Key principles for effective marine governance, including lessons learned after decades of adaptive management in the Great Barrier Reef

This paper reviews the concept of governance in protected areas, providing details about nine key principles of governance as they relate to marine protected areas (MPAs). Following a theoretical description of each principle, real-world examples of the principles are presented from the Great Barrier Reef (GBR) Marine Park, where marine governance has evolved over 45 years as part of adaptive management. Examples of good governance in the GBR include the intergovernmental arrangements that enable both federal and state governments to co-operate effectively across adjoining marine jurisdictions. In addition, the application of multiple layers of management adds to an effective integrated approach, considered to be the most appropriate for managing a large MPA. The nine governance principles discussed in the paper are applicable to all MPAs, but how they are applied will vary in dissimilar settings because of differing environmental, social, economic, cultural, and political contexts - clearly, one size does not fit all. The analogy of the nine principles being part of an interlaced or woven ‘lattice’ is also introduced. Collectively the lattice is stronger than any individual principle, and together all principles contribute to the totality of effective governance. The paper provides information for those involved in MPA management who are keen to understand marine governance and how it might apply to their MPA, recognising there will be differences in how the principles will apply

Assessing the climate vulnerability of the world’s natural and cultural heritage

Climate change is the fastest-growing global threat to the world’s natural and cultural heritage. No systematic approach to assess climate vulnerability of protected areas and their associated communities has existed—until now. The Climate Vulnerability Index (CVI) is scientifically robust, transparent, and repeatable, and has now been applied to various World Heritage properties. The CVI builds upon an established Intergovernmental Panel on Climate Change (IPCC) framework to systematically assess vulnerability through a risk assessment approach that considers the key values of the World Heritage property in question and identifies key climate stressors. The CVI process is then used to assess the climate-related vulnerability of the community (including local residents, domestic visitors, and international tourists) associated with the World Heritage property considering economic, social, and cultural connections. Climate impacts are increasingly adding to a wide range of compounding pressures (e.g., increasing tourism, infrastructure development, changing land use practices) that are affecting places, people, customs, and values. Applications of the CVI to date have led to commitments to integrate outcomes into relevant management plans, and to periodically repeat the process, enabling responsive management to changing future circumstances. The CVI has also demonstrated its potential applicability for protected areas beyond World Heritage properties. The CVI process engages local community members in determining impacts, provides opportunities for identifying adaptation and impact mitigation within the community, and aids broader communication about key climate issues

Ensuring effective and transformative policy reform: lessons from rezoning Australia's Great Barrier Reef, 1999-2004

Jon Day studied the process to develop Great Barrier Reef Marine Park Zoning Plan as an example of transformative environmental policy reform at an ecosystem-wide scale. This was a comprehensive assessment of policy reform, investigating the key factors that influenced the successful policy outcome. Many lessons will help develop environmental policies elsewhere

Biodiversity of the Great Barrier Reef: how adequately is it protected?

Background: The Great Barrier Reef (GBR) is the world's most iconic coral reef ecosystem, recognised internationally as a World Heritage Area of outstanding significance. Safeguarding the biodiversity of this universally important reef is a core legislative objective; however, ongoing cumulative impacts including widespread coral bleaching and other detrimental impacts have heightened conservation concerns for the future of the GBR. Methods: Here we review the literature to report on processes threatening species on the GBR, the status of marine biodiversity, and evaluate the extent of species-level monitoring and reporting. We assess how many species are listed as threatened at a global scale and explore whether these same species are protected under national threatened species legislation. We conclude this review by providing future directions for protecting potentially endangered elements of biodiversity within the GBR. Results: Most of the threats identified to be harming the diversity of marine life on the GBR over the last two-three decades remain to be effectively addressed and many are worsening. The inherent resilience of this globally significant coral reef ecosystem has been seriously compromised and various elements of the biological diversity for which it is renowned may be at risk of silent extinction. We show at least 136 of the 12,000+ animal species known to occur on the GBR (approximately 20% of the 700 species assessed by the IUCN) occur in elevated categories of threat (Critically Endangered, Endangered or Vulnerable) at a global scale. Despite the wider background level of threat for these 136 species, only 23 of them are listed as threatened under regional or national legislation. Discussion: To adequately protect the biodiversity values of the GBR, it may be necessary to conduct further targeted species-level monitoring and reporting to complement ecosystem management approaches. Conducting a vigorous value of information analysis would provide the opportunity to evaluate what new and targeted information is necessary to support dynamic management and to safeguard both species and the ecosystem as a whole. Such an analysis would help decision-makers determine if further comprehensive biodiversity surveys are needed, especially for those species recognised to be facing elevated background levels of threat. If further monitoring is undertaken, it will be important to ensure it aligns with and informs the GBRMPA Outlook five-year reporting schedule. The potential also exists to incorporate new environmental DNA technologies into routine monitoring to deliver high-resolution species data and identify indicator species that are cursors of specific disturbances. Unless more targeted action is taken to safeguard biodiversity, we may fail to pass onto future generations many of the values that comprise what is universally regarded as the world's most iconic coral reef ecosystem

