498 research outputs found
Simultaneous growth of two cancer cell lines evidences variability in growth rates
Cancer cells co-cultured in vitro reveal unexpected differential growth rates
that classical exponential growth models cannot account for. Two
non-interacting cell lines were grown in the same culture, and counts of each
species were recorded at periodic times. The relative growth of population
ratios was found to depend on the initial proportion, in contradiction with the
traditional exponential growth model. The proposed explanation is the
variability of growth rates for clones inside the same cell line. This leads to
a log-quadratic growth model that provides both a theoretical explanation to
the phenomenon that was observed, and a better fit to our growth data
Towards real-time metabolic profiling of a biopsy specimen during a surgical operation by 1H high resolution magic angle spinning nuclear magnetic resonance: a case report
<p>Abstract</p> <p>Introduction</p> <p>Providing information on cancerous tissue samples during a surgical operation can help surgeons delineate the limits of a tumoral invasion more reliably. Here, we describe the use of metabolic profiling of a colon biopsy specimen by high resolution magic angle spinning nuclear magnetic resonance spectroscopy to evaluate tumoral invasion during a simulated surgical operation.</p> <p>Case presentation</p> <p>Biopsy specimens (n = 9) originating from the excised right colon of a 66-year-old Caucasian women with an adenocarcinoma were automatically analyzed using a previously built statistical model.</p> <p>Conclusions</p> <p>Metabolic profiling results were in full agreement with those of a histopathological analysis. The time-response of the technique is sufficiently fast for it to be used effectively during a real operation (17 min/sample). Metabolic profiling has the potential to become a method to rapidly characterize cancerous biopsies in the operation theater.</p
Draft Genome Sequence of the Flagellated Xanthomonas fuscans subsp. fuscans Strain CFBP 4884
Article de revue (Article scientifique dans une revue à comité de lecture)International audienceWe report the draft genome sequence of the flagellated strain CFBP 4884 of Xanthomonas fuscans subsp. fuscans, which was isolatedin an outbreak of common bacterial blight of beans along with non-flagellated strains. Comparative genomics will allowone to decipher the genomic diversity of strains cohabiting in epidemics.</p
Xanthomonas arboricola pv. juglandis and pv. corylina: Brothers or distant relatives? : genetic clues, epidemiology, and insights for disease management
Background: The species Xanthomonas arboricola comprises up to nine pathovars, two of which affect nut crops: pv. juglandis, the causal agent of walnut bacterial blight, brown apical necrosis, and the vertical oozing canker of Persian (English) walnut; and pv. corylina, the causal agent of the bacterial blight of hazelnut. Both pathovars share a complex population structure, represented by different clusters and several clades. Here we describe our current understanding of symptomatology, population dynamics, epidemiology, and disease control.
Taxonomic status: Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Lysobacterales (earlier synonym of Xanthomonadales); Family Lysobacteraceae (earlier synonym of Xanthomonadaceae); Genus Xanthomonas; Species X. arboricola; Pathovars: pv. juglandis and pv. corylina.
Host range and symptoms: The host range of each pathovar is not limited to a single species, but each infects mainly one plant species: Juglans regia (X. arboricola pv. juglandis) and Corylus avellana (X. arboricola. pv. corylina). Walnut bacterial blight is characterized by lesions on leaves and fruits, and cankers on twigs, branches, and trunks; brown apical necrosis symptoms consist of apical necrosis originating at the stigmatic end of the fruit. A peculiar symptom, the vertical oozing canker developing along the trunk, is elicited by a particular genetic lineage of the bacterium. Symptoms of hazelnut bacterial blight are visible on leaves and fruits as necrotic lesions, and on woody parts as cankers. A remarkable difference is that affected walnuts drop abundantly, whereas hazelnuts with symptoms do not.
Distribution: Bacterial blight of walnut has a worldwide distribution, wherever Persian (English) walnut is cultivated; the bacterial blight of hazelnut has a more limited distribution, although disease outbreaks are currently more frequently reported. X. arboricola pv. juglandis is regulated almost nowhere, whereas X. arboricola pv. corylina is regulated in most European and Mediterranean Plant Protection Organization (EPPO) countries.
