Regeneration of plants from somatic embryos of Verticillium dahliae-resistant wild olive genotypes

Regeneration capacity, via somatic embryogenesis, of several wild olive genotypes differing in their response to Verticillium wilt (resistant genotypes Stop Vert, Out Vert, Ac4 and Ac 18 and the susceptible Ac 15) has been evaluated. To induce somatic embryogenesis, methodologies previously used in cultivated (high ratio cytokinin/auxin) or wild olive (low ratio cytokinin/auxin) were used. Obtained results revealed the importance of genotype, explant type, mineral formulation and hormonal balance in the induction process, ca. use of apical buds obtained from micropropagated shoots following the methodology of Mazri et al. (2013) in cultivated olive (4 days in liquid medium MS ½, 30 µM TDZ – 0.54 µM ANA, 8 weeks in basal medium MS ½, followed by subculturing in ECO basal medium supplemented with 0.5 µM 2iP, 0.44 µM BA and 0.25 µM IBA) was adequate to obtain somatic embryos in 2 genotypes, Stop Vert and Ac18, but no embryogenic response was observed in the other three. An analysis of genetic stability on Stop Vert, using SSR and RAPDs markers, was carried out in embryogenic callus, plants regenerated form this callus and micropropagated shoots in comparison with the mother plant. Polymorphism was only observed in the banding pattern generated by RAPDs in one of the 10 callus samples evaluated, resulting in a variation rate of 0.07%. This is the first time in which plants have been regenerated via somatic embryogenesis in wild olive.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Research project: Junta de Andalucía P11-AGR799

Uso de competiciones y sistemas de clasificación como metodología de evaluación de una asignatura

En este artículo describimos nuestra experiencia en el uso de una metodología de enseñanza activa y participativa orientada al aprendizaje de técnicas de Inteligencia Artificial entre los estudiantes de Informática. En concreto describimos una aproximación basada en competiciones para evaluar las prácticas de los alumnos y un conjunto de herramientas desarrollado para el apoyo de esta metodología. Esta experiencia nos muestra que las competiciones como parte del método de evaluación incrementan la motivación de los alumnos y proporcionan un mecanismo para que el alumno demuestre sus habilidades, sus proyectos, comparta sus intereses y pueda compararse con otros. Así mismo, presentamos los resultados obtenidos en las encuestas realizadas a los alumnos sobre el uso de las competiciones, así como un experimento que analiza la correlación entre las notas reales obtenidas por los alumnos en las prácticas y la posición alcanzada en la competición.Peer Reviewe

La Diferenciación como Estrategia Competitiva Una tarea no tan fácil de ejecutar

A principios de la década de los 80, Michael Porter, un profesor de Estrategia de la Harvard Business School que se ha convertido hoy en uno de los principales gurús del mundo del marketing, publicó el libro “Competitive Strategy”, en el que sentaba las bases de la estrategia de marketing moderna. Entre las principales aportaciones de este trabajo, Porter hablaba de la  necesidad que tenían las empresas de diferenciarse. A pesar de que el mercado actual no tiene nada que ver con el de los 80, el maestro hablaba de tres fórmulas que podían emplear las empresas para mejorar su competitividad y ser duraderas en el tiempo que todavía hoy son aplicables: El liderazgo en Costes, la Estrategia de Enfoque y la Estrategia de diferenciación

Somatic embryogenesis in explants of adult wild olive trees

In this investigation, somatic embryogenesis in explants of adult wild olive trees, using the protocol developed by Mazri et al. (2013, Sci. Hort. 159: 88-95), was induced. Four genotypes differring in the level of resistance to the fungal pathogen Verticillium dahliae were used: Ac18, StopVert and OutVert (symptomless resistant genotypes, Jiménez-Fernández et al. 2015, Plant Pathology, in press) and Ac15 (susceptible genotype, Jiménez-Díaz, IAS-CSIC, Córdoba, personal communication). Three types of explants from actively growing cultures were used: first pair of leaves, petioles and shoot apex. Firstly, all explants were cultured on a liquid induction medium with MS mineral elements at 0.5X and 30 μM TDZ-0.5 μM NAA, for 4 days at 80rpm. Afterwards, explants were transferred to basal MS with 0.5X macroelements, for 8 weeks. Finally, calli were cultured on expression ECO medium supplemented with 0.25 μM IBA, 0.5 μM 2ip and 0.44 μM BA, for several subcultures. Cultures were incubated under darkness at 25 degrees. Embryogenic calli were observed on shoot apex (StopVert) or leaf primordia (Ac18) explants.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Protocolos de descubrimiento de servicios: una mirada conceptual

