2,903 research outputs found
Structural basis of template-boundary definition in Tetrahymena telomerase.
Telomerase is required to maintain repetitive G-rich telomeric DNA sequences at chromosome ends. To do so, the telomerase reverse transcriptase (TERT) subunit reiteratively uses a small region of the integral telomerase RNA (TER) as a template. An essential feature of telomerase catalysis is the strict definition of the template boundary to determine the precise TER nucleotides to be reverse transcribed by TERT. We report the 3-Å crystal structure of the Tetrahymena TERT RNA-binding domain (tTRBD) bound to the template boundary element (TBE) of TER. tTRBD is wedged into the base of the TBE RNA stem-loop, and each of the flanking RNA strands wraps around opposite sides of the protein domain. The structure illustrates how the tTRBD establishes the template boundary by positioning the TBE at the correct distance from the TERT active site to prohibit copying of nontemplate nucleotides
Conceptual mechanization studies for a horizon definition spacecraft structures and thermal subsystem
Conceptual mechanization for horizon definition spacecraft structures and thermal subsystem - spin-stabilized, hexagonal cylinder for launch of two-stage Improved Delta /DSV-3N
Performance optimization and load-balancing modeling for superparametrization by 3D LES
In order to eliminate climate uncertainty w.r.t. cloud and convection parametrizations, superpramaterization (SP) [1] has emerged as one of the possible ways forward. We have implemented (regional) superparametrization of the ECMWF weather model OpenIFS [2] by cloud-resolving, three-dimensional large-eddy simulations. This setup, described in [3], contains a two-way coupling between a global meteorological model that resolves large-scale dynamics, with many local instances of the Dutch Atmospheric Large Eddy Simulation (DALES) [4], resolving cloud and boundary layer physics. The model is currently prohibitively expensive to run over climate or even seasonal time scales, and a global SP requires the allocation of millions of cores. In this paper, we study the performance and scaling behavior of the LES models and the coupling code and present our implemented optimizations. We mimic the observed load imbalance with a simple performance model and present strategies to improve hardware utilization in order to assess the feasibility of a world-covering superparametrization. We conclude that (quasi-)dynamical load-balancing can significantly reduce the runtime for such large-scale systems with wide variability in LES time-stepping speeds
A critical analysis of the hydrino model
Recently, spectroscopic and calorimetric observations of hydrogen plasmas and
chemical reactions with them have been interpreted as evidence for the
existence of electronic states of the hydrogen atom with a binding energy of
more than 13.6 eV. The theoretical basis for such states, that have been dubbed
hydrinos, is investigated. We discuss both, the novel deterministic model of
the hydrogen atom, in which the existence of hydrinos was predicted, and
standard quantum mechanics. Severe inconsistencies in the deterministic model
are pointed out and the incompatibility of hydrino states with quantum
mechanics is reviewed.Comment: 9 page
Microbiome Aggregated Traits and Assembly are More Sensitive to Soil Management than Diversity
How soil is managed, particularly for agriculture, exerts stresses upon soil microbiomes resulting in altered community structures and functional states. Understanding how soil microbiomes respond to combined stresses is important for predicting system performance under different land use scenarios, aids in identification of the most environmentally benign managements and provides insight into how system function can be recovered in degraded soils. We use a long-established field experiment to study the effects of combined chronic disturbance of the magnitude of organic carbon inputs with acute effects of physical disturbance by tillage. We show that because of the variety of ways it can be assessed, biodiversity – here based on microbial small subunit ribosomal RNA genes – does not provide a consistent view of community change. In contrast, aggregated traits associated with soil microbiomes indicate general loss of function, measured as a reduction of average genome lengths, associated with chronic reduction of organic inputs in arable or bare fallow soils and altered growth strategies associated with ribosomal RNA operon copy number in prokaryotes, as well as a switch to pathogenicity in fungal communities. In addition, pulse disturbance by soil tillage is associated with an increased influence of stochastic processes upon prokaryote community assembly, but fungicide used in arable soils results in niche assembly of fungal communities compared to untilled grassland. Overall, bacteria, archaea and fungi do not share a common response to land management change and estimates of biodiversity do not capture important facets of community adaptation to stresses adequately
