Silver nanoparticle-doped zirconia capillaries for enhanced bacterial filtration

Membrane clogging and biofilm formation are the most serious problems during water filtration. Silver nanoparticle (Agnano) coatings on filtration membranes can prevent bacterial adhesion and the initiation of biofilm formation. In this study, Agnano are immobilized via direct reduction on porous zirconia capillary membranes to generate a nanocomposite material combining the advantages of ceramics being chemically, thermally and mechanically stable with nanosilver, an efficient broadband bactericide for water decontamination. The filtration of bacterial suspensions of the fecal contaminant Escherichia coli reveals highly efficient bacterial retention capacities of the capillaries of 8 log reduction values, fulfilling the requirements on safe drinking water according to the U.S. Environmental Protection Agency. Maximum bacterial loading capacities of the capillary membranes are determined to be 3 × 109 bacterial cells/750 mm2 capillary surface until back flushing is recommendable. The immobilized Agnano remain accessible and exhibit strong bactericidal properties by killing retained bacteria up to maximum bacterial loads of 6 × 108 bacterial cells/750 mm2 capillary surface and the regenerated membranes regain filtration efficiencies of 95–100%. Silver release is moderate as only 0.8% of the initial silver loading is leached during a three-day filtration experiment leading to average silver contaminant levels of 100 μg/L

TuLUMIS - a tunable LED-based underwater multispectral imaging system

Multispectral imaging (MSI) is widely used in terrestrial applications to help increase the discriminability between objects of interest. While MSI has shown potential for underwater geological and biological surveys, it is thus far rarely applied underwater. This is primarily due to the fact light propagation in water is subject to wavelength dependent attenuation and tough working conditions in the deep ocean. In this paper, a novel underwater MSI system based on a tunable light source is presented which employs a monochrome still image camera with flashing, pressure neutral color LEDs. Laboratory experiments and field tests were performed. Results from the lab experiments show an improvement of 76.66% on discriminating colors on a checkerboard by using the proposed imaging system over the use of an RGB camera. The field tests provided in situ MSI observations of pelagic fauna, and showed the first evidence that the system is capable of acquiring useful imagery under real marine conditions

Numerical Simulations and Experiments of Ignition of Solid Particles in a Laminar Burner:Effects of Slip Velocity and Particle Swelling

Ignition and combustion of pulverized solid fuel is investigated in a laminar burner. The two-dimensional OH radical field is measured in the experiments, providing information on the first onset of ignition and a detailed characterization of the flame structure for the single particle. In addition, particle velocity and diameter are tracked in time in the experiments. Simulations are carried out with a Lagrangian point-particle approach fully coupled with an Eulerian solver for the gas-phase, which includes detailed chemistry and transport. The numerical simulation results are compared with the experimental measurements in order to investigate the ignition characteristics. The effect of the slip velocity, i.e. the initial velocity difference between the gas-phase and the particle, is investigated numerically. For increasing slip velocity, the ignition delay time decreases. For large slip velocities, the decrease in ignition delay time is found to saturate to a value which is about 40% smaller than the ignition delay time at zero slip velocity. Performing a simulation neglecting the dependency of the Nusselt number on the slip velocity, it is found that this dependency does not play a role. On the contrary, it is found that the decrease of ignition delay time induced by the slip velocity is due to modifications of the temperature field around the particle. In particular, the low-temperature fluid related to the energy sink due to particle heating is transported away from the particle position when the slip velocity is non-zero; therefore, the particle is exposed to larger temperatures. Finally, the effect of particle swell is investigated using a model for the particle swelling based on the CPD framework. With this model, we observed negligible differences in ignition delay time compared to the case in which swelling is not included. This is related to the negligible swelling predicted by this model before ignition. However, this is inconsistent with the experimental measurements of particle diameter, showing a significant increase of diameter even before ignition. In further simulations, the measured swelling was directly prescribed, using an analytical fit at the given conditions. With this approach, it is found that the inclusion of swelling reduces the ignition delay time by about 20% for small particles while it is negligible for large particles

Homogeneous ignition and volatile combustion of single solid fuel particles in air and oxy-fuel conditions