RESUMENEl crecimiento exponencial de los dispositivos y servicios de red hace necesaria la implementación de protocolos que permitan a los clientes, al tener acceso a una red, detectar automáticamente los servicios ofrecidos en ella. Estos protocolos se conocen como “Protocolos de Descubrimiento de Servicios”. En el siguiente documento se analiza el estado del arte de estos protocolos, así como su funcionamiento, además se realiza Un contraste de las características principales de cada uno de ellos y por último se conocerá a mayor profundidad dos de los protocolos de descubrimiento de servicios populares: UPnP y JINI .ABSTRACTThe exponential growth of network devices and services makes necessary to implement protocols that enable customers, access to a network, automatically detect the services offered in it. These protocols are known as Discovery Protocols Services. The following paper discusses the state of the art these protocols, and its operation, also carried out a contrast the main features of each and Finally you will meet two further protocols popular service discovery: UPnP and JINI

Marketing sensorial y su relación con la fidelización del cliente en el restaurante Miraflores, Chiclayo – 2019

El objetivo de la investigación fue determinar la relación entre el marketing sensorial y fidelización del cliente en el restaurante Miraflores. La metodología fue de tipo correlacional con un diseño no experimental, se tuvo como muestra a 152 clientes a quienes se les aplicó una encuesta. Los resultados mostraron que a los clientes no les llama la atención los anuncios publicitarios, la música, y el volumen no está acorde a la comida, pese ha ello los clientes consideran estar contentos con el sabor de la comida, pero una pequeña brecha indica que no tienen intenciones de volver a degustar los platos del restaurante. Con respecto a la fidelización, gran parte de los clientes considera que se atiende en un horario adecuado, pero el restaurante no ofrece servicios diferentes, los trabajadores no transmiten confianza, a pesar de que brindan un trato amable, pero no atienden todas las consultas, y cuando los clientes han tenido una queja o problema ésta no ha sido solucionada de manera rápida, éstos no pueden realizar sugerencias, es decir no hay cómo. Por otro lado, los productos son elaborados con insumos de calidad y se tiene óptimas condiciones de higiene al momento de la preparación, pero el restaurante no superó las expectativas de los clientes, por lo que la experiencia en general fue regular. Se concluye que existe una relación de 0.709 entre la variable marketing sensorial y la variable fidelización, esto indica que la correlación es alta significativa.TesisGestión empresarial y emprendimient

Propuesta de implementación de micropilotes para estabilizar el talud de relleno artificial adyacente al complejo deportivo Manuel Bonilla en el distrito de Miraflores, ciudad de Lima, 2021

En la presente investigación se realizó el análisis de estabilidad de un talud perteneciente a los acantilados de la Costa Verde, en el cual se ha identificado un gran depósito de relleno antropogénico. Para ello, inicialmente se evaluó la condición estática y pseudoestática aplicando el método de equilibrio límite mediante la metodología de Bishop Simplificado y Morgenstern-Price utilizando el software Slide v.6.004. Los resultados obtenidos del análisis demostraron que los valores de factor de seguridad no satisfacen lo mínimo exigido por la Norma CE.020, es decir, la probabilidad de falla y colapso ante la ocurrencia de un evento sísmico severo es elevada. Actualmente, la presencia de estructuras importantes: coliseo Manuel Bonilla, canchas deportivas, veterinaria municipal, estacionamientos y una ciclovía que recorre el borde superior del acantilado, son concurridas para realizar actividades recreativas y no permiten estabilizar el talud modificando su geometría. En consecuencia, el objetivo principal consistió en proponer la implementación de micropilotes para estabilizar el talud sin recortar el límite de propiedad ni demoler las construcciones existentes. Tras diseñar pantallas de micropilotes de 20 cm de diámetro y luego un segundo análisis, los valores de factor de seguridad garantizaron la estabilidad cumpliendo los requerimientos mínimos. Sin embargo, para definir mejor el comportamiento sísmico del talud y proporcionar niveles de amplificación sísmica generados por su estratigrafía, se recomienda realizar una evaluación dinámica del sitio.In the present research was made the stability analysis of a slope belonging to the Costa Verde cliffs in which a large anthropogenic filler deposit has been identified. To this matter, the static and pseudo static condition were initially evaluated applying the limit equilibrium method using the Simplified Bishop and Morgenstern-Price methodology using the Slide v.6.004 software. The results obtained from the analysis showed that the safety factor values do not meet the minimum required by the CE.020 Code, which means, the probability of failure and collapse in the event of a severe seismic event is high. Nowadays, the presence of important structures: Manuel Bonilla coliseum, sports courts, municipal veterinary, parking lots and a bike path that runs along the top edge of the cliff, are used for recreational activities and stabilizing the slope by modifying its geometry is not allowed. Consequently, the main objective was to propose the implementation of micropiles to stabilize the slope without altering the property boundary or demolish existing constructions. After designing 20 cm diameter micropile walls and then a second analysis, the safety factor values ensured stability by meeting minimum requirements. However, to better define the seismic behavior of the slope and provide levels of seismic amplification generated by its stratigraphy, a dynamic site evaluation is recommended

