Índices de precios de la zona noroccidental de Castilla y León, 1518-1650

Editada en la Fundación Empresa PúblicaPublicad

Ovarian transcriptomic analysis reveals differential expression genes associated with cell death process after selection for ovulation rate in rabbits

[EN] Transcriptomic analysis showed nineteen potential biomarkers in ovarian tissue from females belonged to a rabbit line selected for ovulation rate for 10 generations and the control line. These females differed not only in ovulation rate but also in prenatal survival since similar litter size were observed. Litter size is an essential trait in rabbit meat production but with low heritability. A selection experiment for ovulation rate has been performed for 10 generations to improve litter size in rabbits. The selected line increased two ova more than the control line but nevertheless a negative correlation was observed with prenatal survival. A transcriptomic study was performed, using microarrays, in ovarian tissue from females belonging to the selected line and the control line. Our results showed 1357 differential expressed genes and nineteen potential biomarkers associated with prenatal mortality, which could explain differences between litter size in rabbits. Cell death was the most relevant process.This research was supported by MEC (AGL2014-55921-C2-1-P) and Generalitat Valenciana (Prometeo 2009/125).Serna-García, M.; Peiró Barber, RM.; Serna, E.; Santacreu Jerez, MA. (2020). Ovarian transcriptomic analysis reveals differential expression genes associated with cell death process after selection for ovulation rate in rabbits. Animals. 10(10):1-11. https://doi.org/10.3390/ani10101924S1111010Laborda, P., Mocé, M. L., Blasco, A., & Santacreu, M. A. (2012). Selection for ovulation rate in rabbits: Genetic parameters and correlated responses on survival rates1. Journal of Animal Science, 90(2), 439-446. doi:10.2527/jas.2011-4219Laborda, P., Mocé, M. L., Santacreu, M. A., & Blasco, A. (2011). Selection for ovulation rate in rabbits: Genetic parameters, direct response, and correlated response on litter size1. Journal of Animal Science, 89(10), 2981-2987. doi:10.2527/jas.2011-3906Laborda, P., Santacreu, M. A., Blasco, A., & Mocé, M. L. (2012). Selection for ovulation rate in rabbits: Direct and correlated responses estimated with a cryopreserved control population1. Journal of Animal Science, 90(10), 3392-3397. doi:10.2527/jas.2011-4837Cunningham, P. J., England, M. E., Young, L. D., & Zimmerman, D. R. (1979). Selection for Ovulation Rate in Swine: Correlated Response in Litter Size and Weight. Journal of Animal Science, 48(3), 509-516. doi:10.2527/jas1979.483509xRosendo, A., Druet, T., Gogué, J., & Bidanel, J. P. (2007). Direct responses to six generations of selection for ovulation rate or prenatal survival in Large White pigs. Journal of Animal Science, 85(2), 356-364. doi:10.2527/jas.2006-507Johnson, R. K., Zimmerman, D. R., & Kittok, R. J. (1984). Selection for components of reproduction in swine. Livestock Production Science, 11(6), 541-558. doi:10.1016/0301-6226(84)90070-8Rodrigues, P., Limback, D., McGinnis, L. K., Plancha, C. E., & Albertini, D. F. (2008). Oogenesis: Prospects and challenges for the future. Journal of Cellular Physiology, 216(2), 355-365. doi:10.1002/jcp.21473Cartuche, L., Pascual, M., Gómez, E. A., & Blasco, A. (2014). Economic weights in rabbit meat production. World Rabbit Science, 22(3), 165. doi:10.4995/wrs.2014.1747Zuelke, K. A., Jeffay, S. C., Zucker, R. M., & Perreault, S. D. (2002). Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre-implantation stage embryos. Molecular Reproduction and Development, 64(1), 106-112. doi:10.1002/mrd.10214Tiwari, M., Prasad, S., Tripathi, A., Pandey, A. N., Ali, I., Singh, A. K., … Chaube, S. K. (2015). Apoptosis in mammalian oocytes: a review. Apoptosis, 20(8), 1019-1025. doi:10.1007/s10495-015-1136-yGerritsen, M. E., & Wagner, G. F. (2005). Stanniocalcin: No Longer Just a Fish Tale. Vitamins & Hormones, 105-135. doi:10.1016/s0083-6729(05)70004-2Jepsen, M. R., Kløverpris, S., Bøtkjær, J. A., Wissing, M. L., Andersen, C. Y., & Oxvig, C. (2016). The proteolytic activity of pregnancy-associated plasma protein-A is potentially regulated by stanniocalcin-1 and -2 during human ovarian follicle development. Human Reproduction, 31(4), 866-874. doi:10.1093/humrep/dew013Darcy, C. J., Davis, J. S., Woodberry, T., McNeil, Y. R., Stephens, D. P., Yeo, T. W., & Anstey, N. M. (2011). An Observational Cohort Study of the Kynurenine to Tryptophan Ratio in Sepsis: Association with Impaired Immune and Microvascular Function. PLoS ONE, 6(6), e21185. doi:10.1371/journal.pone.0021185Wirthgen, E., Tuchscherer, M., Otten, W., Domanska, G., Wollenhaupt, K., Tuchscherer, A., & Kanitz, E. (2013). Activation of indoleamine 2,3-dioxygenase by LPS in a porcine model. Innate Immunity, 20(1), 30-39. doi:10.1177/1753425913481252Mohib, K., Guan, Q., Diao, H., Du, C., & Jevnikar, A. M. (2007). Proapoptotic activity of indoleamine 2,3-dioxygenase expressed in renal tubular epithelial cells. American Journal of Physiology-Renal Physiology, 293(3), F801-F812. doi:10.1152/ajprenal.00044.2007Fallarino, F., Grohmann, U., Vacca, C., Bianchi, R., Orabona, C., Spreca, A., … Puccetti, P. (2002). T cell apoptosis by tryptophan catabolism. Cell Death & Differentiation, 9(10), 1069-1077. doi:10.1038/sj.cdd.4401073Wang, Q., Zhang, M., Ding, Y., Wang, Q., Zhang, W., Song, P., & Zou, M.