The ignition and volatile combustion of single coal particles were investigated under laminar conditions. Relevant physico-chemical processes were analyzed under conventional and oxy-fuel atmospheres with varying O2 contents in experiments and simulations. An optically accessible laminar flow reactor with well-defined boundary conditions measured with PIV and quantitative OH-LIF was employed. Multi-parameter optical diagnostics were conducted, including OH-LIF, luminescence imaging, and backlight illumination. Simultaneously acquired experimental data allowed for the evaluation of particle size, ignition delay time, and volatile combustion duration for individual particles. A statistical analysis revealed the improved accuracy of OH-LIF compared to luminescence imaging regarding ignition detection. Simulations within an Eulerian-Lagrangian framework were introduced and validated against experiments. On this basis, particle temperatures, local gas temperatures, and fuel mass fraction were evaluated, providing insights into the devolatilization. Both experimental and numerical results indicated that increasing particle sizes significantly retarded homogeneous ignition and volatile consumption. When increasing the O2 content, a shorter ignition delay time and volatile combustion duration were observed experimentally, which was more significant for larger particles. High slip velocities accelerated convective transport resulting in an earlier ignition and faster volatile combustion. An atmosphere change from N2 to CO2 showed an earlier ignition and increased volatile combustion duration for larger particles, whereas the differences were insignificant for small particles. Simulation results suggested that the local heat transfer was improved by CO2, mainly due to the lower temperature sink close to particles and hence higher volatile release rates. As the initial ambient temperatures were similar, the introduction of CO2 favored homogeneous ignition and slowed down the volatile consumption

DeepSurveyCam — A Deep Ocean Optical Mapping System

Underwater photogrammetry and in particular systematic visual surveys of the deep sea are by far less developed than similar techniques on land or in space. The main challenges are the rough conditions with extremely high pressure, the accessibility of target areas (container and ship deployment of robust sensors, then diving for hours to the ocean floor), and the limitations of localization technologies (no GPS). The absence of natural light complicates energy budget considerations for deep diving flash-equipped drones. Refraction effects influence geometric image formation considerations with respect to field of view and focus, while attenuation and scattering degrade the radiometric image quality and limit the effective visibility. As an improvement on the stated issues, we present an AUV-based optical system intended for autonomous visual mapping of large areas of the seafloor (square kilometers) in up to 6000 m water depth. We compare it to existing systems and discuss tradeoffs such as resolution vs. mapped area and show results from a recent deployment with 90,000 mapped square meters of deep ocean floor

Making marine image data FAIR

Underwater images are used to explore and monitor ocean habitats, generating huge datasets with unusual data characteristics that preclude traditional data management strategies. Due to the lack of universally adopted data standards, image data collected from the marine environment are increasing in heterogeneity, preventing objective comparison. The extraction of actionable information thus remains challenging, particularly for researchers not directly involved with the image data collection. Standardized formats and procedures are needed to enable sustainable image analysis and processing tools, as are solutions for image publication in long-term repositories to ascertain reuse of data. The FAIR principles (Findable, Accessible, Interoperable, Reusable) provide a framework for such data management goals. We propose the use of image FAIR Digital Objects (iFDOs) and present an infrastructure environment to create and exploit such FAIR digital objects. We show how these iFDOs can be created, validated, managed and stored, and which data associated with imagery should be curated. The goal is to reduce image management overheads while simultaneously creating visibility for image acquisition and publication efforts

What Is Resistance? Impact of Phenotypic versus Molecular Drug Resistance Testing on Therapy for Multi- and Extensively Drug-Resistant Tuberculosis.

Rapid and accurate drug susceptibility testing (DST) is essential for the treatment of multi- and extensively drug-resistant tuberculosis (M/XDR-TB). We compared the utility of genotypic DST assays with phenotypic DST (pDST) using Bactec 960 MGIT or Löwenstein-Jensen to construct M/XDR-TB treatment regimens for a cohort of 25 consecutive M/XDR-TB patients and 15 possible anti-TB drugs. Genotypic DST results from Cepheid GeneXpert MTB/RIF (Xpert) and line probe assays (LPAs; Hain GenoType MTBDRplus 2.0 and MTBDRsl 2.0) and whole-genome sequencing (WGS) were translated into individual algorithm-derived treatment regimens for each patient. We further analyzed if discrepancies between the various methods were due to flaws in the genotypic or phenotypic test using MIC results. Compared with pDST, the average agreement in the number of drugs prescribed in genotypic regimens ranged from just 49% (95% confidence interval [CI], 39 to 59%) for Xpert and 63% (95% CI, 56 to 70%) for LPAs to 93% (95% CI, 88 to 98%) for WGS. Only the WGS regimens did not contain any drugs to which pDST showed resistance. Importantly, MIC testing revealed that pDST likely underestimated the true rate of resistance for key drugs (rifampin, levofloxacin, moxifloxacin, and kanamycin) because critical concentrations (CCs) were too high. WGS can be used to rule in resistance even in M/XDR strains with complex resistance patterns, but pDST for some drugs is still needed to confirm susceptibility and construct the final regimens. Some CCs for pDST need to be reexamined to avoid systematic false-susceptible results in low-level resistant isolates