Epidemiology and control: For both pathogens infected nursery material is the main pathway for their introduction and spread into newly cultivated areas; additionally, infected nursery material is the source of primary inoculum. X. arboricola pv. juglandis is also disseminated through pollen. Disease control is achieved through the phytosanitary certification of nursery material (hazelnut), although approved certification schemes are not currently available. Once the disease is present in walnut/hazelnut groves, copper compounds are widely used, mostly in association with dithiocarbamates; where allowed, antibiotics (preferably kasugamycin) are sprayed. The emergence of strains highly resistant to copper currently represents the major threat for effective management of the bacterial blight of walnut
Influence of pressing temperature on dynamics of strength of adhesive bond
ProuÄevali smo vpliv temperature stiskanja na utrjevanje urea-formaldehidnih (UF) lepil. Uporabili smo dve vrsti UF lepil proizvajalca Basf: Kaurit 345 z niĆŸjo vsebnostjo prostega formaldehida in Kaurit 350 z viĆĄjo vsebnostjo. PreizkuĆĄanci so bili standardni in sicer iz javorjevega furnirja, debeline 0,6 mm. PreizkuĆĄanci so se po razrezu klimatizirali pri sobnih pogojih: 23 °C in 55 % vlaĆŸnostjo. Kinetiko utrjevanja UF lepil smo izvedli z ABES (Automated Bonding Evaluation System) instrumentom. Lepilna meĆĄanica je bila skozi vse poizkuse konstantna, sestavljena iz 100 uteĆŸnih deleĆŸev vodne raztopine lepila in 1,5 % katalizatorja - amonijevega sulfata (glede na suho snov lepila). Testiranje je potekalo pri razliÄnih temperaturah stiskanja: 80, 100, 120, 150, 170 °C. Trajanje stiskanja smo prilagajali glede na razvoj striĆŸne trdnosti. Ko je ABES izmeril striĆŸno trdnost veÄjo od 0, smo meritev pri enakem Äasu stiskanja ponovili vsaj trikrat. Testirali smo tudi vpliv pH vrednosti razliÄnih furnirjev na utrjevanje UF lepil. Ker katalizator zniĆŸa pH vrednost meĆĄanice ter pospeĆĄi utrjevanje UF lepil, smo enak princip zniĆŸanja pH vrednosti ugotavljali s pomoÄjo razliÄnih lesnih vrst. Vsaka lesna vrsta ima razliÄno pH vrednost, ki tako dodatno pripomore k spremembi kislosti oz. baziÄnosti lepilne meĆĄanice med stiskanjem. Testirali smo preizkuĆĄance ĆĄestih razliÄnih drevesnih vrst (javor, bukev, hrast, oreh, smreka, brest). S pomoÄjo termoÄlena smo raziskali spreminjanje temperature v lepilnem spoju med vroÄim lepljenjem. Ugotovili smo, da je imela temperatura stiskanja bistveni vpliv na hitrost utrjevanja lepila in da hlajenje preizkuĆĄanca po stiskanju ni vplivalo na trdnost spoja. Lepilo z viĆĄjim deleĆŸem formaldehida je utrjevalo hitreje.We studied the effect of pressing temperature on hardening of urea-formaldehyde (UF) adhesives. We used two types of UF adhesives pre-prepared by manufacturer Basf: Kaurit 345 with a lower content of free formaldehyde and Kaurit 350 with a higher content. For testing of kinetics, we used ABES (Automated Bonding Evaluation System) instrument. To preform standard tests, we used maple veneers, with thickness of 0,6 mm. All veneer was prepared and then left for two day at room conditions of 23 °C and 55 % air humidity, to acclimate. Every test has constant adhesive mixture, consisted of 100 weight units of aqueous adhesive solution and 1,5 % catalyst (ammonium sulphate) (according to dry quantity of glue). Bonding strength was investigated at different pressing temperatures: 80, 100, 120, 150 and 170 °C. Times of gluing were adjusted according to feedback of shear strength. If ABES measured sear strength higher than 0 N/mm2, we carried out at least two more tests of strength. pH value of wood was measured. With usage of different veneers, we tested its influence on bond development. For this test, we used six different species (maple, beech, oak, walnut, spruce, elm). With use of thermocouple we investigated temperature changes in bond during gluing process. We found out that the pressing temperature had a significant influence on hardening time. Cooling specimens after gluing process did not have big effect on joint strength. Adhesive with more formaldehyde was curing faster
The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae
