Deep Sclerectomy With a New Nonabsorbable Uveoscleral Implant (Esnoper-Clip) : 1-Year Outcomes

Altres ajuts: AJL Ophthalmics supports the Health Sciences Research Institute "Germans Trias i Pujol" Foundation.520 _

Analysis of product shrinkage and waste in a potato bagging plant

Nowadays, an average of 2 kg of waste per person are generated in Spain. Furthermore, the household consumption is rising and, as a consequence, the waste production is also increasing. This trend presents a direct impact in the environment. Moreover, after two years of COVID-19 pandemic, it has been detected a stronger rise in consumption per person, while consumption through professional commercial channels for hospitality industry has been lower. This paper analizes the waste generation and product shrinkage in a potato bagging plant, which addresses its production to both final consumers and retailers. The raw materials washing line, as well as the production line, are taken into consideration in the analysis, while new uses to the produced waste are proposed, aiming at providing new useful life, such as the production of bioplastics or the production of biodiesel. As a consequence, the environment impact is minimized and new products are obtained

Role of GUCA1C in Primary Congenital Glaucoma and in the Retina: Functional Evaluation in Zebrafish

Primary congenital glaucoma (PCG) is a heterogeneous, inherited, and severe optical neuropathy caused by apoptotic degeneration of the retinal ganglion cell layer. Whole-exome sequencing analysis of one PCG family identified two affected siblings who carried a low-frequency homozygous nonsense GUCA1C variant (c.52G > T/p.Glu18Ter/rs143174402). This gene encodes GCAP3, a member of the guanylate cyclase activating protein family, involved in phototransduction and with a potential role in intraocular pressure regulation. Segregation analysis supported the notion that the variant was coinherited with the disease in an autosomal recessive fashion. GCAP3 was detected immunohistochemically in the adult human ocular ciliary epithelium and retina. To evaluate the ocular effect of GUCA1C loss-of-function, a guca1c knockout zebrafish line was generated by CRISPR/Cas9 genome editing. Immunohistochemistry demonstrated the presence of GCAP3 in the non-pigmented ciliary epithelium and retina of adult wild-type fishes. Knockout animals presented up-regulation of the glial fibrillary acidic protein in Müller cells and evidence of retinal ganglion cell apoptosis, indicating the existence of gliosis and glaucoma-like retinal damage. In summary, our data provide evidence for the role of GUCA1C as a candidate gene in PCG and offer new insights into the function of this gene in the ocular anterior segment and the retina.This research was funded by research grants from the “Instituto de Salud Carlos III/European Regional Development Fund (ERDF)” (PI15/01193, PI19/00208 and RD16/0008/0019, OFTARED), the Regional Ministry of Science and Technology of the Board of the Communities of “Castilla-La Mancha” (SBPLY/17/180501/000404; http://www.educa.jccm.es/idiuniv/es). SA-M was sponsored by the Regional Ministry of Science and Technology of the Board of the Communities of “Castilla-La Mancha” (PREJCCM2016/28)

Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats

Introduction. Ischemia/reperfusion (IR) injury, often associated with liver surgery, is an unresolved problem in the clinical practice. Spironolactone is an antagonist of aldosterone that has shown benefits over IR injury in several tissues, but its effects in hepatic IR are unknown. Objective. To evaluate the effect of spironolactone on IR-induced damage in liver. Materials and Methods. Total hepatic ischemia was induced in rats for 20 min followed by 60 min of reperfusion. Spironolactone was administered and hepatic injury, cytokine production, and oxidative stress were assessed. Results. After IR, increased transaminases levels and widespread acute inflammatory infiltrate, disorganization of hepatic hemorrhage trabeculae, and presence of apoptotic bodies were observed. Administration of SPI reduced biochemical and histological parameters of liver injury. SPI treatment increased IL-6 levels when compared with IR group but did not modify either IL-1β or TNF-α with respect to IR group. Regarding oxidative stress, increased levels of catalase activity were recorded in IR + SPI group in comparison with group without treatment, whereas MDA levels were similar in IR + SPI and IR groups. Conclusions. Spironolactone reduced the liver damage induced by IR, and this was associated with an increase in IL-6 production and catalase activity

