80 research outputs found
Soft Listeria: actin-based propulsion of liquid drops
We study the motion of oil drops propelled by actin polymerization in cell
extracts. Drops deform and acquire a pear-like shape under the action of the
elastic stresses exerted by the actin comet. We solve this free boundary
problem and calculate the drop shape taking into account the elasticity of the
actin gel and the variation of the polymerization velocity with normal stress.
The pressure balance on the liquid drop imposes a zero propulsive force if
gradients in surface tension or internal pressure are not taken into account.
Quantitative parameters of actin polymerization are obtained by fitting theory
to experiment.Comment: 5 pages, 4 figure
Mechanical Instabilities of Biological Tubes
We study theoretically the shapes of biological tubes affected by various
pathologies. When epithelial cells grow at an uncontrolled rate, the negative
tension produced by their division provokes a buckling instability. Several
shapes are investigated : varicose, enlarged, sinusoidal or sausage-like, all
of which are found in pathologies of tracheal, renal tubes or arteries. The
final shape depends crucially on the mechanical parameters of the tissues :
Young modulus, wall-to-lumen ratio, homeostatic pressure. We argue that since
tissues must be in quasistatic mechanical equilibrium, abnormal shapes convey
information as to what causes the pathology. We calculate a phase diagram of
tubular instabilities which could be a helpful guide for investigating the
underlying genetic regulation
Undulation Instability of Epithelial Tissues
Treating the epithelium as an incompressible fluid adjacent to a viscoelastic
stroma, we find a novel hydrodynamic instability that leads to the formation of
protrusions of the epithelium into the stroma. This instability is a candidate
for epithelial fingering observed in vivo. It occurs for sufficiently large
viscosity, cell-division rate and thickness of the dividing region in the
epithelium. Our work provides physical insight into a potential mechanism by
which interfaces between epithelia and stromas undulate, and potentially by
which tissue dysplasia leads to cancerous invasion.Comment: 4 pages, 3 figure
Mechanochemical action of the dynamin protein
Dynamin is a ubiquitous GTPase that tubulates lipid bilayers and is
implicated in many membrane severing processes in eukaryotic cells. Setting the
grounds for a better understanding of this biological function, we develop a
generalized hydrodynamics description of the conformational change of large
dynamin-membrane tubes taking into account GTP consumption as a free energy
source. On observable time scales, dissipation is dominated by an effective
dynamin/membrane friction and the deformation field of the tube has a simple
diffusive behavior, which could be tested experimentally. A more involved,
semi-microscopic model yields complete predictions for the dynamics of the tube
and possibly accounts for contradictory experimental results concerning its
change of conformation as well as for plectonemic supercoiling.Comment: 17 pages, 4 figures; typos corrected, reference adde
Real-Time Online Monitoring of the Ion Range by Means of Prompt Secondary Radiations
International audiencePrompt secondary radiations such as gamma rays and protons can be used for ion-range monitoring during ion therapy either on an energy-slice basis or on a pencil-beam basis. We present a review of the ongoing activities in terms of detector developments, imaging, experimental and theoretical physics issues concerning the correlation between the physical dose and hadronic processe
Damaged DNA Binding Protein 2 Plays a Role in Breast Cancer Cell Growth
The Damaged DNA binding protein 2 (DDB2), is involved in nucleotide excision repair as well as in other biological processes in normal cells, including transcription and cell cycle regulation. Loss of DDB2 function may be related to tumor susceptibility. However, hypothesis of this study was that DDB2 could play a role in breast cancer cell growth, resulting in its well known interaction with the proliferative marker E2F1 in breast neoplasia. DDB2 gene was overexpressed in estrogen receptor (ER)-positive (MCF-7 and T47D), but not in ER-negative breast cancer (MDA-MB231 and SKBR3) or normal mammary epithelial cell lines. In addition, DDB2 expression was significantly (3.0-fold) higher in ER-positive than in ER-negative tumor samples (P = 0.0208) from 16 patients with breast carcinoma. Knockdown of DDB2 by small interfering RNA in MCF-7 cells caused a decrease in cancer cell growth and colony formation. Inversely, introduction of the DDB2 gene into MDA-MB231 cells stimulated growth and colony formation. Cell cycle distribution and 5 Bromodeoxyuridine incorporation by flow cytometry analysis showed that the growth-inhibiting effect of DDB2 knockdown was the consequence of a delayed G1/S transition and a slowed progression through the S phase of MCF-7 cells. These results were supported by a strong decrease in the expression of S phase markers (Proliferating Cell Nuclear Antigen, cyclin E and dihydrofolate reductase). These findings demonstrate for the first time that DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer
