Chemical composition and in vitro gas production of some legume browse species in subtropical areas of Mexico

The objective of the present study was to determine the chemical composition and in vitro gas production of different legume and wild arboreal pods. Seven seeds of legume browse species, Mexican calabash (Crescentia alata), esculent leadtree (Leucaena esculenta), guamuchil (Phitecellobium dulce), bastard cedar (Guazuma ulmifolia), needle bush (Acacia farnesiana), mimosa (Mimosa sp.) and elephant ear tree (Enterolobium cyclocarpum). Were evaluated for their chemical composition (g/kg DM) and in vitro gas production pattern. Crude Protein was higher for L. esculenta (220) and lower for G. ulmifolia (70). Neutral and acid detergent fiber were higher for G. ulmifolia (687 and 554) and lower for A. farnesiana (267 and 176). Lignin was higher for Mimosa sp. (219) and lower for P. dulce (81). Total gas production (ml gas/g DM) of P. dulce (187) and E. cyclocarpum (164) were higher (P<0.001) than Mimosa sp. The lowest values were for C. alata (108), G. ulmifolia (102), L. esculenta (99) and A. farnesiana (90). The nutritional characteristics of L. esculenta and A. farnesiana might be used as supplements in ruminant diets, due to their major content in CP and in vitro digestibility, representing an alternative protein supplement during dry season

Synthesis and Effect of CaTiO3 formation in CaO·Al2O3 by Solid-State Reaction from CaCO3· Al2O3 and Ti

CaO⋅Al2O3/CaTiO3 material was synthesized by a solid-state reaction. The effects of CaTiO3 formed into the CaO⋅Al2O3 during the synthesized process on the microstructure and mechanical properties were studied. CaCO3 was obtained from snail shells, Al2O3, and Ti powders were employed as a raw material. A chemical system with a 1:1 M ratio between CaCO3 and Al2O3 was formed, with an addition of 10 wt% Ti metallic particles. The microstructural analysis through optical microscopy, scanning electron microscopy, and X-ray diffraction was realized. The apparent density was determined by the Archimedes method, and the mechanical behavior (hardness, fracture toughness, and transversal elastic modulus) were also determined. XRD analysis revealed the formation of calcium aluminate phases such as CaAl2O4, CaAl4O7, and CaAl12O19; in addition, the CaTiO3 phase was also identified. The in-situ formation of CaTiO3 in the ceramic material, improves the mechanical properties such as the transversal elastic modulus and fracture toughness (48 GPa and 1.32 MPa m1/2, respectively) in comparison to the reference material (55 GPa and 0.43 MPa m1/2, respectively). Changes in the microstructural morphology, bulk density, and hardness values were also observed with a positive effect in the compound material

Virus orf en humanos, confirmación molecular de un caso clínico en Chile.

La infección por el virus orf, también conocida como ectima contagioso, es reconocida una zoonosis ocupacional. Se diagnostica por lesiones cutáneas que evolu cionan rápidamente desde máculas a pápulas, vesículas y pústulas. Se presenta el caso clínico de una estudiante de medicina veterinaria que había tenido contacto con caprinos, clínicamente sanos y sin lesiones aparentes, hacía 19 días. Presentó dos lesiones vesiculares que coalescieron hasta formar una lesión de mayor tamaño rodeada por un halo eritematoso. Las lesiones fueron compatibles con la presentación clásica de las producidas por el virus orf en humanos. Se confirmó la presencia del virus orf mediante una RPC anidada del tejido de biopsia. Es uno de los primeros casos confirmados mediante técnicas moleculares en seres humanos en Chile.Infection with the orf virus, also known as contagious ecthyma, is recognized as an occupational zoonosis worldwide. It is diagnosed by cutaneous lesions that progress rapidly from macules to papules, vesicles and pustules. The clinical case of a student of veterinary medicine who had had contact with goats, clinically healthy and without apparent lesions, which occured 19 days ago, is reported. She presented two vesicular lesions that coalesced to form a larger lesion surrounded by an erythematous halo. The lesions were compatible with the classical presentation of those produced by the orf virus in humans. The presence of the orf virus was confirmed by a nested PCR from biopsy tissue. It is one of the first cases confirmed by molecular techniques in humans in Chile.EEA BalcarceFil: Flores, Carlos. Universidad Mayor. Facultad de Ciencia; Chile.Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Madariaga, Carolina. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria; Chile.Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina

Characterization of the resistance of tomato accessions from the bgh-ufv to the geminivírus tomato yellow spot virus