Study of USH1 Splicing Variants through Minigenes and Transcript Analysis from Nasal Epithelial Cells

Usher syndrome type I (USH1) is an autosomal recessive disorder characterized by congenital profound deafness, vestibular areflexia and prepubertal retinitis pigmentosa. The first purpose of this study was to determine the pathologic nature of eighteen USH1 putative splicing variants found in our series and their effect in the splicing process by minigene assays. These variants were selected according to bioinformatic analysis. The second aim was to analyze the USH1 transcripts, obtained from nasal epithelial cells samples of our patients, in order to corroborate the observed effect of mutations by minigenes in patient’s tissues. The last objective was to evaluate the nasal ciliary beat frequency in patients with USH1 and compare it with control subjects. In silico analysis were performed using four bioinformatic programs: NNSplice, Human Splicing Finder, NetGene2 and Spliceview. Afterward, minigenes based on the pSPL3 vector were used to investigate the implication of selected changes in the mRNA processing. To observe the effect of mutations in the patient’s tissues, RNA was extracted from nasal epithelial cells and RT-PCR analyses were performed. Four MYO7A (c.470G>A, c.1342_1343delAG, c.5856G>A and c.3652G>A), three CDH23 (c.2289+1G>A, c.6049G>A and c.8722+1delG) and one PCDH15 (c.3717+2dupTT) variants were observed to affect the splicing process by minigene assays and/or transcripts analysis obtained from nasal cells. Based on our results, minigenes are a good approach to determine the implication of identified variants in the mRNA processing, and the analysis of RNA obtained from nasal epithelial cells is an alternative method to discriminate neutral Usher variants from those with a pathogenic effect on the splicing process. In addition, we could observe that the nasal ciliated epithelium of USH1 patients shows a lower ciliary beat frequency than control subjects

Influence of the Cumulative Incidence of COVID-19 Cases on the Mental Health of the Spanish Out-of-Hospital Professionals

This study aimed to analyze the psychological affectation of health professionals (HPs) of Spanish Emergency Medical Services (EMSs) according to the cumulative incidence (CI) of COVID19 cases in the regions in which they worked. A cross-sectional descriptive study was designed, including all HPs working in any EMS of the Spanish geography between 1 February 2021 and 30 April 2021. Their level of stress, anxiety and depression (DASS-21) and the perception of self-efficacy (GSES) were the study’s main results. A 2-factor analysis of covariance was used to determine if the CI regions of COVID-19 cases determined the psychological impact on each of the studied variables. A total of 1710 HPs were included. A third presented psychological impairment classified as severe. The interaction of CI regions with the studied variables did not influence their levels of stress, anxiety, depression or self-efficacy. Women, younger HPs or those with less EMS work experience, emergency medical technicians (EMT), workers who had to modify their working conditions or those who lived with minors or dependents suffered a greater impact from the COVID-19 pandemic in certain regions. These HPs have shown high levels of stress, anxiety, depression and medium levels of self-efficacy, with similar data in the different geographical areas. Psychological support is essential to mitigate their suffering and teach them to react to adverse events.This research was funded by Fundación ASISA and Sociedad Española de Urgencias y Emergencias (SEMES)

Medicine, cinema and literature: A teaching experiment at the Universitat Autónoma de Barcelona