-H. (2014). Activation of NAD(P)H Oxidase by Tryptophan-Derived 3-Hydroxykynurenine Accelerates Endothelial Apoptosis and Dysfunction In Vivo. Circulation Research, 114(3), 480-492. doi:10.1161/circresaha.114.302113Li, F., Zhang, R., Li, S., & Liu, J. (2017). IDO1: An important immunotherapy target in cancer treatment. International Immunopharmacology, 47, 70-77. doi:10.1016/j.intimp.2017.03.024Hill, M., Pereira, V., Chauveau, C., Zagani, R., Remy, S., Tesson, L., … Anegon, I. (2005). Heme oxygenase‐1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2,3‐dioxygenase. The FASEB Journal, 19(14), 1957-1968. doi:10.1096/fj.05-3875comLieuallen, K. (2001). Cystatin B-deficient mice have increased expression of apoptosis and glial activation genes. Human Molecular Genetics, 10(18), 1867-1871. doi:10.1093/hmg/10.18.1867Pajaniappan, M., Glober, N. K., Kennard, S., Liu, H., Zhao, N., & Lilly, B. (2011). Endothelial cells downregulate apolipoprotein D expression in mural cells through paracrine secretion and Notch signaling. American Journal of Physiology-Heart and Circulatory Physiology, 301(3), H784-H793. doi:10.1152/ajpheart.00116.2011Duffy, D. M., Ko, C., Jo, M., Brannstrom, M., & Curry, T. E. (2018). Ovulation: Parallels With Inflammatory Processes. Endocrine Reviews, 40(2), 369-416. doi:10.1210/er.2018-00075LIU, C., LIU, Y., LIU, Y., WU, D., LUAN, Z., WANG, E., & YU, B. (2013). Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos. Molecular Medicine Reports, 7(6), 1929-1937. doi:10.3892/mmr.2013.1437Han, S. J., Chen, R., Paronetto, M. P., & Conti, M. (2005). Wee1B Is an Oocyte-Specific Kinase Involved in the Control of Meiotic Arrest in the Mouse. Current Biology, 15(18), 1670-1676. doi:10.1016/j.cub.2005.07.056Nakanishi, M., Ando, H., Watanabe, N., Kitamura, K., Ito, K., Okayama, H., … Sasaki, M. (2000). Identification and characterization of human Wee1B, a new member of the Wee1 family of Cdk-inhibitory kinases. Genes to Cells, 5(10), 839-847. doi:10.1046/j.1365-2443.2000.00367.xOh, J. S., Susor, A., & Conti, M. (2011). Protein Tyrosine Kinase Wee1B Is Essential for Metaphase II Exit in Mouse Oocytes. Science, 332(6028), 462-465. doi:10.1126/science.1199211Castedo, M., Perfettini, J.-L., Roumier, T., & Kroemer, G. (2002). Cyclin-dependent kinase-1: linking apoptosis to cell cycle and mitotic catastrophe. Cell Death & Differentiation, 9(12), 1287-1293. doi:10.1038/sj.cdd.4401130Golsteyn, R. M. (2005). Cdk1 and Cdk2 complexes (cyclin dependent kinases) in apoptosis: a role beyond the cell cycle. Cancer Letters, 217(2), 129-138. doi:10.1016/j.canlet.2004.08.005Gu, L., Zheng, H., Murray, S. A., Ying, H., & Jim Xiao, Z.-X. (2003). Deregulation of Cdc2 kinase induces caspase-3 activation and apoptosis. Biochemical and Biophysical Research Communications, 302(2), 384-391. doi:10.1016/s0006-291x(03)00189-xSandal, T., Stapnes, C., Kleivdal, H., Hedin, L., & Døskeland, S. O. (2002). A Novel, Extraneuronal Role for Cyclin-dependent Protein Kinase 5 (CDK5). Journal of Biological Chemistry, 277(23), 20783-20793. doi:10.1074/jbc.m112248200Oh, J. S., Susor, A., Schindler, K., Schultz, R. M., & Conti, M. (2013). Cdc25A activity is required for the metaphase II arrest in mouse oocytes. Journal of Cell Science. doi:10.1242/jcs.115592Orciani, M., Trubiani, O., Guarnieri, S., Ferrero, E., & Di Primio, R. (2008). CD38 is constitutively expressed in the nucleus of human hematopoietic cells. Journal of Cellular Biochemistry, 105(3), 905-912. doi:10.1002/jcb.21887Partidá-Sánchez, S., Rivero-Nava, L., Shi, G., & Lund, F. E. (s. f.). CD38: An Ecto-Enzyme at the Crossroads of Innate and Adaptive Immune Responses. Crossroads between Innate and Adaptive Immunity, 171-183. doi:10.1007/978-0-387-34814-8_12Wang, L.-F., Miao, L.-J., Wang, X.-N., Huang, C.-C., Qian, Y.-S., Huang, X., … Xin, H.-B. (2017). CD38 deficiency suppresses adipogenesis and lipogenesis in adipose tissues through activating Sirt1/PPARγ signaling pathway. Journal of Cellular and Molecular Medicine, 22(1), 101-110. doi:10.1111/jcmm.13297Sun, L., Iqbal, J., Zaidi, S., Zhu, L.