The viruses transmitted by whiteflies are among those causing relevant losses in tomato cultivation. Among the measures to control these agents, introducing genes for resistance constitutes the main control measure, together with vector control. The objective of this work was to screen for sources of natural resistance to Tomato yellow spot virus (ToYSV) in S. lycopersicum germplasm from the Banco de Germoplasma de Hortaliças (BGH) of the Universidade Federal de Viçosa (UFV), Minas Gerais, Brazil. The 99 accessions and two susceptible controls were inoculated using biolistics. Inoculated plants were grown under greenhouse conditions. The percentage of plants displaying virus symptoms was evaluated at 10, 20 and 30 days after inoculation (DAI). Virus presence or absence in the inoculated plants was confirmed by hybridization with probes labeled with á-[32P]-dCTP, for each evaluation date. Inoculated plants produced typical disease symptoms showing different behavior on the genotypes in relation to ToYSV. Some of the evaluated genotypes showed higher virus tolerance compared to two susceptible controls, in particular the accessions BGH-2039V and BGH-2041 which showed no symptoms and no viral DNA accumulation in 80% of the inoculated plants at 30 DAI. The results suggest that the selected tomato accesses are good sources of resistance to new tomato cultivars tolerant to ToYSV.Os vírus transmitidos por moscas brancas estão entre aqueles que causam perdas relevantes na cultura do tomate. Entre as medidas para o controle desses agentes, a introdução de genes de resistência constitui a principal medida de controle, juntamente com o controle de vetores. O objetivo deste trabalho foi procurar fontes de resistência natural ao Tomato yellow spot virus (ToYSV) em germoplasma de S. lycopersicum do Banco de Germoplasma de Hortaliças (BGH) da Universidade Federal de Viçosa (UFV), Minas Gerais, Brasil. Noventa e nove subamostras e dois controles suscetíveis foram inoculados usando biobalística. As plantas inoculadas foram mantidas em casa de vegetação. A porcentagem de plantas exibindo sintomas do vírus foi avaliada aos 10, 20 e 30 dias após a inoculação (DAI). A presença ou ausência do vírus nas plantas inoculadas foi confirmada por hibridização com sondas marcadas com á-[32P]-dCTP, para cada data de avaliação. Plantas inoculadas produziram sintomas típicos da doença mostrando diferentes comportamentos dos genótipos em relação ao ToYSV. Alguns dos genótipos avaliados apresentaram maior tolerância ao vírus em relação aos dois controles suscetíveis, em particular as subamostras BGH-2039V e BGH-2041, que não apresentaram sintomas e no tinham acúmulo de DNA viral em 80% das plantas inoculadas aos 30 DAI. Os resultados sugerem que as subamostras de tomate selecionadas são boas fontes de resistência para novas cultivares de tomateiro tolerantes ao ToYSV

Prognostic significance of pathological response after neoadjuvant chemotherapy or chemoradiation for locally advanced cervical carcinoma

BACKGROUND: Cisplatin-based chemoradiation is the standard of care for locally advanced cervical cancer patients; however, neoadjuvant modalities are currently being tested. Neoadjuvant studies in several tumor types have underscored the prognostic significance of pathological response for survival; however there is a paucity of studies in cervical cancer investigating this issue. METHODS: Four cohorts of patients with locally advanced cervical carcinoma (stages IB2-IIIB); included prospectively in phase II protocols of either neoadjuvant chemotherapy with 1) cisplatin-gemcitabine, 2) oxaliplatin-gemcitabine, 3) carboplatin-paclitaxel or 4) chemoradiation with cisplatin or cisplatin-gemcitabine followed by radical hysterectomy were analyzed for pathological response and survival. RESULTS: One-hundred and fifty three (86%) of the 178 patients treated within these trials, underwent radical hysterectomy and were analyzed. Overall, the mean age was 44.7 and almost two-thirds were FIGO stage IIB. Pathological response rates were as follows: Complete (pCR) in 60 cases (39.2%), Near-complete (p-Near-CR) in 24 (15.6 %) and partial (pPR) in 69 cases (45.1%). A higher proportion rate of pCR was observed in patients treated with chemoradiotherapy (with cisplatin [19/40, 47.5%]; or with cisplatin-gemcitabine [24/41, 58.5%] compared with patients receiving only chemotherapy, 6/23 (26%), 3/8 (37.5%) and 8/41 (19.5%) for cisplatin-gemcitabine, oxaliplatin-gemcitabine and carboplatin-paclitaxel respectively [p = 0.0001]). A total of 29 relapses (18.9%) were documented. The pathological response was the only factor influencing on relapse, since only 4/60 (6.6%) patients with pCR relapsed, compared with 25/93 (26.8%) patients with viable tumor, either pNear-CR or pPR (p = 0.001). Overall survival was 98.3% in patients with pCR versus 83% for patients with either pNear-CR or pPR (p = 0.009). CONCLUSION: Complete pathological response but no Near-complete and partial responses is associated with longer survival in cervical cancer patients treated with neoadjuvant chemotherapy or chemoradiotherapy

Whole-Blood Mitochondrial DNA Copies Are Associated With the Prognosis of Acute Respiratory Distress Syndrome After Sepsis.

https://pubmed.ncbi.nlm.nih.gov/34557198/#:~:text=Resumen-,El%20s%C3%ADndrome%20de%20dificultad%20respiratoria%20aguda%20(SDRA)%20es%20un%20proceso,v%C3%ADnculos%20mec%C3%A1nicos%20de%20esta%20observaci%C3%B3n%20con%20la%20patogenia%20del%20SDRA.,-Palabras%20clave%3A%20SDR

