Methodological Analysis about the Potential Avoidabilty of Motor Vehicles Colliding Against Pedestrians in Urban Areas

[EN] The main motivation of the authors of this article is to establish a rigorous definition of the potential capacity that a motor vehicle driver has to avoid a collision against a pedestrian. Henceforth we will call this capacity avoidability. To calculate the avoidability, it is necessary to analyze time, distance and itinerary, initial position of the pedestrian when exposed to the risk, initial speed; theoretical maximum speed developed by the vehicle and road limit speed; the driver’s reaction time and the influence of the environment; and the interrelation of the initial positions of vehicle and pedestrian with respect to the transversal axis of the road. The definition, categorized by variables, of a driver’s ability to avoid run over a pedestrian in an urban area has an evident usefulness: it allows knowing the influence of the initial speed of a vehicle as an isolated variable and the importance of the road limit speed in the ability to prevent an accident.Cabrerizo Sinca, J.; Campos Cacheda, J.; Perez Diez, F. (2016). Methodological Analysis about the Potential Avoidabilty of Motor Vehicles Colliding Against Pedestrians in Urban Areas. En XII Congreso de ingeniería del transporte. 7, 8 y 9 de Junio, Valencia (España). Editorial Universitat Politècnica de València. 1614-1631. https://doi.org/10.4995/CIT2016.2015.3481OCS1614163

Electroactivación de oocitos de pez cebra (Danio rerio)

[ES] El mejor tratamiento de electroactivación (B3) para oocitos de pez cebra supuso un 43% de activación frente al 18% cuando el estímulo es solo el contacto con agua[EN] In zebrafish, initial egg activation by water after being discharged from the ovarian stroma is followed by normal cleavages only in inseminated eggs. When sperm (genetically active or not) is not used as activating agent, reproductive techniques (as either nuclear transplant or intracytoplasmic sperm injection are ineff icient. In this work, three experiments of egg activation by electric stimuli were performed: In the first, six activation treatments were compared (Voltage × Pulses: 2.76 × 1; 2.76 × 2; 2.76 × 3 and 5.40 × 1; 5.40 × 2; 5.40 × 3). The group 5.40 × 3 showed the best results (32% activated). In the second experiment, an electrical treatment of 20 min was carried out. It consisted in a sequence of three equal electrical stimuli every 10 min (of 1 or 3 consecutive direct current square pulses for 20 ¿s each and applied at two voltage levels, 2.76 V or 5.4 V). It was observed that the number of pulses negatively affected the rates of damaged and lysed eggs. Moreover, only the 20 min treatment with the combination of 3 consecutive pulses at 2.76 V showed significant differences with their respective control group (43% vs 18% activated eggs, p < 0.05). In the third experiment, negative effects of egg ageing were observed. In conclusion, the best activation treatment for intact (non manipulated) zebrafish eggs concerns sequence B3. The electro-activation stimulus proposed here would be the only one available once the egg has already been activated by water.Cardona Costa, J.; Perez-Camps, M.; García Ximenez, F. (2011). Electroactivation of zebrafish (danio rerio) eggs. Spanish Journal of Agricultural Research. 9(1):59-65. http://hdl.handle.net/10251/99491S59659

Determinación de los estilos de aprendizaje en los estudiantes de sexto grado de la institución educativa Jorge Eliecer Gaitán, municipio de González, Cesar

Ante las dificultades que tienen los estudiantes para el aprendizaje, sumado a esta situación de pandemia en que cambiaron de manera inesperada todos los procesos escolares, no solo el cambio de la modalidad presencial a la virtualidad o semi-virtualidad, según los casos, la forma de evaluación, la manera de impartir los conocimientos, entre otros eventos que circunscriben el ámbito escolar y que como bien sabemos envolvió a todas las comunidades del planeta sin excepción. Se hace necesario buscar soluciones como docentes con la intención de mejorar el aprendizaje de los estudiantes, por lo que los autores del presente trabajo decidieron explorar los estilos de aprendizaje predominantes en sus estudiantes, para aplicar unos talleres basados en los estilos de aprendizaje predominantes en su población objeto de estudio, evaluar los resultados obtenidos para cotejarlos sus aprendizajes precedentes al estudio. De esta manera visibilizar y establecer los estilos de aprendizaje que brindan mejores resultados en el proceso de aprendizaje de los estudiantes focales de este trabajo.Given the difficulties that students have for learning, added to this pandemic situation in which all school processes changed unexpectedly, not only the change from the face-to-face modality to virtuality or semi-virtuality, depending on the case, the form evaluation, the way of imparting knowledge, among other events that circumscribe the school environment and that, as we well know, involved all the communities of the planet without exception. It is necessary to look for solutions as teachers with the intention of improving student learning, so the authors of this work decided to explore the predominant learning styles in their students, to apply workshops based on the predominant learning styles in their population object of study, evaluate the results obtained to compare their learning prior to the study. In this way, make visible and establish the learning styles that provide the best results in the learning process of the focal students of this wor

Methionine adenosyltransferase S-nitrosylation is regulated by the basic and acidic amino acids surrounding the target thiol

