5 research outputs found
Generation of Mutants from the 57B Region of Drosophila melanogaster
The 57B region of Drosophila melanogaster includes a cluster of the three homeobox genes
orthopedia (otp), Drosophila Retinal homeobox (DRx), and homeobrain (hbn). In an attempt to isolate mu tants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B
region, among them mutants for otp, DRx, and hbn. With the help of two newly generated deletions
from the 57B region, we mapped additional mutants to specific chromosomal intervals and identi fied several of these mutants from the 57B region molecularly. In addition, we generated mutants
for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650,
and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during develop ment for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B
region of Drosophila, which could now be used to further explore the functions of the corresponding
genes in the future