-L., Zhang, X., Peng, Y., … Zaidi, M. (2006). Structure and functional regulation of the CD38 promoter. Biochemical and Biophysical Research Communications, 341(3), 804-809. doi:10.1016/j.bbrc.2006.01.033Uche, U. U., Piccirillo, A. R., Kataoka, S., Grebinoski, S. J., D’Cruz, L. M., & Kane, L. P. (2018). PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. Journal of Experimental Medicine, 215(12), 3165-3179. doi:10.1084/jem.20172018Chu, K. Y., Li, H., Wada, K., & Johnson, J. D. (2011). Ubiquitin C-terminal hydrolase L1 is required for pancreatic beta cell survival and function in lipotoxic conditions. Diabetologia, 55(1), 128-140. doi:10.1007/s00125-011-2323-1Xiang, T., Li, L., Yin, X., Yuan, C., Tan, C., Su, X., … Tao, Q. (2012). The Ubiquitin Peptidase UCHL1 Induces G0/G1 Cell Cycle Arrest and Apoptosis Through Stabilizing p53 and Is Frequently Silenced in Breast Cancer. PLoS ONE, 7(1), e29783. doi:10.1371/journal.pone.0029783Kabuta, T., Mitsui, T., Takahashi, M., Fujiwara, Y., Kabuta, C., Konya, C., … Wada, K. (2013). Ubiquitin C-terminal Hydrolase L1 (UCH-L1) Acts as a Novel Potentiator of Cyclin-dependent Kinases to Enhance Cell Proliferation Independently of Its Hydrolase Activity. Journal of Biological Chemistry, 288(18), 12615-12626. doi:10.1074/jbc.m112.435701Koyanagi, S., Hamasaki, H., Sekiguchi, S., Hara, K., Ishii, Y., Kyuwa, S., & Yoshikawa, Y. (2012). Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova. REPRODUCTION, 143(3), 271-279. doi:10.1530/rep-11-0128Yao, Y.-W., Shi, Y., Jia, Z.-F., Jiang, Y.-H., Gu, Z., Wang, J., … Sun, Z.-G. (2011). PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development. Histochemistry and Cell Biology, 136(2), 205-215. doi:10.1007/s00418-011-0825-zBoelte, K. C., Gordy, L. E., Joyce, S., Thompson, M. A., Yang, L., & Lin, P. C. (2011). Rgs2 Mediates Pro-Angiogenic Function of Myeloid Derived Suppressor Cells in the Tumor Microenvironment via Upregulation of MCP-1. PLoS ONE, 6(4), e18534. doi:10.1371/journal.pone.0018534Schwameis, M., Blann, A., Mannhalter, C., Jilma, B., & Siller-Matula, J. (2011). Thrombin as a multi-functional enzyme. Thrombosis and Haemostasis, 106(12), 1020-1033. doi:10.1160/th10-11-0711Van Blerkom, J., Antczak, M., & Schrader, R. (1997). The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics. Human Reproduction, 12(5), 1047-1055. doi:10.1093/humrep/12.5.1047Richards, J. S., Liu, Z., Kawai, T., Tabata, K., Watanabe, H., Suresh, D., … Shimada, M. (2012). Adiponectin and its receptors modulate granulosa cell and cumulus cell functions, fertility, and early embryo development in the mouse and human. Fertility and Sterility, 98(2), 471-479.e1. doi:10.1016/j.fertnstert.2012.04.050Lagaly, D. V., Aad, P. Y., Grado-Ahuir, J. A., Hulsey, L. B., & Spicer, L. J. (2008). Role of adiponectin in regulating ovarian theca and granulosa cell function. Molecular and Cellular Endocrinology, 284(1-2), 38-45. doi:10.1016/j.mce.2008.01.007Palin, M.-F., Bordignon, V. V., & Murphy, B. D. (2012). Adiponectin and the Control of Female Reproductive Functions. Vitamins & Hormones, 239-287. doi:10.1016/b978-0-12-398313-8.00010-5Wickham, E. P., Tao, T., Nestler, J. E., & McGee, E. A. (2013). Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells. Journal of Assisted Reproduction and Genetics, 30(7), 963-968. doi:10.1007/s10815-013-0012-3Chappaz, E., Albornoz, M. S., Campos, D., Che, L., Palin, M.-F., Murphy, B. D., & Bordignon, V. (2008). Adiponectin enhances in vitro development of swine embryos. Domestic Animal Endocrinology, 35(2), 198-207. doi:10.1016/j.domaniend.2008.05.007Elis, S., Coyral-Castel, S., Freret, S., Cognié, J., Desmarchais, A., Fatet, A., … Dupont, J. (2013). Expression of adipokine and lipid metabolism genes in adipose tissue of dairy cows differing in a female fertility quantitative trait locus. Journal of Dairy Science, 96(12), 7591-7602. doi:10.3168/jds.2013-6615Bovolenta, P., Esteve, P., Ruiz, J. M., Cisneros, E., & Lopez-Rios, J. (2008). Beyond Wnt inhibition: new functions of secreted Frizzled-related proteins in development and disease. Journal of Cell Science, 121(6), 737-746. doi:10.1242/jcs.026096Arslanoglu, S., Bertino, E., Tonetto, P., De Nisi, G., Ambruzzi, A. M., Biasini, A., … Moro, G. E. (2010). Guidelines for the establishment and operation of a donor human milk bank. The Journal of Maternal-Fetal & Neonatal Medicine, 23(sup2), 1-20. doi:10.3109/14767058.2010.512414LIN, C.-T., LIN, Y.-T., & KUO, T.-F. (2007). Investigation of mRNA Expression for Secreted Frizzled-Related Protein 2 (sFRP2) in Chick Embryos. Journal of Reproduction and Development, 53(4), 801-810. doi:10.1262/jrd.18081Jaatinen, R., Bondestam, J., Raivio, T., Hildén, K., Dunkel, L., Groome, N., & Ritvos, O. (2002). Activation of the Bone Morphogenetic Protein Signaling Pathway Induces Inhibin βB-Subunit mRNA and Secreted Inhibin B Levels in Cultured Human Granulosa-Luteal Cells. The Journal of Clinical Endocrinology & Metabolism, 87(3), 1254-1261. doi:10.1210/jcem.87.3.8314De Gottardi, A., Dumonceau, J.-M., Bruttin, F., Vonlaufen, A., Morard, I., Spahr, L., … Hadengue, A. (2006). Molecular Cancer, 5(1), 48. doi:10.1186/1476-4598-5-48LUTWAK-MANN, C. (1955). CARBONIC ANHYDRASE IN THE FEMALE REPRODUCTIVE TRACT. OCCURRENCE, DISTRIBUTION AND HORMONAL DEPENDENCE. Journal of Endocrinology, 13(1), 26-38. doi:10.1677/joe.0.013002