Impact of SARS-Cov-2 infection in patients with hypertrophic cardiomyopathy : results of an international multicentre registry

To describe the natural history of SARS-CoV-2 infection in patients with hypertrophic cardiomyopathy (HCM) compared with a control group and to identify predictors of adverse events. Three hundred and five patients [age 56.6 ± 16.9 years old, 191 (62.6%) male patients] with HCM and SARS-Cov-2 infection were enrolled. The control group consisted of 91 131 infected individuals. Endpoints were (i) SARS-CoV-2 related mortality and (ii) severe clinical course [death or intensive care unit (ICU) admission]. New onset of atrial fibrillation, ventricular arrhythmias, shock, stroke, and cardiac arrest were also recorded. Sixty-nine (22.9%) HCM patients were hospitalized for non-ICU level care, and 21 (7.0%) required ICU care. Seventeen (5.6%) died: eight (2.6%) of respiratory failure, four (1.3%) of heart failure, two (0.7%) suddenly, and three (1.0%) due to other SARS-CoV-2-related complications. Covariates associated with mortality in the multivariable were age {odds ratio (OR) per 10 year increase 2.25 [95% confidence interval (CI): 1.12-4.51], P = 0.0229}, baseline New York Heart Association class [OR per one-unit increase 4.01 (95%CI: 1.75-9.20), P = 0.0011], presence of left ventricular outflow tract obstruction [OR 5.59 (95%CI: 1.16-26.92), P = 0.0317], and left ventricular systolic impairment [OR 7.72 (95%CI: 1.20-49.79), P = 0.0316]. Controlling for age and sex and comparing HCM patients with a community-based SARS-CoV-2 cohort, the presence of HCM was associated with a borderline significant increased risk of mortality OR 1.70 (95%CI: 0.98-2.91, P = 0.0600). Over one-fourth of HCM patients infected with SARS-Cov-2 required hospitalization, including 6% in an ICU setting. Age and cardiac features related to HCM, including baseline functional class, left ventricular outflow tract obstruction, and systolic impairment, conveyed increased risk of mortality

Planeación, gobernanza y sustentabilidad Retos y desafíos desde el enfoque territorial

Frente a la compleja realidad actual, resulta ineludible el desarrollo de la investigación científica de los fenómenos y procesos urbanos, territoriales y ambientales, que contribuya a su comprensión y la construcción de alternativas de solución a los retos y desafíos vigentes. En este contexto, el abordaje de las ciudades y regiones metropolitanas, el ordenamiento del territorio y la ocupación del espacio, así como la relación sociedad-naturaleza y la complejidad ambiental, precisa la generación de metodologías y procesos de investigación multi e inter disciplinarios que contribuyan a la comprensión de los procesos socioterritoriales, el mejoramiento de las condiciones de vida y la conservación ambiental.Programa de Fortalecimiento de la Calidad Educativa PFCE-2016 proyecto K0313101

Multicentric study of cervical cancer screening with human papillomavirus testing and assessment of triage methods in Latin America : the ESTAMPA screening study protocol

Q1Q1Introduction Human papillomavirus (HPV) testing is replacing cytology in primary screening. Its limited specificity demands using a second (triage) test to better identify women at high-risk of cervical disease. Cytology represents the immediate triage but its low sensitivity might hamper HPV testing sensitivity, particularly in low-income and middle-income countries (LMICs), where cytology performance has been suboptimal. The ESTAMPA (EStudio multicéntrico de TAMizaje y triaje de cáncer de cuello uterino con pruebas del virus del PApiloma humano; Spanish acronym) study will: (1) evaluate the performance of different triage techniques to detect cervical precancer and (2) inform on how to implement HPV-based screening programmes in LMIC. Methods and analysis Women aged 30–64 years are screened with HPV testing and Pap across 12 study centres in Latin America. Screened positives have colposcopy with biopsy and treatment of lesions. Women with no evident disease are recalled 18 months later for another HPV test; those HPV-positive undergo colposcopy with biopsy and treatment as needed. Biological specimens are collected in different visits for triage testing, which is not used for clinical management. The study outcome is histological high-grade squamous intraepithelial or worse lesions (HSIL+) under the lower anogenital squamous terminology. About 50 000 women will be screened and 500 HSIL+ cases detected (at initial and 18 months screening). Performance measures (sensitivity, specificity and predictive values) of triage techniques to detect HSIL+ will be estimated and compared with adjustment by age and study centre. Ethics and dissemination The study protocol has been approved by the Ethics Committee of the International Agency for Research on Cancer (IARC), of the Pan American Health Organisation (PAHO) and by those in each participating centre. A Data and Safety Monitoring Board (DSMB) has been established to monitor progress of the study, assure participant safety, advice on scientific conduct and analysis and suggest protocol improvements. Study findings will be published in peer-reviewed journals and presented at scientific meetings. Trial registration number NCT01881659Revista Internacional - Indexad

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality