ICEF2004-960 MICROPHONES AND KNOCK SENSORS FOR FEEDBACK CONTROL OF HCCI ENGINES

ABSTRACT Homogeneous charge compression ignition (HCCI) engines lack direct in-cylinder CA50 engine crank position in CAD at 50% heat release CAD crank angle degrees HCCI homogeneous charge compression ignition µ sample mean ∇ differencing operator, ∇Y t = Y t −Y t−1 P t predicted (at engine position t) value of a series φ fuel-air equivalence ratio PID proportional-integral-derivative control law RPM revolutions per minute SI spark-ignited TDC top-dead-center of the compression stroke V voltage Y t time t values of data series WN(µ,σ 2 ) normally-distributed white noise process with mean µ and variance σ

Nonthermal Atmospheric Plasma Reactors for Hydrogen Production from Low-Density Polyethylene

Hydrogen is largely produced via natural gas reforming or electrochemical water-splitting, leaving organic solid feedstocks under-utilized. Plasma technology powered by renewable electricity can lead to the sustainable upcycling of plastic waste and production of green hydrogen. In this work, low-temperature atmospheric pressure plasma reactors based on transferred arc (transarc) and gliding arc (glidarc) discharges are designed, built, and characterized to produce hydrogen from low-density polyethylene (LDPE) as a model plastic waste. Experimental results show that hydrogen production rate and efficiency increase monotonically with increasing voltage level in both reactors, with the maximum hydrogen production of 0.33 and 0.42 mmol/g LDPE for transarc and glidarc reactors, respectively. For the transarc reactor, smaller electrode-feedstock spacing favors greater hydrogen production, whereas, for the glidarc reactor, greater hydrogen production is obtained at intermediate flow rates. The hydrogen production from LDPE is comparable despite the markedly different modes of operation between the two reactors

Hydrogen from Cellulose and Low-density Polyethylene via Atmospheric Pressure Nonthermal Plasma

The valorization of waste, by creating economic value while limiting environmental impact, can have an essential role in sustainable development. Particularly, polymeric waste such as biomass and plastics can be used for the production of green hydrogen as a carbon-free energy carrier through the use of nonthermal plasma powered by renewable, potentially surplus, electricity. In this study, a Streamer Dielectric-Barrier Discharge (SDBD) reactor is designed and built to extract hydrogen and carbon co-products from cellulose and low-density polyethylene (LDPE) as model feedstocks of biomass and plastic waste, respectively. Spectroscopic and electrical diagnostics, together with modeling, are used to estimate representative plasma properties, namely electron and excitation temperatures, number density, and power consumption. Cellulose and LDPE are plasma-treated for different treatment times to characterize the evolution of the hydrogen production process. Gas products are analyzed using gas chromatography to determine the mean hydrogen production rate, production efficiency, hydrogen yield, selectivity, and energy cost. The results show that the maximum hydrogen production efficiency for cellulose is 0.8 mol/kWh, which is approximately double that for LDPE. Furthermore, the energy cost of hydrogen production from cellulose is 600 kWh/kg of H2, half that of LDPE. Solid products are examined via scanning electron microscopy, revealing the distinct morphological structure of the two feedstocks treated, as well as by elemental composition analysis. The results demonstrate that SDBD plasma is effective at producing hydrogen from cellulose and LDPE at near atmospheric pressure and relatively low-temperature conditions in rapid-response and compact processes

Consumers across five European countries prioritise animal welfare above environmental sustainability when buying meat and dairy products

Food production systems, especially meat and dairy supply chains, contribute to greenhouse gas emissions. An important question emerges as to whether consumers care about environmental sustainability when buying food products, as this can determine their consumption practices. Further, if sustainability labels are available, identifying information that is relevant to consumers is important. This research therefore aimed to identify the attributes that are most important for consumers when buying meat or dairy products and the perceived helpfulness of sustainability labels for meat and dairy products and important label properties. An online survey was conducted in five European countries (i.e. Czechia, Spain, Sweden, Switzerland, and the UK). Consumers valued similar attributes when buying meat and dairy products across all countries. Freshness, quality/taste and animal welfare emerged as the most important attributes, while environmental attributes such as food miles, carbon footprint, and organic production were the least important. Sustainability labels for meat and dairy products were perceived as helpful. Regression analysis identified similar patterns within all five countries regarding the predictors of the perceived helpfulness of sustainability labels. Attitudes towards sustainable food consumption, environmental attitudes, and food production and policies emerged as significant positive predictors in most models. Most importantly, information regarding animal welfare, food safety, and health and nutrition was perceived as being more important than environmental sustainability. This suggests that food choice decisions are unlikely to be made based on the environmental sustainability of a food product’s production alone

