Structure of discontinuities in kinetoplast DNA-associated minicircles during S phase in Crithidia fasciculata

Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of thousands of interlocked minicircles and 20–30 maxicircles. The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized strands remain at the time of reattachment to the kDNA network. We show here that the steady-state population of replicated, network-associated minicircles only becomes repaired to the point of having nicks with a 3′OH and 5′deoxyribonucleoside monophosphate during S phase. These nicks represent the origin/terminus of the strand and occur within the replication origins (oriA and oriB) located 180° apart on the minicircle. Minicircles containing a new L strand have a single nick within either oriA or oriB but not in both origins in the same molecule. The discontinuously synthesized H strand contains single nicks within both oriA and oriB in the same molecule implying that discontinuities between the H-strand Okazaki fragments become repaired except for the fragments initiated within the two origins. Nicks in L and H strands at the origins persist throughout S phase and only become ligated as a prelude to network division. The failure to ligate these nicks until just prior to network division is not due to inappropriate termini for ligation

The Effectiveness of a Color-Coded, Onset-Rime Reading Intervention With First Grade Students At Serious Risk For Reading Disabilities

Beginning readers who are weak decoders usually continue to fall behind in reading as they progress through school, negatively affecting their overall academic performance, self-esteem, and motivation. Therefore, it is imperative to develop instructional practices to assist the acquisition of effective decoding skills. Although existing remedial approaches have proven effective with a number of students with reading problems, they have not been successful with those students most at risk and have generally not resulted in transfer of skills to decoding novel words not targeted in instruction. I used a single-subject multiple probe design across participants to investigate the effectiveness of a color-coded, onset-rime based decoding intervention. The participants were first grade students determined to be at serious risk for reading disabilities based on their performance on screening measures. All four of the students made strong progress in learning the instructional words, increasing on average 73% over baseline (range 66%-78%). In addition, for novel words from instructed rime patterns, students increased their scores from baseline to post-intervention by an average of 56% (range 50% to 62%). There was limited transfer at the vowel level to uninstructed rime patterns, with students improving their scores by an average of 29% (range 17% to 50%). All students maintained their improvement in decoding skills for both instructional and transfer words at one week and one month maintenance. The fact that the children were able not only to master instructional words but also to use their knowledge of rime patterns to decode uninstructed words is important given the difficulty of students most at-risk for reading disabilities to master instructional words and transfer decoding gains. Furthermore, the three participants with the lowest performance prior to instruction showed strong improvement on a standardized measure of reading achievement (Woodcock-Johnson Reading Mastery Test-Revised, Normative Update). The effectiveness of the program in improving the decoding skills of readers who are significantly at-risk is a promising first step in finding an instructional approach that is successful with students who have been left behind not just by traditional classroom instruction but by remedial approaches as well

Unobscured Type 2 Active Galactic Nuclei

Type 2 active galactic nuclei (AGNs) with intrinsically weak broad emission lines (BELs) would be exceptions to the unified model. After examining a number of proposed candidates critically, we find that the sample is contaminated significantly by objects with BELs of strengths indicating that they actually contain intermediate-type AGNs, plus a few Compton-thick sources as revealed by extremely low ratios of X-ray to nuclear IR luminosities. We develop quantitative metrics that show two (NGC 3147 and NGC 4594) of the remaining candidates to have BELs 2-3 orders of magnitude weaker than those of typical type 1 AGNs. Several more galaxies remain as candidates to have anomalously weak BELs, but this status cannot be confirmed with the existing information. Although the parent sample is poorly defined, the two confirmed objects are well under 1% of its total number of members, showing that the absence of a BEL is possible, but very uncommon in AGN. We evaluate these two objects in detail using multi-wavelength measurements including new IR data obtained with Spitzer and ground-based optical spectropolarimeteric observations. They have little X-ray extinction with N_H < ~10^(21) cm^(–2). Their IR spectra show strong silicate emission (NGC 4594) or weak aromatic features on a generally power-law continuum with a suggestion of silicates in emission (NGC 3147). No polarized BEL is detected in NGC 3147. These results indicate that the two unobscured type 2 objects have circumnuclear tori that are approximately face-on. Combined with their X-ray and optical/UV properties, this behavior implies that we have an unobscured view of the nuclei and thus that they have intrinsically weak BELs. We compare their properties with those of the other less-extreme candidates. We then compare the distributions of bolometric luminosities and accretion rates of these objects with theoretical models that predict weak BELs