<p>Abstract</p> <p>Background</p> <p>The <it>Xanthomonadaceae </it>family contains two xylem-limited plant pathogenic bacterial species, <it>Xanthomonas albilineans </it>and <it>Xylella fastidiosa</it>. <it>X. fastidiosa </it>was the first completely sequenced plant pathogen. It is insect-vectored, has a reduced genome and does not possess <it>hrp </it>genes which encode a Type III secretion system found in most plant pathogenic bacteria. <it>X. fastidiosa </it>was excluded from the <it>Xanthomonas </it>group based on phylogenetic analyses with rRNA sequences.</p> <p>Results</p> <p>The complete genome of <it>X. albilineans </it>was sequenced and annotated. <it>X. albilineans</it>, which is not known to be insect-vectored, also has a reduced genome and does not possess <it>hrp </it>genes. Phylogenetic analysis using <it>X. albilineans </it>genomic sequences showed that <it>X. fastidiosa </it>belongs to the <it>Xanthomonas </it>group. Order of divergence of the <it>Xanthomonadaceae </it>revealed that <it>X. albilineans </it>and <it>X. fastidiosa </it>experienced a convergent reductive genome evolution during their descent from the progenitor of the <it>Xanthomonas </it>genus. Reductive genome evolutions of the two xylem-limited <it>Xanthomonadaceae </it>were compared in light of their genome characteristics and those of obligate animal symbionts and pathogens.</p> <p>Conclusion</p> <p>The two xylem-limited <it>Xanthomonadaceae</it>, during their descent from a common ancestral parent, experienced a convergent reductive genome evolution. Adaptation to the nutrient-poor xylem elements and to the cloistered environmental niche of xylem vessels probably favoured this convergent evolution. However, genome characteristics of <it>X. albilineans </it>differ from those of <it>X. fastidiosa </it>and obligate animal symbionts and pathogens, indicating that a distinctive process was responsible for the reductive genome evolution in this pathogen. The possible role in genome reduction of the unique toxin albicidin, produced by <it>X. albilineans</it>, is discussed.</p
Update of the Scientific Opinion on the risks to plant health posed by Xylella fastidiosa in the EU territory
EFSA was asked to update the 2015 EFSA risk assessment on Xylella fastidiosa for the territory of the EU. In particular, EFSA was asked to focus on potential establishment, shortâ and longârange spread, the length of the asymptomatic period, the impact of X. fastidiosa and an update on risk reduction options. EFSA was asked to take into account the different subspecies and Sequence Types of X. fastidiosa. This was attempted throughout the scientific opinion but several issues with data availability meant that this could only be partially achieved. Models for risk of establishment showed most of the EU territory may be potentially suitable for X. fastidiosa although southern EU is most at risk. Differences in estimated areas of potential establishment were evident among X. fastidiosa subspecies, particularly X. fastidiosa subsp. multiplex which demonstrated areas of potential establishment further north in the EU. The model of establishment could be used to develop targeted surveys by Member States. The asymptomatic period of X. fastidiosa varied significantly for different host and pathogen subspecies combinations, for example from a median of approximately 1 month in ornamental plants and up to 10 months in olive, for pauca. This variable and long asymptomatic period is a considerable limitation to successful detection and control, particularly where surveillance is based on visual inspection. Modelling suggested that local eradication (e.g. within orchards) is possible, providing sampling intensity is sufficient for early detection and effective control measures are implemented swiftly (e.g. within 30 days). Modelling of longârange spread (e.g. regional scale) demonstrated the important role of longârange dispersal and the need to better understand this. Reducing buffer zone width in both containment and eradication scenarios increased the area infected. Intensive surveillance for early detection, and consequent plant removal, of new outbreaks is crucial for both successful eradication and containment at the regional scale, in addition to effective vector control. The assessment of impacts indicated that almond and Citrus spp. were at lower impact on yield compared to olive. Although the lowest impact was estimated for grapevine, and the highest for olive, this was based on several assumptions including that the assessment considered only Philaenus spumarius as a vector. If other xylemâfeeding insects act as vectors the impact could be different. Since the Scientific Opinion published in 2015, there are still no risk reduction