Year in review in Intensive Care Medicine, 2008: II. Experimental, acute respiratory failure and ARDS, mechanical ventilation and endotracheal intubation
SCOPUS: re.jinfo:eu-repo/semantics/publishe
Year in review in Intensive Care Medicine 2011: III. ARDS and ECMO, weaning, mechanical ventilation, noninvasive ventilation, pediatrics and miscellanea
SCOPUS: re.jinfo:eu-repo/semantics/publishe
Rapid Dissemination of SIV Follows Multisite Entry after Rectal Inoculation
Receptive ano-rectal intercourse is a major cause of HIV infection in men having sex with men and in heterosexuals. Current knowledge of the mechanisms of entry and dissemination during HIV rectal transmission is scarce and does not allow the development of preventive strategies. We investigated the early steps of rectal infection in rhesus macaques inoculated with the pathogenic isolate SIVmac251 and necropsied four hours to nine days later. All macaques were positive for SIV. Control macaques inoculated with heat-inactivated virus were consistently negative for SIV. SIV DNA was detected in the rectum as early as four hours post infection by nested PCR for gag in many laser-microdissected samples of lymphoid aggregates and lamina propria but never in follicle-associated epithelium. Scarce SIV antigen positive cells were observed by immunohistofluorescence in the rectum, among intraepithelial and lamina propria cells as well as in clusters in lymphoid aggregates, four hours post infection and onwards. These cells were T cells and non-T cells that were not epithelial cells, CD68+ macrophages, DC-SIGN+ cells or fascin+ dendritic cells. DC-SIGN+ cells carried infectious virus. Detection of Env singly spliced mRNA in the mucosa by nested RT-PCR indicated ongoing viral replication. Strikingly, four hours post infection colic lymph nodes were also infected in all macaques as either SIV DNA or infectious virus was recovered. Rapid SIV entry and dissemination is consistent with trans-epithelial transport. Virions appear to cross the follicle-associated epithelium, and also the digestive epithelium. Viral replication could however be more efficient in lymphoid aggregates. The initial sequence of events differs from both vaginal and oral infections, which implies that prevention strategies for rectal transmission will have to be specific. Microbicides will need to protect both digestive and follicle-associated epithelia. Vaccines will need to induce immunity in lymph nodes as well as in the rectum
Hyperoxemia and excess oxygen use in early acute respiratory distress syndrome : Insights from the LUNG SAFE study
Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.Background: Concerns exist regarding the prevalence and impact of unnecessary oxygen use in patients with acute respiratory distress syndrome (ARDS). We examined this issue in patients with ARDS enrolled in the Large observational study to UNderstand the Global impact of Severe Acute respiratory FailurE (LUNG SAFE) study. Methods: In this secondary analysis of the LUNG SAFE study, we wished to determine the prevalence and the outcomes associated with hyperoxemia on day 1, sustained hyperoxemia, and excessive oxygen use in patients with early ARDS. Patients who fulfilled criteria of ARDS on day 1 and day 2 of acute hypoxemic respiratory failure were categorized based on the presence of hyperoxemia (PaO2 > 100 mmHg) on day 1, sustained (i.e., present on day 1 and day 2) hyperoxemia, or excessive oxygen use (FIO2 ≥ 0.60 during hyperoxemia). Results: Of 2005 patients that met the inclusion criteria, 131 (6.5%) were hypoxemic (PaO2 < 55 mmHg), 607 (30%) had hyperoxemia on day 1, and 250 (12%) had sustained hyperoxemia. Excess FIO2 use occurred in 400 (66%) out of 607 patients with hyperoxemia. Excess FIO2 use decreased from day 1 to day 2 of ARDS, with most hyperoxemic patients on day 2 receiving relatively low FIO2. Multivariate analyses found no independent relationship between day 1 hyperoxemia, sustained hyperoxemia, or excess FIO2 use and adverse clinical outcomes. Mortality was 42% in patients with excess FIO2 use, compared to 39% in a propensity-matched sample of normoxemic (PaO2 55-100 mmHg) patients (P = 0.47). Conclusions: Hyperoxemia and excess oxygen use are both prevalent in early ARDS but are most often non-sustained. No relationship was found between hyperoxemia or excessive oxygen use and patient outcome in this cohort. Trial registration: LUNG-SAFE is registered with ClinicalTrials.gov, NCT02010073publishersversionPeer reviewe
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