1,973 research outputs found
The root-soil system of Norway spruce subjected to turning moment: resistance as a function of rotation
The reactions of trees to wind, rockfall, and snow and debris flow depend largely on how strong and deformable their anchorage in the soil is. Here, the resistive turning moment M of the root–soil system as a function of the rotation ϕ at the stem base plays the major role. M(ϕ) describes the behavior of the root– soil system when subject to rotational moment, with the maximum M(ϕ) indicating the anchorage strength M a of the tree. We assessed M(ϕ) of 66 Norway spruce (Picea abies L. Karst) by pulling them over with a winch. These 45- to 170-year-old trees grew at sites of low and high elevation, with a diameter at breast height DBH = 14–69 cm and a height H = 9–42 m. M(ϕ) displayed a strong nonlinear behavior. M a was reached at a lower ϕ for large trees than for small trees. Thus overhanging tree weight contributed less to M a for the large trees. Overturning also occurred at a lower ϕ for the large trees. These observations show that the rotational ductility of the root–soil system is higher for small trees. M a could be described by four monovariate linear regression equations of tree weight, stem weight, stem volume and DBH ² ·H (0.80 R ² ϕ at M a, ϕ a, by a power law of DBH²·H (R ² = 0.85). We found significantly higher M a for the low-elevation spruces than for the high-elevation spruces, which were more shallowly anchored, but no significant difference in ϕ a. The 66 curves of M(ϕ), normalized (n) by M a in M-direction and by ϕ a in ϕ-direction, yielded one characteristic average curve: Mn (ϕn) M¯nϕn . Using this average curve and the predictions of M a and ϕ a, it is shown that M(ϕ) and the curves associated with M(ϕ) can be predicted with a relative standard error ≤25%. The parameterization of M(ϕ) by tree size and weight is novel and provides useful information for predicting with finite-element computer models how trees will react to natural hazards
Force generation in small ensembles of Brownian motors
The motility of certain gram-negative bacteria is mediated by retraction of
type IV pili surface filaments, which are essential for infectivity. The
retraction is powered by a strong molecular motor protein, PilT, producing very
high forces that can exceed 150 pN. The molecular details of the motor
mechanism are still largely unknown, while other features have been identified,
such as the ring-shaped protein structure of the PilT motor. The surprisingly
high forces generated by the PilT system motivate a model investigation of the
generation of large forces in molecular motors. We propose a simple model,
involving a small ensemble of motor subunits interacting through the
deformations on a circular backbone with finite stiffness. The model describes
the motor subunits in terms of diffusing particles in an asymmetric,
time-dependent binding potential (flashing ratchet potential), roughly
corresponding to the ATP hydrolysis cycle. We compute force-velocity relations
in a subset of the parameter space and explore how the maximum force (stall
force) is determined by stiffness, binding strength, ensemble size, and degree
of asymmetry. We identify two qualitatively different regimes of operation
depending on the relation between ensemble size and asymmetry. In the
transition between these two regimes, the stall force depends nonlinearly on
the number of motor subunits. Compared to its constituents without
interactions, we find higher efficiency and qualitatively different
force-velocity relations. The model captures several of the qualitative
features obtained in experiments on pilus retraction forces, such as roughly
constant velocity at low applied forces and insensitivity in the stall force to
changes in the ATP concentration.Comment: RevTex 9 pages, 4 figures. Revised version, new subsections in Sec.
III, removed typo
The distribution of a germline methylation marker suggests a regional mechanism of LINE-1 silencing by the piRNA-PIWI system
<p>Abstract</p> <p>Background</p> <p>A defense system against transposon activity in the human germline based on PIWI proteins and piRNA has recently been discovered. It represses the activity of LINE-1 elements via DNA methylation by a largely unknown mechanism. Based on the dispersed distribution of clusters of piRNA genes in a strand-specific manner on all human chromosomes, we hypothesized that this system might work preferentially on local and proximal sequences. We tested this hypothesis with a methylation-associated SNP (mSNP) marker which is based on the density of C-T transitions in CpG dinucleotides as a surrogate marker for germline methylation.</p> <p>Results</p> <p>We found significantly higher density of mSNPs flanking piRNA clusters in the human genome for flank sizes of 1-16 Mb. A dose-response relationship between number of piRNA genes and mSNP density was found for up to 16 Mb of flanking sequences. The chromosomal density of hypermethylated LINE-1 elements had a significant positive correlation with the chromosomal density of piRNA genes (<it>r </it>= 0.41, <it>P </it>= 0.05<it>)</it>. Genome windows of 1-16 Mb containing piRNA clusters had significantly more hypermethylated LINE-1 elements than windows not containing piRNA clusters. Finally, the minimum distance to the next piRNA cluster was significantly shorter for hypermethylated LINE-1 compared to normally methylated elements (14.4 Mb vs 16.1 Mb).</p> <p>Conclusions</p> <p>Our observations support our hypothesis that the piRNA-PIWI system preferentially methylates sequences in close proximity to the piRNA clusters and perhaps physically adjacent sequences on other chromosomes. Furthermore they suggest that this proximity effect extends up to 16 Mb. This could be due to an unknown localization signal, transcription of piRNA genes near the nuclear membrane or the presence of an unknown RNA molecule that spreads across the chromosome and targets the methylation directed by the piRNA-PIWI complex. Our data suggest a region specific molecular mechanism which can be sought experimentally.</p
Video-Rate Switching of High-Reflectivity Hybrid Cavities Spanning All Primary Colors