Does waste feed from salmon farming affect the quality of saithe (Pollachius virens L.) attracted to fish farms?

Salmon farms attract large amounts of wild fish, which prey on uneaten feed pellets. The modified diet of the wild fish aggregating at salmon farms may reduce the flesh quality of the fish, influencing the local fisheries. We compared the quality of saithe (Pollachius virens) captured near (farm associated—FA) or more than 5 km away (un‐associated—UA) from salmon farms in Norway. The fish were captured during summer, autumn and spring using two commercial fishing methods (jigging and bottom nets). Overall, the fillet quality of FA saithe was good, although it was clearly reduced for almost 10% of the catch. Moreover, the quality of the FA saithe was significantly reduced compared with UA saithe, but the differences were small. Our results also showed that fish caught with jigging had better quality than fish caught with nets, and that fish that died in the nets were of lower quality than fish that were alive after hauling. There was no clear variation among seasons in fillet quality. Although no major and overall differences in quality were found between FA and UA saithe, reduced quality for even a modest proportion of the fish may influence the value of the total catch.The study was funded by the Norwegian Seafood Research Fund through the ProCoEx project (Project number: 900772) and the Norwegian Research Council through the project ‘ECOCOAST’. Kilian Toledo‐Guedes was supported by a grant from Iceland, Liechtenstein and Norway through the EEA Financial Mechanism (ES07‐EEA Grants, 013‐ABEL‐IM‐2013), operated by Universidad Complutense de Madrid, and Juan de la Cierva Program FJCI‐2014‐20100 and IJCI‐2017‐34174 from Ministerio de Ciencia, Innovación y Universidades

Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness and enhanced osteoblast differentiation for biomedical applications

Ti-based alloys have increased importance for biomedical applications due to their excellent properties. In particular, the two recently developed TiZrPdSi(Nb) alloys, with a predominant β-Ti phase microstructure, have good mechanical properties, such as a relatively low Young's modulus and high hardness. In the present work, the cytocompatibility of these alloys was assessed using human osteoblast-like Saos-2 cells. Cells grown on the alloys showed larger spreading areas (more than twice) and higher vinculin content (nearly 40% increment) when compared with cells grown on glass control surfaces, indicating a better cell adhesion. Moreover, cell proliferation was 18% higher for cells growing on both alloys than for cells growing on glass and polystyrene control surfaces. Osteogenic differentiation was evaluated by quantifying the expression of four osteogenic genes (osteonectin, osteocalcin, osteopontin, and bone sialoprotein), the presence of three osteogenic proteins (alkaline phosphatase, collagen I, and osteocalcin) and the activity of alkaline phosphatase at different time-points. The results demonstrated that TiZrPdSi and TiZrPdSiNb alloys enhance osteoblast differentiation, and that cells grown on TiZrPdSiNb alloy present higher levels of some late osteogenic markers during the first week in culture. These results suggest that the TiZrPdSi(Nb) alloys can be considered as excellent candidates for orthopaedical uses