SUMOylation of DISC1: a potential role in neural progenitor proliferation in the developing cortex

DISC1 is a multifunctional, intracellular scaffold protein. At the cellular level, DISC1 plays a pivotal role in neural progenitor proliferation, migration, and synaptic maturation. Perturbation of the biological pathways involving DISC1 is known to lead to behavioral changes in rodents, which supports a clinical report of a Scottish pedigree in which the majority of family members with disruption of the DISC1 gene manifest depression, schizophrenia, and related mental conditions. The discrepancy between modest evidence in genetics and strong biological support for the role of DISC1 in mental conditions suggests a working hypothesis that regulation of DISC1 at the protein level, such as posttranslational modification, may play a role in the pathology of mental conditions. In this study, we report on the SUMOylation of DISC1. This posttranslational modification occurs on lysine residues where the small ubiquitin-related modifier (SUMO) and its homologs are conjugated to a large number of cellular proteins, which in turn regulates their subcellular distribution and protein stability. By using in silico, biochemical, and cell-biological approaches, we now demonstrate that human DISC1 is SUMOylated at one specific lysine 643 (K643). We also show that this residue is crucial for proper neural progenitor proliferation in the developing cortex

Bone Mineral Density and Associated Genetic Variants in High-level Caucasian Marathon Runners

INTRODUCTION:Endurance runners (except those who may have low energy availability) tend to have higher total and/or loading site-specific bone mineral density (BMD) in comparison with non-athletes, most likely due to the larger volume of exercise completed. A large genetic component also contributes to BMD, although little is known about which specific genes are involved, whether particular genotypes are sensitive to mechanical loading and the impact of such an interaction on BMD. This study investigated if high-level endurance runners possess enhanced BMD associated with an “advantageous” genetic predisposition, via a potential gene-physical activity interaction.METHODS:Age- and weight-adjusted total BMD (TBMD) and leg BMD (LBMD) measured via Dual-energy X-ray absorptiometry of 67 high-level Caucasian marathon runners (males < 2 h 45 min, n = 37; females < 3 15 min, n = 30) was compared with 40 male and 26 female non-athletes. LRP5 rs3736228, TNFRSF11B rs4355801, VDR rs2228570, WNT16 rs3801387 and AXIN1 rs9921222 variants were then investigated singularly, and collectively, as a total genotype score (TGS) via multivariate analysis of variance in a subgroup of this cohort (male runners n = 19, controls n = 26; female runners n = 17, controls n = 14). RESULTS:Male runners had higher TBMD (1.34 vs 1.28 g/cm2; P=0.02) and LBMD (1.53 vs 1.42 g/cm2; P=<0.01) than non-athletes. Female runners had higher LBMD than non-athletes (1.30 vs 1.22 g/cm2; P=0.02) but not TBMD (1.23 vs 1.18 g/cm2; P=0.22). An interaction (P=0.047) was observed between VDR rs2228570 genotype and group regarding LBMD in males: ff genotype runners had 0.02 g/cm2 higher LBMD than FF or Ff runners, but the FF genotype had the highest LBMD (1.45 g/cm2) amongst non-athletes. LBMD was also 0.12 g/cm2 higher in ff runners compared to ff non-athletes, whereas FF and Ff runners had 0.09 g/cm2 higher LBMD compared to their genotype-matched controls. No other interactions or variants, individually or collectively as part of a TGS, were associated with BMD (P≥0.11). CONCLUSION:High-level female runners possess higher LBMD but not TBMD in comparison with non-athletes whereas male runners possess both higher TBMD and LBMD than non-athletes. Consistent with prior literature, we observed higher BMD in VDR rs2228570 FF genotype in non-athletes, which may be due to increased biological activity associated with the F variant. However, our preliminary data suggest that the ff genotype may be associated with enhanced LBMD in male runners via a gene-environment interaction.Peer reviewedFinal Published versio

