Academic Majors of Upperclassmen Football Players in the Atlantic Coast Conference: An Analysis of Academic Clustering Comparing White and Minority Players

Studies on graduation rates of college athletes have typically utilized subdivisions based on race, gender, and sport to allow for more transparent scrutiny of potential problem areas. However, subdivision by race has not been utilized when examining clustering of football players into academic majors. Clustering occurs when 25% or more of an athletic team shares a single academic major (Case, Greer, & Brown, 1987). Football media guides from Atlantic Coast Conference (ACC) schools were utilized to determine the academic majors of upperclassmen to explore whether race could be a factor in academic clustering. The results showed that Minority players were clustered into specific academic programs at greater rates than their White counterparts. While academic clustering occurred for both White and Minority players, the clustering of Minority players involved greater numbers of players enrolled in clustered majors, as well as the existence of a second clustered major at several schools. At six of the schools in the study, 75% or more of the Minority players were enrolled into just two academic majors

Nitric oxide inhibits the accumulation of CD4+CD44hiTbet+CD69lo T cells in mycobacterial infection

Animals lacking the inducible nitric oxide synthase gene (nos2-/-) are less susceptible to Mycobacterium avium strain 25291 and lack nitric oxide-mediated immunomodulation of CD4+ T cells. Here we show that the absence of nos2 results in increased accumulation of neutrophils and both CD4+ and CD8+ T cells within the M. avium containing granuloma. Examination of the T-cell phenotype in M. avium infected mice demonstrated that CD4+CD44hi effector T cells expressing the Th1 transcriptional regulator T-bet (T-bet+) were specifically reduced by the presence of nitric oxide. Importantly, the T-bet+ effector population could be separated into CD69hi and CD69lo populations, with the CD69lo population only able to accumulate during chronic infection within infected nos2-/- mice. Transcriptomic comparison between CD4+CD44hiCD69hi and CD4+CD44hiCD69lo populations revealed that CD4+CD44hiCD69lo cells had higher expression of the integrin itgb1/itga4 (VLA-4, CD49d/CD29). Inhibition of Nos2 activity allowed increased accumulation of the CD4+CD44hiT-bet+CD69lo population in WT mice as well as increased expression of VLA-4. These data support the hypothesis that effector T cells in mycobacterial granulomata are not a uniform effector population but exist in distinct subsets with differential susceptibility to the regulatory effects of nitric oxide

Discovery of Novel MicroRNAs in Female Reproductive Tract Using Next Generation Sequencing

MicroRNAs (miRNAs) are small non-coding RNAs that mediate post-transcriptional gene silencing. Over 700 human miRNAs have currently been identified, many of which are mutated or de-regulated in diseases. Here we report the identification of novel miRNAs through deep sequencing the small RNAome (<30 nt) of over 100 tissues or cell lines derived from human female reproductive organs in both normal and disease states. These specimens include ovarian epithelium and ovarian cancer, endometrium and endometriomas, and uterine myometrium and uterine smooth muscle tumors. Sequence reads not aligning with known miRNAs were each mapped to the genome to extract flanking sequences. These extended sequence regions were folded in silico to identify RNA hairpins. Sequences demonstrating the ability to form a stem loop structure with low minimum free energy (<−25 kcal) and predicted Drosha and Dicer cut sites yielding a mature miRNA sequence matching the actual sequence were considered putative novel miRNAs. Additional confidence was achieved when putative novel hairpins assembled a collection of sequences highly similar to the putative mature miRNA but with heterogeneous 3′-ends. A confirmed novel miRNA fulfilled these criteria and had its “star” sequence in our collection. We found 7 distinct confirmed novel miRNAs, and 51 additional novel miRNAs that represented highly confident predictions but without detectable star sequences. Our novel miRNAs were detectable in multiple samples, but expressed at low levels and not specific to any one tissue or cell type. To date, this study represents the largest set of samples analyzed together to identify novel miRNAs

Pathological role of interleukin 17 in mice subjected to repeated BCG vaccination after infection with Mycobacterium tuberculosis