ZnO Nanosheet-Coated TiZrPdSiNb Alloy as a Piezoelectric Hybrid Material for Self-Stimulating Orthopedic Implants

Altres ajuts: This work has been partially supported by La Caixa Foundation under the Junior Leader Retaining program ( LCF/BQ/PR19/11700010).A Ti-based alloy (Ti45Zr15Pd30Si5Nb5) with already proven excellent mechanical and biocompatibility features has been coated with piezoelectric zinc oxide (ZnO) to induce the electrical self-stimulation of cells. ZnO was grown onto the pristine alloy in two different morphologies: a flat dense film and an array of nanosheets. The effect of the combined material on osteoblasts (electrically stimulable cells) was analyzed in terms of proliferation, cell adhesion, expression of differentiation markers and induction of calcium transients. Although both ZnO structures were biocompatible and did not induce inflammatory response, only the array of ZnO nanosheets was able to induce calcium transients, which improved the proliferation of Saos-2 cells and enhanced the expression of some early differentiation expression genes. The usual motion of the cells imposes strain to the ZnO nanosheets, which, in turn, create local electric fields owing to their piezoelectric character. These electric fields cause the opening of calcium voltage gates and boost cell proliferation and early differentiation. Thus, the modification of the Ti45Zr15Pd30Si5Nb5 surface with an array of ZnO nanosheets endows the alloy with smart characteristics, making it capable of electric self-stimulation

Fitted avatars: automatic skeleton adjustment for self-avatars in virtual reality

In the era of the metaverse, self-avatars are gaining popularity, as they can enhance presence and provide embodiment when a user is immersed in Virtual Reality. They are also very important in collaborative Virtual Reality to improve communication through gestures. Whether we are using a complex motion capture solution or a few trackers with inverse kinematics (IK), it is essential to have a good match in size between the avatar and the user, as otherwise mismatches in self-avatar posture could be noticeable for the user. To achieve such a correct match in dimensions, a manual process is often required, with the need for a second person to take measurements of body limbs and introduce them into the system. This process can be time-consuming, and prone to errors. In this paper, we propose an automatic measuring method that simply requires the user to do a small set of exercises while wearing a Head-Mounted Display (HMD), two hand controllers, and three trackers. Our work provides an affordable and quick method to automatically extract user measurements and adjust the virtual humanoid skeleton to the exact dimensions. Our results show that our method can reduce the misalignment produced by the IK system when compared to other solutions that simply apply a uniform scaling to an avatar based on the height of the HMD, and make assumptions about the locations of joints with respect to the trackers.This work was funded by the Spanish Ministry of Science and Innovation (PID2021-122136OB-C21). Jose Luis Ponton was also funded by the Spanish Ministry of Universities (FPU21/01927).Peer ReviewedPostprint (published version

A step towards the validation of bacteria biotic indices using DNA metabarcoding for benthic monitoring

Environmental genomics is a promising field for monitoring biodiversity in a timely fashion. Efforts have increasingly been dedicated to the use of bacteria DNA derived data to develop biotic indices for benthic monitoring. However, a substantial debate exists about whether bacteria‐derived data using DNA metabarcoding should follow, for example, a taxonomy‐based or a taxonomy‐free approach to marine bioassessments. Here, we showcase the value of DNA‐based monitoring using the impact of fish farming as an example of anthropogenic disturbances in coastal areas and compare the performance of taxonomy‐based and taxonomy‐free approaches in detecting environmental alterations. We analysed samples collected near to the farm cages and along distance gradients from two aquaculture installations, and at control sites, to evaluate the effect of this activity on bacterial assemblages. Using the putative response of bacterial taxa to stress we calculated the taxonomy‐based biotic index microgAMBI. The distribution of individual amplicon sequence variants (ASVs), as a function of a gradient in sediment acid volatile sulphides, was then used to derive a taxonomy‐free bacterial biotic index specific for this data set using a de novo approach based on quantile regression splines. Our results show that microgAMBI revealed a organically enriched environment along the gradient. However, the de novo biotic index outperformed microgAMBI by providing a higher discriminatory power in detecting changes in abiotic factors directly related to fish production, whilst allowing the identification of new ASVs bioindicators. The de novo strategy applied here represents a robust method to define new bioindicators in regions or habitats where no previous information about the response of bacteria to environmental stressors exists.This work was partially funded by the project CGL2015-70136-R from the Spanish Ministry of Economy and Competitiveness (MINECO) and the EU ERDF funding program. E.A. and S.C. are supported by funding from a collaboration between KAUST and Saudi Aramco within the framework of the Saudi Aramco –KAUST Center for Marine Environmental Observations. K.T.-G. is supported by Ministerio de Ciencia, Innnovación y Universidades through the Juan de la Cierva Incorporación program (IJCI-2017-34174)