Updating the approaches to define susceptibility and resistance to anti-tuberculosis agents: implications for diagnosis and treatment

11 páginas, 2 figuras, 1 tablaInappropriately high breakpoints have resulted in systematic false-susceptible AST results to anti-TB drugs. MIC, PK/PD and clinical outcome data should be combined when setting breakpoints to minimise the emergence and spread of antimicrobial resistance.I. Comas was supported by PID2019-104477RB-I00 from the Spanish Science Ministry and by ERC (CoG 101001038)Peer reviewe

Lichtstreuung in trüben Flüssigkeiten

This work is about evaluation of light scattering experiments. The static and dynamic scattering properties of polystyrene-latex samples and of a critical sample of the system 3-methyl-pyridine/water/NaBr were investigated. The scattering experiments were performed using a 3D-cross-correlation setup.Samples of high to low transmissions were investigated. In highly turbid samples multiply scattered light contributes to the scattering intensities and correlation functions. An evaluation using traditional light scattering theory, valid for transparent samples and single scattering, leads to erroneous results. Therefore it was reasonable to precisely estimate the contribution of multiply scattered light to the scattering intensities and the correlation functions.The first part of this work describes light scattering experiments of latex samples. The detector deficiencies were determined by evaluating the dependence of the amplitudes of the auto correlation functions of the polarized scattered light on the countrates. Thus it was possible to convert the experimental countrates and correlation functions into their ideal counterparts. The contributions of single and multiple scattering to the scattering intensities were calculated from the amplitudes of the 3D-cross-correlation functions. The field correlation functions of the single and of the multiple scattering were determined from the ideal auto correlation functions and the 3D-cross-correlation functions. Monte-Carlo simulations were also applied to estimate the multiple scattering contributions. The simulation results were in good agreement with the experimental data. The influence of the turbidity on the angular dependency of the single scattering volume was analysed and correction functions were presented.The second part of this work deals with Monte-Carlo simulations of the static and dynamic light scattering of the critical sample. These simulations were also in good agreement with the experimental results

Lightscattering in Turbid Fluids

This work is about evaluation of light scattering experiments. The static and dynamic scattering properties of polystyrene-latex samples and of a critical sample of the system 3-methyl-pyridine/water/NaBr were investigated. The scattering experiments were performed using a 3D-cross-correlation setup.Samples of high to low transmissions were investigated. In highly turbid samples multiply scattered light contributes to the scattering intensities and correlation functions. An evaluation using traditional light scattering theory, valid for transparent samples and single scattering, leads to erroneous results. Therefore it was reasonable to precisely estimate the contribution of multiply scattered light to the scattering intensities and the correlation functions.The first part of this work describes light scattering experiments of latex samples. The detector deficiencies were determined by evaluating the dependence of the amplitudes of the auto correlation functions of the polarized scattered light on the countrates. Thus it was possible to convert the experimental countrates and correlation functions into their ideal counterparts. The contributions of single and multiple scattering to the scattering intensities were calculated from the amplitudes of the 3D-cross-correlation functions. The field correlation functions of the single and of the multiple scattering were determined from the ideal auto correlation functions and the 3D-cross-correlation functions. Monte-Carlo simulations were also applied to estimate the multiple scattering contributions. The simulation results were in good agreement with the experimental data. The influence of the turbidity on the angular dependency of the single scattering volume was analysed and correction functions were presented.The second part of this work deals with Monte-Carlo simulations of the static and dynamic light scattering of the critical sample. These simulations were also in good agreement with the experimental results