Isaiah 5: A prophetic critique of economic proportion

The text of Isaiah 5 describes a rapidly changing society in 8th-century BCE Judah. This shift is driven by forces such as increased international trade, the clash of urbanisation and agrarianism, and the rise of the urban elites. These changes have direct correlation with the transforming economic environment of that period. Yet scholars have paid little attention to the economic realities reflected in the message of the prophet Isaiah. This article explores the context of Isaiah 5 through an engagement with economic theory. While recognising the dilemmas in the adoption of tools from contemporary economic theory to analyse an ancient society, this article utilises game theory to shed light on the historical and cultural background to the text. By applying the ‘prisoner’s dilemma’ it explores the ‘deal’ being offered by the prophet to the Judahite leaders in Isaiah 5: 8–24. In particular, the article looks at rational choice and the self-interested basis of decision-making to draw insights from the text by highlighting the conflicts of interest for the wealthy elite of Isaiah 5. Intradisciplinary and/or interdisciplinary implications: This research relates to the disciplines of Old Testament Studies and Economic Theory. The focus is applying economic theory to the biblical text of Isaiah 5

Two Prp19-Like U-Box Proteins in the MOS4-Associated Complex Play Redundant Roles in Plant Innate Immunity

Plant Resistance (R) proteins play an integral role in defense against pathogen infection. A unique gain-of-function mutation in the R gene SNC1, snc1, results in constitutive activation of plant immune pathways and enhanced resistance against pathogen infection. We previously found that mutations in MOS4 suppress the autoimmune phenotypes of snc1, and that MOS4 is part of a nuclear complex called the MOS4-Associated Complex (MAC) along with the transcription factor AtCDC5 and the WD-40 protein PRL1. Here we report the immuno-affinity purification of the MAC using HA-tagged MOS4 followed by protein sequence analysis by mass spectrometry. A total of 24 MAC proteins were identified, 19 of which have predicted roles in RNA processing based on their homology to proteins in the Prp19-Complex, an evolutionarily conserved spliceosome-associated complex containing homologs of MOS4, AtCDC5, and PRL1. Among these were two highly similar U-box proteins with homology to the yeast and human E3 ubiquitin ligase Prp19, which we named MAC3A and MAC3B. MAC3B was recently shown to exhibit E3 ligase activity in vitro. Through reverse genetics analysis we show that MAC3A and MAC3B are functionally redundant and are required for basal and R protein–mediated resistance in Arabidopsis. Like mos4-1 and Atcdc5-1, mac3a mac3b suppresses snc1-mediated autoimmunity. MAC3 localizes to the nucleus and interacts with AtCDC5 in planta. Our results suggest that MAC3A and MAC3B are members of the MAC that function redundantly in the regulation of plant innate immunity

A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.

The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time

Isaiah’s Vision of Yahweh and Ethical Replication

This article explores Isaiah’s vision of the thrice-holy Yahweh and his message of ethical replication. According to Elaine Scarry, the presence of beauty prompts replication. This will be adapted to consider the role of holiness as a driving factor in Isaiah’s message of social justice. First, Isaiah is de-centred in his experience of Yahweh’s beauty and terror. Secondly, Isaiah volunteered to embody the message of Yahweh and to call his community to similarly mirror Yahweh in their religious and moral life. By focusing on the use of Isaiah’s key terminology of “the Holy One of Israel” in Isaiah 1-39, the expectation of ethical replication among the covenant community is examined. Thirdly, as this key term is also prevalent in chapters 40-66, arguably the expectation for ethical replication was continued by the disciples of Isaiah. This study reinforces holiness as having ethical as well as religious importance in Isaiah’s message

