Dental implants rehabilitation in a patient with head and neck radiotherapy for osteosarcoma in the jaw. A clinical case report

A 52-year-old female patient with a diagnostic of osteosarcoma in the mandible, in which it was necessary a reconstruction with a microvascularized osteomyocutaneous fibula bone. Coadjuvant chemotherapy was scheduled. Two years later, 4 osseointegrated implants (OII) were placed in the fibula a 2 OII in the right mandible, using a splint guided surgery. The final prosthodontic consisted in a metal ceramic restoration using CAD/ CAM technology

Central venous-catheter related bacteremia: incidence and risk factors in a hospital in western Mexico

AbstractBackgroundCentral venous catheters (CVC) are needed for monitoring and treatment of critically ill patients; however, their use increases the risk of bacteremia. The aim of the study was to quantify the incidence of central venous catheter-related bacteremia (CVCRB) and to identify factors associated with this infection.MethodsA prospective cohort study was conducted in a concentration hospital of western Mexico. The association of CVCRB and study variables was investigated using multivariate Cox regression analysis.ResultsTwo hundred and four patients with CVC were studied. The mean age was 4.6 years; 66.2% were male. Insertion sites of the catheters were subclavian vein 72.5% (n = 148), jugular vein 20.1% (n = 41) and femoral vein 7.4% (n = 15). CVCRB incidence was 6.5 events/1,000 catheter-days; microorganisms identified were gram-positive cocci 37.5% (n = 6), gram-negative bacilli 37.5% (n = 6) and Candida albicans 25% (n = 4). It was observed that the increase in catheter manipulations per day was associated with bacteremia (HR 1.14, 95% CI 1.06–1.23), whereas the use of intravenous antibiotics showed a protective effect (HR 0.84, 95% CI 0.76–0.92).ConclusionsIn addition to the strategies of maximum caution when placing or manipulating the catheter, we recommend decreasing, as much as possible, disconnects between the CVC and the infusion line. Antibiotics showed a protective effect, but the outcome is uncertain and the promotion of antimicrobial resistance should be considered

Antibodies to endothelial cells in Behçet's disease: cell-binding heterogeneity and association with clinical activity

OBJECTIVES--To investigate the prevalence and characteristics of antibodies to endothelial cells (aEC) from large vessel and from microvasculature in a group of patients with Behçet's disease (BD) to determine the relationship of these antibodies with clinical and laboratory features of the disease. METHODS--Thirty patients with BD were prospectively and consecutively studied. The aEC were determined by enzyme-linked immunosorbent assay (ELISA) using endothelial cells derived from human umbilical vein (large vessel) as well as from retroperitoneal adipose tissue (microvasculature). RESULTS--Fifteen patients (50%) had aEC, either directed to large vessel [8(26%) patients] or microvascular [13(43%) patients] endothelial cells. The percentage of active patients was significantly higher in the aEC-positive group [12(80%) patients] compared with the aEC-negative group [5(33%) patients] (p < 0.05). CONCLUSIONS--Patients with BD have a high prevalence of aEC when microvascular endothelial cells are used in the assay. These antibodies seem to be a marker of disease activity in this condition, previously considered as negative for autoantibodies

Camptothecin induces the transit of fASl trimers to the cell surface in apoptotic heP-2 cells

Fas ligand (L) is a membrane protein from the tumor necrosis factor (TNF) family. It induces apoptosis upon contact with its Fas/CD95/APO1 receptor. Trimerization of FasL on the surface of effector cells is essential in the binding of the Fas trimer of the target cells. The receptor then recruits an adaptor and caspase-like proteins which lead apoptosis. This paper reports on the fate of FasL in HEp-2 cells committed to apoptosis by induction with campthotecin. Our main results demonstrated that in non-apoptotic cells, FasL aggregates in the cytoplasm forming trimers of 120 kDa. Apoptosis increases the trimeric FasL species, but also induces its dissociation into monomers of 35 kDa. In conclusion, camptothecin appears to perturb the Fas and FasL segregation in the cytoplasm by promoting the transit of FasL to the cell surface, thus fostering a process of autocrine or paracrine apoptosis. FasL is trimerized prior to Fas/FasL complex formation, and after apoptosis, FasL undergoes an intense turnover