Impacts of climate change on World Heritage coral reefs: a first global scientific assessment

Since 1972, the UNESCO World Heritage Convention has united the world around a shared responsibility to protect natural and cultural places of Outstanding Universal Value (OUV). The World Heritage List includes 29 natural, marine properties that contain coral reef systems. Stretching around the planet, these globally significant reefs include icons such as the Phoenix Islands Protected Area (Kiribati), the Great Barrier Reef (Australia), Papahānaumokuākea (USA), Belize Barrier Reef Reserve System (Belize) and Tubbataha Reefs Natural Park (Philippines). They are recognized for their unique and global importance and for being part of the common heritage of humanity. Coral reefs are ecologically and economically important ecosystems found across the world’s tropical and sub-tropical oceans. Despite covering less than 0.1% of the ocean floor, reefs host more than one quarter of all marine fish species (in addition to many other marine animals). They are the most inherently biodiverse ecosystems in the ocean – comparable to rainforests on land. These ‘Rainforests of the Sea’ provide social, economic and cultural services with an estimated value of over USD $1 Trillion globally. For example, the complex three-dimensional structure of reefs not only provides habitat but also dissipates wave energy to protect coastlines from erosion and damage. Coastal protection and human use (including tourism, recreation and fishing) supply the greatest economic benefits from coral reefs to over half a billion people around the world. Despite their importance and value, most coral reefs are under enormous pressure from a range of different human activities globally including agricultural run-off, urban development, and over-fishing. Superimposed on these local threats, increased ocean temperature has caused the death of corals around the world in recent years. At this point, rising atmospheric carbon dioxide caused by human activity is the greatest threat to coral reefs globally, primarily due to ocean warming but also due to ocean acidification that ensues

Synthesis of bis(ethylenedithio)tetrathiafulvalene (BEDT-TTF) derivatives functionalised with two, four or eight hydroxyl groups

Short synthetic routes to a range of BEDT-TTF derivatives functionalised with two, four or eight hydroxyl groups are reported, of interest because of their potential for introducing hydrogen bonding between donor and anion into their radical cation salts. The cycloaddition of 1,3-dithiole-2,4,5-trithione with alkenes to construct 5,6-dihydro-1,3-dithiolo[4,5-b]1,4-dithiin-2-thiones is a key step, with homo- or hetero-coupling procedures and O-deprotection completing the syntheses. The first synthesis of a single diastereomer of tetrakis(hydroxymethyl)BEDT-TTF, the cis, trans product, was achieved by careful choice of O-protecting groups to facilitate separation of homo- and hetero-coupled products. Cyclisation of the trithione with enantiopure 1R,2R,5R,6R-bis(O,O-isopropylidene)hex-3-ene-1,2,5,6-tetrol (from D-mannitol) gave two separable diastereomeric thiones, which can be transformed to enantiomeric BEDT-TTF derivatives with four or eight hydroxyl groups

Chemical informatics uncovers a new role for moexipril as a novel inhibitor of cAMP phosphodiesterase-4 (PDE4)

PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3′5′ adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but their use in the clinic has been hampered by mechanism-associated side effects that limit maximally tolerated doses. In an attempt to initiate the development of better-tolerated PDE4 inhibitors we have surveyed existing approved drugs for PDE4-inhibitory activity. With this objective, we utilised a high-throughput computational approach that identified moexipril, a well tolerated and safe angiotensin-converting enzyme (ACE) inhibitor, as a PDE4 inhibitor. Experimentally we showed that moexipril and two structurally related analogues acted in the micro molar range to inhibit PDE4 activity. Employing a FRET-based biosensor constructed from the nucleotide binding domain of the type 1 exchange protein activated by cAMP, EPAC1, we demonstrated that moexipril markedly potentiated the ability of forskolin to increase intracellular cAMP levels. Finally, we demonstrated that the PDE4 inhibitory effect of moexipril is functionally able to induce phosphorylation of the Hsp20 by cAMP dependent protein kinase A. Our data suggest that moexipril is a bona fide PDE4 inhibitor that may provide the starting point for development of novel PDE4 inhibitors with an improved therapeutic window