S-Adenosylmethionine serves as the methyl donor for many biological methylation reactions and provides the propylamine group for the synthesis of polyamines. S-Adenosylmethionine is synthesized from methionine and ATP by the enzyme methionine adenosyltransferase. The cellular factors regulating S-adenosylmethionine synthesis have not been well defined. Here we show that in rat hepatocytes S-nitrosoglutathione monoethyl ester, a cell-permeable nitric oxide donor, markedly reduces cellular S-adenosylmethionine content via inactivation of methionine adenosyltransferase by S-nitrosylation. Removal of the nitric oxide donor from the incubation medium leads to the denitrosylation and reactivation of methionine adenosyltransferase and to the rapid recovery of cellular S-adenosylmethionine levels. Nitric oxide inactivates methionine adenosyltransferase via S-nitrosylation of cysteine 121. Replacement of the acidic (aspartate 355) or basic (arginine 357 and arginine 363) amino acids located in the vicinity of cysteine 121 by serine leads to a marked reduction in the ability of nitric oxide to S-nitrosylate and inactivate hepatic methionine adenosyltransferase. These results indicate that protein S-nitrosylation is regulated by the basic and acidic amino acids surrounding the target cysteine

Folding of dimeric methionine adenosyltransferase III: identification of two folding intermediates

Methionine adenosyl transferase (MAT) is an essential enzyme that synthesizes AdoMet. The liver-specific MAT isoform, MAT III, is a homodimer of a 43.7-kDa subunit that organizes in three nonsequential alpha-beta domains. Although MAT III structure has been recently resolved, little is known about its folding mechanism. Equilibrium unfolding and refolding of MAT III, and the monomeric mutant R265H, have been monitored using different physical parameters. Tryptophanyl fluorescence showed a three-state folding mechanism. The first unfolding step was a folding/association process as indicated by its dependence on protein concentration. The monomeric folding intermediate produced was the predominant species between 1.5 and 3 m urea. It had a relatively compact conformation with tryptophan residues and hydrophobic surfaces occluded from the solvent, although its N-terminal region may be very unstructured. The second unfolding step monitored the denaturation of the intermediate. Refolding of the intermediate showed first order kinetics, indicating the presence of a kinetic intermediate within the folding/association transition. Its presence was confirmed by measuring the 1,8-anilinonaphtalene-8-sulfonic acid binding in the presence of tripolyphosphate. We propose that the folding rate-limiting step is the formation of an intermediate, probably a structured monomer with exposed hydrophobic surfaces, that rapidly associates to form dimeric MAT III

Transmigrated canines: review and update

Transmigrated canines are impacted canines that have crossed the midline. Objective: To evaluate the epidemiological and clinical aspects that have to do with transmigrated canines. Study design: Bibliographic review of 209 transmigrated canines obtained from a review of articles from 1971 to the present. Results: Transmigrated canines are most frequent among females and are usually diagnosed within the first two decades of life. They are usually located in the mandible, with transmigration being most frequent in the left mandibular canine. In a large number of cases, the deciduous canine remains in the arcade. Transmigrated canines are most often found impacted, the primary position being mesially inclined, having crossed the midline. There is no pathology that is clearly associated with these canines, although radiolucence compatible with a cyst was found in a great percentage. Conclusions: Given that it is not possible to predict when dental transmigration is going to occur, we must monitor those mandibular canines which present a delay in their eruption, in order to try to carry out an interceptive treatment and avoid possible pathologies

An exploratory study comparing psychological profiles and its congruence with clinical performance among patients with functional or motility digestive disorders

Functional gastrointestinal disorders have been related with different psychological conditions. On the contrary, the role of psychological factors within gastrointestinal motor disorders remains unclear. The objective of this study was to explore the differences and congruence with clinical performance of the psychological profile and subjective functionality among patients diagnosed with functional gastrointestinal disorders and gastrointestinal motor disorders. Using a double-blind design, 56 inpatients from a Gastroenterology Department were included in the study. No major differences were detected between the two groups. However, clinical performance was coherent with subjective physical functioning only among patients diagnosed with gastrointestinal motor disorders. These results may provide useful information for gastroenterologists dealing with patients' complaints not consistent with their clinical profile

Implementation of Processing Functions for Autonomous Power Quality Measurement Equipment: A Performance Evaluation of CPU and FPGA-Based Embedded System

Motivated by the effects of deregulation over power quality and the subsequent need of new types of measurements, this paper assesses different implementations of an estimate for the spectral kurtosis, considered as a low-level harmonic detection. Performance of a processor-based system is compared with a field programmable gate array (FPGA)-based solution, in order to evaluate the accuracy of this processing function for implementation in autonomous measurement equipment. The fourth-order spectrum, with applications in different fields, needs advanced digital signal processing, making it necessary to compare implementation alternatives. In order to obtain reproducible results, the implementations have been developed using common design and programming tools. Several characteristics of the implementations are compared, showing that the increasing complexity and reduced cost of the current FPGA models make the implementation of complex mathematical functions feasible. We show that FPGAs improve the processing capability of the best processor using an operating frequency 33 times lower. This fact strongly supports its implementation in hand-held instruments