Atomic surface segregation and structural characterization of PdPt bimetallic nanoparticles

"Bimetallic nanoparticles are of interest since they lead to many interesting electrical, chemical, catalytic, and optical properties. They are particularly important in the field of catalysis since they show superior catalytic properties than their monometallic counterparts. The structures of bimetallic nanoparticles depend mainly on the synthesis conditions and the miscibility of the two components. In this work, PdPt alloyed-bimetallic nanoparticles (NPs) were synthesized through the polyol method, and characterized using spherical aberration (Cs) corrected scanning transmission electron microscopy (STEM). High-angle annular dark-field (HAADF)-STEM images of bimetallic nanoparticles were obtained. The contrast of images shows that nanoparticles have an alloy structure with an average size of 8.2 nm. Together with the characterization of nanoparticles, a systematic molecular dynamics simulations study focused on the structural stability and atomic surface segregation trends in 923-atom PdPt alloyed-bimetallic NPs was carried out.

Morphological Analysis of Bronchial Arteries and Variants with Computed Tomography Angiography

The aim of our study was to determine the prevalence of anatomical variants of bronchial arteries using computed tomographic angiography in a population of northeastern Mexico. An observational, transversal, descriptive, comparative, retrospective study was performed using 139 imaging studies of Mexican patients in which we evaluated the following parameters from the left and right bronchial arteries: artery origin, branching pattern, arterial ostium, vertebral level of origin, diameter, and mediastinal trajectory. The anatomies of the bronchial arteries were similar in both genders, except distribution for vertebral origin level

Relación del Malestar Emocional y el Consumo de Alcohol en Adolescentes

RESUMEN Objetivo: determinar la relación y el efecto del malestar emocional en el consumo de alcohol de los adolescentes. Método: estudio cuantitativo transversal, descriptivo y predictivo, llevado a cabo en 200 adolescentes de ambos sexos de una institución pública de educación secundaria de Ciudad del Carmen Campeche, México; durante el mes enero de 2018. Se utilizó una cedula de datos sociodemográficos y antecedentes del consumo de alcohol y el Cuestionario de Factores de Riesgo y de Protección para el Consumo de Drogas. Los datos se procesaron en el programa SPSS a través de la estadística descriptiva e inferencial. Resultados: 19.5% de los adolescentes manifestaron haber ingerido alguna bebida alcohol en algún momento de su vida y el 14% en el último año. 64% presenta malestar emocional, de los cuales destaca la tristeza, alegría, aburrimiento y pensamientos repetitivos. Se observó una relación positiva (rs=.473, p=.003) y efecto (p=.001, f=10.787, gl=1) del malestar emocional con el consumo de alcohol. Conclusión: se pudo identificar que el malestar emocional está asociado a la ingesta de alcohol, esto como una forma de mitigar ese conjunto de emociones y sentimientos negativas y/o positivas característicos de esta etapa. ABSTRACT Objective: determine the relationship and effect of emotional distress on adolescents’ alcohol consumption. Method: quantitative, cross-sectional, descriptive and predictive study was conducted in 200 middle school adolescents of both sexes in Ciudad del Carmen, Campeche, Mexico; during the month of January 2018. A sociodemographic data and alcohol consumption history and the Questionnaire on Risk Factors and Protection for Drug Use was used. Results: 19.5% of the adolescents claimed to have ingested an alcoholic beverage at some time in their life and 14% in the last year. 64% present emotional discomfort, of which sadness, happiness, boredom and repetitive thoughts stand out. A positive relationship was observed (rs=.473, p=.003) and effect (p=.001, f=10.787, gl=1) of emotional distress with alcohol consumption. Conclusion: it was possible to identify that the emotional distress is associated with alcohol consumption, this as a way to mitigate that set of negative and/or positive emotions and feelings characteristics of this stage

Memoria del II Coloquio de verano de investigación de la Escuela de Negocios de ITESO, 2023