[ES] Es una percepción común que los estudiantes de medicina pasan los seis años de licenciatura obsesionados con el examen MIR y, una vez superado, el sistema sanitario y la excesiva actividad asistencial fomenta la hiperespecialización, lo que en muchas ocasiones es en detrimento de aprender a leer al enfermo como un todo. El ejercicio de la profesión debería entender al paciente en su globalidad, ya que la medicina entendida como un humanismo ético, fracasa si se especializa al paciente. En la Unidad Docente del Hospital UniversitariGermans Trias i Pujol de la Universitat Autónoma de Barcelona nos planteamos incorporar una asignatura que permitiera a los alumnos leer y ver la medicina mucho más allá del diagnóstico, que les posibilitara una visión más amplia de la enfermedad y que se convirtiera en el complemento emocional de su licenciatura (y, en consecuencia directa, de una profesión) excesivamente biologista. ¿Qué mejor que la literatura y el cine para enseñar a los alumnos a leer y ver la medicina ? Por el conocimiento que tenemos de otros programas universitarios se trata no sólo de una asignatura novedosa, sino de un modelo de aprendizaje absolutamente diferente a los habituales en esta licenciatura, no así en otros estudios, como los de gestión empresarial, donde sí suele trabajarse con asignaturas y modelos similares.[EN] In Spain, it is commonly held that medical students spend the entire time of their degree courses obsessed with the MIR (Post-graduate Medical Training/ Medical Residency) exam and, when they have sat and passed this, the health system itself together with excessive professional activity foster what is tantamount to a hyperspecialisation , in many cases in detriment to learning to read the patient as a complete entity. Upon exercising their profession, physicians should understand patients in their entirety since medicine when considered as ethical humanism- fails if the patient is merely slotted into the specialist pigeon-hole. At the Teaching Unit of the Hospital Universitari Germans Trias i Pujol of the Universitat Autónoma de Barcelona we were prompted to incorporate a subject in the syllabus that would allow students to both read and see medicine to an extent far beyond the diagnostic level, that would provide them with a broader view of diseases, and one that that would become an emotional complement to the material studied in their degree course (and as a direct consequence, to their future profession), which tends to be excessively biologistic . What, then, could be better than literature and the cinema to teach studentsto read and see medicine ? From previous knowledge that we had culled from other university programs, this would not only be a novel subject per se but should also offer a completely different learning model from traditional teaching contents in the field. This would thus approach the situation in other degree courses, such as in business studies, where similar subjects and models are used

Descripción y vistas del hotel "Villa Bilbao" propiedad del arquitecto don Julio de Saracibar

Ex-libris de Mariano Rodríguez de Riva

The histidine-phosphocarrier protein of the phosphoenolpyruvate: sugar phosphotransferase system of bacillus sphaericus self-associates

15 pags, 7 figs, 1 tabThe phosphotransferase system (PTS) is involved in the use of carbon sources in bacteria. Bacillus sphaericus, a bacterium with the ability to produce insecticidal proteins, is unable to use hexoses and pentoses as the sole carbon source, but it has ptsHI genes encoding the two general proteins of the PTS: enzyme I (EI) and the histidine phosphocarrier (HPr). In this work, we describe the biophysical and structural properties of HPr from B. sphaericus, HPrbs, and its affinity towards EI of other species to find out whether there is inter-species binding. Conversely to what happens to other members of the HPr family, HPrbs forms several self-associated species. The conformational stability of the protein is low, and it unfolds irreversibly during heating. The protein binds to the N-terminal domain of EI from Streptomyces coelicolor, EINsc, with a higher affinity than that of the natural partner of EINsc, HPrsc. Modelling of the complex between EINsc and HPrbs suggests that binding occurs similarly to that observed in other HPr species. We discuss the functional implications of the oligomeric states of HPrbs for the glycolytic activity of B. sphaericus, as well as a strategy to inhibit binding between HPrsc and EINsc. © 2013 Doménech et al.This work was supported by the Spanish Ministerio de Ciencia e Innovación (MCINN) (CTQ2011-24393, and CSD2008-00005 to JLN; BIO2009-13261-C02- 01/02 and P09-CVI-5063, with Fondo Social Europeo (ESF) to ACA; and BFU2010-19451 to AVC), Diputación General de Aragón (PI044/09 to AVC), intramural BIFI 2011 projects (to AVC and JLN), Junta de Andalucía (BIO-328 to ACA), and by grants from the Agencia Nacional de Promoción Científica y Tecnológica Argentina (ANPCyT; PICT-2011-2778) (to CNC). SMR was supported by the CSD2008-00005. The stays of RD in the laboratory of AVC were supported by the Spanish Ministerio de Ciencia e Innovación (BFU2008-02302-BMC)