Presencia física de profesionales de Salud Mental en un Centro de Atención Primaria como forma alternativa de coordinación. Una experiencia piloto.

Introduccion: problemas de coordinación entre los niveles de atención primaria y salud mental sugieren la necesidad de explorar nuevas vías que faciliten la comunicación entre ambos niveles asistenciales. Material y Método: Un Psiquiatra y un Psicólogo Clínico de Alcalá de Henares (Área 3 de la Comunidad Autónoma de Madrid) se han desplazado un día a la semana a un centro de atención primaria (centro experimental) para evaluar pacientes derivados por los médicos de ese centro y contrastar directamente con ellos sus valoraciones. Después de 15 meses de funcionamiento, se administró a los médicos un cuestionario acerca de su satisfacción con su relación con salud mental. Sus respuestas se compararon con las de médicos de otro centro en el que no tenía lugar la experiencia (centro control). Resultados: los médicos del centro experimental valoraban mejor la información recibida por salud mental y la cantidad de contactos que tenían con ellos, percibían al equipo de salud mental como más disponible, recibían más cursos específicos, se habían coordinado más con salud mental y, en general, valoraban mejor la coordinación atención primaria – salud mental que los del centro control. Conclusiones: La experiencia muestra cómo es posible una forma alternativa de coordinación con la que los médicos de primaria se muestran altamente satisfechos. Se plantean algunas cuestiones metodológicas y posibles vías para continuar futuros trabajos

Impact of printing orientation on inter and intra-layer bonds in 3D printed thermoplastic elastomers: a study using acoustic emission and tensile tests

The use of the Material Extrusion technique with Thermoplastic Elastomers is currently growing because of the large number of benefits of this family of materials. They are processable materials with high flexibility, which makes them very useful, for example, in biomedical applications that require flexible objects with complex geometries. This study aims to characterize a specific polymer, namely polyether-block-amide-based polymer (PEBA), by analyzing its anisotropic behavior in printed samples and investigating the mechanical properties based on different printing orientations. Three orientations (X, Y, and Z) were used to relate the printing orientation to the type of bonds formed in the samples: intra-layer bonds, inter-layer bonds, and the deposited filament. Tensile tests following ASTM D638 were conducted to measure sample rigidity, while Acoustic Emission, an advanced Non-Destructive Technique, was employed to examine the trend of the failure process. The presence of voids was also observed to assess printing quality, which is influenced by the printing orientation and alters the quality of bonds. The results revealed that samples printed horizontally exhibited higher Young’s Modulus values and fewer voids in the inner parts. Vertically printed samples displayed inferior mechanical properties and a greater number of voids. Consequently, the intra-layer yielded better bond formation and minimized voids. Acoustic Emission analysis corroborated these findings by demonstrating distinct energy distribution patterns among the different printing orientations. Hits were concentrated at maximum stresses, primarily observed in the vertically printed samples, which experienced macroscopic failure. Furthermore, this particular specimen exhibited a vertical asymptote near the maximum stress level. The analysis of the energy of Acoustic Emission hits demonstrated a reasonably good fit with the Gutenberg-Richter (GBR) law based on the printing direction.Peer ReviewedObjectius de Desenvolupament Sostenible::9 - Indústria, Innovació i InfraestructuraPostprint (published version