An Updated Algorithm for Estimation of Pesticide Exposure Intensity in the Agricultural Health Study

An algorithm developed to estimate pesticide exposure intensity for use in epidemiologic analyses was revised based on data from two exposure monitoring studies. In the first study, we estimated relative exposure intensity based on the results of measurements taken during the application of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) (n = 88) and the insecticide chlorpyrifos (n = 17). Modifications to the algorithm weighting factors were based on geometric means (GM) of post-application urine concentrations for applicators grouped by application method and use of chemically-resistant (CR) gloves. Measurement data from a second study were also used to evaluate relative exposure levels associated with airblast as compared to hand spray application methods. Algorithm modifications included an increase in the exposure reduction factor for use of CR gloves from 40% to 60%, an increase in the application method weight for boom spray relative to in-furrow and for air blast relative to hand spray, and a decrease in the weight for mixing relative to the new weights assigned for application methods. The weighting factors for the revised algorithm now incorporate exposure measurements taken on Agricultural Health Study (AHS) participants for the application methods and personal protective equipment (PPE) commonly reported by study participants

Atrazine and cancer incidence among pesticide applicators in the agricultural health study (1994-2007).

Atrazine is a triazine herbicide used widely in the United States. Although it is an animal carcinogen, the mechanism in rodents does not appear to operate in humans. Few epidemiologic studies have provided evidence for an association

Unobscured Type 2 AGNs

Type 2 AGNs with intrinsically weak broad emission lines (BELs) would be exceptions to the unified model. After examining a number of proposed candidates critically, we find that the sample is contaminated significantly by objects with BELs of strengths indicating that they actually contain intermediate-type AGNs, plus a few Compton-thick sources as revealed by extremely low ratios of X-ray to nuclear IR luminosities. We develop quantitative metrics that show two (NGC 3147 and NGC 4594) of the remaining candidates to have BELs 2-3 orders of magnitude weaker than those of typical type-1 AGNs. Several more galaxies remain as candidates to have anomalously weak BELs, but this status cannot be confirmed with the existing information. Although the parent sample is poorly defined, the two confirmed objects are well under 1% of its total number of members, showing that the absence of a BEL is possible, but very uncommon in AGN. We evaluate these two objects in detail using multi-wavelength measurements. They have little X-ray extinction with N_H < 10^21 cm^{-2}. Their IR spectra show strong silicate emission (NGC 4594) or weak aromatic features on a generally power law continuum with a suggestion of silicates in emission (NGC 3147). No polarized BEL is detected in NGC 3147. These results indicate that the two unobscured type-2 objects have circumnuclear tori that are approximately face-on. Combined with their X-ray and optical/UV properties, this behavior implies that we have an unobscured view of the nuclei and thus that they have intrinsically weak BELs. We compare their properties with those of the other less-extreme candidates. We then compare the distributions of bolometric luminosities and accretion rates of these objects with theoretical models that predict weak BELs.Comment: Accepted for publication in ApJ, 17 pages, 13 figure