Microparticle-mediated transfer of the viral receptors CAR and CD46, and the CFTR channel in a CHO cell model confers new functions to target cells

Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells. The MP-mediated, cell-to-cell transfer of three human membrane glycoproteins of different degrees of complexity was investigated in the present study, using a CHO cell model system. We first tested the delivery of CAR and CD46, two monospanins which act as adenovirus receptors, to target CHO cells. CHO cells lack CAR and CD46, high affinity receptors for human adenovirus serotype 5 (HAdV5), and serotype 35 (HAdV35), respectively. We found that MPs derived from CHO cells (MP-donor cells) constitutively expressing CAR (MP-CAR) or CD46 (MP-CD46) were able to transfer CAR and CD46 to target CHO cells, and conferred selective permissiveness to HAdV5 and HAdV35. In addition, target CHO cells incubated with MP-CD46 acquired the CD46-associated function in complement regulation. We also explored the MP-mediated delivery of a dodecaspanin membrane glycoprotein, the CFTR to target CHO cells. CFTR functions as a chloride channel in human cells and is implicated in the genetic disease cystic fibrosis. Target CHO cells incubated with MPs produced by CHO cells constitutively expressing GFP-tagged CFTR (MP-GFP-CFTR) were found to gain a new cellular function, the chloride channel activity associated to CFTR. Time-course analysis of the appearance of GFP-CFTR in target cells suggested that MPs could achieve the delivery of CFTR to target cells via two mechanisms: the transfer of mature, membrane-inserted CFTR glycoprotein, and the transfer of CFTR-encoding mRNA. These results confirmed that cell-derived MPs represent a new class of promising therapeutic vehicles for the delivery of bioactive macromolecules, proteins or mRNAs, the latter exerting the desired therapeutic effect in target cells via de novo synthesis of their encoded proteins

Tipping points and farmer decision-making in European permanent grassland (PG) agricultural systems

Permanent grasslands (PG) provide multifunctional ecosystem services (ES) in Europe and globally, which are threatened by both increased farming intensity and land use change in marginal areas. Farm management decisions can represent critical thresholds, or behavioural “tipping points”, in the agricultural system. Decisions are influenced by a combination of agronomic, policy and social factors. Transformation of PG systems can be facilitated through positive tipping points and relevant policy implementation to ensure sustainable PG systems. The aim of this research was to understand the drivers of decisions regarding land use changes and management towards critical positive and negative tipping points across five biogeographic zones in Europe. Interview methodology assessed farmers’ preferences and priorities regarding the adoption of sustainable PG systems. Participants were selected from five case study countries, each representing a different biogeographic zone in Europe (Continental/Pannonian: Czech Republic, Boreal: Sweden, Mediterranean: Spain, Alpine: Switzerland, and Atlantic: UK). The sample also covered three farming intensity types within these biogeographic zones: high input/intensive conventional farms (≥1.0 LU /ha); low input/extensive conventional farms (<1.0 LU/ha); and certified organic farms. In total, 373 farm interviews were obtained from the case study countries between October 2020 and October 2021. The analysis focuses on drivers of change and considers tipping points across these countries, considering case studies of land use changes (specifically land abandonment) and land management practices (specifically changes in stocking rates). The most common reasons for PG management changes towards either intensification or extensification were economic. Farmers require policy support to increase provision of non-market ES, while rebalancing subsidies can deliver environmental ES at scale through abandonment (e.g., through the creation of specific habitats that support some threatened species). Agri-environment schemes (AES)and subsidies could be more flexible to allow farmers to better adapt grassland management to local production conditions and unpredictable circumstances such as droughts, floods, or market shocks. To maintain PG that delivers more goods and services, financial compensation for ES delivery was perceived to be the most significant support mechanism needed, while easier access to ES provision expertise through extension or consultancy services is considered important factor

