Impact of Brain Breaks on Student Engagement in an Upper Elementary Classroom

Educators are constantly looking for ways to increase student engagement. Due to limited attention spans in students and the academic stress that is placed on them, students often become disengaged and distracted after extended periods of academic related tasks. Brain breaks, or short periods of movement or relaxation to break up academic tasks, can improve student engagement and focus. One specific benefit was improved focus, which has a positive impact on time-on-task behavior (Cline, et al., 2021). This study will concentrate on three different types of brain breaks; physical brain breaks that involve student movement, mindfulness brain breaks that involve breathing and relaxation, and a combination brain break where a quick movement break is followed by a breathing component. Following each brain break, the researchers will track the number of redirections required to bring focus back to the academic task. Students will also complete a reflection for the researchers to identify if students enjoyed each type of break and if they felt it helped them refocus. It will be explored if there is a difference in student engagement following each type of break and if a specific type of break is more highly enjoyed by the students. The goal is to find a classification of breaks that are both effective and enjoyable for the students

Presynchronizing PGF2α and GnRH injections before timed artificial insemination CO-Synch + CIDR program

Fixed-time artificial insemination is an effective management tool that reduces the labor associated with more conventional artificial insemination programs requiring detection of estrus. The 7-day CO-Synch + controlled internal drug release (CIDR) insert protocol has been shown to effectively initiate estrus and ovulation in cycling and non-cycling suckled beef cows, producing pregnancy rates at or greater than 50% in beef cows. The gonadotropin-releasing hormone (GnRH) injection that begins the CO-Synch + CIDR program initiates ovulation in a large proportion of cows, particularly anestrous cows. The CIDR, which releases progesterone intravaginally, prevents short estrous cycles that usually follow the first postpartum ovulation in beef cows. Our hypothesis was that inducing estrus with a prostaglandin injection followed 3 days later with a GnRH injection, 7 days before applying the 7-day CO-Synch + CIDR protocol, might increase the percentage of cycling cows that would exhibit synchronous follicular waves after the onset of the CO-Synch + CIDR protocol. We also hypothesized that the additional GnRH injection would increase the percentage of anestrous cows that would ovulate, thereby increasing pregnancy outcomes

Extending observations further: using historic biogeochemical data to understand trends in Puget Sound

Fluctuations in Puget Sound water quality reflect a combination of natural variability and anthropogenic influence. Predictions of how future changes will unfold requires an understanding of the complex interplay between these factors. Recent and ongoing measurement of water quality parameters allow a thorough assessment of variability over short time scales, but understanding changes over longer (e.g., decadal) timescales is critical for forecasting future changes. Examining long term trends requires the use of historical data, however, measurements that pre-date modern monitoring programs can be scarce, and the detection limits are often higher than newer analytical techniques. Here, we will consider several possible methods to stitch together modern and historical data records of dissolved oxygen and nutrient concentrations while accounting for differences in sample rate, temporal coverage, and sensitivity of analytical techniques. To test different data analysis methods, data records with good temporal coverage that show discernible trends and/or high covariance with other environmental parameters will be used as the basis for simulating data with spotty temporal coverage (as might be expected for less commonly measured biogeochemical parameters like deep dissolved oxygen). Selected trend analyses will then be applied to these simulated time series records to assess method effectiveness in identifying a known feature. Top performing methods for trend analysis will then be applied to key biogeochemical parameters such as nitrate and deep dissolved oxygen. We report on the major covariates and trends identified and the associated statistical confidence intervals. These results will be part of a larger effort to determine how existing trends might play out under different anthropogenic input scenarios

Effects of Corn Processing Method and Crude Protein Level with the Inclusion of Wet Corn Gluten Feed on Finishing Steer Performance

Three hundred twenty crossbred steer calves were used to evaluate corn processing method and crude protein level in finishing diets that included wet corn gluten feed. There was no response due to crude protein level (14 vs 15%) observed in this trial. As corn processing method became more intensive (fine-grinding, high-moisture ensiling, and steam-flaking corn) compared to dry-rolling, daily intake was reduced. Daily gain was similar across corn processing methods. Feed efficiency and cost of gain improved as corn processing method intensity increased

Nutrient dynamics and ties to environmental conditions and drivers in central Puget Sound

Real-time and near-continuous in-situ measurements can provide new insights into variability and patterns of a marine system that would otherwise be undetected by sampling at a lower temporal resolution. King County has a comprehensive long-term marine water quality monitoring program in the Central Basin of Puget Sound that currently includes: bi-monthly water column vertical profiles, discrete sampling, near-surface real-time moored sensors, and new quantitative phytoplankton and zooplankton components. These data can assist with identifying changes and linkages in biogeochemical and ecological processes as well as changes in climate patterns. Recently, new moored and profiling optical nitrate sensors were added to existing monitoring systems to better understand nutrient dynamics. The mooring provides data at 15-minute intervals and the profiling sensor provides data throughout the water column at multiple sites. Nitrate data from 2016-17 are explored and tied to diurnal and seasonal patterns in physical conditions and phytoplankton dynamics. Our data show that variations in nitrate are tightly coupled to in-situ chlorophyll observations. During the phytoplankton growing season, near-surface nitrate may vary up to one order of magnitude in a short time period, such as from 0.03 to 0.32 mg/L in a day. Interannual differences are largely influenced by changes in river flow and oceanic inputs, weather, and climate. These data are framed in historical context of the long-term status and trends in nutrients from discrete sampling over the past two decades. By increasing our understanding of changes and drivers in nutrient patterns, these types of data records can help to inform models and management decisions, and better apply effective remediation targets for Puget Sound marine water quality

A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis.

