19 research outputs found
Prebiotic effects of olive pomace powders in the gut: In vitro evaluation of the inhibition of adhesion of pathogens, prebiotic and antioxidant effects
Supplementary data related to this article can be found at https:// doi.org/10.1016/j.foodhyd.2020.106312Olive pomace is a biowaste rich in polyphenols and insoluble dietary fibre with high potential to develop new value chains towards a sustainable and circular bioeconomy. Regarding gut health, olive pomace phenolics and insoluble dietary fibre (after possible fermentation) could act as antioxidants, antimicrobial and prebiotic agents. These potential beneficial effects on the gut were analysed for two powders from olive pomace: liquid-enriched powder (LOPP) - mostly source of phenolics - and pulp-enriched powder (POPP) - main source of insoluble dietary fibre. LOPP and POPP were subjected to an in vitro simulated gastrointestinal digestion followed by in vitro faecal fermentation. The undigested fraction retained in the colon was analysed regarding its potential antioxidant, antimicrobial and prebiotic effects. LOPP and POPP did not impact the gut microbiota diversity negatively, showing a similar ratio of Firmicutes/Bacteroidetes compared to a positive control (FOS). LOPP exhibit a positive (similar to FOS) effect on the Prevotella spp./Bacteroides spp. ratio. Both powders also promoted more the production of short-chain fatty acids (mainly acetate?>?butyrate?>?propionate) than FOS. Both powders showed also significant total phenolic content and oxygen radical absorbance capacity during faecal fermentation until 48?h. Besides that, these powders showed mucin-adhesion inhibition ability against pathogens, principally POPP against Bacillus cereus (22.03?±?2.45%) and Listeria monocytogenes (20.01?±?1.93%). This study demonstrates that olive pomace powders have prebiotic effects on microbiota, including the stimulation of short-chain fatty acids production, potential antioxidant and antimicrobial activity which could improve human gut health.Tˆania I. B. Ribeiro thanks the FCT - Fundação para a Ciência e Tecnologia, Portugal and Association BLC3 – Technology and Innovation Campus, Centre Bio R&D Unit for the PhD Grant SFRH/BDE/108271/2015. This work was supported by National Funds from FCT - Fundação para a Ciência e a Tecnologia, Portugal through the project MULTI-BIOREFINERY - SAICTPAC/0040/2015 (POCI-01-0145-FEDER- 016403). We would also like to thank the scientific collaboration under the FCT project UID/Multi/50016/2019.info:eu-repo/semantics/publishedVersio
Phenolic Profiling of Olives and Olive Oil Process-Derived Matrices Using UPLC-DAD-ESI-QTOF-HRMS Analysis
All of the matrices
entailed in olive oil processing were screened
for the presence of known and new phenol constituents in a single
study, combining an ultra high pressure liquid chromatography system
with diode array and electrospray ionization quadrupole time-of-flight
high resolution mass spectrometry (ESI-QTOF-HRMS) detection. Their
trail was followed from the fruit (peel/pulp and stone) to the paste
and final products, i.e. pomace, wastewater, and oil, providing important
insight into the origin, disappearance, and evolution of each during
the operational steps. Eighty different phenols, composed of fruit
native representatives and their technologically formed and/or released
derivatives, were detected in six olive matrices and fully characterized
on the basis of HRMS and UV–vis spectroscopic data. In addition
to phenols already known in olive matrices, four new molecular formulas
were proposed and three new tentative identities assigned to newly
discovered phenols, i.e., β-methyl-OH-verbascoside, methoxynüzhenide,
and methoxynüzhenide 11-methyl oleoside
Olive fruit phenols transfer, transformation, and partition trail during laboratory-scale olive oil processing
This work is the most comprehensive study on the quantitative behavior of olive fruit phenols during olive oil processing, providing insight into their transfer, transformation, and partition trail. In total, 69 phenols were quantified in 6 olive matrices from a three-phase extraction line employing ultra high pressure liquid chromatography-diode array detection analysis. Crushing had a larger effect than malaxation in terms of phenolic degradation and transformation, resulting in several new evolutions of respective derivatives. The peel and pulp together confined 95% of total fruit phenols, while stone only 5%. However, only 0.53% of all ended-up in olive oil, nearly 6% in wastewater, and 48% in pomace. Secoiridoids were the predominant class in all matrices, though represented by different individuals. Their partition behavior was rather similar to other phenolic classes, where with few minor exceptions only aglycones were partitioned to the oil, while other glycosides were lost with the wastes. \ua9 2015 American Chemical Society