Dynamically tunable reflective structural colors are attractive for reflective displays (electronic paper). However, it is challenging to tune a thin layer of structural color across the full red–green–blue (RGB) basis set of colors at video rates and with long-term stability. In this work, this is achieved through a hybrid cavity built from metal–insulator–metal (MIM) “nanocaves” and an electrochromic polymer (PProDOTMe2). The reflective colors are modulated by electrochemically doping/dedoping the polymer. Compared with traditional subpixel-based systems, this hybrid structure provides high reflectivity (>40%) due to its “monopixel” nature and switches at video rates. The polymer bistability helps deliver ultralow power consumption (≈2.5\ua0mW\ua0cm−2) for video display applications and negligible consumption (≈3\ua0\ub5W\ua0cm−2) for static images, compatible with fully photovoltaic powering. In addition, the color uniformity of the hybrid material is excellent (over cm−2) and the scalable fabrication enables large-area production
The Qualified Legal Compliance Committee: Using the Attorney Conduct Rules to Restructure the Board of Directors
The Securities and Exchange Commission introduced a new corporate governance structure, the qualified legal compliance committee, as part of the professional standards of conduct for attorneys mandated by the Sarbanes-Oxley Act of 2002. QLCCs are consistent with the Commission\u27s general approach to improving corporate governance through specialized committees of independent directors. This Article suggests, however, that assessing the benefits and costs of creating QLCCs may be more complex than is initially apparent. Importantly, QLCCs are unlikely to be effective in the absence of incentives for active director monitoring. This Article concludes by considering three ways of increasing these incentives
The STAR MAPS-based PiXeL detector
The PiXeL detector (PXL) for the Heavy Flavor Tracker (HFT) of the STAR
experiment at RHIC is the first application of the state-of-the-art thin
Monolithic Active Pixel Sensors (MAPS) technology in a collider environment.
Custom built pixel sensors, their readout electronics and the detector
mechanical structure are described in detail. Selected detector design aspects
and production steps are presented. The detector operations during the three
years of data taking (2014-2016) and the overall performance exceeding the
design specifications are discussed in the conclusive sections of this paper
Backward Reachability of Array-based Systems by SMT solving: Termination and Invariant Synthesis
The safety of infinite state systems can be checked by a backward
reachability procedure. For certain classes of systems, it is possible to prove
the termination of the procedure and hence conclude the decidability of the
safety problem. Although backward reachability is property-directed, it can
unnecessarily explore (large) portions of the state space of a system which are
not required to verify the safety property under consideration. To avoid this,
invariants can be used to dramatically prune the search space. Indeed, the
problem is to guess such appropriate invariants. In this paper, we present a
fully declarative and symbolic approach to the mechanization of backward
reachability of infinite state systems manipulating arrays by Satisfiability
Modulo Theories solving. Theories are used to specify the topology and the data
manipulated by the system. We identify sufficient conditions on the theories to
ensure the termination of backward reachability and we show the completeness of
a method for invariant synthesis (obtained as the dual of backward
reachability), again, under suitable hypotheses on the theories. We also
present a pragmatic approach to interleave invariant synthesis and backward
reachability so that a fix-point for the set of backward reachable states is
more easily obtained. Finally, we discuss heuristics that allow us to derive an
implementation of the techniques in the model checker MCMT, showing remarkable
speed-ups on a significant set of safety problems extracted from a variety of
sources.Comment: Accepted for publication in Logical Methods in Computer Scienc
Spiral-Induced Star Formation in the Outer Disks of Galaxies
The outer regions of galactic disks have received increased attention since
ultraviolet observations with GALEX demonstrated that nearly 30% of galaxies
have UV emission beyond their optical extents, indicating star formation
activity. These galaxies have been termed extended UV (XUV) disks. Here, we
address whether these observations contradict the gas surface density threshold
for star formation inferred from Halpha radial profiles of galaxies. We run
smoothed particle hydrodynamics simulations of isolated disk galaxies with
fiducial star formation prescriptions and show that over-densities owing to the
presence of spiral structure can induce star formation in extended gas disks.
For direct comparison with observations, we use the 3-D radiative transfer code
Sunrise to create simulated FUV and K_s band images. We find that galaxies
classified as Type I XUV disks are a natural consequence of spiral patterns,
but we are unable to reproduce Type II XUV disks. We also compare our results
to studies of the Kennicutt-Schmidt relation in outer disks.Comment: Published in Ap
Porcine endogenous retroviruses PERV A and A/C recombinant are insensitive to a range of divergent mammalian TRIM5 proteins including human TRIM5
The potential risk of cross-species transmission of porcine endogenous retroviruses (PERV) to humans has slowed the development of xenotransplantation, using pigs as organ donors. Here, we show that PERVs are insensitive to restriction by divergent TRIM5{alpha} molecules despite the fact that they strongly restrict a variety of divergent lentiviruses. We also show that the human PERV A/C recombinant clone 14/220 reverse transcribes with increased efficiency in human cells, leading to significantly higher infectivity. We conclude that xenotransplantation studies should consider the danger of highly infectious TRIM5{alpha}-insensitive human-tropic PERV recombinants
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