Temperature controls plant growth and development, and climate change has already altered the phenology of wild plants and crops1. However, the mechanisms by which plants sense temperature are not well understood. The evening complex is a major signalling hub and a core component of the plant circadian clock2,3. The evening complex acts as a temperature-responsive transcriptional repressor, providing rhythmicity and temperature responsiveness to growth through unknown mechanisms2,4-6. The evening complex consists of EARLY FLOWERING 3 (ELF3)4,7, a large scaffold protein and key component of temperature sensing; ELF4, a small α-helical protein; and LUX ARRYTHMO (LUX), a DNA-binding protein required to recruit the evening complex to transcriptional targets. ELF3 contains a polyglutamine (polyQ) repeat8-10, embedded within a predicted prion domain (PrD). Here we find that the length of the polyQ repeat correlates with thermal responsiveness. We show that ELF3 proteins in plants from hotter climates, with no detectable PrD, are active at high temperatures, and lack thermal responsiveness. The temperature sensitivity of ELF3 is also modulated by the levels of ELF4, indicating that ELF4 can stabilize the function of ELF3. In both Arabidopsis and a heterologous system, ELF3 fused with green fluorescent protein forms speckles within minutes in response to higher temperatures, in a PrD-dependent manner. A purified fragment encompassing the ELF3 PrD reversibly forms liquid droplets in response to increasing temperatures in vitro, indicating that these properties reflect a direct biophysical response conferred by the PrD. The ability of temperature to rapidly shift ELF3 between active and inactive states via phase transition represents a previously unknown thermosensory mechanism

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates the destabilization of key regulators of cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis that DET1 is essential for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of a deubiquitination module (DUBm). In contrast with yeast and metazoan DUB modules that are associated with the large SAGA complex, the Arabidopsis DUBm only comprises three proteins (hereafter named SGF11, ENY2 and UBP22) and appears to act independently as a major H2Bub deubiquitinase activity. Our study further unveils that DET1-DDB1-Associated-1 (DDA1) protein interacts with SGF11 in vivo, linking the DET1 complex to light-dependent ubiquitin-mediated proteolytic degradation of the DUBm. Collectively, these findings uncover a signaling path controlling DUBm availability, potentially adjusting H2Bub turnover capacity to the cell transcriptional status

Stage-specific requirement for Eomes in mature NK cell homeostasis and cytotoxicity

Natural killer (NK) cells are cytotoxic innate lymphoid cells (ILCs) that mediate antiviral and antitumor responses and require the transcriptional regulator Eomesodermin (Eomes) for early development. However, the role of Eomes and its molecular program in mature NK cell biology is unclear. To address this, we develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice. Eomes deletion after normal NK cell ontogeny results in a rapid loss of NK cells (but not ILC1s), with a particularly profound effect on penultimately mature stage III NK cells. Mechanisms responsible for stage III reduction include increased apoptosis and impaired maturation from stage II precursors. Induced Eomes deletion also decreases NK cell cytotoxicity and abrogates in vivo rejection of major histocompatibility complex (MHC)-class-I-deficient cells. However, other NK cell functional responses, and stage IV NK cells, are largely preserved. These data indicate that mature NK cells have distinct Eomes-dependent and -independent stages

A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii

In the unicellular green alga Chlamydomonas reinhardtii, the chloroplast-encoded tscA RNA is part of a tripartite group IIB intron, which is involved in trans-splicing of precursor mRNAs. We have used the yeast three-hybrid system to identify chloroplast group II intron RNA-binding proteins, capable of interacting with the tscA RNA. Of 14 candidate cDNAs, 13 encode identical polypeptides with significant homology to members of the nuclear nucleosome assembly protein (NAP) family. The RNA-binding property of the identified polypeptide was demonstrated by electrophoretic mobility shift assays using different domains of the tripartite group II intron as well as further chloroplast transcripts. Because of its binding to chloroplast RNA it was designated as NAP-like (cNAPL). In silico analysis revealed that the derived polypeptide carries a 46 amino acid chloroplast leader peptide, in contrast to nuclear NAPs. The chloroplast localization of cNAPL was demonstrated by laser scanning confocal fluorescence microscopy using different chimeric cGFP fusion proteins. Phylogenetic analysis shows that no homologues of cNAPL and its related nuclear counterparts are present in prokaryotic genomes. These data indicate that the chloroplast protein described here is a novel member of the NAP family and most probably has not been acquired from a prokaryotic endosymbiont

A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii

In the unicellular green alga Chlamydomonas reinhardtii, the chloroplast-encoded tscA RNA is part of a tripartite group IIB intron, which is involved in trans-splicing of precursor mRNAs. We have used the yeast three-hybrid system to identify chloroplast group II intron RNA-binding proteins, capable of interacting with the tscA RNA. Of 14 candidate cDNAs, 13 encode identical polypeptides with significant homology to members of the nuclear nucleosome assembly protein (NAP) family. The RNA-binding property of the identified polypeptide was demonstrated by electrophoretic mobility shift assays using different domains of the tripartite group II intron as well as further chloroplast transcripts. Because of its binding to chloroplast RNA it was designated as NAP-like (cNAPL). In silico analysis revealed that the derived polypeptide carries a 46 amino acid chloroplast leader peptide, in contrast to nuclear NAPs. The chloroplast localization of cNAPL was demonstrated by laser scanning confocal fluorescence microscopy using different chimeric cGFP fusion proteins. Phylogenetic analysis shows that no homologues of cNAPL and its related nuclear counterparts are present in prokaryotic genomes. These data indicate that the chloroplast protein described here is a novel member of the NAP family and most probably has not been acquired from a prokaryotic endosymbiont