Global dataset of soil organic carbon in tidal marshes

Funding: W.E.N.A. and C.S. would like to acknowledge funding support from the Scottish Government and UK Natural Environment Research Council C-SIDE project (grant NE/R010846/1).Tidal marshes store large amounts of organic carbon in their soils. Field data quantifying soil organic carbon (SOC) stocks provide an important resource for researchers, natural resource managers, and policy-makers working towards the protection, restoration, and valuation of these ecosystems. We collated a global dataset of tidal marsh soil organic carbon (MarSOC) from 99 studies that includes location, soil depth, site name, dry bulk density, SOC, and/or soil organic matter (SOM). The MarSOC dataset includes 17,454 data points from 2,329 unique locations, and 29 countries. We generated a general transfer function for the conversion of SOM to SOC. Using this data we estimated a median (± median absolute deviation) value of 79.2±38.1 Mg SOC ha−1 in the top 30cm and 231±134 Mg SOC ha−1 in the top 1m of tidal marsh soils globally. This data can serve as a basis for future work, and may contribute to incorporation of tidal marsh ecosystems into climate change mitigation and adaptation strategies and policies.Publisher PDFPeer reviewe

Global dataset of soil organic carbon in tidal marshes.

Funder: The Nature Conservancy through the Bezos Earth Fund and other donor supportFunder: Nelson Mandela UniversityFunder: State Research Agency of Spain (AEI; CGL2007-64915), the Mancomunidad de los Canales del Taibilla (MCT), and the Science and Technology Agency of the Murcia Region (Seneca Foundation; 00593/PI/04 & 08739/PI/08).Funder: Scottish Government and UK Natural Environment Research Council C-SIDE project (grant NE/R010846/1)Funder: COOLSTYLE/CARBOSTORE projectFunder: New Zealand Ministry for Business, Innovation and Employment Contract #C01X2109Funder: Portuguese national funds from FCT - Foundation for Science and Technology through projects UIDB/04326/2020, UIDP/04326/2020, LA/P/0101/2020, and 2020.03825.CEECINDFunder: German Research Foundation (DFG project number: GI 171/25-1)Funder: State Research Agency of Spain (AEI; CGL2007-64915), the Mancomunidad de los Canales del Taibilla (MCT), the Science and Technology Agency of the Murcia Region (Seneca Foundation; 00593/PI/04 & 08739/PI/08), and a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation (RYC2020-029322-I)Funder: Velux foundation (#28421, Blå Skove – Havets Skove som kulstofdræn)Funder: LIFE ADAPTA BLUES project Ref. LIFE18 CCA/ES/001160Funder: LIFE ADAPTA BLUES project Ref. LIFE18 CCA/ES/001160, support of national funds through Fundação para a Ciência e Tecnologia, I.P. (FCT), under the projects UIDB/04292/2020, UIDP/04292/2020, granted to MARE, and LA/P/0069/2020, granted to the Associate Laboratory ARNETFunder: Financial support provided by the Welsh Government and Higher Education Funding Council for Wales through the Sêr Cymru National Research Network for Low Carbon, Energy and Environment; as well as the Spanish Ministry of Science and Innovation (project PID2020-113745RB-I00) and FEDERFunder: South African Department of Science and Innovation (DSI)—National Research Foundation (NRF) Research Chair in Shallow Water Ecosystems (UID: 84375), and the Nelson Mandela UniversityFunder: I+D+i projects RYC2019-027073-I and PIE HOLOCENO 20213AT014 funded by MCIN/AEI/10.13039/501100011033 and FEDERFunder: Funding support from the Scottish Government and UK Natural Environment Research Council C-SIDE project (grant NE/R010846/1)Funder: Xunta de Galicia (GRC project IN607A 2021-06)Funder: U.S. Army Engineering, Research and Development Center (ACTIONS project, W912HZ2020070)Tidal marshes store large amounts of organic carbon in their soils. Field data quantifying soil organic carbon (SOC) stocks provide an important resource for researchers, natural resource managers, and policy-makers working towards the protection, restoration, and valuation of these ecosystems. We collated a global dataset of tidal marsh soil organic carbon (MarSOC) from 99 studies that includes location, soil depth, site name, dry bulk density, SOC, and/or soil organic matter (SOM). The MarSOC dataset includes 17,454 data points from 2,329 unique locations, and 29 countries. We generated a general transfer function for the conversion of SOM to SOC. Using this data we estimated a median (± median absolute deviation) value of 79.2 ± 38.1 Mg SOC ha-1 in the top 30 cm and 231 ± 134 Mg SOC ha-1 in the top 1 m of tidal marsh soils globally. This data can serve as a basis for future work, and may contribute to incorporation of tidal marsh ecosystems into climate change mitigation and adaptation strategies and policies