24 MICRON PROPERTIES OF X-RAY-SELECTED ACTIVE GALACTIC NUCLEI

We examine the 24 μm to X-ray color of 157 X-ray-selected active galactic nuclei (AGNs) as a function of X-ray obscuration and optical classification in the Chandra Deep Field-South. The sample consists of the Chandra hard-band detections with 2-8 keV flux above 10-15 ergs s-1 cm-2. A deep 24 μm mosaic obtained with Spitzer provides mid-infrared fluxes for the sample. Since obscured AGNs locally have higher 24 μm/2-8 keV flux ratios than unobscured AGNs, and since X-ray background models predict a large population of obscured AGNs, we expect to find many X-ray-hard, IR-bright AGNs. Instead, we find that the 24 μm to X-ray flux ratio does not depend on X-ray hardness in the full sample, nor does it differ between narrow- and broad-line AGNs. We identify five nearly Compton-thick AGNs and find they have similar 24 μm to X-ray flux ratios compared to the full sample. We consider AGNs in the narrow redshift spikes at z ~ 0.7; for these AGNs, there is some evidence that the flux ratio increases with X-ray hardness. The redshift slice also shows an odd trend that is also prominent in the full sample: a group of X-ray-hard AGNs with very low 24 μm to X-ray flux ratios

A Spectropolarimetric Test of the Structure of the Intrinsic Absorbers in the Quasar HS1603+3820

We report the results of a spectropolarimetric observation of the C IV mini-BAL in the quasar HS1603+3820. The observations were carried out with the FOCAS instrument on the Subaru telescope and yielded an extremely high polarization sensitivity of 0.1%, at a resolving power of 1500. HS1603+3820 has been the target of a high-resolution spectroscopic monitoring campaign for more than four years, aimed at studying its highly variable C IV mini-BAL profile. Using the monitoring observations, in an earlier paper we were able to narrow down the causes of the variability to the following two scenarios: (1) scattering material of variable optical depth redirecting photons around the absorber, and (2) a variable, highly-ionized screen between the continuum source and the absorber which modulates the UV continuum incident on the absorber. The observations presented here provide a crucial test of the scattering scenario and lead us to disfavor it because (a) the polarization level is very small (p~0.6%) throughout the spectrum, and (b) the polarization level does not increase across the mini-BAL trough. Thus, the variable screen scenario emerges as our favored explanation of the C IV mini-BAL variability. Our conclusion is bolstered by recent X-ray observations of nearby mini-BAL quasars, which show a rapidly variable soft X-ray continuum that appears to be the result of transmission through an ionized absorber of variable ionization parameter and optical depth.Comment: To appear in the Astrophysical Journal, vol. 719, August 201

Absolute Calibration and Characterization of the Multiband Imaging Photometer for Spitzer. II. 70 micron Imaging

The absolute calibration and characterization of the Multiband Imaging Photometer for Spitzer (MIPS) 70 micron coarse- and fine-scale imaging modes are presented based on over 2.5 years of observations. Accurate photometry (especially for faint sources) requires two simple processing steps beyond the standard data reduction to remove long-term detector transients. Point spread function (PSF) fitting photometry is found to give more accurate flux densities than aperture photometry. Based on the PSF fitting photometry, the calibration factor shows no strong trend with flux density, background, spectral type, exposure time, or time since anneals. The coarse-scale calibration sample includes observations of stars with flux densities from 22 mJy to 17 Jy, on backgrounds from 4 to 26 MJy sr^-1, and with spectral types from B to M. The coarse-scale calibration is 702 +/- 35 MJy sr^-1 MIPS70^-1 (5% uncertainty) and is based on measurements of 66 stars. The instrumental units of the MIPS 70 micron coarse- and fine-scale imaging modes are called MIPS70 and MIPS70F, respectively. The photometric repeatability is calculated to be 4.5% from two stars measured during every MIPS campaign and includes variations on all time scales probed. The preliminary fine-scale calibration factor is 2894 +/- 294 MJy sr^-1 MIPS70F^-1 (10% uncertainty) based on 10 stars. The uncertainty in the coarse- and fine-scale calibration factors are dominated by the 4.5% photometric repeatability and the small sample size, respectively. The 5-sigma, 500 s sensitivity of the coarse-scale observations is 6-8 mJy. This work shows that the MIPS 70 micron array produces accurate, well calibrated photometry and validates the MIPS 70 micron operating strategy, especially the use of frequent stimulator flashes to track the changing responsivities of the Ge:Ga detectors.Comment: 19 pages, PASP, in pres