Beplanningsevaluering van openbare diensaanbod: Onderwysgeriewe in die Kaapprovinsie

The effective and equal provision of quality public services to the total population requires continual evalua­tion and monitoring of distribution practises and patterns to eliminate discriminatory resource allocation through planning action. With the Coloured education system in the Cape Province as example, a proce­dure for the evaluation of spatial resource distribution at the district level is demonstrated. Initially the selected range of variables and their significance as system indicators is discussed. These variables are then factor analysed to isolate the main underlying dimensions which summar­ize spatial variations in facility alloca­tion in the province. Representative variables are identified for less cumber­some future evaluation and monitor­ing of the system. Districts are classi­fied through cluster analysis to identify district groups/ regions experiencing similar characteristics/ problems in need of specific planning action. The implementation of an integrated spa­tial data base (a geographical informa­tion system) is recommended as a tool to facilitate the planning of public service delivery. This would enable the procedure demonstrated to be applied at the micro scale for the evaluation of service allocation within local author­iiy areas as well. *This article is written in Afrikaans

The hydrodynamics of the Bot River Estuary revisited

For the past 20 years management of the Bot/Kleinmond estuarine system in the south-western Cape has been based on the premise that, barring intervention, the estuary was naturally evolving into a freshwater coastal lake. This paper presents evidence, based on a 20-year series of water-level data, updated runoff estimates from the catchment and dimensional data, that, in the absence of anthropogenic influences, the system is not progressing naturally, but artificially, towards becoming a freshwater system. It is concluded that the increasingly closed state of the Bot Estuary in recent years is most likely due to reduction in runoff from its tributaries and premature artificial breaching of the Kleinmond arm of the system. These findings, coupled with the high conservation importance of the Bot River Estuary, suggest that the current management plan needs urgent revaluation and that the two estuaries cannot be managed separately. Key words: Bot River Estuary, estuary management, estuary breaching policy, closed estuary, estuarine dimensions, estuarine water levels, estuarine habitat Water SA Vol.31(1) 2005: 73-8

STIGMATISATION OF HIV-POSITIVE MEMBERS OF THE WORKFORCE

The rate at which the Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) is spreading throughout the world, especially Africa, is alarming. Although Africa contains only about 12% of the world’s population, it contains over 70% of HIV-infected individuals. It is estimated that over 10% of the South African population is HIV positive (Addison, 2001). This pandemic is a threat to the economy, individual workers and society in general (House, Eicken & Gray, 1995). Social implications of HIV/AIDS include fear, prejudice, discrimination, hysteria, stigmatisation and other irrational responses from members of society (Herek & Glunt, 1988; House et al., 1995). For the purpose of this study, "stigmatisation" refers to the irrational responses (including fear, attitudes and prejudicial behaviour) by co-workers and/or superiors within the work environment towards HIV positive individuals

Gastric cancers of Western European and African patients show different patterns of genomic instability

<p>Abstract</p> <p>Background</p> <p>Infection with <it>H. pylori </it>is important in the etiology of gastric cancer. Gastric cancer is infrequent in Africa, despite high frequencies of <it>H. pylori </it>infection, referred to as the African enigma. Variation in environmental and host factors influencing gastric cancer risk between different populations have been reported but little is known about the biological differences between gastric cancers from different geographic locations. We aim to study genomic instability patterns of gastric cancers obtained from patients from United Kingdom (UK) and South Africa (SA), in an attempt to support the African enigma hypothesis at the biological level.</p> <p>Methods</p> <p>DNA was isolated from 67 gastric adenocarcinomas, 33 UK patients, 9 Caucasian SA patients and 25 native SA patients. Microsatellite instability and chromosomal instability were analyzed by PCR and microarray comparative genomic hybridization, respectively. Data was analyzed by supervised univariate and multivariate analyses as well as unsupervised hierarchical cluster analysis.</p> <p>Results</p> <p>Tumors from Caucasian and native SA patients showed significantly more microsatellite instable tumors (p < 0.05). For the microsatellite stable tumors, geographical origin of the patients correlated with cluster membership, derived from unsupervised hierarchical cluster analysis (p = 0.001). Several chromosomal alterations showed significantly different frequencies in tumors from UK patients and native SA patients, but not between UK and Caucasian SA patients and between native and Caucasian SA patients.</p> <p>Conclusions</p> <p>Gastric cancers from SA and UK patients show differences in genetic instability patterns, indicating possible different biological mechanisms in patients from different geographical origin. This is of future clinical relevance for stratification of gastric cancer therapy.</p

Variation in the Analysis of Positively Selected Sites Using Nonsynonymous/Synonymous Rate Ratios: An Example Using Influenza Virus

Sites in a gene showing the nonsynonymous/synonymous rate ratio (ω) >1 have been frequently identified to be under positive selection. To examine the performance of such analysis, sites of the ω ratio >1 in the HA1 gene of H3N2 subtype human influenza viruses were identified from seven overlapping sequence data sets in this study. Our results showed that the sites of the ω ratio >1 were of significant variation among the data sets even though they targeted similar clusters, indicating that the analysis is likely to be either of low sensitivity or of low specificity in identifying sites under positive selection. Most (43/45) of the sites showing ω >1 calculated from at least one data set are involved in B-cell epitopes which cover less than a half sites in the protein, suggesting that the analysis is likely to be of low sensitivity rather than of low specificity. It was further found that the analysis sensitivity could not be enhanced by including more sequences or covering longer time intervals. Previously some reports also likely identified only a portion of the sites under positive selection in the viral gene using the ω ratio. Low sensitivity of the analysis may result from that some sites under positive selection in the gene are also under negative (purifying) selection simultaneously for functional constrains, and so their ω ratios could be <1. Theoretically, the sites under the two opposite selection forces at the same time favor only certain nonsynonymous changes, e.g. those changing the antigenicity of the gene and maintaining the gene function. This study also suggested that sometimes we can identify more sites under positive selection using the ω ratio by integrating the positively selected sites estimated from multiple data sets

Initiation of T cell signaling by CD45 segregation at 'close contacts'.