La memoria recoge cinco de las ponencias presentadas en el Coloquio de investigación de verano de la Escuela de Negocios, 2023. Durante las presentaciones y el diálogo quisimos hacer énfasis en dos aspectos de nuestra labor universitaria: 1) el fortalecimiento de la Escuela de Negocios como instancia interdepartamental que comparte una misión común; 2) el modo como las tres funciones sustantivas (docencia, investigación y vinculación) se retroalimentan y sostienen mutuamente. Se presentan resúmenes extendidos de los siguientes trabajos: Diagnóstico de cultura organizacional por alumnos del PAP de Gestión del cambio, del talento humano y la efectividad organizacional; Modelo estratégico de sostenibilidad basado en el modelo de flujos descontados (DCF); Laboratorios móviles: impulsando la industria creativa-cultural en Jalisco; Nueva estrategia de comunicación como proceso formativo para empresarios y emprendedores; Economía Social y Solidaria como un elemento para el desarrollo de talleres del sector artesanal.ITESO, A.C

Thymidylate synthase gene variants as predictors of clinical response and toxicity to fluoropyrimidine-based chemotherapy for colorectal cancer

Abstract Background: Fluoropyrimidines form the chemotherapy backbone of advanced and metastatic colorectal cancer (CRC). These drugs are frequently associated with toxicity events that result in dose adjustments and even suspension of the treatment. The thymidylate synthase (TYMS) gene is a potential marker of response and toxicity to fluoropyirimidines as this enzyme is the molecular target of these drugs. Our aim was to assess the association between variants of TYMS with response and toxicity to fluoropyrimidines in patients with CRC in independent retrospective and prospective studies. Methods: Variants namely rs45445694, rs183205964, rs2853542 and rs151264360 of TYMS were genotyped in 105 CRC patients and were evaluated to define their association with clinical response and toxicity to fluoropyrimidines. Additionally, the relationship between genotypes and tumor gene expression was analyzed by quantitative polymerase chain reaction. Results: The 2R/2R (rs45445694) was associated with clinical response (p = 0.05, odds ratio (OR) = 3.45) and severe toxicity (p = 0.0014, OR = 5.21, from pooled data). Expression analysis in tumor tissues suggested a correlation between the 2R/2R genotype and low TYMS expression. Conclusions: The allele 2R (rs45445694) predicts severe toxicity and objective response in advanced CRC patients. In addition, the alleles G(rs2853542) and 6bp-(rs151264360) are independent predictors of response failure to chemotherapy. This is the first study made on a Latin American population that points out TYMS gene variants have predictive values for response and toxicity in patients with CRC treated with fluoropyrimidine-based chemotherapy

Identification of novel synthetic lethal vulnerability in non small cell lung cancer by co targeting TMPRSS4 and DDR1

Finding novel targets in non-small cell lung cancer (NSCLC) is highly needed and identification of synthetic lethality between two genes is a new approach to target NSCLC. We previously found that TMPRSS4 promotes NSCLC growth and constitutes a prognostic biomarker. Here, through large-scale analyses across 5 public databases we identified consistent co-expression between TMPRSS4 and DDR1. Similar to TMPRSS4, DDR1 promoter was hypomethylated in NSCLC in 3 independent cohorts and hypomethylation was an independent prognostic factor of disease-free survival. Treatment with 5-azacitidine increased DDR1 levels in cell lines, suggesting an epigenetic regulation. Cells lacking TMPRSS4 were highly sensitive to the cytotoxic effect of the DDR1 inhibitor dasatinib. TMPRSS4/DDR1 double knock-down (KD) cells, but not single KD cells suffered a G0/G1 cell cycle arrest with loss of E2F1 and cyclins A and B, increased p21 levels and a larger number of cells in apoptosis. Moreover, double KD cells were highly sensitized to cisplatin, which caused massive apoptosis (~40%). In vivo studies demonstrated tumor regression in double KD-injected mice. In conclusion, we have identified a novel vulnerability in NSCLC resulting from a synthetic lethal interaction between DDR1 and TMPRSS4