Infection usually leads to the development of acquired immune responses associated with clearance or control of the infecting organism. However, if not adequately regulated, immune-mediated pathology can result. Tuberculosis is a worldwide threat, and development of an effective vaccine requires that the protective immune response to Mycobacterium tuberculosis (Mtb) be dissected from the pathological immune response. This distinction is particularly important if new vaccines are to be delivered to Mtb-exposed individuals, as repeated antigenic exposure can lead to pathological complications. Using a model wherein mice are vaccinated with bacille Calmette-Guérin after Mtb infection, we show that repeated vaccination results in increased IL-17, tumor necrosis factor, IL-6, and MIP-2 expression, influx of granulocytes/neutrophils, and lung tissue damage. This pathological response is abrogated in mice deficient in the gene encoding IL-23p19 or in the presence of IL-17–blocking antibody. This finding that repeated exposure to mycobacterial antigen promotes enhanced IL-17–dependent pathological consequences has important implications for the design of effective vaccines against Mtb

Mycobacterium tuberculosis infection induces il12rb1 splicing to generate a novel IL-12Rβ1 isoform that enhances DC migration

RNA splicing is an increasingly recognized regulator of immunity. Here, we demonstrate that after Mycobacterium tuberculosis infection (mRNA) il12rb1 is spliced by dendritic cells (DCs) to form an alternative (mRNA) il12rb1Deltatm that encodes the protein IL-12Rbeta1DeltaTM. Compared with IL-12Rbeta1, IL-12Rbeta1DeltaTM contains an altered C-terminal sequence and lacks a transmembrane domain. Expression of IL-12Rbeta1DeltaTM occurs in CD11c(+) cells in the lungs during M. tuberculosis infection. Selective reconstitution of il12rb1(-/-) DCs with (mRNA) il12rb1 and/or (mRNA) il12rb1Deltatm demonstrates that IL-12Rbeta1DeltaTM augments IL-12Rbeta1-dependent DC migration and activation of M. tuberculosis-specific T cells. It cannot mediate these activities independently of IL12Rbeta1. We hypothesize that M. tuberculosis-exposed DCs express IL-12Rbeta1DeltaTM to enhance IL-12Rbeta1-dependent migration and promote M. tuberculosis-specific T cell activation. IL-12Rbeta1DeltaTM thus represents a novel positive-regulator of IL12Rbeta1-dependent DC function and of the immune response to M. tuberculosis.This work was supported by the Trudeau Institute and the National Institutes of Health (AI067723 to A. M. Cooper; AI49823 to D. L. Woodland [trainee: R. T. Robinson] and AI084397 to R. T. Robinson)

An Examination of Race Strategies in NCAA Cross Country Championship Events

This research sought to evaluate race strategies in Cross Country running to determine whether a fast, predicted, or slow start would yield the best results when coupled with an advancing or regressing strategy from the 1st checkpoint through to the finish. Twelve National Collegiate Athletic Association (NCAA) Championship races were analyzed from 2021 to 2022 (N=315 teams; 2,205 runners). Success was defined by each team\u27s finish relative to their anticipated finish, as determined through pre-championship national rankings. Results indicated that starting well ahead of a predicted finishing place and advancing throughout the race can yield finishes well ahead of a team\u27s ranking. Still, less than 5% of teams could employ that strategy. A more likely positive result came from a conservative start, coupled with consistently advancing through the field for the remainder of the race, as nearly 14% of teams could employ that strategy. Starting a race slowly did not typically lead to success, even if a team consistently advanced through the field after the 1st checkpoint. Teams that regressed throughout the race were not likely to have a successful race

Academic Clustering: A Longitudinal Analysis of a Division I Football Program

As NCAA Division I coaches feel greater pressure to produce winning teams while ensuring that athletes remain eligible and progress toward degrees to avoid sanctions under the NCAA’s academic reform initiatives, concerns regarding the clustering of athletes into limited numbers of academic majors has increased. Academic clustering occurs when 25% or more of the members of one team share a single academic major (Case, Greer, & Brown, 1987). Recent studies have extended the analysis of clustering to include the disparate impact on white and minority football players in a single athletic conference (Fountain & Finley, 2009), as well as consideration of female basketball players throughout Division I (Paule, 2010). To date, these studies have provided a snapshot of teams for a given season. This study extends the understanding of clustering by examining one football program over a period of ten years, which allowed for greater understanding of the movement of players into and out of majors, especially the movement into a clustered major midway through their academic experience. Media guides from one BCS football program were used to track the listed majors of 349 players, from 2000 through 2009. Results indicated that players migrated into a single clustered major over time and that a significant number of touted recruits and National Football League draftees selected the clustered major. Further, players who had listed general education (University Studies) in their first media guide appearances frequently selected the clustered major