options that can remove the bacterium from the plant in open field conditions. Shortâ and longârange spread modelling showed that an early detection and rapid application of phytosanitary measures, consisting among others of plant removal and vector control, are essential to prevent further spread of the pathogen to new areas. Further data collection will allow a reduction in uncertainty and facilitate more tailored and effective control given the intraspecific diversity of X. fastidiosa and wide host range.Additional co-authors: EFSA Panel on Plant Health (PLH), Wopke van der Werf, Antonio Vicent Civera, Jonathan Yuen, Lucia ZappalĂ , Donato Boscia, Gianni Gilioli, Rodrigo Krugner, Alexander Mastin, Anna Simonetto, Joao Roberto Spotti Lopes, Steven White, JosĂ© Cortinas Abrahantes, Alice Delbianco, Andrea Maiorano, Olaf MosbachâSchulz, Giuseppe Stancanelli, Michela Guzzo, Stephen Parnel
Pest categorisation of Conotrachelus nenuphar
The EFSA Panel on Plant Health performed a pest categorisation of Conotrachelus nenuphar (Herbst)
(Coleoptera: Curculionidae), for the EU. C. nenuphar is a well-defined species, recognised as a serious
pest of stone and pome fruit in the USA and Canada where it also feeds on a range of other hosts
including soft fruit (e.g. Ribes, Fragaria) and wild plants (e.g. Crataegus). Adults, which are not good
flyers, feed on tender twigs, flower buds and leaves. Females oviposit into host fruit; if oviposition
occurs in young fruit, the fruit usually falls prematurely reducing yield; oviposition in older fruit causes
surface blemishes and the fruit distorts as it develops reducing marketability. Larvae develop within
host fruit but exit to pupate in soil. Adults overwinter in leaf litter. C. nenuphar is not known to occur
in the EU and is listed in Annex IAI of Council Directive 2000/29/EC. Fruit infested shortly before
harvest and soil with leaf litter accompanying plants for planting could potentially provide a pathway
into the EU. Considering the climatic similarities between North America and Europe, and that hosts
occur widely within the EU, C. nenuphar has potential to establish within the EU. There could be one
or two generations per year, as in North America. Impacts could be expected, e.g. in Prunus spp. and
apples. Phytosanitary measures are available to reduce the likelihood of introduction of C. nenuphar.
All of the criteria assessed by EFSA for consideration as a potential Union quarantine pest are met.
C. nenuphar does not meet the criteria of occurring in the EU nor plants for planting being the
principal means of spread. Hence it does not satisfy all of the criteria that are within the remit of EFSA
to assess for it to be regarded as a Union regulated non-quarantine pest (RNQP)
Pest categorisation of Sternochetus mangiferae
The European Commission requested EFSA to conduct a pest categorisation of Sternochetus mangiferae
(Coleoptera: Curculionidae), a monophagous pest weevil whose larvae exclusively feed on mango seeds,
whereas adults feed on mango foliage. S. mangiferae is a species with reliable methods available for
identification. It is regulated in the EU by Council Directive 2000/29/EC where it is listed in Annex IIB as a
harmful organism whose introduction into EU Protected Zones (PZ) (Alentejo, Algarve and Madeira in
Portugal, and Granada and Malaga in Spain) is banned. S. mangiferae is native to South East Asia and
has spread to other mango-growing areas in Africa, South America and Oceania, causing significant
damage. Larvae of S. mangiferae have been detected several times in mango fruit imported into the EU.
In 2013, an outbreak was declared in one PZ in Spain. Official measures taken achieved eradication,
which was officially declared in January 2018. The EFSA Plant Health Panel concludes that S. mangiferae
could establish again and spread in the mango-growing areas of southern EU. Considering the criteria
within the remit of EFSA to assess the status as a potential Union quarantine pest (QP), as a potential
protected zone quarantine pest (PZQP) or as a potential regulated non-quarantine pest (RNQP),
S. mangiferae meets with no uncertainties the criteria for consideration as a potential Union QP, as it is
absent from the EU, potential pathways for entry exist, and its establishment would cause an economic
impact. The criterion of the pest being present in the EU, which is a prerequisite for RNQP and PZ QP, is
not met
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