Prospective observational cohort study on grading the severity of postoperative complications in global surgery research

Background The Clavien–Dindo classification is perhaps the most widely used approach for reporting postoperative complications in clinical trials. This system classifies complication severity by the treatment provided. However, it is unclear whether the Clavien–Dindo system can be used internationally in studies across differing healthcare systems in high- (HICs) and low- and middle-income countries (LMICs). Methods This was a secondary analysis of the International Surgical Outcomes Study (ISOS), a prospective observational cohort study of elective surgery in adults. Data collection occurred over a 7-day period. Severity of complications was graded using Clavien–Dindo and the simpler ISOS grading (mild, moderate or severe, based on guided investigator judgement). Severity grading was compared using the intraclass correlation coefficient (ICC). Data are presented as frequencies and ICC values (with 95 per cent c.i.). The analysis was stratified by income status of the country, comparing HICs with LMICs. Results A total of 44 814 patients were recruited from 474 hospitals in 27 countries (19 HICs and 8 LMICs). Some 7508 patients (16·8 per cent) experienced at least one postoperative complication, equivalent to 11 664 complications in total. Using the ISOS classification, 5504 of 11 664 complications (47·2 per cent) were graded as mild, 4244 (36·4 per cent) as moderate and 1916 (16·4 per cent) as severe. Using Clavien–Dindo, 6781 of 11 664 complications (58·1 per cent) were graded as I or II, 1740 (14·9 per cent) as III, 2408 (20·6 per cent) as IV and 735 (6·3 per cent) as V. Agreement between classification systems was poor overall (ICC 0·41, 95 per cent c.i. 0·20 to 0·55), and in LMICs (ICC 0·23, 0·05 to 0·38) and HICs (ICC 0·46, 0·25 to 0·59). Conclusion Caution is recommended when using a treatment approach to grade complications in global surgery studies, as this may introduce bias unintentionally

The surgical safety checklist and patient outcomes after surgery: a prospective observational cohort study, systematic review and meta-analysis

© 2017 British Journal of Anaesthesia Background: The surgical safety checklist is widely used to improve the quality of perioperative care. However, clinicians continue to debate the clinical effectiveness of this tool. Methods: Prospective analysis of data from the International Surgical Outcomes Study (ISOS), an international observational study of elective in-patient surgery, accompanied by a systematic review and meta-analysis of published literature. The exposure was surgical safety checklist use. The primary outcome was in-hospital mortality and the secondary outcome was postoperative complications. In the ISOS cohort, a multivariable multi-level generalized linear model was used to test associations. To further contextualise these findings, we included the results from the ISOS cohort in a meta-analysis. Results are reported as odds ratios (OR) with 95% confidence intervals. Results: We included 44 814 patients from 497 hospitals in 27 countries in the ISOS analysis. There were 40 245 (89.8%) patients exposed to the checklist, whilst 7508 (16.8%) sustained ≥1 postoperative complications and 207 (0.5%) died before hospital discharge. Checklist exposure was associated with reduced mortality [odds ratio (OR) 0.49 (0.32–0.77); P\u3c0.01], but no difference in complication rates [OR 1.02 (0.88–1.19); P=0.75]. In a systematic review, we screened 3732 records and identified 11 eligible studies of 453 292 patients including the ISOS cohort. Checklist exposure was associated with both reduced postoperative mortality [OR 0.75 (0.62–0.92); P\u3c0.01; I2=87%] and reduced complication rates [OR 0.73 (0.61–0.88); P\u3c0.01; I2=89%). Conclusions: Patients exposed to a surgical safety checklist experience better postoperative outcomes, but this could simply reflect wider quality of care in hospitals where checklist use is routine