RSPO3 impacts body fat distribution and regulates adipose cell biology in vitro

Fat distribution is an independent cardiometabolic risk factor. However, its molecular and cellular underpinnings remain obscure. Here we demonstrate that two independent GWAS signals at RSPO3, which are associated with increased body mass index-adjusted waist-to-hip ratio, act to specifically increase RSPO3 expression in subcutaneous adipocytes. These variants are also associated with reduced lower-body fat, enlarged gluteal adipocytes and insulin resistance. Based on human cellular studies RSPO3 may limit gluteofemoral adipose tissue (AT) expansion by suppressing adipogenesis and increasing gluteal adipocyte susceptibility to apoptosis. RSPO3 may also promote upper-body fat distribution by stimulating abdominal adipose progenitor (AP) proliferation. The distinct biological responses elicited by RSPO3 in abdominal versus gluteal APs in vitro are associated with differential changes in WNT signalling. Zebrafish carrying a nonsense rspo3 mutation display altered fat distribution. Our study identifies RSPO3 as an important determinant of peripheral AT storage capacity

The polarization of mm methanol masers

We present a survey of the polarization properties of mm-wavelength methanol masers, comprising both classes, and transitions from 84.5 to 157.0 GHz. Linear polarization is found in more than half of the observed objects, and circular polarization is tentatively detected in two sources. Class I and Class II CH_3OH masers show similar polarization properties.The largest linear polarization is found in the 133 GHz Class I maser towards L379 (39.5 %), and in the 157 GHz Class II maser towards G9.62+0.19 (36.7 %). The spectral profiles of the polarization angle of Class I masers are mostly flat, except for two sources showing a linear slope. Since the mm-line methanol masers are expected to be weakly (or not) saturated, we suggest that the stronger fractional polarizations found by us are enhanced by anisotropic pumping and radiative losses. In NGC 7538, we find, for both maser classes, a good agreement between our polarization angles, and those measured for the submillimeter dust continuum. This can be taken as evidence for magnetic alignment of dust grains. It is also possible that an unsaturated maser with equally populated magnetic substates simply amplifies polarized continuum seed radiation. For Class II masers, the polarization properties of the various velocity components towards a given source with detectable polarization are quite homogeneous. A possible explanation is discussed. Since methanol is non-paramagnetic, the circular polarization of the unsaturated maser emission can only be due to variations of the angle between the magnetic field and the line of sight along the maser propagation path.Comment: Astronomy & Astrophysics, in press, received 27 February 2004, accepted 27 July 200

Unusual development of light-reflecting pigment cells in intact and regenerating tail in the periodic albino mutant of Xenopus laevis

Unusual light-reflecting pigment cells, “white pigment cells”, specifically appear in the periodic albino mutant (ap/ap) of Xenopus laevis and localize in the same place where melanophores normally differentiate in the wild-type. The mechanism responsible for the development of unusual pigment cells is unclear. In this study, white pigment cells in the periodic albino were compared with melanophores in the wild-type, using a cell culture system and a tail-regenerating system. Observations of both intact and cultured cells demonstrate that white pigment cells are unique in (1) showing characteristics of melanophore precursors at various stages of development, (2) accumulating reflecting platelets characteristic of iridophores, and (3) exhibiting pigment dispersion in response to α-melanocyte stimulating hormone (α-MSH) in the same way that melanophores do. When a tadpole tail is amputated, a functionally competent new tail is regenerated. White pigment cells appear in the mutant regenerating tail, whereas melanophores differentiate in the wild-type regenerating tail. White pigment cells in the mutant regenerating tail are essentially similar to melanophores in the wild-type regenerating tail with respect to their localization, number, and response to α-MSH. In addition to white pigment cells, iridophores which are never present in the intact tadpole tail appear specifically in the somites near the amputation level in the mutant regenerating tail. Iridophores are distinct from white pigment cells in size, shape, blue light-induced fluorescence, and response to α-MSH. These findings strongly suggest that white pigment cells in the mutant arise from melanophore precursors and accumulate reflecting platelets characteristic of iridophores