Transfer of MicroRNAs by Embryonic Stem Cell Microvesicles

Microvesicles are plasma membrane-derived vesicles released into the extracellular environment by a variety of cell types. Originally characterized from platelets, microvesicles are a normal constituent of human plasma, where they play an important role in maintaining hematostasis. Microvesicles have been shown to transfer proteins and RNA from cell to cell and they are also believed to play a role in intercellular communication. We characterized the RNA and protein content of embryonic stem cell microvesicles and show that they can be engineered to carry exogenously expressed mRNA and protein such as green fluorescent protein (GFP). We demonstrate that these engineered microvesicles dock and fuse with other embryonic stem cells, transferring their GFP. Additionally, we show that embryonic stem cells microvesicles contain abundant microRNA and that they can transfer a subset of microRNAs to mouse embryonic fibroblasts in vitro. Since microRNAs are short (21–24 nt), naturally occurring RNAs that regulate protein translation, our findings open up the intriguing possibility that stem cells can alter the expression of genes in neighboring cells by transferring microRNAs contained in microvesicles. Embryonic stem cell microvesicles may be useful therapeutic tools for transferring mRNA, microRNAs, protein, and siRNA to cells and may be important mediators of signaling within stem cell niches

Seed Dispersal Anachronisms: Rethinking the Fruits Extinct Megafauna Ate

Background: Some neotropical, fleshy-fruited plants have fruits structurally similar to paleotropical fruits dispersed by megafauna (mammals.10 3 kg), yet these dispersers were extinct in South America 10–15 Kyr BP. Anachronic dispersal systems are best explained by interactions with extinct animals and show impaired dispersal resulting in altered seed dispersal dynamics. Methodology/Principal Findings: We introduce an operational definition of megafaunal fruits and perform a comparative analysis of 103 Neotropical fruit species fitting this dispersal mode. We define two megafaunal fruit types based on previous analyses of elephant fruits: fruits 4–10 cm in diameter with up to five large seeds, and fruits.10 cm diameter with numerous small seeds. Megafaunal fruits are well represented in unrelated families such as Sapotaceae, Fabaceae, Solanaceae, Apocynaceae, Malvaceae, Caryocaraceae, and Arecaceae and combine an overbuilt design (large fruit mass and size) with either a single or few (,3 seeds) extremely large seeds or many small seeds (usually.100 seeds). Within-family and within-genus contrasts between megafaunal and non-megafaunal groups of species indicate a marked difference in fruit diameter and fruit mass but less so for individual seed mass, with a significant trend for megafaunal fruits to have larger seeds and seediness. Conclusions/Significance: Megafaunal fruits allow plants to circumvent the trade-off between seed size and dispersal b

Targeting the hypoxic fraction of tumours using hypoxia activated prodrugs

The presence of a microenvironment within most tumours containing regions of low oxygen tension or hypoxia has profound biological and therapeutic implications. Tumour hypoxia is known to promote the development of an aggressive phenotype, resistance to both chemotherapy and radiotherapy and is strongly associated with poor clinical outcome. Paradoxically, it is recognised as a high priority target and one therapeutic strategies designed to eradicate hypoxic cells in tumours are a group of compounds known collectively as hypoxia activated prodrugs (HAPs) or bioreductive drugs. These drugs are inactive prodrugs that require enzymatic activation (typically by 1 or 2 electron oxidoreductases) to generate cytotoxic species with selectivity for hypoxic cells being determined by (i) the ability of oxygen to either reverse or inhibit the activation process and (ii) the presence of elevated expression of oxidoreductases in tumours. The concepts underpinning HAP development were established over 40 years ago and have been refined over the years to produce a new generation of HAPs that are under preclinical and clinical development. The purpose of this article is to describe current progress in the development of HAPs focusing on the mechanisms of action, preclinical properties and clinical progress of leading examples