The Age-Redshift Relation For Luminous Red Galaxies Obtained From the Full Spectrum Fitting and Its Cosmological Implications

The relative age of galaxies at different redshifts can be used to infer the Hubble parameter and put constraints on cosmological models. We select 23,883 quiescent luminous red galaxies (LRGs) from the SDSS DR7 and divide them into four sub-samples according to their velocity dispersions and each sub-sample is further divided into 12 redshift bins. The spectra of the LRGs in each redshift and velocity bin are co-added in order to obtain a combined spectrum with relatively high $S/N$. Adopting the GalexEV/SteLib model, we estimate the mean ages of the LRGs from these combined spectra by the full-spectrum fitting method. We check the reliability of the estimated age by using Monte-Carlo simulations and find that the estimates are robust and reliable. Assuming that the LRGs in each sub-sample and each redshift bin were on average formed at the same time, the Hubble parameter at the present time $H_0$ is estimated from the age--redshift relation obtained for each sub-sample, which is compatible with the $H_0$ value measured by other methods. We demonstrate that a systematic bias (up to $\sim 20$) may be introduced to the $H_0$ estimation because of recent star formation in the LRGs due to the later major mergers at z\la 0.4, but this bias may be negligible for those sub-samples with large velocity dispersions. Using the age--redshift relations obtained from the sub-sample with the largest velocity dispersion or the two sub-samples with high velocity dispersions, we find H_0= 65^{+7}_{-3}\kmsmpc or H_0= 74^{+5}_{-4}\kmsmpc by assuming a spatially flat $\Lambda$CDM cosmology. With upcoming surveys, such as the Baryon Oscillation Spectroscopic Survey (BOSS), even larger samples of quiescent massive LRGs may be obtained, and thus the Hubble parameter can be measured with high accuracy through the age--redshift relation.Comment: 13 pages, 7 figures, accepted for publication in AP

Bicriteria food packaging process optimization in double-layered upright and diagonal multihead weighers

[EN] Double-layered multihead weighing machines contain twice the number of hoppers as present in a simple machine with the same number of heads, which enables additional objective optimization possibilities considering the increased number of combinations among hoppers. This research study deals with bicriteria optimization for double-layered upright and diagonal machines using brute force as the optimization criteria. One of the optimization objectives is related to the target weight; the target weight must be at least and as close as possible to the weight to pack. Furthermore, this study also aims to minimize the time for which a certain portion of a product remains in the hopper while waiting to be selected for package formation. This time is known as priority and is measured based on the number of iterations or the number of packages produced by the machine while the hopper waits to be discharged. For these purposes, Different strategies were tested for both machines, which simultaneously optimize the target weight and the priority of the hoppers, showing the reduction of the extraction of the process in addition to reducing the costs of excess product and its reprocessing.We express our gratitude for the support from Universidad Simon Bolivar, Colombia, and Universitat Politecnica de Valencia.Garcia-Jimenez, R.; García-Díaz, JC.; Pulido-Rojano, AD. (2023). Bicriteria food packaging process optimization in double-layered upright and diagonal multihead weighers. Journal of Computational and Applied Mathematics. 428:1-10. https://doi.org/10.1016/j.cam.2023.11516811042

Changes in dip and frictional properties of the basal detachment controlling orogenic wedge propagation and frontal collapse: the External central Betics case

Thin-skinned fold-and-thrust belts (FTBs) have been extensively studied through both field examples and modelling. The overall dynamics of FTBs are, therefore, well understood. One less understood aspect is the combined influence of across-strike changes in the detachment properties and the basement topography on the behaviour of an orogenic wedge. In this paper, we use field data together with reflection seismic interpretation from the External Zones of the Central Betics FTB, S Spain, to identify a significant increase in the wedge basal dip (a basement "threshold") coinciding with the pinch-out of a weak substrate. This induced both changes to the wedge geometry and to the basal friction, which in turn influenced the wedge dynamics. The changing dynamics led to a transient “stagnation” of the FTB propagation, topographic build-up and subsequent collapse of the FTB front. This in turn fed an important Langhian depocenter made up of mass transport deposits. Coevally with the FTB propagation, extension took place both parallel and perpendicular to the orogenic trend. This case study illustrates how across-strike changes in wedge basal properties can control the detailed behaviour of a developing FTB front, but questions remain regarding the time-space interaction and relative importance of the basal parameters