High-accuracy sampling of saproxylic diversity indicators at regional scales with pheromones: The case of "Elater ferrugineus" (Coleoptera, Elateridae)
The rare beetle Elater ferrugineus was sampled at 47 sites in the county of Östergötland, Sweden by means of pheromone-baited traps to assess its value as an indicator species for hollow oak stands rich in rare saproxylic beetle species. In addition, Osmoderma eremita was also sampled with pheromone baits. These data were then compared against species survey data collected at the same sites by pitfall and window traps. Both species co-occur with many Red Listed saproxylic beetles, with E. ferrugineus being a somewhat better indicator for the rarest species. The conservation value of a site (measured as Red List points or number of Red Listed species) increased with the number of specimens of E. ferrugineus and O. eremita caught. Accuracy of sampling by means of pheromone trapping turned out to be radically different for the two model species. E. ferrugineus traps put out during July obtained full accuracy after only 6 days, whereas O. eremita traps needed to be out from early July to mid-August in order to obtain full accuracy with one trap per site. By using E. ferrugineus, or preferably both species, as indicator species, accuracy would increase and costs decrease for saproxylic biodiversity sampling, monitoring and identification of hotspots
Electronic structure investigation of Ti3AlC2, Ti3SiC2, and Ti3GeC2 by soft-X-ray emission spectroscopy
The electronic structures of epitaxially grown films of Ti3AlC2, Ti3SiC2 and
Ti3GeC2 have been investigated by bulk-sensitive soft X-ray emission
spectroscopy. The measured high-resolution Ti L, C K, Al L, Si L and Ge M
emission spectra are compared with ab initio density-functional theory
including core-to-valence dipole matrix elements. A qualitative agreement
between experiment and theory is obtained. A weak covalent Ti-Al bond is
manifested by a pronounced shoulder in the Ti L-emission of Ti3AlC2. As Al is
replaced with Si or Ge, the shoulder disappears. For the buried Al and
Si-layers, strongly hybridized spectral shapes are detected in Ti3AlC2 and
Ti3SiC2, respectively. As a result of relaxation of the crystal structure and
the increased charge-transfer from Ti to C, the Ti-C bonding is strengthened.
The differences between the electronic structures are discussed in relation to
the bonding in the nanolaminates and the corresponding change of materials
properties.Comment: 15 pages, 8 figure
Speech disfluencies in typically developing Finnish-speaking children - preliminary results
We investigated the speech disfluencies of 54 typically fluent Finnish-speaking children: 14 children randomly selected from a longitudinal study (age levels 2, 3, and 4 years), and 40 children from a cross-sectional study (age levels 6, 7, 8, and 9 years). Speech samples, collected during a semi-structured conversation, were analysed for disfluencies per 100 words and 100 syllables. No significant within-age effect was found for the total frequency of disfluencies or disfluency types among the 2- to 4-year-olds. Across the 6- to 9-year-olds, between-group differences were found for the total frequency and type of disfluencies. Clinically relevant was that the criterion to distinguish normally fluent children from those who stutter, i.e., <3 stuttering-like disfluencies (SLD) per 100 syllables, was applicable in all age groups whereas the criterion <3SLD per 100 words was not. Consequently, these preliminary results suggest that different guidelines are needed for defining normal disfluency from stuttering in different languages
Hopping conduction in strong electric fields: Negative differential conductivity
Effects of strong electric fields on hopping conductivity are studied
theoretically. Monte-Carlo computer simulations show that the analytical theory
of Nguyen and Shklovskii [Solid State Commun. 38, 99 (1981)] provides an
accurate description of hopping transport in the limit of very high electric
fields and low concentrations of charge carriers as compared to the
concentration of localization sites and also at the relative concentration of
carriers equal to 0.5. At intermediate concentrations of carriers between 0.1
and 0.5 computer simulations evidence essential deviations from the results of
the existing analytical theories.
The theory of Nguyen and Shklovskii also predicts a negative differential
hopping conductivity at high electric fields. Our numerical calculations
confirm this prediction qualitatively. However the field dependence of the
drift velocity of charge carriers obtained numerically differs essentially from
the one predicted so far. Analytical theory is further developed so that its
agreement with numerical results is essentially improved.Comment: 12 pages, 12 figures. References added, figures improve
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