) days, a minimum mass of (Formula Presented), and a highly eccentric orbit of (Formula Presented). Conclusions. The TOI-969 planetary system is one of the few around K-dwarfs known to have this extended configuration going from a very close-in planet to a wide-separation gaseous giant. TOI-969 b has a transmission spectroscopy metric of 93 and orbits a moderately bright (G = 11.3 mag) star, making it an excellent target for atmospheric studies. The architecture of this planetary system can also provide valuable information about migration and formation of planetary systems

Philodryas chamissonis (Reptilia: Squamata: Colubridae) preys on the arboreal marsupial Dromiciops gliroides (Mammalia: Microbiotheria: Microbiotheriidae)

Philodryas chamissonis, the Chilean long-tailed snake, is a diurnal predator mainly of Liolaemus lizards, but also of amphibians, birds, rodents and juvenile rabbits. Dromiciops gliroides (Colocolo opossum) is an arboreal marsupial endemic of temperate rainforest of southern South America. Little information is available about this marsupial's biology and ecology. Here we report the predation of one Colocolo opossum by an adult female P. chamissonis in a mixed Nothofagus forest, composed mainly by N. dombeyi, N. glauca and N. alpina trees, in the "Huemules de Niblinto" National Reserve, Nevados de Chillán, Chile. Since these two species have different activity and habitat use patterns, we discuss how this encounter may have occurred. Although it could just have been an opportunistic event, this finding provides insights into the different components of food chains in forest ecosystems of Chile

Autopsy of an eruptive phase of Tungurahua volcano (Ecuador) through coupling of seismo-acoustic and SO 2 recordings with ash characteristics

Eruption style and dynamics are controlled by various parameters including magma supply rate, magma viscosity, volatile content, and the permeability of the conduit. Rapid changes of these parameters can significantly modify the hazards associated to the eruption processes and understanding their relationship with multiparametric geophysical monitoring data can greatly improve our forecasting capacities. From 2008 to 2016, volcanic activity at Tungurahua was characterized by eruptive phases separated by episodes of quiescence. These phases displayed great variability of eruptive patterns including Vulcanian and Strombolian explosions, low pyroclastic fountaining, continuous or sporadic ash emissions and passive degassing. We use the comparison between geophysical data (seismic, acoustic and SO 2 emission), recorded by permanent monitoring networks, and the characteristics of the emitted ash to track changes in eruption dynamics during an eruptive phase that lasted from late December 2009 to March 2010. We show that the correlation between the analyzed parameters allows imaging and interpretation of the conditions at the vent. At Tungurahua, these conditions can rapidly change at the time scale of a single eruptive phase, corresponding to various degrees of opening, plugging and permeability of the conduit. Two magma intrusions could be identified during a single eruptive phase showing transitions between violent Strombolian and Vulcanian activity. Changes in the componentry of the analyzed ash samples, together with the geophysical data, nicely highlight these evolutions. Studying these parameters simultaneously provides a unique insight into the physical processes controlling superficial volcanic activity and offers a potential tool for better understanding volcanoes and detecting changes in their activity. The joint interpretation of multiparametric data which we propose is potentially applicable to multiple andesitic volcanoes