The Cerevoice Blizzard Entry 2007: Are Small Database Errors Worse than Compression Artifacts?

In commercial systems the memory footprint of unit selection systems is often a key issue. This is especially true for PDAs and other embedded devices. In this years Blizzard entry CereProc R○gave itself the criteria that the full database system entered would have a smaller memory footprint than either of the two smaller database entries. This was accomplished by applying speex speech compression to the full database entry. In turn a set of small database techniques used to improve the quality of small database systems in last years entry were extended. Finally, for all systems, two quality control methods were applied to the underlying database to improve the lexicon and transcription match to the underlying data. Results suggest that mild audio quality artifacts introduced by lossy compression have almost as much impact on MOS perceived quality as concatenation errors introduced by sparse data in the smaller systems with bulked diphones. Index Terms: speech synthesis, unit selection. 1

The CSTR/Cereproc Blizzard Entry 2008: The Inconvenient Data

In a commercial system data used for unit selection systems is collected with a heavy emphasis on homogeneous neutral data that has sufficient coverage for the units that will be used in the system. In this years Blizzard entry CSTR and CereProc present a joint entry where the emphasis has been to explore techniques to deal with data which is not homogeneous (the English entry) and did not have appropriate coverage for a diphone based system (the Mandarin entry where tone/phone combinations were treated as distinct phone categories). In addition, two further problems were addressed, 1) Making use of non-homogeneous data for creating a voice that can realise both expressive and neutral speaking styles (the English entry) 2) Building a unit selection system with no native understanding of the language but depending instead on external native evaluation (the Mandarin Entry)

Glottal Source and Prosodic Prominence Modelling in HMM-based Speech Synthesis for the Blizzard Challenge 2009

This paper describes the CSTR entry for the Blizzard Challenge 2009. The work focused on modifying two parts of the Nitech 2005 HTS speech synthesis system to improve naturalness and contextual appropriateness. The first part incorporated an implementation of the Linjencrants-Fant (LF) glottal source model. The second part focused on improving synthesis of prosodic prominence including emphasis through context dependent phonemes. Emphasis was assigned to the synthesised test sentences based on a handful of theory based rules. The two parts (LF-model and prosodic prominence) were not combined and hence evaluated separately. The results on naturalness for the LF-model showed that it is not yet perceived as natural as the Benchmark HTS system for neutral speech. The results for the prosodic prominence modelling showed that it was perceived as contextually appropriate as the Benchmark HTS system, despite a low naturalness score. The Blizzard challenge evaluation has provided valuable information on the status of our work and continued work will begin with analysing why our modifications resulted in reduced naturalness compared to the Benchmark HTS system

Prediction and Realisation of Conversational Characteristics by Utilising Spontaneous Speech for Unit Selection

Unit selection speech synthesis has reached high levels of naturalness and intelligibility for neutral read aloud speech. However, synthetic speech generated using neutral read aloud data lacks all the attitude, intention and spontaneity associated with everyday conversations. Unit selection is heavily data dependent and thus in order to simulate human conversational speech, or create synthetic voices for believable virtual characters, we need to utilise speech data with examples of how people talk rather than how people read. In this paper we included carefully selected utterances from spontaneous conversational speech in a unit selection voice. Using this voice and by automatically predicting type and placement of lexical fillers and filled pauses we can synthesise utterances with conversational characteristics. A perceptual listening test showed that it is possible to make synthetic speech sound more conversational without degrading naturalness

Assessing the Ability of Spectroscopic Methods to Determine the Difference in the Folding Propensities of Highly Similar beta-Hairpins

We have evaluated the ability of nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopies to describe the difference in the folding propensities of two structurally highly similar cyclic β-hairpins, comparing the outcome to that of molecular dynamics simulations. NAMFIS-type NMR ensemble analysis and CD spectroscopy were observed to accurately describe the consequence of altering a single interaction site, whereas a single-site ¹³C NMR chemical shift melting curve-based technique was not

Many Body Theory of Charge Transfer in Hyperthermal Atomic Scattering

We use the Newns-Anderson Hamiltonian to describe many-body electronic processes that occur when hyperthermal alkali atoms scatter off metallic surfaces. Following Brako and Newns, we expand the electronic many-body wavefunction in the number of particle-hole pairs (we keep terms up to and including a single particle-hole pair). We extend their earlier work by including level crossings, excited neutrals and negative ions. The full set of equations of motion are integrated numerically, without further approximations, to obtain the many-body amplitudes as a function of time. The velocity and work-function dependence of final state quantities such as the distribution of ion charges and excited atomic occupancies are compared with experiment. In particular, experiments that scatter alkali ions off clean Cu(001) surfaces in the energy range 5 to 1600 eV constrain the theory quantitatively. The neutralization probability of Na$^+$ ions shows a minimum at intermediate velocity in agreement with the theory. This behavior contrasts with that of K$^+$, which shows ... (7 figures, not included. Figure requests: [email protected])Comment: 43 pages, plain TeX, BUP-JBM-

Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells.

The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.Wellcome Trust Royal Societ

Evolution and Global Transmission of a Multidrug-Resistant, Community-Associated Methicillin-Resistant Staphylococcus aureus Lineage from the Indian Subcontinent.

The evolution and global transmission of antimicrobial resistance have been well documented for Gram-negative bacteria and health care-associated epidemic pathogens, often emerging from regions with heavy antimicrobial use. However, the degree to which similar processes occur with Gram-positive bacteria in the community setting is less well understood. In this study, we traced the recent origins and global spread of a multidrug-resistant, community-associated Staphylococcus aureus lineage from the Indian subcontinent, the Bengal Bay clone (ST772). We generated whole-genome sequence data of 340 isolates from 14 countries, including the first isolates from Bangladesh and India, to reconstruct the evolutionary history and genomic epidemiology of the lineage. Our data show that the clone emerged on the Indian subcontinent in the early 1960s and disseminated rapidly in the 1990s. Short-term outbreaks in community and health care settings occurred following intercontinental transmission, typically associated with travel and family contacts on the subcontinent, but ongoing endemic transmission was uncommon. Acquisition of a multidrug resistance integrated plasmid was instrumental in the emergence of a single dominant and globally disseminated clade in the early 1990s. Phenotypic data on biofilm, growth, and toxicity point to antimicrobial resistance as the driving force in the evolution of ST772. The Bengal Bay clone therefore combines the multidrug resistance of traditional health care-associated clones with the epidemiological transmission of community-associated methicillin-resistant S. aureus (MRSA). Our study demonstrates the importance of whole-genome sequencing for tracking the evolution of emerging and resistant pathogens. It provides a critical framework for ongoing surveillance of the clone on the Indian subcontinent and elsewhere.IMPORTANCE The Bengal Bay clone (ST772) is a community-associated and multidrug-resistant Staphylococcus aureus lineage first isolated from Bangladesh and India in 2004. In this study, we showed that the Bengal Bay clone emerged from a virulent progenitor circulating on the Indian subcontinent. Its subsequent global transmission was associated with travel or family contact in the region. ST772 progressively acquired specific resistance elements at limited cost to its fitness and continues to be exported globally, resulting in small-scale community and health care outbreaks. The Bengal Bay clone therefore combines the virulence potential and epidemiology of community-associated clones with the multidrug resistance of health care-associated S. aureus lineages. This study demonstrates the importance of whole-genome sequencing for the surveillance of highly antibiotic-resistant pathogens, which may emerge in the community setting of regions with poor antibiotic stewardship and rapidly spread into hospitals and communities across the world