It has been proposed that the local segregation of kinases and the tyrosine phosphatase CD45 underpins T cell antigen receptor (TCR) triggering, but how such segregation occurs and whether it can initiate signaling is unclear. Using structural and biophysical analysis, we show that the extracellular region of CD45 is rigid and extends beyond the distance spanned by TCR-ligand complexes, implying that sites of TCR-ligand engagement would sterically exclude CD45. We also show that the formation of 'close contacts', new structures characterized by spontaneous CD45 and kinase segregation at the submicron-scale, initiates signaling even when TCR ligands are absent. Our work reveals the structural basis for, and the potent signaling effects of, local CD45 and kinase segregation. TCR ligands have the potential to heighten signaling simply by holding receptors in close contacts.The authors thank R.A. Cornall, M.L. Dustin and P.A. van der Merwe for comments on the manuscript and S. Ikemizu for useful discussions about the structure. We also thank W. Lu and T. Walter for technical support with protein expression and crystallization, the staff at Diamond Light Source beamlines I02, I03 and I04-1 (proposal mx10627) and European Synchrotron Radiation Facility beamlines ID23EH1 and ID23EH2 for assistance at the synchrotrons, G. Sutton for assistance with MALS experiments, and M. Fritzsche for advice on the calcium analysis. This work was funded by the Wellcome Trust (098274/Z/12/Z to S.J.D.; 090532/Z/09/Z to R.J.C.G.; 090708/Z/09/Z to D.K.), the UK Medical Research Council (G0700232 to A.R.A.), the Royal Society (UF120277 to S.F.L.) and Cancer Research UK (C20724/A14414 to C.S.; C375/A10976 to E.Y.J.). The Oxford Division of Structural Biology is part of the Wellcome Trust Centre for Human Genetics, Wellcome Trust Core Award Grant Number 090532/Z/09/Z. We acknowledge financial support from Instruct, an ESFRI Landmark Project. The OPIC electron microscopy facility was funded by a Wellcome Trust JIF award (060208/Z/00/Z).This is the author accepted manuscript. The final version is available from Nature Publishing Group via https://doi.org/10.1038/ni.339

A kinetic and thermodynamic investigation into the removal of methyl orange from wastewater utilizing fly ash in different process configurations

The removal of methyl orange using coal fly ash, which is a widely available low-cost adsorbent, has been investigated. Adsorption studies for dye removal were conducted using various configurations such as batch, column and heap adsorption at various temperatures and adsorbent dosages at neutral pH. The Langmuir, Freundlich and Tempkin isotherm models were used to describe the process. The Freundlich model best represented the adsorption. Kinetic studies show the adsorption followed pseudo-second-order kinetics. Thermodynamic studies show that the process is spontaneous, endothermic and random. Column configuration was found to be the most efficient with a dye removal percentage of 99.95%, followed by heap adsorption at 99.25% removal and lastly batch configuration with 96.68% removal. Economic analysis shows that column operation would be the most effective for practical implementation

The gist of instructional leadership practised in Swaziland primary schools

A growing body of scholarship links instructional leadership to effective teaching and learning. This article looks at the ‘what’ of instructional leadership as practised in Swaziland primary schools. A qualitative investigation was undertaken based on individual and focus group interviews conducted at eight primary schools in the Hhohho region of Swaziland. The findings show that demonstrative leadership accompanied by collaborative support and recognition for achievement are important features of an effective instructional leadership programme. The main limitations to optimal learning are the collection of school fees during school hours and balancing English as the language of instruction with preserving the indigenous language. The findings emphasize the importance of mutual effort as the main component of effective teaching and learning.IS

Downregulation of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose-phosphate levels

Analyses of transgenic sugarcane clones with 45–95% reduced cytosolic pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) activity displayed no visual phenotypical change, but significant changes were evident in in vivo metabolite levels and fluxes during internode development. In three independent transgenic lines, sucrose concentrations increased between three- and sixfold in immature internodes, compared to the levels in the wildtype control. There was an eightfold increase in the hexose-phosphate:triose-phosphate ratio in immature internodes, a significant restriction in the triose phosphate to hexose phosphate cycle and significant increase in sucrose cycling as monitored by 13C nuclear magnetic resonance. This suggests that an increase in the hexose-phosphate concentrations resulting from a restriction in the conversion of hexose phosphates to triose phosphates drive sucrose synthesis in the young internodes. These effects became less pronounced as the tissue matured. Decreased expression of PFP also resulted in an increase of the ATP/ADP and UTP/UDP ratios, and an increase of the total uridine nucleotide and, at a later stage, the total adenine nucleotide pool, revealing strong interactions between PPi metabolism and general energy metabolism. Finally, decreased PFP leads to a reduction of PPi levels in older internodes indicating that in these developmental stages PFP acts in the gluconeogenic direction. The lowered PPi levels might also contribute to the absence of increases in sucrose contents in the more mature tissues of transgenic sugarcane with reduced PFP activity